BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP009273	4,390,235	T→A	I44N (ATC→AAC)	hfq →	global sRNA chaperone; HF‑I, host factor for RNA phage Q beta replication

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	4,390,235	0	T	A	100.0%	390.3 / NA	118	I44N (ATC→AAC)	hfq	global sRNA chaperone; HF‑I, host factor for RNA phage Q beta replication
Reads supporting (aligned to +/- strand): ref base T (0/0); new base A (60/58); total (60/58)

Only 100 of 118 total aligned reads displayed.

AGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCTCATCACAGTAACAACGCCGGTGGCGGTACCAGCAGTAACTACCATCATGGTAGCAGCGCGCAGAA  >  CP009273/4390092‑4390376

 ggAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGAACCTGtt                                                                                                                                         >  1:1718039/1‑149 (MQ=255)

 ggAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGAACCTGtt                                                                                                                                         >  1:1082728/1‑149 (MQ=255)

  gAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGAGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGAAAATAGAGTATTTTGATCAGTTGGGGAACCTGTTg                                                                                                                                        >  1:118386/1‑149 (MQ=255)

   aaaaGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGAAcc                                                                                                                                             >  2:528184/1‑143 (MQ=255)

       gagagaATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGAACCTGtt                                                                                                                                         <  2:739257/143‑1 (MQ=255)

        agagaATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGAACCTGTTg                                                                                                                                        >  2:808001/1‑143 (MQ=255)

          agaATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGAACCTGTTGAAAAacac                                                                                                                                >  1:1179017/1‑149 (MQ=255)

           gaATGGCTAATGGGCAATTTTTACAAGTTCCGTTCCTGAACGAACTGCTTCGGGAACGTGTACCAGCTGCTATTTATTTGGTGAATGGTATTAAGCCGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGAACCTGTTGAAAAACACg                                                                                                                               <  1:15571/149‑1 (MQ=255)

           gaATGGCTAAGGGGCAATCTTTACAAGATCCGTTACTGAACGCACAGCGACGGGAACGTGTTCCTGTTTCTATTTATTTGGTTAATGGCATTAAGCTGCAAGGGCTAACGAAGTCGTTTGATCAGTTCGTGAACCTGGTGaaa                                                                                                                                     >  2:545934/1‑143 (MQ=255)

            aaTGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGAACCTGTTGaaaa                                                                                                                                    >  2:943380/1‑143 (MQ=255)

                  tAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGGGTCTTTTGATCAGTCCGTGAACGTGTTGAAAAGCACGGTCTGca                                                                                                                        >  1:504639/1‑148 (MQ=255)

                    aGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGAACCTGTTGAAAAACACGGTc                                                                                                                            <  2:1096644/143‑1 (MQ=255)

                     ggggCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGTGAACGTGTTCCAGTTTCTATTTATTTGGTCACTGGTATTAAGCTGCTAGGGCAAATCGAGTCTTTTGATCAGTTCGTGAACCTGTTGAAAAACACGGTCa                                                                                                                           <  2:955515/143‑1 (MQ=255)

                       ggCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGTGCAAATCGAGTCTTTTGATCAGTTCGTGAACCTGTTGAAAAACACGGTCAGc                                                                                                                         <  2:199084/143‑1 (MQ=255)

                       ggCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATAGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGAACCTGTTGAAAAACACGGTCAGc                                                                                                                         <  2:1178225/143‑1 (MQ=255)

                       ggCAATCTTTACAAAATCCGTTCCTGAACGGACTGCGTCGGGAACGTGTTGCAGTTTCTATTGATTTGGTGAATGGTATTAAGCTGCAAGGGCAAACCGAGTCTTTTGATCAGTTCGGGAACCTGTTGAAAAACACGGTCGGc                                                                                                                         >  2:845668/1‑143 (MQ=255)

                        gCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGAACCTGTTGAAAAACACGGTCAGcc                                                                                                                        >  2:605283/1‑143 (MQ=255)

                          aaTCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGAACCTGTTGAAAAACACGGTCAGCCAg                                                                                                                      >  2:524615/1‑143 (MQ=255)

                            tttttACAAGCGCCCGTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGAACCTGTTGAAAAACACGGTCAGCCAGATGGTTTa                                                                                                              <  1:1598323/147‑1 (MQ=255)

                              tttACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGAACCTGTTGAAAAACACGGTCAGCCAGATGGTTTACa                                                                                                            <  1:423150/149‑1 (MQ=255)

                                tACCAGATCCGCTCCCGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGAACCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAg                                                                                                          <  1:578776/149‑1 (MQ=255)

                                tACAAGATCCGTTCCTGAACGCACGGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGAACCTGTTGAAAAACACGGTCAGCCAGATGGtt                                                                                                                <  2:149492/143‑1 (MQ=255)

                                  cAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGAACCTGTTGAAAAACACGGTCAGCCAGATGGTTTa                                                                                                              <  2:1513450/143‑1 (MQ=255)

                                   aaGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGAACCTGTTGAAAAACACGGTCAGCCAGATGGTTTAc                                                                                                             >  2:542697/1‑143 (MQ=255)

                                   aaGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGAACCTGTTGAAAAACACGGTCAGCCAGATGGTTTAc                                                                                                             <  2:834886/143‑1 (MQ=255)

                                    aGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTCCGATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGAACCTGTTGAAAAGCACGGTCAGCCAGATGGTTTACa                                                                                                            <  2:1197330/143‑1 (MQ=255)

                                     gATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATCGGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGAACCTGTTGAAAAACACGGTCGGCCCGATGGTTTACaa                                                                                                           >  2:1149791/1‑143 (MQ=255)

                                      aTCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAGTCGAGTCTTTTGATCAGTTCGTGAACCTGTTGAAAAAAACGGTCAGCCAGATGGTTTACCAg                                                                                                          >  2:936441/1‑143 (MQ=255)

                                         cGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGAACCTGTTGAAAAACACGGTCAGCCAGATGGCTTACAAGCACGCGAtt                                                                                                 <  1:1204232/149‑1 (MQ=255)

                                           ttCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGCATGGGATTAAACTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGAACCTGTTGAAAAACACGGTCAGCCAGATGGTTTCCAAGCACGCGATTTc                                                                                               >  1:62692/1‑149 (MQ=255)

                                           ttCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGAACCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCAcgc                                                                                                     <  2:1121091/143‑1 (MQ=255)

                                           ttCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGAACCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTc                                                                                               >  1:1878713/1‑149 (MQ=255)

                                             ccTGAACGCACTGCGTCGGTAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGAACCTGTTGAAAAACACGGGCAGCCAGATGGTTTACAAGCACGCGATTTCTa                                                                                             <  1:1783406/149‑1 (MQ=255)

                                             ccTGAACGCACTGCATCGGGAACGTATTCCAGTCTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGAGCAGTTCGGGAACCTGTTGAAAAACACGGTCATCCAGATGGTTTACAAGCACGCGATTTCTa                                                                                             <  1:1166192/149‑1 (MQ=255)

                                               tGAACGCACTGCGTCGGGAACGGGTTCCAGTTTCTATTTATTTGGTGAATGGTAATAAGCTGCAAGGGCAAATCGAGTCCTTTGATCAGTTCGTGAACCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACt                                                                                           <  1:75700/149‑1 (MQ=255)

                                                gAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGAACCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGAttt                                                                                                <  2:835257/143‑1 (MQ=255)

                                                  aCGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGAACCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACtgtt                                                                                        <  1:348439/149‑1 (MQ=255)

                                                   cGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTGATGTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGAACCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACtgttg                                                                                       >  1:1315387/1‑149 (MQ=255)

                                                     cACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGAACCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTc                                                                                     <  1:812894/149‑1 (MQ=255)

                                                       cTGCGTCGGGAACGTGTTCCAGTTTCTTTTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGAACCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTccc                                                                                   <  1:1328902/149‑1 (MQ=255)

                                                         gCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGAACCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGt                                                                                 >  1:280425/1‑149 (MQ=255)

                                                          cGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGAACCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACtgttgt                                                                                      >  2:1596682/1‑143 (MQ=255)

                                                                 aaCGTGTTCCAGTTTCTATTTATTTCGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGAACCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCg                                                                         >  1:1071448/1‑149 (MQ=255)

                                                                   cGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGAACCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGt                                                                       <  1:1564768/149‑1 (MQ=255)

                                                                   cGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGAACCTGTTGAAAAACACGGTCAGCCAGATGGCTTACAAGCCCGCGATTTCTACTGTTGTCCCGTCCCCCCCGGt                                                                       >  1:521263/1‑149 (MQ=255)

                                                                   cGTGTTCCAGTTACAATTTAATTGGTAAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTGTTGATCAGTTCGTGAACCTGTTGAAAAACACGGTCAGCCAGATGGTTGACAAGCACGCGATTTCTACTGTTGTCCCGTCTCg                                                                             >  2:423073/1‑143 (MQ=255)

                                                                    gtgtTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGAACCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTACCGACTCACCCGGtt                                                                      >  1:1763389/1‑149 (MQ=255)

                                                                     tgtTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGAACCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGttt                                                                     <  1:481713/149‑1 (MQ=255)

                                                                      gtTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGAACCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTtc                                                                    <  1:489815/149‑1 (MQ=255)

                                                                      gtTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGAACCTGTTGAAAAACACGGACAGCCAGATGGTTTACAAGCACGCGAATTCTACGATTGTCCAGTACCGccc                                                                          >  2:418379/1‑143 (MQ=255)

                                                                      gtTCCAGTTTCTATGTATTTGGTGAATGGTAGTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGAACCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTtc                                                                    <  1:1589199/149‑1 (MQ=255)

                                                                       ttCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGAACCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCACGTCTCGccag                                                                         >  2:933297/1‑141 (MQ=255)

                                                                         ccAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGAACCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGt                                                                       <  2:224325/143‑1 (MQ=255)

                                                                              ttCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGAACCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCTCATcaca                                                            >  1:285152/1‑149 (MQ=255)

                                                                               tCTATTTCGTTGGTGAATGGCATTAAGCTGCAAGGGCAAATCGAGCCATTTGAGCAGTTCGTGAACCTGTTGAAAAACACGGTCTGCCAGATGGTTTACTAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCGCATCACAg                                                           <  1:598567/149‑1 (MQ=255)

                                                                                 tatttagtTGGTGAATGGTATTAACCTGCAAGGGCAAATCGAGTCTTGTGATCAGTTCGTGAACCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGAGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCTCATCACAGTa                                                         <  1:382567/149‑1 (MQ=255)

                                                                                  atttatttGGTGAATGGTATTAAGCTGCAAGTGCAAATCGAGTCTTTTGATCAGTTCGTGAACCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCTCATCACAGTaa                                                        >  1:465486/1‑149 (MQ=255)

                                                                                     tatttGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTGGTGAACCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCTCATCACAGTaacaa                                                     >  1:1013704/1‑149 (MQ=255)

                                                                                        ttGGTGAATGGTATTAAGCTGCAGGGGCAAATCGAGTCTTTTGATCAGTTCGTGAACCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCTCATCACAGTAACAACGc                                                  >  1:1249302/1‑149 (MQ=255)

                                                                                        ttGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGAACCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTGTCATCACAGGaa                                                        >  2:1585384/1‑143 (MQ=255)

                                                                                         tGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGAACCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCTCATCACAGTAACAACGcc                                                 >  1:699193/1‑149 (MQ=255)

                                                                                          ggTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGGGAACCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCTCATCACAGTAACAACGCCg                                                <  1:704849/149‑1 (MQ=255)

                                                                                           gTGATTGGTATTAAGCTGCAAGGGCAAATCGGGTCTTTTGCTCAGTTCGTGAACCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCTCATCACAGTaacat                                                     <  2:807736/143‑2 (MQ=255)

                                                                                           gTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGAACCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTATACTGTTGTCCCGTCTCGCCCGGTTTCTCATCACAGTaacaa                                                     >  2:399871/1‑143 (MQ=255)

                                                                                            tGAATGGTATTAAGCTGCACGGGCAAATCGATGCTGTTGATCAGTTCGTGAACCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGAACGCAATTTCTACTGTTGTCCCGTCTGGCCCGGTTTCTCATCACAGTaacaac                                                    >  2:473549/1‑143 (MQ=255)

                                                                                              aaTGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGAACCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCTCATCACAGTAACAACGCCGGTgg                                            <  1:469486/149‑1 (MQ=255)

                                                                                              aaTGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGAACCTGTAGTAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCTCATCACAGTAACAACGCCGGTgg                                            >  1:1313766/1‑149 (MQ=255)

                                                                                              aaTGGTATTAAGCTGCAAGGGCAAATCGAGTCCTTTGATCAGTTCGTGAACCTGTTGAAAAACAAGCTTTTCCAGAGGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCTCCCGGTTTCTCATACCAGTAACAACGCCGGTgg                                            <  1:1397978/149‑1 (MQ=255)

                                                                                              aaTGGTATTAAGCTGCAAGGGCAAAGCGTGTCTTTTGATCAGTTCGTAAACCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCTCATCACAGTAACAACGCCGGTgg                                            >  1:1071197/1‑149 (MQ=255)

                                                                                                tGGTATTCAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGAACCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCTCATCACAGTCACAACGCCg                                                <  2:17962/143‑1 (MQ=255)

                                                                                                   tATTAAGCTGCAAGGGCAAATCGGGTCTTTTGATCAGTTCGTGAACCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCTCATCACAGTAACAACGCCGGTg                                             <  2:1135058/143‑1 (MQ=255)

                                                                                                   tATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGAACCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCGCGCCCGGTTTCTCATGCCAGTAACAACGCCGGTGGCGGTa                                       >  1:1510460/1‑149 (MQ=255)

                                                                                                    aTTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGAACCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCTCATCACAGTAACAACGCCGGTgg                                            <  2:906519/143‑1 (MQ=255)

                                                                                                    aTTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGAACCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCTCATCACAGTAACAACGCCGGTGGCGGTAc                                      <  1:1618864/149‑1 (MQ=255)

                                                                                                       aaGCTGCAAGGGCAAATCGCGTCTTTTGATCAGTTCGTGAACCTGTTGAAAATCACGGTCAGCCAGATGGTTTACAAGCAGGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCTCATCACAGTAACAACGCCGGTGGCGGTACcag                                   <  1:1412628/149‑1 (MQ=255)

                                                                                                        aGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGAACCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCTCATCACAGTAACAACGCCGGTGGCGGAACcagc                                  >  1:1326874/1‑149 (MQ=255)

                                                                                                           tGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGAACCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCTCATCACAGTAACAACGCCGGTGGCGGTACCAGCAGt                               <  1:298812/149‑1 (MQ=255)

                                                                                                             cAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGAACCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCTCATCACAGTAACAACGCCGGTGGCGGGACCAGCAGTaa                             >  1:52381/1‑149 (MQ=255)

                                                                                                             cAAGGGCAAAGCGAGGCTTTTGATCAGTTCGTGAACCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCTCATCACAGTAACAACGCCGGTGGCGGTACCAGCAGTaa                             <  1:715457/149‑1 (MQ=255)

                                                                                                              taGGGCAAATCGAGTCTTTTGATCAGTTCGTGAACCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCTCATCACAGTAACAACGCCGGTGGCGGTACCAGCAGTAAc                            <  1:1499904/148‑1 (MQ=255)

                                                                                                               aGGGCAAATCGAGTCTTTTGATCAGTTCGTGAACCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCTCATCACAGTAACAACGCCGGTGGCGGTACCAGCAGTAACt                           <  1:1459692/149‑1 (MQ=255)

                                                                                                                gggCAAATCGAGTCTTTTGATCAGTTCGTGAACCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTGGCCCGGTTTCTCATCACAGTAACAAAGCCGGTGGCGGTACCAGCAGTGACtt                          >  1:519706/1‑148 (MQ=255)

                                                                                                                gggCAAATCGAGTCTTTTGATCAGTTCGTGAACCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCTCATCACAGTAACAACGCCGGTGGCGGTACCAGCAGTAACTa                          <  1:1345045/149‑1 (MQ=255)

                                                                                                                gggCAAATCGAGTCTTTTGATCAGTTCGTGAACCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCTCATCACAGTAACAACGCCGGTGGCGGTACCAGCAGTAACTa                          <  1:1708323/149‑1 (MQ=255)

                                                                                                                 ggCAAATCGAGTCTTTTGATCAGTTCGTGAACCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCTCATCACAGTAACAACGCCGGTGGCGGTACCAGCAGTAACTAc                         >  1:100591/1‑149 (MQ=255)

                                                                                                                 ggCAAATCGAGTCTTTTGATCAGTTCGTGAACCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCTCATCACAGTAACAACGCCGGTGGCGGTACCAGCAGTAACTAc                         <  1:893125/149‑1 (MQ=255)

                                                                                                                     aaTCGAGTCTTTTGATCAGTTCGTGAACCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCTCATCACAGTAACAACGCCGGTGGCGGTACCCGCCGTAACt                           >  2:1753972/1‑143 (MQ=255)

                                                                                                                      aTCGAGTCTTTTGATCAGTTCGTGAACCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCTCATCACAGTAACAACGCCGGTGGCGGTACCAGCAGTAACTa                          >  2:561934/1‑143 (MQ=255)

                                                                                                                        ggcgaccattGATCAGTTCGTGAACCGGTTGTAAAACACGGGCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCTCATCACAGTAACAACGCCGGTGGCGGTACCAGCAGTAACTAcc                        <  2:1205097/135‑1 (MQ=255)

                                                                                                                        cGAGTCTTTTGATCAGTTCGTGAACCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCTCATCACAGTAACAACGCCGGTGGCGGTACCAGCAGTAACTACCATCATg                  <  1:92090/149‑1 (MQ=255)

                                                                                                                          aGTCTTTTGATCAGATCGTGAACCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCAAGCGATTTCTACTGTTGTACCGTCTCGCCCGGATTCTCATCACAGTAACAACGCCGGTGGCGGTACcagcatgtactaaccg                      >  2:211490/1‑132 (MQ=255)

                                                                                                                             cTTGTGATCAGTTCGTGAACCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCTCATCACAGTAACAACGCCGGTGGCGGTACCAGCAGTAACTACCATCATGGTagc             <  1:1024372/149‑1 (MQ=255)

                                                                                                                               tttGATCAGTTCGTGAACCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCTCATCACAGTAACAACGCCGGTGGCGGTACCAGCAGTAACTACCATCATGGTagcag           >  1:440039/1‑149 (MQ=255)

                                                                                                                                 tGATCAGTCCGTCAACCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCTCATCACAGTAACAACGCCGGTGGCGGTACCAGCAGTAACTACCATCATGGTa               <  2:1592814/143‑1 (MQ=255)

                                                                                                                                   aTCAGTTCGTGAACCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCTCATCACAGTAACAACGCCGGTGGCGGTACCAGCAGTAACTACCATCATGGTagc             >  2:506731/1‑143 (MQ=255)

                                                                                                                                    tCAGTTCGTGAACCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCTCATCACAGTAACAACGCCGGTGGCGGTACCAGCAGTAACTACCATCATGGTagca            >  2:1517320/1‑143 (MQ=255)

                                                                                                                                       gTTCGTGAACCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCTCATCACAGTAACAACGCCGGTGGCGGTACCAGCAGTAACTACCATCGTGGTAGCAGCGCGCCGa   >  1:136900/1‑149 (MQ=255)

                                                                                                                                       gTTCGTGAACCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCTCATCACAGTAACAACGCCGGTGGCGGTACCAGCAGTAACTACCATCATGGTAGCAGCGCGCAGa   >  1:1768048/1‑149 (MQ=255)

                                                                                                                                        ttCGTGAACCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCTCATCACAGTAACAACGCCGGTGGCGGTACCAGCAGTAACTGCCGTGTTGGTGGGAGCGCGCCGaa  >  1:297742/1‑149 (MQ=255)

                                                                                                                                        ttCGTGAACCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCTCATCACAGTAACAACGCCGGTGGCGGTACCAGCAGTAACTACCAGCATGGTAGCAGCGCGCAGaa  >  1:1247110/1‑149 (MQ=255)

AGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCTCATCACAGTAACAACGCCGGTGGCGGTACCAGCAGTAACTACCATCATGGTAGCAGCGCGCAGAA  >  CP009273/4390092‑4390376

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`