| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 2,493,637 | C→T | 10.5% | T496T (ACC→ACT) | ypdA → | sensor kinase regulating yhjX; pyruvate‑responsive YpdAB two‑component system |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 2,493,637 | 0 | C | T | 10.5% | 94.6 / 3.0 | 38 | T496T (ACC→ACT) | ypdA | sensor kinase regulating yhjX; pyruvate‑responsive YpdAB two‑component system |
| Reads supporting (aligned to +/- strand): ref base C (24/10); new base T (2/2); total (26/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.77e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.73e-01 | |||||||||||
AGGTGAATTGCTGCATTCCCAGCCTGCTGATCCAGCCGTTGGTGGAGAACGCCATTGTCCACGGTATTCAGCCTTGCAAAGGTAAAGGCGTTGTCACCATCAGCGTTGCAGAGTGCGGAAATCGGGTACGCATTGCGGTGCGAGATACCGGGCACGGCATCGATCCAAAGGTGATTGAGCGGGTCGAAGCGAATGAAATGCCGGGCAATAAAATTGGCCTGCTGAATGTCCATCATCGCGTGAAGTTATTGTATGGCGAGGGGCTGCATATCCGCCGCCTGGAGCCGGGGACGGA > CP009273/2493489‑2493783 | agGTGAATTGCTGCATTCCCAGCCTGCTGATCCAGCCGTTGGTGGAGAACGCCATTGTCCAAGGTATTCAGCCTTGCAAAGGTAAAGGCGTTGTCACCATCAGCGTTGCAGAGTGCGGAAATCGGGTACGCATTGCGGTGCGAGATAcc < 1:113129/149‑1 (MQ=255) gctgcATTCCCAGCCTGCTGATCCAGCCGTTGGTGGAGAACGCCATTGTCCACGGTATTCAGCCTTGCAAAGGTAAAGTCGTTGTCACCATCAGCGTTGCAGAGTGCGGAAATCGGGTACGCATTGCGGTGCGAGATACCGGGCACGGc < 1:382957/149‑1 (MQ=255) tgcATTCCCAGCCTGCTGATCCAGCCGTTGGTGGAGAACGCGATTGTACACGGAATTCAGCCTTGCAAAGGTAAAGGCGTTGTCACCATCAGCGTTGCAGAGTGCGGAAATCGGGTACGCATTGCGGTGAGAGATACCGGGGAc > 2:7500/1‑144 (MQ=255) tgcATTCCCAGCCTGCTGATCCAGCCGTTGGTGGAGAAAGCGTTTGTCCACGGTATTCAGCCTTGCAAAGGTAAAGGCGTTGTCACCATCAGCGTTGCAGAGTGCGGAAATCGGGTACGCATTGCGGTGCGAGATACCgggacc > 2:3040/1‑141 (MQ=255) cAGCCTGCTGATCCAGCCGTTGGTGGAGCACGCCATTGTCCACGGTATTCAGCCTTGCAAAGGTAAAGGCGTTGTCACCATCAGCGTTGCAGAGTGCGGAAATCGGGTACGCATTGCGGTGCGAGATACTGGGCACGGCATCGa > 2:170372/1‑144 (MQ=255) cAGCCTGCTGATCCAGCCGTTGGTGGAGAACGCCATTGTCCACGGTAGTCAGCCTTGCAAAGGGAAAGGCGTTGTCACCATCAGCGTTGCAGAGTGCGGAAATCGGGTACGCATTGCGGTGCGAGATACTGGGCACGGCATCGa > 2:170393/1‑144 (MQ=255) gCCTGCTGATCCAGCCGTTGGTGGAGCACGCCATTGTCCACGGTATTCAGCCTTGCAAAGGTAAAGGCGTTGTCACCATCAGGGTTGCAGAGTGCGGAAATCGGGTCCGCATTGCGGTGCGAGATCCCGGGCACGGCATCGATCCAAAg < 1:88947/149‑1 (MQ=255) ccTGCTGATCCAGCCGTTGGTGGAGAACGCCATTGTCCACGGTATTCAGCCTTGCAAAGGTAAAGGCGTTGTCACCATCAGCGTTGCAGAGTGCGGAAATCGGGTACGCATTGCGGTGCGAGATACCGGGCACGGCATCGATCCAAAgg > 1:158747/1‑149 (MQ=255) ccTGCTGATCCAGCCGTTGGTGGAGAACGCCATTGTCCACGGTATTCAGCCTTGCAAAGATAAAGGCGATGACACCATCAGCGTTGCAGAGAGCGGAAAGCGGGTACGCATTGCGGAGGGAGGTTGCGGGGACGGCATCGGTCCAAAgg > 1:254555/1‑149 (MQ=255) aTCCAGCCGTTGGTGGAGAACGCCATTGTCCACGGTATTCAGCCTTGCAAAGGTAAAGGCGTTGTCACCATCAGCGTTGCAGAGTGCGGAAATCGGGTACGCATTGCGGTGCGAGATACCGGGCACGGCATCGATCCAAAgggg > 2:192156/1‑142 (MQ=255) ccAGCCGTTGGTGGAGAACGCCATTGTCCACGGTATTCAGCCTTGCAAAGGTAAAGGCGTTGTCACCATCAGCGTTGCAGAGTGCGGAAATCGGGTACGCATTGCGGGGCGAGATACCGGGCACGGCATCGATCCAAAGGTGATTGAGc > 1:341828/1‑149 (MQ=255) ttGGTGGAGAACGCCATTGTCCACGGTATTCAGCCTTGCAAAGGTAAAGGCGTTGTCACCATCAGCGTTGCAGAGTGCGGAAATCGGGTACGCATTGCGGTGCGAGAGACCGGGCACGGCATCGATCCAAAGGGGATTGAGCGGGTCGa > 1:348771/1‑149 (MQ=255) gtggAGAACGCCATTGTCCACGGTATTCAGCCTTGCAAAGGTAAAGGCGTTGTCACCATCAGCGTTGCAGAGTGCGGAAATCGGGTACGCATTGCGGTGCGAGATACCGGGCACGGCATCGATCCAAAGGTGATTGAGCGGGTc < 2:233466/144‑1 (MQ=255) tggAGAACGCCATTGTCCACGGTATTCAGCCTTGCAAAGGTAAAGGCGTTGTCACCATCAGCGTTGCAGAGTGCGGAAATCGGGTACGCATTGCGGTGCGAGATACCGGGCACGGCATCGATCCAAAGGTGATTGAGCGGGTCg > 2:113392/1‑144 (MQ=255) gagaACGACCTTGTCCCCGGTTTTCAGCCTTGCAAAGGAAAAGGCGTTGTCACCATCAGCGTTGCAGAGTGCGGAAATCGGGTACGCATTGCGGTGCGAGATACCGGGCACGGCATCGATCCAAAGGTGATTGAGCGGGTCGAAGCGaa < 1:324017/149‑1 (MQ=255) gCCATTGTCCACGGTATTCAGCCTTGCAAAGGTAAAGGCGTTGTCACCATCAGCGTTGCAGAGTGCGGAAATCGGGTACGCATTGCGGTGCGAGATACCGGGCACGGCATCGATCCACAGGTGATTGAGCGGGTCGAAGCGAAt > 2:335846/1‑144 (MQ=255) tGTCCACGGGATTCAGCCTTGCAAAGGTAAAGGCGTTGTCACCATCAGCGTTGCAGAGTGCGGAAATCGGGTACGCATTGCGGTGCGAGATACCGGGCACGGCATCGATCCAAAGGTGATTGAGCGGGTCGAAGCGAATGAAAt < 2:176824/144‑1 (MQ=255) gTATTCAGCCTTGCAAAGGTAAAGGCGTTGTCACCATCAGCGTTGCAGAGTGCGGAAATCGGGTACGCATTGCGGTGCGAGATACCGGGCACGGCATCGATCCAAAGGTGATTGAGCGGGTCGAAGCGAATGAAATGCCGTGCa > 2:124646/1‑144 (MQ=255) tCAGCCTTGCAAAGGTAAAGGCGTTGTCACCATCCGCGTTGCAGAATGCGGAAATCGGGTACGCATTGCGGTGCGAGATACCGGGCACGGCATCGATCCAAAGGTGATTGAGCGGGTCGAAGCGAATGAAATGCCGGGCAATAAAATTg > 1:176916/1‑149 (MQ=255) tCAGCCTTGCAAAGGTAAAGGCGTTGTCACCATCAGCGTTGCAGAGTGCGGAAATCGGGTACGCATTGCGGTGCGAGATACCGGGCACGGCATCGATCCAAAGGTGATTGAGCGGGTCGAAGCGAATGAAATGCCGGGCAATAAAATTg > 1:176940/1‑149 (MQ=255) ccTTGCAAAGGTAAAGGCGTTGTCACCATCAGCGTTGCAGAGTGCGGAAATCGGGTACGCATTGCGGTGCGAGATACCGGGCACGGCATCGATCCAAAGGTGATTGAGCGGGTCGAAGCGAATGAAATGCCGGGCAATAAAATTGGCct > 1:221891/1‑149 (MQ=255) tGCAAAGGTAAAGGCGTTGTCCCCATCAGCGTTGCAGAGTGCGGAAACCGGGTACGCATTGCGGTGCGAGAGACCGGGCACGGCATCGATCCAAAGGTGATTGAGCGGGTCGAAGCGAATGAAATGCCGGGCAATAAAATTGGc < 2:353447/144‑1 (MQ=255) cAAAGGTAAAGGCGTTGTCACCATCAGCGTTGCAGAGTGCGGAAATCGGGTACGCATTGCGGTGCGAGATACCGGGCACGGCATCGCTCCAAAGGTGATTGAGCGGGTCGAAGCGAATGAAATGCCGGGCAATAAAATTGGCCTGCTGa < 1:77401/149‑1 (MQ=255) aaaGGTAAAGGCGTTGTCACCATCAGCGTTGCAGAGTGCGGAAATCGGGTACGCATTGCGGTGCGAGATACCGGGCACGGCATCGATCCAAAGGTGATTGAGCGGGTCGAAGCGAATGAAATGCCGGGCAATAAAATTGGCCTGCTGaa > 1:290621/1‑149 (MQ=255) aaaGGCGTTGTCACCATCAGCGTTGCAGAGTGCGGAAATCGGGTACGCATTGCGGTGCGAGATACCGGGCACGGCATCGATCCAAAGGTGATTGAGCGGGTCGAAGCGAATGAAATGCCGGGCAATAAAATTGGCCTGCAGAAt > 2:11942/1‑144 (MQ=255) aaGGCGTTGTCACCATCAGCGTTGCAGGGTGCGGAAATCGGGTACGCTTTGCGGTGCGGGATACCGGGCACGGCATCGATCCAAAGGTGATTGAGCGGGTCGAAGCGAATGAAATGCCGGGCAATACAATTGGCCTGCTGAATGTCcat < 1:58503/149‑1 (MQ=255) ttGTCACCATCAGCGTTGCAGAGTGCGGAAATCGGGTACGCATTGCGGTGCGAGATACCGGGCACGGCATCGATCCAAAGGTGATTGAGCGGGTCGGTGCGAATGAAATGCCGGGCAATAAAATTGGCCTGCTGAATGTCcatc > 2:342978/1‑144 (MQ=255) ttGTCACCATCAGCGTTGCAGAGTGCGGAAATCGGGTACGCATTGCGGTGCGAGATACCGGGCACGGCATCGATCCAAAGGTGATTGAGCGGGTCGAAGCGAATGAAATGCCGGGCAATAACGATGGACGGCTGAATGTCcatc > 2:266432/1‑144 (MQ=255) aCCATCAGCGTTGCAGAGTGCGGAAATCGGGTACGCATTGCGGTGCGAGATACCGGGCACGGCATCGATCCAAAGGTGATTGAGCGGGTCGAAGCGAATGAAATGCCGGGCAATAAAATTGGCCTGCTGAATGTCCATCATAGCGTGaa > 1:385843/1‑149 (MQ=255) aGCGTTGCAGAGTGCGGAAATCGGGTACGCATTGCGGTGCGAGATACCGGGCACGGCATCGATCCAAAGGTGATTGAGCGGGTCGAAGCGAATGAAATGCCGGGCAATAAAATTGGCCTGCTGAATGTCCATCATCGCGTGAAGTTAtt > 1:309294/1‑149 (MQ=255) ggAAATCGGGTACGCATTGCGGTGCGAGATACTGGGCACGGCATCGATCCAAAGGTGATTGAGCGGGTCGAAGCGAATGAAATGCCGGGCAATAAAATTGGCCTGCTGAATTTCCATCATCGCGTGAAGTTATTGTATGGCGAGGGGCt < 1:193952/149‑1 (MQ=255) ggAAATCGGGTACGCATTGCGGTGCGAGATACCGGGCACGGCATCGATCCAAAGGTGATTGAGCGGGTCGAAGCGAATGAAATGCCGGGCAATAAAATTGGCCTGCTGAATGTCCATCATCGCGTGAAGTTATTGTATGGCGAg > 2:21161/1‑144 (MQ=255) ggAAATCGGGTACGCATTGCGGTGCGAGATACCGGGCACGGCATCGATCCAAAGGTGATTGAGCGGGTCGAAGCGAATGAAATGCCGGGCAATAAAATTGGCCTGCTGAATGTCCATCATCGCGTGAAGTTATTGTATGGCGAGGGGCt > 1:313163/1‑149 (MQ=255) ggAAATCGGGTACGCATTGCGGTGCGAGAGACTGGGCACGGCATCGATCCAAAGGTGATTGAGCGGGTCGAAGCGAATGAAATGCCGGGCAATAAAATTGGCCTGATGAGCGTCCATCATCGCGTGAAGTTATTGTATGGCGAGGGGCt < 1:193980/149‑1 (MQ=255) cGGGGAGGCATGGCGGTGCGAGATACCGGGCACGGCATCGATCCAAAGGTGATTGAGCGGGTCGAAGCGAATGAAATGCCGGGCAATAAAATTGGCCTGCTGAATGTCCATCATCGCGTGAAGTTATTGTATGGCGAGGGGCTg < 2:53367/144‑1 (MQ=255) ttctctttGCGGTGCGAGATACCGGGCACGGCATCGATCCAAAGGTGATTGGGCGGGTCGAAGCGAATGAAATGCCGGGCAATAAAATTGGCCTGCGGAATGTCCATCATCGCGTGAAGTTATGGTATGGCGGGGGGCTGCata > 2:148501/7‑144 (MQ=255) tACGCAGTGCGGTGCGAGATACCGGGCACGGCATCGATCCAAAGGTGATTGAGCGGGTCGAAGCGAATGAAATGCCGGGCAATAAAATTGGCCTGCTGAATGTCCATCATCGCGTGAAGCTATTGTATGGCGACGGGCTGCcta > 2:148443/1‑144 (MQ=255) gCATTGCGGTGCGAGATACCGGGCACGGCATCGATCCAAAGGTGATTGAGCGGGTCGAAGCGAATGAAATGCCGGGCAATAAAATTGGCCTGCTGAATGTCCATCATCGCGTGAAGTTATTGTATGGCGAGGGGCTGCATATCcgccgc > 1:215462/1‑149 (MQ=255) aCCGGGCACGGCATCGATCCAAAGGTGATTGAGCGGGTCGAAGCGAATGAAATGCCGGGCAATAAAATTGGCCTGCTGAATGTCCATCATCGCGTGAAGTTATTGTATGGCGAGGGGCTGCATATCCGCCGCCTGGAGCCGGGGACGGa < 1:357138/149‑1 (MQ=255) | AGGTGAATTGCTGCATTCCCAGCCTGCTGATCCAGCCGTTGGTGGAGAACGCCATTGTCCACGGTATTCAGCCTTGCAAAGGTAAAGGCGTTGTCACCATCAGCGTTGCAGAGTGCGGAAATCGGGTACGCATTGCGGTGCGAGATACCGGGCACGGCATCGATCCAAAGGTGATTGAGCGGGTCGAAGCGAATGAAATGCCGGGCAATAAAATTGGCCTGCTGAATGTCCATCATCGCGTGAAGTTATTGTATGGCGAGGGGCTGCATATCCGCCGCCTGGAGCCGGGGACGGA > CP009273/2493489‑2493783 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |