BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP009273	2,420,470	C→A	intergenic (‑203/+18)	hisJ ← / ← argT	histidine/lysine/arginine/ornithine transporter subunit/lysine/arginine/ornithine transporter subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	2,420,470	0	C	A	100.0%	96.6 / NA	32	intergenic (‑203/+18)	hisJ/argT	histidine/lysine/arginine/ornithine transporter subunit/lysine/arginine/ornithine transporter subunit
Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (14/18); total (14/18)

TCGTGCCATAAAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCATGATGATCATTCCATCAGGTACAGCTTCCCAGCGACGTATCAGTCACCGTAGACATTAAAGTCGAAATACTTTTTCGCCATCTTGTCGTAGGTGCCGTCCTGACGCAGCTCGCCAAGCGCCTTATTGAAGGCAGCCGTCAGTTCAGCATCATCTTTACGTAGCCCT  >  CP009273/2420324‑2420614

gaaaccccgcagttgcgctggagaaggccagaaccagagagagcgatagcagcagttttttcataatgtcctcaactgacagtctttttatagggaatatttacaggtttgttacacttatcgtgccataaaaATTCCATCAGGTAAAg                                                                                                                                                <  2:330420‑M2/16‑1 (MQ=255)

     gccgcagttgcgctggagaaggccagaaccagagagagcgatagcaccagttttttcataatgtcctccactgacagtccttttatagggaatatttactggtttgttacacttatcgtgccataaaaATTCCATCAGGTAAAGCTTcc                                                                                                                                           <  2:602896‑M2/21‑1 (MQ=255)

       cgcagttgcgctggagaaggccagaaccagagagagcgatagcaccagttttttcataatgtcctcaactgacagtctttttatagggaatatttacaggtttgttacacttatcgtgccataaaaATTCCATCAGGTAAAGCTTCCCa                                                                                                                                         >  1:694389‑M2/127‑149 (MQ=255)

           gttgcgctggagaaggccagaaccagagagagcgatagcaccagttttttcataatgtcctcaactgacagtctttttatagggaatatttacaggtttgttacacttatcgtgccataaaaATTCCATCAGGTAAAGCTTCCCAGCGa                                                                                                                                     <  1:233871‑M2/27‑1 (MQ=255)

              gcgctggagaaggccagaaccagagagagcgatagcaccagttttttcataatgtcctcaactgacagtctttttatagggaatatttacaggtttgttacacttatcgtgccataaaaATTCCATCAGGTAAAGCTTCCCAGCGACGt                                                                                                                                  >  1:626087‑M2/120‑149 (MQ=255)

                       aaggccagaaccagagagagcgatagcaccagttttttcataatgtcctcaactgacagtctttttatagggaatatttacaggtttgttacacttatcgtgccataaaaATTCCATCAGGTAAAGCTTCCCAGCGACGTATCAGTCAc                                                                                                                         <  1:576907‑M2/39‑1 (MQ=255)

                                           cgatagcaccagttttttcataatgtcctcaactgacagtctttttatagggaatatttacaggtttgttacacttatcgtgccataaaaATTCCATCAGGTAAAGCTTCCCAGCGACGTATCAGTCACCGTAGACATTAAAGTCGaaa                                                                                                     >  1:70203‑M2/91‑149 (MQ=255)

                                              tagcaccagttttttcataatgtcctcaactgacagtctttttatagggaatatttacaggtttgttacacttatcgtgccataaaaATTCCATCAGGTAAAGCTTCCCAGCGACGTATCAGTCACCGTAGACATTAAAGTCGAAATAc                                                                                                  <  2:694389‑M2/62‑1 (MQ=255)

                                                 ccccagttttttcataatgtcctcaattgacagtctttttatagggaatatttacaggtttgttacacttatcgtgccataaaaATTCCATCAGGTAAAGCTTCCCAGCGACGTATCAGTCACCGTAGACATTAAAGTCGAAATACttt                                                                                               <  2:632301‑M2/65‑1 (MQ=255)

                                                  accagttttttcataatgtcctcaactgacagtctttttatagggaatatttacaggtttgttacacttatcgtgccataaaaATTCCATCAGGTAAAGCTTCCCAGCGACGTATCAGTCACCGTAGACATTAAAGTCGCAATAAtttt                                                                                              >  2:541467‑M2/84‑149 (MQ=255)

                                                    cagttttttcataatgtcctcaactgacagtctttttatagggaatatttacaggtttgttacacttatcgtgccataaaaATTCCATCAGGTAAAGCTTCCCAGCGACGTATCAGTCACCGTAGACATTAAAGTCGAAATACTTTTTc                                                                                            <  2:694997‑M2/68‑1 (MQ=255)

                                                    cagttttttcataatgtcctcaactgacagtctttttatagggaatatttacaggtttgttacacttatcgtgccataaaaATTCCATCAGGTAAAGCTTCCCAGCGACGTATCAGTCACCGTAGACATTAAAGTCGAAATACTTTTTc                                                                                            >  1:476092‑M2/82‑149 (MQ=255)

                                                           ttcataatgtcctcaactgacagtctttttatagggaatatttacaggtttgttacacttatcgtgccataaaaATTCCATCAGGTAAAGCTTCCCAGCGACGTATCAGTCACCGTAGACATTAAAGTCGAAATACTTTTTCGCCATCt                                                                                     >  1:146045‑M2/75‑149 (MQ=255)

                                                           ttcataatgtcctcaactgacagtctttttatagggaatatttacaggtttgttacacttatcgtgccataaaaATTCCATCAGGTAAAGCTTCCCAGCGACGTATCAGTCACCGTAGACATTAAAGTCGAAATAATTTTTCCCCctct                                                                                     >  1:82801‑M2/75‑149 (MQ=255)

                                                                        caactgacagtctttttatagggaatatttacaggtttgttacacttatcgtgccataaaaATTCCATCAGGTAAAGCTTCCCAGCGACGTATCAGTCACCGTAGACATTAAAGTCGAAATACTTTTTCGCCATCTTGTCGTAGGTGcc                                                                        <  1:428723‑M2/88‑1 (MQ=255)

                                                                             gacggtttttttatagggaatatttccaggtttgttacacttatcgtgccataaaaATTCCATCAGGTAAAGCTTCCCAGCGACGTATCAGTCACCGTAGACATTAAAGTCGAAATACTTTTTCGCCATCTTGTCGTAGGTGCCGTCCt                                                                   <  2:546292‑M2/93‑1 (MQ=255)

                                                                                  tctttttttagggaatttttacaggtttgttacacctttcgtgccataaaaATTCCATTAGGTAAAGCTTCCCCGCGACGTAACAGTCACCGTAGACATTAAAGTCGAAATACTTTTTCGCCATCTTGTCGTAGGTGCCGTCCTGACGc                                                              <  2:225920‑M2/98‑1 (MQ=255)

                                                                                    tttttatagggaatatttacaggtttgttacacttatcgtgccataaaaATTCCATCAGGTAAAGCTTCCCAGCGACGTATCAGTCACCGTAGACATTAAAGTCGAAATACTTTTTCGCCATCTTGTCGTAGGTGCCGTCCTGACGCAg                                                            >  1:369181‑M2/50‑149 (MQ=255)

                                                                                         atagggaatatttacaggtttgttacacttatcgtgccataaaaATTCCATCAGGTAAAGCTTCCCAGCGACGTATCAGTCACCGTAGACATTAAAGTCGAAATACTTTTTCGCCATCTTGCCGTAGGTCCCTTACTGACGCAGCTCGc                                                       >  2:603710‑M2/45‑149 (MQ=255)

                                                                                             ggaatatttacaggtttgttacacttatcgtgccataaaaATTCCATCAGGTAAAGCTTCCCAGCGACGTATCAGTCACCGTAGACATTAAAGTCGAAATACTTTTTCGCCATCTTGTCGTAGGTGCCGTCCTGACGCAGCTCGCCCAg                                                   >  2:463478‑M2/41‑149 (MQ=255)

                                                                                              gaatatttacaggtttgttacacttatcgtgccataaaaATTCCATCAGGTAAAGCTTCCCAGCGACGTATCAGTCACCGTAGACATTAAAGTCGAAATACTTTTTCGCCATCTTGTCGTAGGTGCCGTCCTGACGCAGCTCGCCAAgc                                                  <  2:154252‑M2/110‑1 (MQ=255)

                                                                                               aatatttacaggtttgttacacttatcgtgccataaaaATTCCATCAGGTAAAGCTTCCCAGCGACGTATCAGTCACCGTAGACATTAAAGTCGAAATACTTTTTCGCCATCTTGTCGTAGGTGCCGTCCTGACGCAGCTCGCCAAgcg                                                 >  2:110292‑M2/39‑149 (MQ=255)

                                                                                               aatatttacaggtttgttacacttatcgtgccataaaaATTCCATCAGGTAAAGCTTCCCAGCGACGTATCAGTCACCGTAGACATTAAAGTCGAAATACTTTTTCGCCATCTGTTCGTAGGTGCCGTCCTGACTCAGCTCGCCAAgcg                                                 >  1:316571‑M2/39‑149 (MQ=255)

                                                                                                    gggcggggttgttacacttatcgtgccataaaaATTCCATCAGGTAAAGCTTCCCAGCGACGTATCAGTCACCGTAGACATTAAAGTCGAAATACTTTTTCGCCATCTTGTCGTAGGTGCCGTCCTGACGCAGCTCGCCAAGCGCCTTa                                            <  2:517337‑M2/116‑1 (MQ=255)

                                                                                                       caggtttgttacacttatcgtgccataaaaAGTCCATCAGGTAAAGCTTCCCAGCGACGTATCAGTCACCGTAGACATTAAAGTCGAAATACTTTTTCGCCATCTTGTCGTAGGTGCCGTCCTGACGCAGCTCGCCAAGCGCCTTATTg                                         <  1:138878‑M2/119‑1 (MQ=255)

                                                                                                               ttacacttatcgtgccataaaaATTCCATCAGGTAAAGCTTCCCAGCGACGTATCAGTCACCGTAGACATTAAATTCGAAATACTTTTTCGCCATCTTGTCGTAGGTGCCGTCCTGACGCAGCTCGCCAAGCGCCTTATTGAAGGCAGc                                 >  1:201995‑M2/23‑149 (MQ=255)

                                                                                                                        tcgtgccataaaaATTCCATCAGGTAAAGCTTCCCAGCGACGTATCAGTCACCGTAGACATTAAAGTCGAAATACTTTTTCGCCATCTTGTCGTAGGTGCCGTCCTGACGCAGCTCGCCAAGCGCCTTATTGAAGGCAGCCGTCAGTTc                        <  1:39986‑M2/136‑1 (MQ=255)

                                                                                                                          gtgccataaaaATTCCATCAGGTAAAGCTTCCCAGCGACGTATCAGTCACCGTAGACATTAAAGTCGAAATACTTTTTCGCCATCTTGTCGTAGGTGCCGTCCTGACGCAGCTCGCCAAGCGCCTTATTGAAGGCAGCCGTCAGTTCAg                      <  2:476092‑M2/138‑1 (MQ=255)

                                                                                                                              cataaaaATTCCATCAGGTAAAGCTTCCAAGCGACGTATCAGTCACCGTAGACATTAAAGTCGAAATACTTTTTCGCCATCTTGTCGTAGGTGCCGTCCTGACGCAGCTCGCCAAGCGCCTTATTGAAGGCAGCCATCAGTTCagcagc                  <  2:602037‑M2/142‑3 (MQ=255)

                                                                                                                                 aaaaATTCCATCAGGTAAAGCTTCCCAGCGACGTATCAGTCACCGTAGACATTAAAGTCGAAATACTTTTTCGCCATCTTGTCGTAGGTGCCGTCCTGACGCAGCTCGCCAAGCGCCTTATTGAAGGCAGCCGTCAGTTCAGCatcatc               <  1:88003‑M2/145‑1 (MQ=255)

                                                                                                                                   aaATTCCGTCTGGTAAAGCTGCCCAGCGACGTATCAGTCACCGTAGACATTAAAGTCGAAATACTTTTTCGCCATCTTGTCGTAGGTGCCGTCCTGACGCAGCTCGCCAAGCGCCTTATTGAAGGCAGCCGTCAGTTCAGCATCATCtt             <  2:53794‑M2/147‑1 (MQ=255)

                                                                                                                                              ggTAAAGCTTCCCAGCGACGTATCAGTCACCGTAGACATTAAAGTCGAAATACTTTTTCGCCATCTTGTCGTAGGTGCCGTCCTGACGCAGCTCGCCAAGCGCCTTATTGAAGGCAGCCGTCAGTTCAGCATCATCTTTACGTAGCCCt  >  1:373800/1‑149 (MQ=255)

TCGTGCCATAAAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCATGATGATCATTCCATCAGGTACAGCTTCCCAGCGACGTATCAGTCACCGTAGACATTAAAGTCGAAATACTTTTTCGCCATCTTGTCGTAGGTGCCGTCCTGACGCAGCTCGCCAAGCGCCTTATTGAAGGCAGCCGTCAGTTCAGCATCATCTTTACGTAGCCCT  >  CP009273/2420324‑2420614

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`