Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,208,0070TG22.3% 228.5 / 18.2 112S534S (TCT→TCG) rpoDRNA polymerase, sigma 70 (sigma D) factor
Reads supporting (aligned to +/- strand):  ref base T (46/41);  new base G (0/25);  total (46/66)
Fisher's exact test for biased strand distribution p-value = 2.36e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 113 total aligned reads displayed.

CTGATGCCGGAAGACAAGATCCGCAAAGTGCTGAAGATCGCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTGGGTAAACAGTTCGACGTTACCC  >  CP009273/3207867‑3208155
                                                                                                                                            |                                                                                                                                                    
cTGATGCCGGAAGACAAGATCCGCAAAGTGCTGAAGATCGCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCTAGCTGCCGCTGGATTCTGCGaccac                                                                                                                                              <  1:1073545/149‑1 (MQ=255)
cTGATGCCGGAAGACAAGATCCGCAAAGTGCTGAAGATCGCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGaccac                                                                                                                                              <  1:279108/149‑1 (MQ=255)
 tGATGCCGGAAGACAAGATCCGCAAGGTGCTGAAGATCGCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGTGTGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCg                                                                                                                                                   <  2:7482/143‑1 (MQ=255)
    tGCCGGAAGACAAGATCCGCAAAGTGCTGAAGATCGCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGGTGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGaa                                                                                                                                          >  1:19445/1‑149 (MQ=255)
      ccGGAAGACAAGATCCGCAAAGTGCTGAAGATCGCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATGCTGCGaccac                                                                                                                                              >  2:1652501/1‑143 (MQ=255)
       cGGAAGACAAGATCCGCAAAGTGCTGAAGATCGCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGc                                                                                                                                       >  1:806871/1‑149 (MQ=255)
       cGGAAGACAAGATCCGCAAAGTGCTGAAGATCGCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGAGaccacc                                                                                                                                             >  2:1454805/1‑143 (MQ=255)
            gACAAGATCCGCAAAGTGCTGAAGATCGCGAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGCAGATTCGCATCTGGGGGGTTTCAGCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGCCCCCCGAAAg                                                                                                                                        >  2:1657149/1‑143 (MQ=255)
               aaGATCCGCAAAGTGCTGAAGATCGCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCtgcgtgc                                                                                                                               <  1:978680/149‑1 (MQ=255)
               aaGATCCGCAAAGTGCTGAAGATCGCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCtgcgtgc                                                                                                                               <  1:1424107/149‑1 (MQ=255)
                 gATCCGCAAAGTGCTGAAGATCGCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCgg                                                                                                                             <  1:1105937/149‑1 (MQ=255)
                 gATCCGCAAAGTGCTGAAGATCGCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTATGCGCCCACCGGAAGCCTGCGTGCgg                                                                                                                             >  1:507467/1‑149 (MQ=255)
                 caTCCGCAAAGTGCTGAAGATCGCCAAAGAGCCAATCTCCAAGGAAACGCCGAACAGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACGCCCCTCGAGCTGCCTCTGGATGCTGCGACCACCGAAAGACtgg                                                                                                                                   >  2:233495/2‑142 (MQ=255)
                  aTCCGCAAAGTGCTGAAGATCGCCAAAGAGCCAATCTCCACGGAAACGCCGATCGGTGATGATGAAGATTCGCACCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGc                                                                                                                            <  1:1518657/149‑1 (MQ=255)
                   tCCGCAAAGTGCTGAAGATCGCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCAACCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCtgcgc                                                                                                                                 >  2:1093536/1‑142 (MQ=255)
                       cAAAGGGCTGAAGATCGGCAAAGAGCCAACCTCCATGGAACCGGCGATCGGTGATGATGAAGATTCGCATCTGGAGGATTTCATCGCGGATACCACCCTCGTGCTGTTGAATGATACGGCGACCACCGAGAGCCTGCGTGCgg                                                                                                                             <  2:1143982/143‑1 (MQ=255)
                          aGTGCTTAAGATCGCCAAAGCGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCAGCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCacg                                                                                                                    <  1:1786783/149‑1 (MQ=255)
                             gCTGAAGATCGCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAGGCCtgcgtgcgttgccgcacgcag                                                                                                                 <  1:1422608/149‑14 (MQ=255)
                              cTGAATATCGCCAAAGAGCCAATCGCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGt                                                                                                                <  1:1374619/149‑1 (MQ=255)
                                 taGATCGCCTAAGAGGCAATCTCCCTGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGCTACCACCCTTTTTTTTTCTCATGATACGGCGACCACCGAGAGCCTGCGTGCGGCAACGCacga                                                                                                                   <  2:549618/142‑1 (MQ=255)
                                 aaGATCGCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGCTTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTgct                                                                                                             >  1:945401/1‑149 (MQ=255)
                                 aaGATCGCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTgct                                                                                                             >  1:142159/1‑149 (MQ=255)
                                      cGCCAAAGATCCAATCTCCATGGCAACGCAGCTACGTGATGATGAAGCTTCGCATCTGGGGGATTTCATCGAGCATACCAGCCTCGTGTTGTCGCAGGATTCGGCGACCACCGAGAGCCTGCGTGCGGCAACGCACGACGTgc                                                                                                              <  2:369428/143‑1 (MQ=255)
                                        ccAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGGgccg                                                                                                            >  2:1572425/1‑141 (MQ=255)
                                          aaaGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCt                                                                                                    >  1:1652410/1‑149 (MQ=255)
                                              ggCCAATCTCCATCGAAACGCCGATCGGGGATGAGTAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTTTTTTTTTTTAAGGATACGGCGACCACCGAGAGCCTGCGTGCGGCAACGCACGACGTGCtggctggc                                                                                                      <  2:503557/142‑1 (MQ=255)
                                               gCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCCACGCCCGACGGCctggccgggc                                                                                                     >  2:1428658/1‑141 (MQ=255)
                                                ccAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCCCGCCGTGCTGCCTGGccc                                                                                                    >  2:869076/1‑142 (MQ=255)
                                                 cAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCGGCGAGCGGCGACGGACGGCGTgctggtcgggcgt                                                                                                   >  2:1614729/1‑135 (MQ=255)
                                                  agtctcCGTGGAAACGCCGATCGGTGATGATGTAGATTCGCATCTGGGGGATTTCATCGAGGATATCACCCTTTTGTTTTTTAAGGATTCGGCGACCACCGAGAGCCTGCGTGCGGCAACGCACGACGTGCGGGCTGGCCTGa                                                                                                  <  2:1525304/141‑1 (MQ=255)
                                                  aaTCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCATGACGTGCTGGCTGGCCTGACcgcgc                                                                                            >  1:1158427/1‑149 (MQ=255)
                                                  aaTCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACcgcgc                                                                                            <  1:306558/149‑1 (MQ=255)
                                                  aaTCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTCCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACcgcgc                                                                                            <  1:1642747/149‑1 (MQ=255)
                                                    tctcCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGt                                                                                          <  1:862473/149‑1 (MQ=255)
                                                       ccATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGCGCGGGCCGGCCTGCCcgcg                                                                                             >  2:1369140/1‑143 (MQ=255)
                                                       ccAAGGAACCGCCGATCGGGGATGATGAAGATTCGCATCTGGCGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCATCGAAAGCCTGCGTGCGGCAACGCACCACGTGCTGGCTGGCCTGACCGCGCGTGaa                                                                                       <  1:1345368/149‑1 (MQ=255)
                                                        cctGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTAGGGGATTTCCGCGGGGATACCACCCGCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCCACGAACGACGTGCTGGCAGGCCCGTTCGCGtgtggtg                                                                                      >  1:1602880/3‑146 (MQ=255)
                                                         atggctgggCCGGTCGGTGTTGGTGACGATTCGCCTCTGGGGGATTGCATCGAGGTTACCACCCTTTGTTTGTCTCTTGATCCGGCGACCACCGAGAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACcgcgcg                                                                                           <  2:842982/135‑1 (MQ=255)
                                                           ggAAGCGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGAGTTCATCGAGGATACCACCCTCGAGCTGCCGATTGATACGGCGACCACCGAGAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTg                                                                                         <  2:1465683/143‑1 (MQ=255)
                                                            gAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGCGGATACCACCCTAGGGCGGCCGCTGGATTCTGCGAACACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGGCCGCGAGTGAAGCaaa                                                                                  >  1:361501/1‑149 (MQ=255)
                                                               aCGCCGATCGGTGATGATGATGATTCGCATCTGGGGGATTTCATCGGGGATACCACCCTCGTGCTGTCGCATGATTCGGCGACCACCGAGAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGc                                                                                     <  2:118535/143‑1 (MQ=255)
                                                                 gCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTc                                                                             >  1:942936/1‑149 (MQ=255)
                                                                  ccGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCt                                                                            <  1:252609/149‑1 (MQ=255)
                                                                    catgggTGAGGATGAAGATTCTGATCTGTGGTGTTTCCTCGAGGATACCACCCTTTTTTTTTTTAAGGATACGGCGACCACCGAGAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAg                                                                                <  2:1369183/139‑1 (MQ=255)
                                                                        ggggatgttgaAGATTCGCATCGGGGGGATTTCATCGAGGATACCACCCTCGTGTTGTCGATTGATTCGGCGACCACCGAGAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCt                                                                            <  2:1008673/140‑1 (MQ=255)
                                                                        ggggatgatgtGGCTGCGCTTCTGGGGGAGTTCATCGAGGATACCACTCTTGTGTTTTCGCATGATTCGGCGACCACCGAGAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCt                                                                            <  2:506025/140‑1 (MQ=255)
                                                                        ggTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCTTGTTTTCGCATGATACGGCGACCACCGAGAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCt                                                                            <  2:1087523/143‑1 (MQ=255)
                                                                          tggtgttgaAGTTTCGTATCTGGGGGATTTCATCGAGGTTGCCGCTTTTGTGTTGTGTGTGGATGCGGCGACCACCGAGAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGc                                                                          <  2:1040701/137‑1 (MQ=255)
                                                                          tgatgatgaAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGc                                                                    >  1:1454222/1‑149 (MQ=255)
                                                                              gaggaAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTCAAGCAAAAGTTCTGCGTATGCGttt                                                                <  1:960734/146‑1 (MQ=255)
                                                                               atgaAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTc                                                               >  1:859398/1‑149 (MQ=255)
                                                                                tgaATATTCGCATCTGGTGGAGTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCg                                                              <  1:723989/149‑1 (MQ=255)
                                                                                tgaAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGCAGTGCTGGCTGGGCTGACCGCGCGTGCGGCCAAAGAGCTGCGCATGc                                                                    >  2:442846/1‑143 (MQ=255)
                                                                                cgcaGATCCGCATCTGGCTGATTTCATCGAGGATACCCCCCTCGAGCCGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCg                                                              <  1:966689/146‑1 (MQ=255)
                                                                                 tgagtctCGCATGTTGGGGATTTCATCGAGGATACCCCCCTGGTGTTGCTGCAGGATACGGCGACCACCGAGAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCg                                                                   <  2:1551299/137‑1 (MQ=255)
                                                                                  aaGATTCGCATCTGGGGGATTTCATGGAGGATACCACCTTCGTTTTGTTTCTGGATTCGGCGACCACCGAGAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGt                                                                  <  2:1569411/143‑1 (MQ=255)
                                                                                  aaGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGGGAGGCAAAAGTTCTGCGTCTGCGTTTCggg                                                            >  1:33780/1‑148 (MQ=255)
                                                                                     gttCGCATCTGCGGTATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATc                                                         <  1:13083/148‑1 (MQ=255)
                                                                                      ttCGCATCCGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCg                                                        <  1:195936/149‑1 (MQ=255)
                                                                                        cGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGat                                                      <  1:1573496/149‑1 (MQ=255)
                                                                                         gCATCTGGGGCATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGGGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGata                                                     <  1:1852488/149‑1 (MQ=255)
                                                                                          cagcTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGatat                                                    <  1:854714/146‑1 (MQ=255)
                                                                                           aTCTGGGGGATTTCATCGAGGATACCACCGTCGTTCTTCTGATGGATTCGGCGACCACCGAGAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATc                                                         <  2:33827/143‑1 (MQ=255)
                                                                                             cTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGaa                                                 <  1:1676619/149‑1 (MQ=255)
                                                                                             cTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGaa                                                 <  1:1698376/149‑1 (MQ=255)
                                                                                              tGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACGACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGGCCGCGGGTGAAGCAACAGTTCTGCGGATGCGTTTCgggcgcaat                                                      >  2:615580/1‑136 (MQ=255)
                                                                                              tGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGGATCGATATGacc                                                >  1:845266/1‑147 (MQ=255)
                                                                                                 gggATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACAcc                                             <  1:1037410/149‑1 (MQ=255)
                                                                                                 gggATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACAcc                                             >  1:8168/1‑149 (MQ=255)
                                                                                                  ggATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGCAAGCCTGCGTGCGGCAACACCCGACGTGCTGGCTGGCCTGACCGCGTTAATTTTAAATTTTCTGCGTATGCGTTTCGGTATCGATCTGAACACCg                                            >  1:626321/1‑149 (MQ=255)
                                                                                                      ttCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACCACGACtc                                        >  1:656306/1‑148 (MQ=255)
                                                                                                      ttCAGCGAGGATACCACCCCCGAGCTGCCGCTGGATTCGGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTa                                        <  1:211674/149‑1 (MQ=255)
                                                                                                      ttCACCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTa                                        <  1:564658/149‑1 (MQ=255)
                                                                                                         aTCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTacac                                     >  1:1443206/1‑149 (MQ=255)
                                                                                                         aTCGAGGATACCACCCTCGAGCTGCCGCTCGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCAGTATCGATATGAACACCGACTacac                                     >  1:1162662/1‑149 (MQ=255)
                                                                                                          tCGAGGATCCCACCCTCGGGCTGTCGCTGGATTCTGCGACCACCGAGAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGAc                                          <  2:1709924/143‑1 (MQ=255)
                                                                                                          tCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAACCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAAAACCGACTACACg                                    >  1:1068268/1‑149 (MQ=255)
                                                                                                           gcaGGATACATCCCTTGATTTGCTGAAGGATTCGGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACt                                         <  2:1326850/141‑1 (MQ=255)
                                                                                                            gAGGTTCCCCCCCTCGAGCTGCCGCTGTATTCTGCGGCCAGCTAAAGCGTGCGTGCGGCAACGAACGACGGGCTGGCTGGCCTGCCCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCt                                  <  1:527060/149‑1 (MQ=255)
                                                                                                             gggATACCACCTTCGAGTTGTCGCTGGATTCGGCGACCACCGAGAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTac                                       <  2:1353736/142‑1 (MQ=255)
                                                                                                             aGGATACCACCCTCGAGCTGCCGCTGGATTCGGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTg                                 <  1:1707026/149‑1 (MQ=255)
                                                                                                              ggATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTgg                                >  1:1133380/1‑149 (MQ=255)
                                                                                                                  cccaccCTTGTTCTGTTGCAGGATTCGGCGACCACCGAGAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCt                                  <  2:211210/142‑1 (MQ=255)
                                                                                                                  accaccATCGAGCGGCCGCGGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGgaaga                            <  1:149776/149‑1 (MQ=255)
                                                                                                                     accCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGt                         <  1:1780291/149‑1 (MQ=255)
                                                                                                                      cccTCGAGCTGCCGCTGGATTCTGCGACCCCCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTg                        <  1:209478/149‑1 (MQ=255)
                                                                                                                      cccTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAAAACCGACTACACGGTGGAAGAAGTg                        >  1:1076507/1‑149 (MQ=255)
                                                                                                                       ccTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTgg                       <  1:1706721/149‑1 (MQ=255)
                                                                                                                        cTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTggg                      >  1:404239/1‑149 (MQ=255)
                                                                                                                           gtgCTTCCGCTGGATTCTGCGACCACCGAGAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGt                         <  2:1340739/141‑1 (MQ=255)
                                                                                                                            agttGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTg                        <  2:129879/140‑1 (MQ=255)
                                                                                                                             tttGCCGGCGGATACGGGGACCGCCGAGAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTgg                       <  2:1360127/141‑1 (MQ=255)
                                                                                                                                 ccGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGAAAAAGTTCTGAGTCTACGTTTCGGTCTAGCTATAAGCACCGACTGGATGCTAGTAGAAATGGGtgc                   >  2:1206430/1‑141 (MQ=255)
                                                                                                                                   gCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGACAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTGGGTAAACAGCTCg           >  1:248105/1‑149 (MQ=255)
                                                                                                                                        aTTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTAGCTGACCTGACCGCGCGTGAAGCAAAGGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGCAGAAGTGGGTAAACAGTTc            >  2:627419/1‑143 (MQ=255)
                                                                                                                                         ttCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACATGCTGGCTGGCCCGATCGCGCGTGAAGCAAAAGTTCTGCGTCTGCGTTTCGGTATCGATATGAACACCGCCTACACGCTGGAAGAAGTGGGTAAACCGTTCg           >  2:349787/1‑143 (MQ=255)
                                                                                                                                         ttCTGCGAACACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCAACTCACTATCGATATGAACACCGACTACACGCTGGAAGAAGTGGGTAAACAGTTCGACGTTa     >  1:1390579/1‑149 (MQ=255)
                                                                                                                                           cTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTGGGTAAACAGTTCGAc         <  2:852629/143‑1 (MQ=255)
                                                                                                                                           cTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTGGGTAAACAGTTCGACGTTAcc   >  1:1302392/1‑149 (MQ=255)
                                                                                                                                            tGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTGGGTAAACAGTTCGACGGTAccc  >  1:971998/1‑149 (MQ=255)
                                                                                                                                            |                                                                                                                                                    
CTGATGCCGGAAGACAAGATCCGCAAAGTGCTGAAGATCGCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTGGGTAAACAGTTCGACGTTACCC  >  CP009273/3207867‑3208155

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑