| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 3,319,555 | A→G | 18.6% | I247T (ATC→ACC) | ftsH ← | protease, ATP‑dependent zinc‑metallo |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 3,319,555 | 0 | A | G | 18.6% | 32.5 / 4.1 | 22 | I247T (ATC→ACC) | ftsH | protease, ATP‑dependent zinc‑metallo |
| Reads supporting (aligned to +/- strand): ref base A (12/5); new base G (2/2); total (15/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.74e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.60e-01 | |||||||||||
GATAACGATGATACCTTCGTTACCTTCGAAGCCATCCATCTCAACCAGCATCTGGTTCAGAGTCTGTTCACGTTCATCGTGACCACCGCCCAGACCAGCGCCACGCTGGCGGCCTACGGCGTCGATTTCATCGATAAAGATGATGCACGGTGCCGCTTTCTTCGCCTGTTCGAACATGTCACGAACACGGGATGCACCCACACCGACGAACATTTCTACGAAGTCAGAACCGGAGATAGTAAAGAACGGAACTTTCGCTTCGCCTGCAATCGC > CP009273/3319413‑3319685 | gATAACGATGATACCTTCGTTACCTTCGAAGCCATCCATCTCAACCAGCATCTGGTTCAGAGTCTGTTCACGTTCATCGTGACCACCGCCCAGACCAGCGCCACGCTGGCGGCCTACGGCGCCGATCTCATCGAAACAgaagat > 2:164437/1‑144 (MQ=255) atACATTCGTTACCTTCGAAGCCATCCATCTCACCCAGCATCTGGTTCAGAGTCTGTCCACGTTCATCGTGACCACCGCCCAGACCAGCGCCACGCTGGCGGCCTACGACGTCGATTTCGTCGATAAAGATGATGCACGGTGcc > 2:347396/1‑144 (MQ=255) ccTTCGTTACCTTCGAAGCCATCCATCTCAACCAGCATCTGGTTCAGAGTCTGTTGTCTTTCATCGTGACCACCGCCCAGACCAGCGCCCCGCTGGCGGCCTACGGCGTCGATTTCCTCGATAAAGATGATGCACGGTGCCGCTttctt > 1:176582/1‑149 (MQ=255) tCGAAGCCATCCATCTCAACCAGCATCTGGTTCAGAGTCTGTTCACGTTCGTCGTGACCACCGCCCAGACCAGCGCCACGCTGGCGGCCTACGGCGTCGCTTTCATCGATAAAGATGATGCAAGGTGCCGCTTTCTTCGCCTGTTCGaa > 1:273535/1‑149 (MQ=255) ccatccatCTCAACCAGCATCTGGTTCAGAGTCTGTTCACGTTCATCGTGACCACCGCCCAGACCAGCGCCACGCTGGCGGCCTACGGCGTCGATTTCATCGAGAAAGATGCTGCACGGTGCCGCTTTCTTCGCCTGTTCGAACATGTc > 1:152215/1‑149 (MQ=255) tcAACCAGCATCTGGTTCAGAGTCTGTTCACGTTCATCGTGACCACCGCCCAGACCAGCGCCACGCTGGCGGCCTACGGCGTCGATTTCATCGATAAAGATGATGCACGGTGCCGCTTTCTTCGCCTGTTCGAACATGTCACGa > 2:258325/1‑144 (MQ=255) ggttttGGTTCAGGGCCTGTGCGCGGTCATTGTTTTCACTGCCTCGACCAGCGGCACGCTGGCGGTCTACGTCGTCTTGTTCATCGATAAAGATGATGCACGGTGCCGCTTTCTTCGCCTGTTCGAACATGTCACGAACACggg < 2:184207/139‑1 (MQ=255) ggTTCAGAGTCTGTTCACGTTCATCGTGAGCACCGCCCAGACCAGCGCCACGCTGGCGGCCAACGGCGTCGATTTCATCGATAAAGATGATGCACGGTGCCGCTTTCTTCGCCTGTTCGAACAAGTCACGAACACGGGATGCACCccca > 1:243281/1‑149 (MQ=255) tGACCACCGCCCAGACCAGCGCCACGCTGGCGGCCTACGGCGTCGATTTCATCGATACAGATGGTGCACGGTGCCGCTTTCTTCGCCTGTTCGAACATGTCACGAACACGGGATGCACCCACACCGACGAACATTTCTACGAAGTCAGa > 1:156229/1‑149 (MQ=255) tGACCACCGCCCAGACCAGCGCCACGCTGGCGGCCTACGGCGTCGATTTCATCGACAAAGATGGTGCACGGTGCCGCTTTCTTCGCCTGTTCGAACATGTCACGAACCCGGGATGCACCCCCACCGACGAACATTTCTACGGAGTCAGa > 1:79882/1‑149 (MQ=255) accGCCCAGACCAGCGCCACGCTGGCGGCCTACGGCGTCGCTTTCATCGATAATGATGATGCACGGTGCCGCTTTCTTCGCCTGTTCGAACATGTCACGAACACGGGATGCACCCACACCGACGAACATTTCTACGAAGTCAGAACCgg > 1:225771/1‑149 (MQ=255) accGCCCAGACCAGCGCCACGCTGGCGGCCTACGGCGTCGATTTCATCGATAAAGATGATGCACGGTGCCGCTCTCTTCGCCTGTTCGAACATGTCACGAACACGGGATGCACCCACACCGACGAACATTTCTACGAAGTCAGAACCgg > 1:204045/1‑149 (MQ=255) acgcataCCCGCGCCACGCTGGCGGCCTACGGCGCCGATTTCATCTATAAAGATGATGCACGGTGCCGCTTTCTTCGCCTGTTCGAACACGTCACGAACACGGGATGCACCCACACCGACGAACATTTCTACGAAGTCAGAACCGgaga < 1:385709/143‑1 (MQ=255) ccACGCTGGCGGCCTACGGCGTCGATTTCATCGATAAAGATGATGCACGGTGCCGCTTTCTTCGCCTGTTCGAACATGTCACGAACACGGGATGCACCCACACCGACGAACATTTCTACGAAGTCAGAACCGGAGATAGTAAAGAACgg > 1:127133/1‑149 (MQ=255) gCTGGCGGCCTACGGCGTCGATTTCATCGATAAAGATGATGCACGGTGCCGCTTTCTTCGCCTGTTCGAACATGTCACGAACACGGGATGCACCCACACCGACGAACATTTCTACGAAGTCAGAACCGGAGATAGTAAAGAACGGAACt < 1:282257/149‑1 (MQ=255) gcggcCTACGGCGTCGATTTCATCGATAAAGATGATGCACGGTGCCGCTTTCTTCGCCTGTTCGAACATGGCACGAACACGGGATGCACCCACACCGACGAACATTTCTACGAAGTCAGAACCGGAGATAGTCAAGAACGGAACTttcg > 1:364711/1‑149 (MQ=255) cTACGGCGTCGATTTCATCGATAAAGATGGTGCACGGTGCCGCTTTCTTCGCCTGTTCGAACATGTCACGAACACGGGATGCACCCACACCGACGAACATTTCTACGAAGTCAGAACCGGAGATAGTAAAGAACGGAACTttcg < 2:137863/144‑1 (MQ=255) cTACGGCGTCGATTTCATCGATAAAGATGGTGCACGGTGCCGCTTTCTTCGCCTGTTCGAACATGTCACGAACACGGGATGCACCCACACCGACGAACATTTCTACGAAGTCAGAACCGGAGATAGTAAAGAACGGAACTttcg < 2:71352/144‑1 (MQ=255) cGGCGTCGATTTCATCGATAAAGATGATGCACGGTGCCGCTTTCTTCGCCTGTTTTAACATTTCACGAACACGGGATGCACCCACACCGACGAACATTTCTACGAAGTCAGAACCGGAGATAGTAAAGAACGGAACTTTCGCTTCGCCt < 1:289151/149‑1 (MQ=255) cGTCGATTTCATCGATAAAGATGATGCACGGTGCCGCTTTCTTCGCCTGTTCGAACATGTCACGAACACGGGATGCACCCACACCGACGAACATTTCTACGAAGTCAGAACCGGAGATAGTAAAGAACGGAACTTTCGCTTCGCCTGCa > 1:351733/1‑149 (MQ=255) cGTCGATTTCATCGATAAAGATGATGCACGGGTCCGCGTTCTTCGCCTGTTCGAACATGTCACGAACACGGGATGCACCCACACCGACGAACATTTCTACGAAGCCAGAACCGGAGATAGTAACGAACGGAACTctcgcttcgc < 2:86559/144‑1 (MQ=255) tCGATTTCATCGATAAAGATGATGCACGGTGCCGCTTTCTTCGCCTGTTCGAACATGTCACGAACACGGGATGCACCCACACCGACGAACATTTCTACGAAGTCAGAACCGGAGATAGTAAAGAACGGAACTTTCCCTTCGCCTGCAAt > 1:6377/1‑149 (MQ=255) aTCGATAAAGATGATGCACGGTGCCGCTTTCTTCGCCTGTTCGAACATGTCACGAACACGGGATGCACCCACACCGACGAACATTTCTACGAAGTCAGAACCGGAGATAGTAAAGAACGGCCCTTTCGCTTCCCCGGCAATCGc > 2:304318/1‑144 (MQ=255) | GATAACGATGATACCTTCGTTACCTTCGAAGCCATCCATCTCAACCAGCATCTGGTTCAGAGTCTGTTCACGTTCATCGTGACCACCGCCCAGACCAGCGCCACGCTGGCGGCCTACGGCGTCGATTTCATCGATAAAGATGATGCACGGTGCCGCTTTCTTCGCCTGTTCGAACATGTCACGAACACGGGATGCACCCACACCGACGAACATTTCTACGAAGTCAGAACCGGAGATAGTAAAGAACGGAACTTTCGCTTCGCCTGCAATCGC > CP009273/3319413‑3319685 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |