Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP009273	2,083,992	C→A	S107* (TCG→TAG)	hisG →	ATP phosphoribosyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	2,083,992	0	C	A	100.0%	102.1 / NA	36	S107* (TCG→TAG)	hisG	ATP phosphoribosyltransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (15/21);  total (15/21)

GGTCTGGTAATGGATGGCGTGGTAGACCTTGGGATTATCGGCGAAAACGTGCTGGAAGAAGAGCTGCTTAACCGCCGCGCCCAGGGTGAAGATCCACGCTACTTTACCCTGCGTCGTCTGGATTTCGGCGGCTGTCGTCTTTCGCTGGCAACGCCGGTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCTCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGTTCTGTT  >  CP009273/2083850‑2084137                                                                                                                                               |                                                                                                                                                  ggTCTGGTAATGGATGGCGTGGTAGACCTTGGGATTATCGGCGAAAACGTGCTGGAAGAAGAGCTGCTTAACCGCCGCGCCCAGGGTGAAGATCCACGCTACTTTACCCTGCGTCGTCTGGATTTCGGCGGCTGTCGTCTTTAGCTGGc                                                                                                                                             <  1:211633/149‑1 (MQ=255)   tCTGGTAATGGATGGCGTGGTAGACCTTGGGATTATCGGCGAAAACGTGCTGGAAGAAGAGCTGCTTAACCGCCGCGCCCAGGGTGAAGATCCACGCCACTTTACCCTGCGTCGTCTGGATTTCGGCGGCTGTCGTCTTTAGCTGGCaa                                                                                                                                           <  2:946402/149‑1 (MQ=255)       gTAATGGATGGCGTGGTAGACCTTGGGATTATCGGCGAAAACGTGCTGGAAGAAGAGCTGCTTAACCGCGGCGCCCAGGGTGAAGATCCACGCTACTTTACCCTGCGTCGTCTGGATTTCGGCGGCTGGCGTCTTTAGCTGGCAACGcc                                                                                                                                       >  2:793074/1‑149 (MQ=255)       gTAATGGATGGCGTGGTAGACCTTGGGATTATCGGCGAAAACGTGCTGGAAGAAGAGCTGCTTAACCGCCGCGCCCAGGGTGAAGATCCACGCTACTTTACCCTGCGTCGTCTGGATTTCGGCGGCTGTCGTCTTTAGCTGGCAACGcc                                                                                                                                       >  1:47072/1‑149 (MQ=255)        tcatggattgCGTGGTAGACCTTGGGATTATCGGCGAAAACGTGCTGGAAGAAGAGCTGCTTACCCGCCGCGCCCAGGGTGCAGATCCACGCTACTTTACCCTGCGTCGTCTGGATTTCGGCGGCTGTCGTCTTTAGCTGGCAACGCCg                                                                                                                                      <  1:157499/147‑1 (MQ=255)          agggatggCGTGGTGGACCTTGGGACTATCGGCGAAACCGTTCTGGAGGAAGAGCTCCTTAACCGCCGCGCCCATGGTGTACACCCTCGCTACTGTACCCGGCGTCGCCGGGATTTCGGCGGCTGTCGTCTTTAGCTGGCAACGCCGGt                                                                                                                                    <  2:35043/147‑1 (MQ=255)           tggatggCGTGGTAGACCTTGGGATTATCGGCGAAAACGTGCTGGAAGAAGAGCTGCTTAAGCGCCGCGCCCAGGGTGAAGATCCACGCTACTTTACCCTGCGTCGTCTGGATTTCGGCGGCTGTCGTCTTTAGCTGGCAACGCCGGtt                                                                                                                                   <  2:719115/149‑1 (MQ=255)           gcgatggCTTGGTATACCTTGGGATTATCGGCGAAAACGTGCTGGAAGAAGGTCTGCCTAACCGCCGCGCCCAGGTTGAAGATCCCCGCTACTTTACCCTGCGTCGTCTGGATTTCGGCGGCTGTCGTCTTTAGCTGGCAACGCCGGtt                                                                                                                                   <  2:644587/147‑1 (MQ=255)               tggCGTGGTAGACCTTGGGATTATCGGCGAAAACGTGCTGGAAGAAGAGCTGCTTAACCGCCGCGCCCAGGGTGAAGATCCACGCTACTTTACCCTGCGTCGTCTGGATTTCGGCGGCTGTCGACCTTAGCTGGCCACGCCGGTtgatg                                                                                                                               >  2:153626/1‑149 (MQ=255)                  cGTGGTAGACCTTGGGATTATCGGCGAAAACGTGCTGGAAGAAGAGCTGCTTAACCGCCGCGCCCAGGGTGAAGATCCACGCTACTTTACCCTGCGTCGTCTGGAGTTCGGCGGCTGTCGTCTTTAGCTGGCAACGCCGGTTGATGAAg                                                                                                                            >  2:367148/1‑149 (MQ=255)                                     aTCGGCGAAAACGTGCTGGAAGAAGAGCTGCTTAACCGCCGCGCCCAGGGTGAAGATCCACGCTACTTTACCCTGCGTCGTCTGGATTTCGGCGGCTGTCGTCTTTAGCTGGCAACGCCGGTTGATGAAGCCTGGGACGGTCCGCtctc                                                                                                         <  1:215242/149‑1 (MQ=255)                                           gAAAACGTGCTGGAAGAAGAGCTGCTTAACCGCCGCGCCCAGGGTGAAGATCCACGCTACTTTACCCTGCGTCGTCTGGATTTCGGCGGCTGTCGTCTTTAGCTGGCAACGCCGGTTGGTGACGCCTGGGACGGTCCGCTCTCCTTcaa                                                                                                   >  1:81585/1‑149 (MQ=255)                                                  tGCTGGCAGAAGGGCTGCTTAACCGCCGCGCCCAGGGTGAAGATCCACGCTACTTTACCCTGCGTCGTCGCGATTTCGGCAGATCTCTTCTTTAGCTGGCAACGCCGGTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTaaa                                                                                            <  1:461014/149‑1 (MQ=255)                                                     tGGAAGAAGAGCTGCTTAACCGCCGCGCCCAGGGTGAAGATCCACGCTACTTTACCCTGCGTCGTCTGGATTTCGGCGGCTGTCGTCTTTAGCTGGCAACGCCGGTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGt                                                                                         >  2:767695/1‑149 (MQ=255)                                                     gggAAGAAGAGCTGCTTAACCGCCGCGCCCAGGGTGAAGATCCACGCTACTTTACCCTGCGTCGTCTGGATTTCGGCGGCTGTCGTCTTTAGCTGGCAACGCCGGTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGt                                                                                         <  1:92081/148‑1 (MQ=255)                                                      ggAAGAAGAGCTGCTTAACCGCCGCGCCCAGGGTGAAGATCCACGCTACTTTACCCTGCGTCGTCTGGATTTCGGCGGCTGTCGTCTTTAGCTGGCAACGCCGGTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTa                                                                                        >  2:232169/1‑149 (MQ=255)                                                          gaagaGCTGCTTAACCGCCGCGCCCAGGGTGCAGATCCACGCTACTTTACCCTGCGTCGTCTGGATTTCGGCGGCTGTCGTCTTTAGCTGGCAACGCCGGTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGc                                                                                    >  2:699818/1‑149 (MQ=255)                                                          gaagaGCTGCTTAACCGCCGCGCCCAGGGTGAAGATCCACGCTACTTTACGCTGCGGCGTCTGGATGTCGGCGGCTGTCGTCTTTAGCTGGCAACGCGGGTTGATGGAGCCGGGGGCGGTCCGCTCTCCAGAAACGGTAAACGGATCGc                                                                                    >  2:870016/1‑149 (MQ=255)                                                           aagaGCTGCCTAACCGCCGCGCCCAGGGTGAAGATCCACGCTACTTTACCCTGCGTCGTCTGGATTTCGGCGGCTGGCGTCTTTAGCTGGCAACGCCGGTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGcc                                                                                   <  1:378050/149‑1 (MQ=255)                                                                ctgctTAACCGCCGCGCCCAGGGTGAAGATCCACGCTACTTTACCCTGCGTCGTCTGGATTTCGGCGGCTGTCGTCTTTAGCTGGCAACGCCGGTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACctc                                                                              >  1:641034/1‑149 (MQ=255)                                                                        ccgccgCGCCCAGGGTGAAGATCCACGCTACTTTACCCTGCGTCGTCTGGATTTCGGCGGCTGTCGTCTTTAGCTGGCAACGCCGGTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCTc                                                                      <  1:420441/149‑1 (MQ=255)                                                                                      gTGAAGATCCACGCTACGTTGCCCGGCGTCGTCGGGATTTCGGGGGCTGTCGTCTTTAGCTGGCAACGCCGGGTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCTCACCTGCTCAAGCGt                                                        <  2:43695/149‑1 (MQ=255)                                                                                            aTCCACGCTACTTTACCCTGCGTCGTCTGGATTTCGGCGGTTGTCGTCTTTAGCTGGCAACGCCGGTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCTCACCTGCTCCAGCGTTAtctc                                                  >  2:894615/1‑149 (MQ=255)                                                                                               cACGCTACTTTACCCTGCGTCGTCTGGATTTCGGCGGCTGTCGTCTTTAGCTGGCAACGCCGGTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCTCACCTGCTCAAGCGTTATCTCGAc                                               >  2:726710/1‑149 (MQ=255)                                                                                               cACGCGACTGTCCCCTGCGTCGTGTGGGTTTCGGGGGCTGGCGTCTTTAGCTGGCAACGCCGGTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCTCACCTGCTCAAGCGTTATCTCGAc                                               <  2:228142/149‑1 (MQ=255)                                                                                                   cTACTGTACCCGTCGTCGTCTGGATTTCGGCGGCTGTCGTCTTTAGCTGGCAACGCCGGTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCTCACCTGCTCAAGCGTTATCTCGACCAGa                                           <  1:141733/149‑1 (MQ=255)                                                                                                        ttACCCTGCGTCGTCTGGATTTCGGCGGCTGTCGTCTTTAGCTGGCAACGCCGGTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCTCACCTGCTCAAGCGTTATCTCGACCAGAAAGGc                                      <  1:709637/149‑1 (MQ=255)                                                                                                        ttACCCTGCGTCGTCTGGATTTCGGCGGCTGTCGTCTTTAGCTGGCAACGCCGGTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCTCACCTGCTAAAGCGTTATCTCGACCAGAAAGGc                                      <  1:709623/149‑1 (MQ=255)                                                                                                                      cTGGGTTTCGGCGGCGGTCGTCTTAAGCTGGCAACGCCGGTTGATGAATCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTACCGCCACCTCTTATCCTCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATc                        <  2:930752/149‑1 (MQ=255)                                                                                                                      cTGGATTTCGGCGGCTGTCGTCTTTAGCTGGCAACGCCGTTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCTCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATc                        <  1:236840/149‑1 (MQ=255)                                                                                                                      cTGGATTTCGGCGGCTGTCGTCTTTAGCTGGCAACGCCGGTTGATGACGCCTGGTACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCTCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATc                        <  1:181813/149‑1 (MQ=255)                                                                                                                        ggATTTCGGCGGCTGTCGTCTTTAGCTGGCAACGCCGGTTGATGAAGCCTGGGACGGTCAGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCTCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCCTTTAAATCCt                      >  2:52298/1‑149 (MQ=255)                                                                                                                         gATTTCGGCGGCTGTCGTCTTTAGCTGGCAACGCCGGTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCTCACCTGCTCAAGCGTTATCTCGACCAGCAAGGCATCTCTTTTAAATCCTg                     >  1:135415/1‑149 (MQ=255)                                                                                                                         gATTTCGGCGGCTGTCGTCTTTAGCTGGCAACGCCGGTTGATGAAGCCTGGGACGGTCCGCGCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCTCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTg                     <  1:14533/149‑1 (MQ=255)                                                                                                                                gcggcTTGCTTCTGTAGCCGGCAACGGCGGTTGCTGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCTCACCTGCTCAAGCGTTATCTCGACCCGAAAGGCATCTCTTTTAAATCCTGCTTACTg              <  1:152237/149‑1 (MQ=255)                                                                                                                                            tttAGCTGGCAACGCCGGTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCTCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGTTCTGtt  >  1:727362/1‑149 (MQ=255)                                                                                                                                               |                                                                                                                                                  GGTCTGGTAATGGATGGCGTGGTAGACCTTGGGATTATCGGCGAAAACGTGCTGGAAGAAGAGCTGCTTAACCGCCGCGCCCAGGGTGAAGATCCACGCTACTTTACCCTGCGTCGTCTGGATTTCGGCGGCTGTCGTCTTTCGCTGGCAACGCCGGTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCTCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGTTCTGTT  >  CP009273/2083850‑2084137

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑