Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
JC	CP009273	2,420,337	Δ120 bp	77.4%	intergenic (‑70/+32)	hisJ ← / ← argT	histidine/lysine/arginine/ornithine transporter subunit/lysine/arginine/ornithine transporter subunit

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	CP009273	= 2420336	3 (0.170)	12 (0.680)	12/276	NT	77.4%	intergenic (‑69/+152)	hisJ/argT	histidine/lysine/arginine/ornithine transporter subunit/lysine/arginine/ornithine transporter subunit
	?	CP009273	2420457 =	4 (0.230)					intergenic (‑190/+31)	hisJ/argT	histidine/lysine/arginine/ornithine transporter subunit/lysine/arginine/ornithine transporter subunit

CGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGCGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCATGATGATCATTCCATCAGGTACAGCTTCCCAGCGA  >  CP009273/2420193‑2420483                                                                                                                                                |                                                                                                                                                    cGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGCGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAAattcc                                                                                                                                                <  1:35700‑M1/149‑6 (MQ=255)     atgggagcaaacgcCGCAGTTGCGGTGGGGAAGGCCAGAACCAGGGAGAGCGATGGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAAattccatca                                                                                                                                            <  1:54152‑M1/144‑10 (MQ=255)         cagcaAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGCGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAAattccatcaggta                                                                                                                                        >  1:88334‑M1/1‑136 (MQ=255)                cgccgcAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGCGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAAattccatcaggtaaagcttc                                                                                                                                 <  1:105789‑M1/149‑21 (MQ=255)                     cAGGTGCGCTGGAGTAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGGCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAAattccatcaggtaaagcttcccagc                                                                                                                            <  2:268921‑M1/149‑26 (MQ=255)                             cTGGAGAAGGCCAGAACCAGACAGAGCGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCACAAAAACCCTCCCCGTTa                                                                                                                    <  1:37732/149‑1 (MQ=255)                                      gCCAGAACCAGAGAGAGCGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAAattccatcaggtaaagcttcccagcgacgtatcagtcaccgt                                                                                                           <  1:282788‑M1/149‑43 (MQ=255)                                                    gagCGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAAattccatcaggtaaagcttcccagcgacgtatcagtcaccgtagacattaaagtcg                                                                                             <  2:246664‑M1/149‑57 (MQ=255)                                                             cACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACATGTTTGTTACACTTATCGTGCCATAAAAattccatcaggtaaagcttcccagcgacgtatcagtcaccgtagacattaaagtcgaaatacttt                                                                                    >  2:175762‑M1/1‑84 (MQ=255)                                                                cggttTTTTCATAATGTGCTGAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAAattccatcaggtaaagcttcccagcgacgtatcagtcaccgtagacattaaagtcgaaatactttttc                                                                                 <  1:165288‑M1/147‑69 (MQ=255)                                                                         cATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAAattccatcaggtaaagcttcccagcgacgtatcagtcaccgtagacattaaagtcgaaatactttttcgccatcttg                                                                        >  2:184664‑M1/1‑72 (MQ=255)                                                                                               cTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAACattccatcaggtaaagcttcccagcgacgtatcagtcaccgtagacattaaagtcgaaatactttttcgccatcttgtcgtaggtgccgtcctgacgca                                                  <  1:267245‑M1/149‑100 (MQ=255)                                                                                                                ttACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAAATATCCCGACCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTAAATGCACCACGATAGTGCAACAAACCTGGCATGCACAatgat                                 >  2:21151/1‑149 (MQ=255)                                                                                                                   cAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCATgatgat                              >  2:213914/1‑149 (MQ=255)                                                                                                                     tgtTTGTTACACTTATCGTGCCATAAAAattccatcaggtaaagcttcccagcgacgtatcagtcaccgtagacattaaagtcgaaatactttttcgccatcttgtcgtaggtgccgtcctgacgcagctcgccaagcgccttattgaa                            <  1:175762‑M1/148‑122 (MQ=255)                                                                                                                                 ttATCGTGCCATAAAAattccatcaggtaaagcttcccagcgacgtatcagtcaccgtagacattaaagtcgaaatactttttcgccatcttgtcgtaggtgccgtcctgacgcagctcgccaagcgccttattgaaggcagccgtccg                >  2:124171‑M1/1‑16 (MQ=255)                                                                                                                                               aaTTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCATGATGATCATTCCATCAGGTACAGCTTCCCAGCGa  >  2:55984/1‑149 (MQ=255)                                                                                                                                                |                                                                                                                                                    CGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGCGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCATGATGATCATTCCATCAGGTACAGCTTCCCAGCGA  >  CP009273/2420193‑2420483

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.