| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | CP009273 | 337,558 | C→G | Y241* (TAC→TAG) | yahJ → | putative metallo‑dependent hydrolase domain deaminase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 337,558 | 0 | C | G | 98.3% | 180.0 / NA | 58 | Y241* (TAC→TAG) | yahJ | putative metallo‑dependent hydrolase domain deaminase |
| Reads supporting (aligned to +/- strand): ref base C (0/1); new base G (34/23); total (34/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.14e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AAAAATCTGCAAAATTTGCAGGCGGTGCTGGCGCGACGTCAGGCGGGCTTTGAGTGTGAAATCGTCGCCTTCCCGCAGCACGGTTTGCTGCTGTCGAAATCTGAACCTTTAATGCGTGAAGCGATGCAGGCGGGGGCGCATTACGTCGGCGGCCTGGACCCGACCAGTGTTGATGGCGCGATGGAAAAATCCCTCGACACCATGTTCCAGATTGCGCTGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCA > CP009273/337415‑337697 | aaaaaTCTGCAAAATTTGCAGGCGGTGCTGGCGCGACGTCAGGCGGGCTTTGAGTGTGAAATCGTCGCCTTCCCGCAGCACGGTTTGCTGCTGTCGAAATCTGAACCTTTAATGCGTGAAGCGATGCAGGCGGGGGCGCATTAGGTcgg > 1:918965/1‑149 (MQ=255) tCTGCAAAATTTGCAGGCGGTGCTGGCGCGACGTCAGGCGGGCTTTGAGTGTGAAATCGTCGCCTTCCCGCAGCACGGTTTGCTGCTGTCGAAATCTGAACCTTTAATGCGTGAAGCGATGCAGGCGGGGGCGCATTAGGTcgg < 2:553684/144‑1 (MQ=255) tGCAAAATTTGCAGGCGGTGCTGGCGCGACGTCAGGCGGGCTTTGAGTGTGAAATCGTCGCCTTCCCGCAGCACGGTTTGCTGCTGTCGAAATCTGAACCTTTAATGCGTGAAGCGATGCAGGCGGGGGCGCATTAGGTcggcg < 2:833980/144‑1 (MQ=255) gCAAAATTTGCAGGCGGTGCTGGCGCGACGTCAGGCGGGCTTTGAGTGTGAAATCGTCGCCTTCCCGCAGCACGGTTTGCTGCTGTCGAAATCTGAACCTTTAATGCGTGAAGCGATGCAGGCGGGGGCGCATTAGGTcggcgg > 2:947359/1‑144 (MQ=255) acttttGCAGGCAGTGCTGGCGCGACGTCAGGCGGGCTTTGAGTGTGAAATCGTCGCCTTCCCGCAGCACGGTTTGCTGCTGTCGAAATCTGAACCTTTAATGCGTGAAGCGATGCAGGCGGGGGCGCATTAGGTCGGCGGCCTGGAcc < 1:481467/146‑1 (MQ=255) tttGCAGGCGGTGCTGGCGCGACGTCAGGCGGGCTTTGAGTGTGAAATCGTCGCCTTCCCGCAGAAAGGTTTGCTGCTGTCGAGATCTGAACCTTTAATGCGTGAAGCGATGCAGGCGGGGGCGCATCAGGTCGGCGGCCTGGa > 2:59987/1‑144 (MQ=255) gCGGTGCTGGCGCGACGTCAGGCGGGCTTTGAGTGTGAAATCGTCGCCTTCCCGCAGCACGGTTTGCTGCTGTCGAAATCTGAACCTTTAATGCGTGAAGCGATGCAGGCGGGGGCGCATCAGGTCGGCGGCCTGGACCCGAcg > 2:58621/1‑143 (MQ=255) cGGTGCTGGCGCGACGTCAGGCGGGCTTTGAGTGTGAAATCGTCGCCTTCCCGCAGCACGGTTTGCTGCTGTCGAAATCTGAACCTTTAATGCGTGAAGCGATGCGGGCGGGGGCGCATTAGGTCGGCGGCCTGGACCCGCCCa > 2:339585/1‑144 (MQ=255) tGCTGGCGCGACGTCAGGCGGGCTTTGAGTGTGAAATCATCGCCTTCCCGCAGCACGGTTTGCTGCTGTCGAAATCTGAACCTTTAATGCGTGAAGCGATGCAGGCGGGGGCGCATTAGGTCGGCGCCCTGGACCCGACCAggg > 2:707825/1‑142 (MQ=255) ggCGCGCCGTCAGGGGGGCTTGGAGTGTGAAATCGTCGCCTTCCCGCAGCACGGTTTGCTGCTGTCGAAATCTGAACCTTTAATGCGTGAAGCGATGCAGGCGGGGGCGCATTAGGTCGGCGGCCTGGACCCGACCAGTGTTGa < 2:629806/144‑1 (MQ=255) ggCGCGACGTCAGGCGGGCTTTGAGTGTGAAATCGTCGCCTTCCCGCAGCACGGTTTGCTGCTGTCGAAATCTGAACCTTTAATGCGTGAAGCGATGCAGGCGGGGGCGCATTAGGTCGGCGGCCTGGACCCgcacaggggtga > 2:452477/1‑133 (MQ=255) ggCGCGACGTCAGGCGGGCTTTGAGTGTGAAATCGTCGCCTTCCCGCAGCACGGTTTGCTGCTGTCGAAATCTGAACCTTTAATGCGTGAAGCGATGCAGGCGGGGGCGCATTAGGTCGGCGGCCTGGACCCGACCAGTGTTGATGgcg < 1:622991/149‑1 (MQ=255) ggcgACGGCGGGCGGGCTTTGGGTGTGAAATCGTCGCCTTCCCGCAGCACGGTTTGCTGCTGTCGAAATCTGAACCTTTAATGCGTGAAGCGATGCAGGCGGGGGCGCATTAGGTCGGCGGCCTGGACCCGACCAGTGTTGATg < 2:241562/143‑1 (MQ=255) aGGCGGGCTTTGAGTGTGAAATCGTCGCCTTCCCGCAGCACGGTTTGCTGCTGTCGAAATCTGAACCTTTAATGCGTGAAGCGATGCAGGCGGGGGCGCATTAGGTCGGCGGCCTGGACCCGACCAGTGTTGATGGCGCGATGGaaaaa > 1:970397/1‑149 (MQ=255) gCGGGCTTTGAGTGTGAAATCGTCGCCTTCCCGCAGCACGGTTTGCTGCTGTCGAAATCTGAACCTTTAATGCGTGAAGCGATGCAGGCGGGGGCGCATTAGGTCGGCGGCCTGGGCCCGAGCAGTGTTGATGGCGGGATGGAAAAATc > 1:321653/1‑149 (MQ=255) gCGGGCTTTGAGTGTGAAATCGTCGCCTTCCCGCAGCACGGTTTGCTGCTGTCGAAATCTGAACCTTTAATGCGTGAAGCGATGCAGGCGGGGGCGCATTAGGTCGGCGGCCTGGACCCGACCAGTGTTGATGGCGCGATGGaa < 2:836663/144‑1 (MQ=255) ggCTTTGAGTGTGAAATCGTCGCCTTCCCGCAGCACGGTTTGCTGCTGTCGAAATCTGAACCTTTAATGCGTGAAGCGATGCAGGCGGGGGCGCATTAGGTCGGCGGCCTGGACCCGACCAGTGTTGATGGCGCGATGGAAAAATCCCt > 1:514447/1‑149 (MQ=255) ttGAGTGTGAAATCGTCGCCTTCCCGCAGCACGGTTTGCGGCTGTCGAAATCTGAACCTTTAATGCGTGAAGCGATGCAGGCGGGGGCGCATTAGGTCGGCGGCCTGGACCCGACCAGTGTTGATGGCGCGATGGAAAAATCCCTCGac < 1:165796/149‑1 (MQ=255) aGTGTGAAATCGTCGCCTTCCCGCAGCACGGTTTGCTGCTGTCGAAATCTGAACCTTTAAGGCGTGAAGCGATGCAGGCGGGGGCGCATTAGGTCGGCGGCCTGGACCCGACCAGGGTTGATGGCGCGATGGAAAAATCCCTCg > 2:906114/1‑144 (MQ=255) tgtgAAATCGTCGCCTTCCCGCAGCACGGTTTGCTGCTGTCGAAATCTGAACCTTTAATGCGTGAAGCGATGCAGGCGGGGGCGCATTAGGTCGGCGGCCTGGACCCGACCAGTGTTGATGGCGCGATGGAAAAATCCCTCGACCCCAt > 1:696750/1‑149 (MQ=255) gAAATCGTCGCCTTCCCGCAGCACGGTTTGCTGCTGTCGAAATCTGAACCTTTAATGCGTGAAGCGATGCAGGCGGGGGCGCATTAGGTCGGCGGCCTGGACCCGACCAGTGTTGATGGCGCGATGGAAAAATCCCTCGACACCATGtt > 1:462930/1‑149 (MQ=255) caTCGTCGCCTTCCCGCAGCAAGGGTTGCTGCTGTCCAACTCGCAACGTTTAATGCGTGAAGCGATGCAGGCGGGGCCGCATTAGGTCGGCGGCCCGGACCCGGCCCGTGTTGATGGCGCGATGGAAAAATCCCTCGACACCAt < 2:379448/143‑1 (MQ=255) tcgCCTTCCCGCAGCACGGTTTGCTGCTGTCGAAATCTGAACCTTTAATGCGTGAAGCGATGCAGGCGGGGGCGCATTAGGTCGGCGGCCTGGACCCGACCAGTGTTGATGGCGCGATGGAAAAATCCCTCGACACCATGTTCCAGAtt > 1:394633/1‑149 (MQ=255) gCCTTCCCGCAGCACGGTTTGCTGCTGTCGAAATCTGAACCTTTAATGCGTGACGCGCTGCAGGCGGGGGCGCACTAGGTCGGCGAGCTGGACCCGACCAGTGGTGAAGGCGCGATGGAGAGATGCACCGACACCAGGTTCAAg > 2:303186/1‑144 (MQ=255) cTTCCCGCAGCACGGTTTGCTGCTGTCGAAATCTGAACCTTTAATGCGTGAAGCGATGCAGGCGGGGGCGCATTAGGTCGGCGGCCTGGACCCGACCAGTGTTGATGGCGCGATGGAAAAATCCCTCGACACCATGTTCCAGAt > 2:420702/1‑144 (MQ=255) gcagcaCGGTTTGCTGCTGTCGAAATCTGAACCTTTAATGCGTGAAGCGATGCAGGCGGGGGCGCATTAGGTCGGCGGCCTGGACCCGACCAGTGTTGATGGCGCGATGGAAAAATCCCTCGACACCATGTTCCAGATTGCGCTGGACt > 1:452605/1‑149 (MQ=255) gcagcaCGGTTTGCTGCTGTCGAAATCTGAACCTTGAATGCGTGAAGCGATGCAGGCGGGGGCGCATTAGGTCGGCGGCCTGGACCCGGCCAGTGTTGATGGCGCGAAGGAAAAATCCCTCGACACCATGTTCCAGATTGCGCt < 2:840639/144‑1 (MQ=255) gcaCGGGTTGCTGCTGTCGAAATCTGAACCTTTAATGCGTGTCGCGATGCAGGCGGGGGCGCATTAGGTCGGCGGCCTGGACCCGCCCAGTGTTGATGGCGCGATGGAAAAATCCCTCGACACCATGTTCCAGATTGCGCTGGa < 2:353612/144‑1 (MQ=255) caCGGTTTGCTGCTGTCGAATTCTGAACCGTTAATGCGTGAAGCGATGCAGGCGGGGGCGCATTAGGTCGGCGGCCTGGACCCGACCAGTGTTGATGGCGCGATGGAAAAATCCCTCGACACCATGTTCCAGCTTGCGCTGGAc > 2:490713/1‑144 (MQ=255) caCGGTTTGCTACTGACGAAATCTGAACCTTTAGTGCGTGAAGCGGTGCGGGCGGGGGCGCATTAGGTCGGCGGCCTGGACCCGACCAGGGTTGATGGCGCGATGGAAAAATCCCTCGACACCATGTTACAGATTGCGCTGGAc > 2:520460/1‑144 (MQ=255) gTTTGCTGCTGTCGAAATCTGAACCTTTAATGCGTGAAGCGATGCAGGCGGGGGCGCATTAGGTCGGCGGCCTGGACCCGACCAGTGTTGATGGCGCGATGGAAAAATCCCTCGACACCATGTTCCAGATTGCGCTGGACTACGACaaa < 1:380517/149‑1 (MQ=255) tgctgcTGTCGAAATCTGAACCTTTGATGCCGGAAGCGATGCCGGCGGGGGCGCAGTAGGTCGGCGGCCTGGACCCGACCAGTGTTGATGGCGCGATGGAAAAATCCCTCGACACCATGTTCCAGATTGCGCTGGACTACGACAAAGGc < 1:1025683/149‑1 (MQ=255) gTCGAAATCCGAACCTTTAATGCGTGAAGCGATGCAGGCGGGGGCGCATTAGGTCGGCGGCCTGGACCCGACCAGTGTTGATGGCGCGATGGAAAAATCCCTCGACACCATGTTCCAGATTGCGCTGGACTACGACAAAGGCGTCGata < 1:283318/149‑1 (MQ=255) gAAATCTGAACCTTTAATGCGTGAAGCGATGCAGGCGGGGGCGCATTAGGTCGGCGGCCTGGACCCGACCAGTGTTGATGGCGCGGTGGAAAAATCCCTCGACACCATGTTCCAGATTGCGCTGGACTACGACAAAGGCGTCGa > 2:339213/1‑144 (MQ=255) gAAATCTGAACCTTTAATGCGTGAAGCGATGCAGGCGGGGGCGCATTAGGTCGGCGGCCTGGACCCGACCAGTGTTGATGGCGCGATGGAAAAATCCCTCGACACCATGTTCCAGATTGCGCTGGACTACGACAAAGGCGTCGATATTc > 1:848296/1‑149 (MQ=255) aaaTCTGAACGTATAATGCGTGAAGCGATGCAGGCGGGGGCGCATTAGGTCGGCGGCCTGGACCCGACCAGTGTTGATGGCGCGATGGAAAAATCCCTCGACACCATGTTCCAGATTGCAGTGGACTACGACAAAGGCGGCGat > 2:890729/1‑144 (MQ=255) aaaTCTGAACCTTTAATGCGTGAAGCGATGCAGGCGGGGGCGCATTAGGTCGGCGGCCTGGACCCGACCAGTGTTGATGGCGCGATGGAAAAATCCCTCGACAACCTGTTCCAGAGTGCGCTGGACTACGACAAAGGCGTCGATCTTCa > 1:865332/1‑149 (MQ=255) aaTCTGAACCTTTAATGCGTGAAGCGATGCAGGCGGGGGCGCATTAGGTCGGCGGCCTGGACCCGACCAGTGTTGATGGCGCGATGGAAAAATCCCTCGACACCATGTTCCAGATTGCGCTGGACTACGACAAAGGCGTCGata < 2:193258/144‑1 (MQ=255) tCTGAGCCTTTGATGCGTGAAGCGATGCAGGCGGGGGCGCAGTAGGTCGGCGGCCTGGACCCGACCAGTGATAACGGCGCGCTCGATAAACCCCTCGTCACCATGTTCCAGATTGCGCTGGACTACGACAAAGGCGTCGATAtt < 2:558398/144‑1 (MQ=255) tCTGACCCTTTAATGCGCGAAGCGATGCAGGCGGGGGCGCATTAGGTCGGCGGCCTGGACCCGACCAGTCTTGATGCCGCCACGGCACAATCCCTCGACACCATGTTCCAGATTGCGCTGGACTACGACAAAGGCGTCGATAtt < 2:558294/144‑1 (MQ=255) tCTGAACCTTTAATGCGTGAAGCGATGCAGGCGGGGGCGCATTAGGTCGGCGGCCTGGACCCGACCAGTGTTGATGGCGCGATGGAAAAATCCCTCGACACCATGTTCCAGATTGCGGTGGACTCCGCCAAAAGCGTCGATATGCACCt > 1:1049542/1‑149 (MQ=255) gAACCTTTAATGCGTGAAGCGATGCAGGCGGGGGCGCATTAGGTCGGCGGCCTGGACCCGGCCAGTGTTGATGGCGCGATGGAAAAATCCCTCGACACCATGTTACAGATTGCCCTGGACTGCGACAAAGGCGTCGATATTCACCttcc > 1:789068/1‑146 (MQ=255) gAACCTTTAATGCGTGAAGCGATGCAGGAGGGTACGCATTAGGTCGGCGGCCTGGACCCGACCAGTGTTGATGGCGCGATGGAAAAATCCCTCGACACCATGTTCCAGATTGCGCTGGACTACGACAAAGGCGTCGATATTCAc > 2:725709/1‑144 (MQ=255) aaCCTTTAATGCGTGAAGCGATGCAGGCGGGGGCGCATTAGGTCGGCGGCCTGGACCCGACCAGTGTTGATGGCGCGATGGAAAAATCCCTCGACACCATGTTCCAGATTGCGCTGGACTACGACAAAGGCGTCGATATTCACCTGCAc > 1:1066559/1‑149 (MQ=255) tttAATGCGTGAAGCGATGCAGGCGGGGGCGCATTAGGTCGGCGGCCTGGACCCGACCAGTGTTGATGGCGCGATGGAAAAATCCCTCGACACCATGTTCCAGATTGCGCTGGACTACGACAAAGGCGTCGATATTCACCTGCACGaaa > 1:938163/1‑149 (MQ=255) tttAATGCGGGAAGCGATGCAGGCGGGGGCGCATTAGGTCGGCGGCCTGGACCCGACCAGTGTTGATGGCGCGATGGAAAAATCCCTCGACACCATGTTCCAGATTGCGCTGGACTACGACAAAGGCGTCGATATTCACCTGCACGaaa < 1:1031926/149‑1 (MQ=255) ttAACGCGTGAAGCGATGCAGGCGGGGGCGCATTAGGTCGGCGGCCTGGACCCGACCAGTGTTGATGGCGCGATGGAAAAATCCCTCGACACCATGTTCCAGATTGCGCTGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAAc < 1:445749/149‑1 (MQ=255) aTGCGTGAAGCGATGCAGGCGGGGGCGCATTAGGTCGGCGGCCTGGACCCGACCAGTGTTGATGGCGCGATGGAAAAATCCCTCGACACCATGTTCCAGATTGCGCTGGACTACGACAAAGGCGTCGATATTCACCTGCACGaa > 2:788020/1‑144 (MQ=255) tGCGTGAAGCGATGCAGGCGGGGGCGCATTAGGTCGGCGGCCTGGACCCGACCAGTGTTGATGGCGCGATGGAAAAATCCCTCGACACCATGTTCCAGATTGCGCTGGACTACGACAAAGGCGTCGATATTCACCTGCACGaaa < 2:406781/144‑1 (MQ=255) gCGTGAAGCGATGCAGGCGGGCGCGCATTAGGTCGGCGGCCTGGACCCGACCAGTGTTGATGGCGCGATGGAAAAATCCCTCGACACCATGTTCCAGATTGCGCTGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTc < 1:66886/149‑1 (MQ=255) gAAGCGATGCAGGCGGGGGCGCATTAGGTCGGCGGCCTGGACCCGACCAGTGTTGATGGCGCGATGGAAAAATCCCTCGACACCATGTTCCAGATTGCGCTGGACTACGACAAAGGCGTCGGTATTCACCTGCACGAAACCACTCCGGc > 1:1070083/1‑149 (MQ=255) aGGCGGGGGCGCATTAGGTCGGCGGCCTGGACCCGACCAGTGTTGATGGCGCGATGGAAAAATCCCTCGACACCATGTTCCAGATTGCGCTGGACTACGACAAAGGCGTCGATATTCACCTGCACGAACCCACTCCGGCAGGCGTGGCa > 1:979885/1‑149 (MQ=255) aGGCGGGGGCGCATTAGGTCGGCGGCCTGGACCCGACCAGTGTTGATGGCGCGATGGAAAAATCCCTCGACACCATGTTCCAGATTGCGCTGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCa > 1:928127/1‑149 (MQ=255) aGGCGGGGGCGCATTAGGTCGGCGGCCTGGACCCGACCAGTGTTGATGGCGCGATGGAAAAATCCCTCGACACCATGTTCCAGATTGCGCTGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCa > 1:58526/1‑149 (MQ=255) ggCGCATTAGGTCGGCGGCCTGGACCCGACCAGTGTTGATGGCGCGATGGAAAAATCCCTCGACACCATGTTCCAGATTGCGCTGGACTACGACAAAGGCGTCGATATTCACCTGCCCGAAACCACTCCGGCAGGCGTGGCAGc > 2:1022178/1‑144 (MQ=255) ggCGCATTAGGTCGGCGGCCTGGACCCGACCAGTGTTGATGGCGCGATGGAAAAATCCCTCGACACCATGTTCCAGATTGCGCTGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCa < 1:6337/149‑1 (MQ=255) ggCGCATCAGGTCGGCGGCCTGGACCCGACCAGTGTTGATGGCGCGATGGAAAAATCCCTCGACACCATGTTCCAGATTGCGCTGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCa < 1:65389/149‑1 (MQ=255) gcATTACGTCGGCGCCCTTGACCCGACCATTGTTGATGGCGCGATGGAAAAATCCCTCCACACCATGTTCCAGATTGCGCTGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCAt < 2:689779/144‑1 (MQ=255) | AAAAATCTGCAAAATTTGCAGGCGGTGCTGGCGCGACGTCAGGCGGGCTTTGAGTGTGAAATCGTCGCCTTCCCGCAGCACGGTTTGCTGCTGTCGAAATCTGAACCTTTAATGCGTGAAGCGATGCAGGCGGGGGCGCATTACGTCGGCGGCCTGGACCCGACCAGTGTTGATGGCGCGATGGAAAAATCCCTCGACACCATGTTCCAGATTGCGCTGGACTACGACAAAGGCGTCGATATTCACCTGCACGAAACCACTCCGGCAGGCGTGGCAGCCATCA > CP009273/337415‑337697 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |