| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 2,725,094 | A→G | 21.0% | G813G (GGT→GGC) | clpB ← | protein disaggregation chaperone |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 2,725,094 | 0 | A | G | 21.0% | 30.3 / 2.8 | 19 | G813G (GGT→GGC) | clpB | protein disaggregation chaperone |
| Reads supporting (aligned to +/- strand): ref base A (6/9); new base G (2/2); total (8/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.72e-01 | |||||||||||
TCATTTACTGGACGGCGACAATCCGGTCTTCATTAACTTCCAGGCGAATCACTTTACCCGGAACCAATTCACCAGACAGTATTTGCTGTGCCAGCGGGTTTTCGATCTGCTGCTGAATTGCACGTTTCAGAGGACGTGCACCATAGACCGGATCGTAACCGTTCTCGCTCAGCAGTTTCAGCGCCTCGTCAGAAATGTGGATTTCATAACCACGTTCTTCCAGACGTTTGTACAGACGTTTCAACTGAATCTGCGCAATCGAGGCAATGTGCTGTT > CP009273/2724955‑2725230 | tCATTTACTGGACGGCGACAATCCGGTCTTCATTAACTTCCAGGCGAATCACTTTACCCGGAACCAATTCACCAGACAGTATTTGCTGTGCCAGCGGGTTTTCGATCTGCTGCTGAATTTCACGTTTCAGAGGACGTGCACCATAGAcg > 2:86280/1‑148 (MQ=255)tCATTTACTGGACGGCGACAATCCGGTCTTCATTAACTTCCAGGCGAATCACTTTACCCGGAACCAATTCACCAGACAGTATTTGCTGTGCCAGCGGGTTTTCGATCTGCTGCTGAATTGCACGTTTCAGAGGACGTGCACCATAGAcc > 2:157271/1‑149 (MQ=255) ggACGGCGACAATCCGGTCTTCATTAACTTCCAGGCGAATCACTTTACCCGGAACCAATTCACCAGACAGTATTTGCTGTGCCAGCGGGTTTTCGATCTGCTGCTGAATTGCCCGTTTCAGAGGACGTGCGCCGTAGACCGGATCGTaa > 2:36383/1‑149 (MQ=255) ggACGGCGACAATCCGGTCTTCATTAACTTCCAGGCGAATCACTTTACCCGGAACCAATTCACCAGACAGTATTTGCTGTGCCAGCGGGTTTTCGATCTGCTGCTGAATTGCACGTTTCAGAGGACGTGCGCCATAGACCGGATCGTaa > 2:36428/1‑149 (MQ=255) ggcGGCGACAATCCGGTCTTCATTAACTTCCAGGCGAATCACTTTACCCGGAACCAATTCACCAGACAGTATTTGCTGTGCCAGCGGGTTTTCGATCTGCTGCTGAATTGCACGTTTCAGAGGACGTGCACCATAGACCGGATCGTAAc < 1:46330/147‑1 (MQ=255) aTCCGGTCTTCATTAACTTCCAGGCGAATCACTTTACCCGGAACCAATTCACCAGACAGTATTTGCTGTGCCAGCGGGTTTTCGATCTGCTGCTGAATTGCACGTTTCAGAGGACGTGCACCATAGACCGGATCGTAACCGTTCTCGCt < 2:159656/149‑1 (MQ=255) gTCTTCATTAACTTCCAGGCGAATCACTTTACCCGGAACCAATTCACCAGACAGCATTTGCTGTGCCAGCGGGTTTTCGATCTGCTGCTGAATTGTACGTTTCAGAGGACGTGCACCATAGACCGGATCGTAACCGTTCTCGCTcagca < 1:172043/149‑1 (MQ=255) aGGCGAATCACTTTACCCGGAACCAATTCACCAGACAGTATTTGCTGTGCCAGCGGGTTTTCGATCTGCTGCTGAATTGCACGTTTCAGAGGACGTGCACCATAGACCGGATCGTAACCGTTCTCGCTCAGCAGTTTGAGCGCCTCGTc > 2:288470/1‑149 (MQ=255) gCGAATCACTTTACCCGGAACCAATTCACCAGACAGTATTTGCTGTGCCAGCGGGTTTTCGATCTGCTGCTGAATTGCACGTTTCAGAGGACGTGCACCATAGACCGGATCGTAACCGTTCTCGCTCAGCATTTTCCGCGCCTCGTCAg > 2:103261/1‑149 (MQ=255) ttACCCGGAACCAATTCACCAGACAGTATTTGCTGTGCCAGCGGGTTTTCGATCTGCTGCTGCATTGCACGTTTCAGAGGACGTGCACCATAGACCGGATCGTAACCGTTCTCGCTCAGCAGTTTCAGCGCCTCGTCAGAAATGTGGAt < 2:12229/149‑1 (MQ=255) aCCCGGAACCAATTCACCAGACAGTATTTGCTGTGCCAGCGTGTTTTCGATCTGCTGCTGAATTGCACGTTTCAGAGGACGTGCACCATAGACCGGATCGTAACCGTTCTCGCTCAGCAGTTTCAGCGCCTCGTCAGAAATGTGGAttt < 2:199172/149‑1 (MQ=255) ccagccagTATTTGCTGTGCCAGCGGGTTTTCGATCTGCTGCTGAATTGCACGTCTCAGAGGACGTGCACCATAGACCGGCTCGTAACCGTTCTCGCCCAGCAGTTTCCGCGCCTCGTCAGAAATGTGGATTTCATACCCACGttcttc < 1:275741/149‑1 (MQ=255) ctattgtgttggCCGGCTGGATTGCGTTCTGCTGCTGAATTGCACGGTTCAGAGGACGTGCACCATAGACCGGACCGTAACCGTTCTCGCTCAGCAGTTTCAGCGCCTCGTTAGAAATGTGGATTTCAGAACCACGTTCTTCCAGACGt < 1:256163/138‑1 (MQ=255) tGCTGGGCCCGCGGGTGTTCGATCTGCTGCTGAATTGCACGTTTCAGCGGACGTGCACCATAGACCGGATCGTAACCGTTCTCGCTCAGCAGTTTCAGCGCCTCGTCAGAAATGTGGATTTCATAACCACGTTCTTCCAGACGTTTGTa < 1:80506/149‑1 (MQ=255) gggTTTTCGATCTGCTGCTGAATTGCACGTTTCAGAGGACGTGCACCATAGACCGGATCGTAACCGTTCTCGCTCAGCAGTTTCAGCGCCTCGTCAGAAATGTGGATTTCATAACCACGTTCTTCCAGACGTTTGTACAGACGTTTCaa < 2:216101/149‑1 (MQ=255) ttttCGATCTGCTGCTGAATTGCACGTTTCAGAGGACGTGCACCATAGACCGGATCGTAACCGTTCTCGCTCAGCAGTTTCAGCGCCTCGTCAGACATGTGGATTTCATAACCACGTTCCTCCAGACGTTTGTACAGACGTTTCAAcgg > 1:283030/1‑147 (MQ=255) gATCTGCTGCTGAATTGCACGTTTCAGAGGACGTGCACCATAGACCGGATCGTAACCGTTCTCGCGCAGCAGTTTCAGCGCCTCGCCAGAAATGTGGAATTCATTACCACGTTCTTCCAGACGTTTGTAAAGCCGTTCCACCTGactgt > 1:128782/1‑145 (MQ=255) cAGAGGACGTGCGCCATAGACCGGATCGTAACCGTTCTCGCTCAGCAGTTTCAGCGCCTCGTCAGAAATGTGGATTTCATAACCACGTTCTTCCAGACGTTTGTACAGACGTTTCAACTGAATCTGCGCAATCGAGGCAATGTGCTGtt < 1:36428/149‑1 (MQ=255) cAGAGGACGTGCGCCATAGACCGGATCGTAACCGTTCTCGCTCAGCAGTTTCAGCGCCTCGTCAGAAATGTGGATTTCATAACCACGTTCTTCCAGACGTTTGTACAGACGTTTCAACTGAATCTGCGCAATCGAGGCAATGTGCTGtt < 1:36383/149‑1 (MQ=255) | TCATTTACTGGACGGCGACAATCCGGTCTTCATTAACTTCCAGGCGAATCACTTTACCCGGAACCAATTCACCAGACAGTATTTGCTGTGCCAGCGGGTTTTCGATCTGCTGCTGAATTGCACGTTTCAGAGGACGTGCACCATAGACCGGATCGTAACCGTTCTCGCTCAGCAGTTTCAGCGCCTCGTCAGAAATGTGGATTTCATAACCACGTTCTTCCAGACGTTTGTACAGACGTTTCAACTGAATCTGCGCAATCGAGGCAATGTGCTGTT > CP009273/2724955‑2725230 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |