| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 273,525 | T→C | 17.5% | E330G (GAG→GGG) | afuC ← | CP4‑6 prophage; putative ferric transporter subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 273,525 | 0 | T | C | 17.5% | 38.0 / 3.9 | 23 | E330G (GAG→GGG) | afuC | CP4‑6 prophage; putative ferric transporter subunit |
| Reads supporting (aligned to +/- strand): ref base T (10/9); new base C (2/2); total (12/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.17e-01 | |||||||||||
CGCAGCGCATCAGGCATTGCCGGATGGCGGCACAAGCGCCTTATCCGGCCTACAAAACCACAAAACGTTATGCCGCATCCGCCAGAACAAACATGCCGTACGGATGGATTTCAAGATAATACTGCTCGCCGACGTCCGGTTGCAGACGCGTAGCGTTGACCTGCAATAATATCTCCTGCCCGTGCCATTCCACCGTCACTTCATACTGCGGCCCCATATAGGCGACATGGCGGATCACGCAGCGCTGGCTCTCTTCGCCGCGATCGCTG > CP009273/273400‑273668 | cgcagcgcaTCAGGCATTGCCGGATGGCGGCACAAGCGCCTTATCCGGCCTACAAAACCACAAAACGTTATGCCGCATCCGCCAGAACAAACATGCCGTACGGATGGATTTCAAGATAATACTGCTCGCCGACGTCCGGTTGCAGAcgc > 1:86257/1‑149 (MQ=255) tCAGGCATTGCCGGATGGCGGCACAAGCGCCTTATCCGGCCTACAAAACCACAAAACGTTATGCCGCATCCGCCAGAACAAACATGCCGTACGGATGGATTTCAAGATAATACTGCTCGCCGACGTCCGGTTGCAGACGCGTAGCGTTg > 2:101958/1‑149 (MQ=255) cAGGCATTGCCGGATGGCGGCACAAGCGCCTTATCCGGCCTACAAAACCACAAAACGTTATGCCGCATCCGCCAGAACAAACATGCCGTACGGATGGATTTCAACATAATACTGCTCGCCGACGTCCGGTTGCAGACGCGTAGCGTTGa < 1:113233/149‑1 (MQ=255) ggCATTGCCGGATGGCGGCACAAGCGCCTTATCCGGCCTACAAAACCACAAAACGTTATGCCGCATCCGCCAGAACAAACATGCCGTACGGATGGATTTCAAGATAATACTGCTCGCCGACGTCCGGTTGCAGACGCGTAGCGTTGAcc > 2:145597/1‑149 (MQ=255) ttGCCGGATGGCGGCACAAGCGCCTTATCCGGCCTACAAAACCACAAAACGTTATGCCGCATCCGCCAGAACAAACATGCCGTACGGATGGATTTCAAGATAATACTGCTCGCCGACGTCCGGTTGCAGACGCGTAGCGTTGACCTGCa < 2:223397/149‑1 (MQ=255) ttGCCGGATGGCGGCACAAGCGCCTTATCCGGCCTACAAAACCACAAAACGTTATGCCGCATCCGCCAGAACAAACATGCCGTACGGATGGATTTCAAGATAATACTGCTCGCCGACGTCCGGTTGCAGACGCGTAGCGTTGACCTGCa < 2:82040/149‑1 (MQ=255) aTGGCGGCACAAGCGCCTTATCCGGCCTACAAAACCACAAAACGTTATGCCGCATCCGCCAGAACAAACATGCCGTACGGATGGATTTCAAGATAATACTGCTCGCAGACTTCCGGTTGCAGACGCGTAGCGTTGACCTGCAATAatat < 1:81675/149‑1 (MQ=255) gcgcCTTATCCGGCCTACAAAACCACAAAACGTTATGCCGCATCCGCCAGAACAAACATGCCGTACGGATGGATTTCAAGATAATACTGCTCGCCGACGTCCGGTTGCAGACGCGTAGCGTTGACCTGCAATAATATCTCCTGCCCGTg < 2:61904/149‑1 (MQ=255) cgcCTTATCCGGCCTACAAAACCACAAAACGTTATGCCGCATCCGCCAGAACAAACATGCCGTACGGATGGATTTCAAGATAATACTGCCCGCCGACGTCCGGTTGCAGACGCGTAGCGTTGACCTGCAATAATATCTCCTGCCCGTGc > 1:152689/1‑149 (MQ=255) cgcCTTATCCGGCCTACAAAACCACAAAACGTTATGCCGCATCCGCCAGAACAAACATGCCGTACGGATGGATTTCAAGATAATACTGCCCGCCGACGTCCGGTTGCAGACGCGTAGCGTTGACCTGCAATAATATCTCCTGCCCGTGc > 1:152676/1‑149 (MQ=255) caaCAAAACCACAAAACGTTATGCCGCATCCGCCAGAACAAACATGCCGTACGGATGGATTTCAAGATAATACTGCTCGCCGACGTCCGGTTGCAGACGCGTAGCGTTGACCTGCAATAATATCTCCTGCCCGTGCCATTCCACCGTCa < 2:145562/147‑1 (MQ=255) aCAAAACCACAAAACGTTATGCCGCATCCGCCAGAACAAACATGCCGTACGGATGGATTTCAAGATAATACTGCTCGCCGACGTCCGGTTGCAGACGCGTAGCGTTGACCTGCAATAATATCTCCTGCCCGTGCCATTCCACCGTCAcg > 1:173138/1‑148 (MQ=255) cacaAAACGTTATGCCGCATCCGCCAGAACAAACCTGCCGTACGGATGGATTTCAAGGTAATACTGCTCGCCGACGTCCGGTTGCAGACGCGTAGCGTTGGCCTGCCATAATATCGCCTGCCCGTGCCCTTCCACCGTCACTTcctcct > 1:219581/1‑144 (MQ=255) aTCCGCCAGAACAAACATGCCGTACGGATGGATTTCAAGATAATACTGCTCGCCGACGTCCGGTTGCAGACGCGTAGCGTTGACCTGCAATAATATCTCCTGCCCGTGCCATTCCACCGTCACTTCATACTGCGGGCCCATATAGGCGa < 2:220259/149‑1 (MQ=255) aTCCGCCAGAACAAACATGCCGTACGGATGGATTTCAAGATAATACTGCTCGCCGACGTCCGGTTGCAGACGCGTAGCGTTGACCTGCAATAATATCTCCTGCCCGTGCCATTCCACCGTCACTTCATACTGCGGCCCCATATAGGCGa > 1:62059/1‑149 (MQ=255) tCCGCCAGAACAAACATGCCGTACGGATGGATTTCAAGATAATACTGCCCGCCGACGTCCGGTTGCAGACGCGTAGCGTTGACCTGCAATAATATCTCCTGCCCGTGCCATTCCACCGTCACTTCATACTGCGGCCCCATATAGGCGAc < 2:152676/149‑1 (MQ=255) tCCGCCAGAACAAACATGCCGTACGGATGGATTTCAAGATAATACTGCCCGCCGACGTCCGGTTGCAGACGCGTAGCGTTGACCTGCAATAATATCTCCTGCCCGTGCCATTCCACCGTCACTTCATACTGCGGCCCCATATAGGCGAc < 2:152689/149‑1 (MQ=255) gCCAGAACAAACATGCCGTACGGACGGATTTCAAGATAATACTGCTCGCCGACGTCCGGTTGCAGACGCGTAGCGTTGACCTGCAATAATATCTCCTGCCCGTGCCATTCCACCGTCACTTCATACTGCGGCCCCATATAGGCGACATg > 2:232093/1‑149 (MQ=255) ccAGAACAAACATGCCGTACGGATGGATTTCAAGATAATACTGCTCGCCGACGTCCGGTTGCAGACGCGTAGCGTTGACCTGCAATAATATCTCCTGCCCGTGCCATTCCACCGTCACTTCATACTGCGGCCCCCTATAGGCGACATgg > 1:282288/1‑149 (MQ=255) ccAGAACAAACATGCCGTACGGATGGATTTCAAGATAATACTGCTCGCCGACGTCCGGTTGCAGACGCGTAGCGTTGACCTGCAATAATATCTCCTGCCCGTGCCATTCCACCGTCACTTCATACTGCGGCCCCATATAGGCGACATgg > 1:282315/1‑149 (MQ=255) cAAGATAATACTGCTCGCCGACGGCCGGTTGCAGACGCGTAGCGTTGACCTGCAATAATATCTCCTGCCCGTGCCATTCCACCGTCACTTCATACTGCGGCCCCATATAGGCGACATGGCGGATCACGCAGCGCTGGCTCTCTTcgccg < 1:101958/149‑1 (MQ=255) aaGATAATACTGCTCGCCGACGTCCGGTTGCAGACGCGTAGCGTTGACCTGCAATAATATCTCCTGCCCGTGCCATTCCACCGTCACTTCATACTGCGGCCCCATATAGGCGACATGGCGGATCACGCAGCGCTGGCTCTCTTcgccgc < 1:153146/149‑1 (MQ=255) aCTGCTCGCCGACGTCCGGTTGCAGACGCGTAGCGTTGACCTGCAATAATATCTCCTGCCCGTGCCATTCCACCGTCACTTCATACTGCGGCCCCATAAGGGCGACAGGTGGGATCAGGCGGCGCTGGCTCTCTTCGCcgcgctgactg > 2:135605/1‑142 (MQ=255) | CGCAGCGCATCAGGCATTGCCGGATGGCGGCACAAGCGCCTTATCCGGCCTACAAAACCACAAAACGTTATGCCGCATCCGCCAGAACAAACATGCCGTACGGATGGATTTCAAGATAATACTGCTCGCCGACGTCCGGTTGCAGACGCGTAGCGTTGACCTGCAATAATATCTCCTGCCCGTGCCATTCCACCGTCACTTCATACTGCGGCCCCATATAGGCGACATGGCGGATCACGCAGCGCTGGCTCTCTTCGCCGCGATCGCTG > CP009273/273400‑273668 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |