Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP009273	982,560	T→A	intergenic (‑122/+481)	ompF ← / ← asnS	outer membrane porin 1a (Ia;b;F)/asparaginyl tRNA synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	982,560	0	T	A	100.0%	88.4 / NA	29	intergenic (‑122/+481)	ompF/asnS	outer membrane porin 1a (Ia;b;F)/asparaginyl tRNA synthetase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (14/15);  total (14/15)

TGCCAGAATATTGCGCTTCATCATTATTTATTACCCTCATGGTTTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCATATTTCCATAGAGACGTGATGATATTTAC  >  CP009273/982415‑982704                                                                                                                                                  |                                                                                                                                                 tGCCAGAATATTGCGCTTCATCATTATTTATTACCCTCATGGTTTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTACCa                                                                                                                                               <  1:526323/149‑1 (MQ=255) tGCCAGAATATTGCGCTTCATCATTATTTATTACCCTCATGGTTTTTTTTATGACACCTGCCACTGACGTGCATCAGATACGTCCATAAAAATTTGCGGAACTACTGATGAGAGTTTGGTGCCTTTATGAGCCCGCCAGCATCTTACCa                                                                                                                                               >  2:819160/1‑149 (MQ=255)                         tatttattACCCTCATGGTTTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTACCATTCAAACTAACGTTTCGTTACCGt                                                                                                                       >  2:744876/1‑149 (MQ=255)                                taCCCTCATGGTTTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCGGGCATCTTAGCAGTCAAACTAACGTTTCGCTACCGTGAAAGTg                                                                                                                <  2:679970/149‑1 (MQ=255)                                      cttGGTTTTTTCTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTACCATTCCACCTGACGTTTCGCTACCGTGAAAGTGCTACaa                                                                                                          <  1:510218/147‑1 (MQ=255)                                          gTTTTTTTTATGACCCCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTACCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACaaagat                                                                                                      <  2:639515/149‑1 (MQ=255)                                              tttttATGACACCTTCCATTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTACCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACaaagataaag                                                                                                  <  2:123948/149‑1 (MQ=255)                                                        acCGGCCCCTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTACCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTgtacgg                                                                                        <  1:258685/149‑6 (MQ=255)                                                               aCTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAAGTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTACCATTCAAACTAAAGTTTCGTTACCGTGAAAGTGATAAAAAGATAAAGATTTAGTTTCAAAAAGa                                                                                 >  2:920963/1‑149 (MQ=255)                                                                       cAATAAGTTCTGGCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTACCATTCAAACTAACGTTGCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAatat                                                                         <  1:13133/149‑1 (MQ=255)                                                                         aTAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTACCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGt                                                                       >  2:754555/1‑149 (MQ=255)                                                                          tAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTACCATTCAAACTAACGTTTCGCTACAGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTa                                                                      >  2:32911/1‑149 (MQ=255)                                                                            aGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTACCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAAc                                                                    <  1:444352/149‑1 (MQ=255)                                                                                      aTAAAAATTTACGGAACTATTGAGGAGAGTTTGGTGTCGTTAGTTGTCGGCAGGCATCTTACCATTCAAAGTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTaaa                                                          <  2:866147/149‑1 (MQ=255)                                                                                       tAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTACCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTaaaa                                                         >  2:313524/1‑149 (MQ=255)                                                                                        aaaaaTTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTACCATTCAAACTAACGTTTCGCTACCGTGAAAGGGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTAAAAt                                                        <  1:462804/149‑1 (MQ=255)                                                                                                aCGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTACCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTAAGGaa                                                >  1:275700/1‑149 (MQ=255)                                                                                                  ggAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTACCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTAAGGaaat                                              >  1:372587/1‑147 (MQ=255)                                                                                                      cTAATGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCGTACCATTCAAACAAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGAGTTGGTAAAAAAAAAAAAAAATATGTAACCAGAGGTAAAATTTAAGGAACTTtgt                                          <  2:684198/149‑1 (MQ=255)                                                                                                              gagaGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTACCATTCAAACTAACGCTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCg                                  <  1:806887/149‑1 (MQ=255)                                                                                                                   tttGGTGTCTTAATGTGTCTGCAGGCATCTTAACATTCAAACTAATGTTTCGCTCCCGTGAAAGTGCTACAAAGATAAAGATCTGGTTTAAAAAAGGATAAGTAAGTAACTGAAAGTAAAAGTTAAGGAACTTTGTGAACAACGTCata                             >  2:335810/1‑149 (MQ=255)                                                                                                                       gtgtCTTTATGTGTCTGCAGGCATCTTACCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCATATTTc                         >  1:255390/1‑149 (MQ=255)                                                                                                                                  tgtCTGCAGGCATCTTACCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTACGGAACTTTGTGAACACCGTCATATTTCCATAGAGACGt              >  1:488535/1‑149 (MQ=255)                                                                                                                                     cTGCAGGCATCTTACCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCATATTTCCATAGAGACGtgat           <  1:189395/149‑1 (MQ=255)                                                                                                                                     cTGCAGGCATCTTACCATTCAAACTAACGTCTCGCTACCCTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTAAGGCACTTTGTGAACACCGTCATATTTCCATAGAGACGtgat           <  2:381697/149‑1 (MQ=255)                                                                                                                                        cAGGCATCTTACCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCATATTTCCATAGAGACGTgatgat        >  1:844297/1‑149 (MQ=255)                                                                                                                                         aGGCATCTTACCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCATATTTCCATAGAGACGTGATGata       >  1:750022/1‑149 (MQ=255)                                                                                                                                         aGGCATCTGAGCAGTAAAACTAACGTTTCGCGACCGTGAAAGCGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCATATTTCCATAGAGACGTGATGata       <  2:838277/149‑1 (MQ=255)                                                                                                                                              tCTTACCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCATATTTCCATAGAGACGTGATGATATTTAc  >  1:660854/1‑149 (MQ=255)                                                                                                                                                  |                                                                                                                                                 TGCCAGAATATTGCGCTTCATCATTATTTATTACCCTCATGGTTTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCATATTTCCATAGAGACGTGATGATATTTAC  >  CP009273/982415‑982704

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑