Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009273 3,029,079 C→T A188T (GCT→ACT)  prfB ← peptide chain release factor RF‑2

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,029,0790CT95.8% 126.7 / NA 48A188T (GCT→ACT) prfBpeptide chain release factor RF‑2
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (17/29);  minor base G (2/0);  total (19/29)
Fisher's exact test for biased strand distribution p-value = 1.52e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGGTTGATTTCGATATCAATATCATCATCAACTTCCGGATAAACAAACGCGGAGCTGAACGACGTGTGGCGACGACCGCCGGAGTCAAACGGGCTTTTACGCACCAGGCGGTGAACGCCGGTTTCTGTACGCAGCCAGCCGTAAGCGTAATCGCCGGAGATTTTGATCGTCACGGATTTAATACCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGTTTTGAAACCACGCGATTCTGCCCAGCGCAGATACATACGCTCAAGCATGCTCGCCCAGTCCTGTGCTT  >  CP009273/3028934‑3029222
                                                                                                                                                 |                                                                                                                                               
gggTTGATTTCGATATCAATATCATCATCAACTTCCGGATAAACAAACGCGGAGCTGAACGACGTGTGGCGACGACCGCCGGAGTCAAACGGGCTTTTACGCACCAGGCGGTGAACGCCGGTTTCTGTACGCAGCCAGCCGTAAGTGTa                                                                                                                                              >  1:85150/1‑149 (MQ=255)
gggTTGATTTCGATATCAATATCATCATCAACTTCCGGATAAACAAACGCGGAGCTGAACGACGTGTGGCGACGACCGCCGGAGTCAAACGGGCTTTTACGCACCAGGCGGTGAACGCCGGTTTCTATACGCAGCCCGCCGTAAGTGTa                                                                                                                                              >  1:84968/1‑149 (MQ=255)
  gTTGATTTCGATATCAATATCATCATCAACTGCCGGATAAACAAACGCGGAGCTGAACGACGTGTGGCGACGACCGCCGGAGCCAAACGGGCTTTTACGCACCAGCCGGTGAACGCCGGTTTCGTTACGCTGACAGCCCTAAGGGTact                                                                                                                                            >  2:642423/1‑147 (MQ=255)
       tttCGATATCAATATCATCATCAACTTCCGGATAAACAAACGCGGAGCTGAACGACGTGTGGCGACGACCGCCGGAGTCAAACGGGCTTTTACGCACCAGGCGGTGAACGCCGGTTTCTGTACGCAGCCAGCCGTAAGTGTAATCGCCg                                                                                                                                       <  1:267395/149‑1 (MQ=255)
       tttCGATATCAATATCATCATCAACTTCCGGATAAACAAACGCGGAGCTGAACGACGTGTGGCGACGACCGCCGGAGTCAAACGGGCTTTTACGCACCAGGCGGTGAACGCCGGTTTCTGTACGCAGCCAGCCGTAAGGGTAATCGCCg                                                                                                                                       >  1:304867/1‑149 (MQ=255)
               tcaatatcaTCATCAACTTCCGGATAAACAAACGCGGAGCTGAACGACGTGTGGCGACGACCGCCGGAGTCAAACGGGCTTTTACGCACCAGGCGGTGAACGCCGGTTTCTGTACGCAGCCAGCCGTAAGTGTAATCCCCGGCGAtttt                                                                                                                               >  1:686556/1‑149 (MQ=255)
                caatatcaTCATCAACTTCCGGATAAACAAACGCGGAGCTGAACGACGTGTGACGACGACCGCCGGGGTCAAACGGGCTTTTACGCACCAGCCGGGGAACGCCGGTTGCTGTACGCAGCCGGCCGTAAGTGTAATCTCCGgagagtgtg                                                                                                                              >  1:317564/1‑144 (MQ=255)
                  atatcaTCATCAACTTCCGGATAAACAACCGCGGAGCTGAACGACGTGGGGCGACGACCGCCGGAGTCAAACGGGCTTTTACGCACCAGGCGGTGAACGCCGGTTTCTGTACGCAGCCAGCCGTAAGTGTAATCGCCGGAGATTTTGAt                                                                                                                            <  1:11103/149‑1 (MQ=255)
                          atccACTTCCGGATAAACAAACGGGGAGCTGAACGACGTGTGGCGACGACCGCCGGAGTCAAACGGGCTTTTACGCACCAGGCGGTGAACGCCGGTTTCTGTACGCGGCCAGCCGTAAGTGTAATCGCCGGAGATTTTGATCGTCACgg                                                                                                                    <  2:273794/149‑1 (MQ=255)
                                ttCCGGATAAACAAACGCGGAGCTGAACGACGTGTGGCGACGACCGCCGGAGTCAAACGGGCTTTTACGCACCAGGCGGTGAACGCCGGTTTCTGTACGCAGCCAGCCGTAAGTGTAATCGCCGGAGCTTTTGATCGTCACGGCTTTaa                                                                                                              >  1:273650/1‑149 (MQ=255)
                                ttCCGGATAAACAAACGCGGAGCTGAACGACGTGTGGCGACGACCGCCGGAGTCAAACGGGCTTTTACGCACCAGGCGGTGAACGCCGGTTTCTGTACGCAGCCAGCCGTAAGTGTAATCGCCGGAGATTTTGATCGTCACGGATTTaa                                                                                                              >  1:273739/1‑149 (MQ=255)
                                     gATAAACAAACGCGGAGCTGAACGACGTGTGGTGACGACCGCCGGAGTCAAACGGGCTTTTACGCACCAGGCGGTGAACGCCGGTTTCTGTACGCAGCCAGCCGTAAGTGTAATCGCCGGAGATTTTGATCGTCACGGATTTAATAccc                                                                                                         <  1:501359/149‑1 (MQ=255)
                                             agcgcgGAGCGGAACGACGTGTGGCGACGACCGCCGGAGTCAAACGGGCTTTTACGCACCAGGCGGTGAACGCCGGTTTCTGTACGCAGCCAGCTGGACGTGTAATCGCCGGAGATTTTGATCGTCACGGATTTAATACCCGCCACTTc                                                                                                 <  1:506958/147‑1 (MQ=255)
                                             aacGCGGAGCTGAACGACGTGTGGCGACGACCGCCGGAGTCAAACGGGCTTTTACGCACCAGGCGGTGAACGCCGGTTTCTGTACGCAGCCAGCCGTAAGTGTAATCGCCGGAGATTTTGATCGTCACGGATTTAATACCCGCCACTTc                                                                                                 <  1:608084/149‑1 (MQ=255)
                                                    cgCTTAACGACGTGTGGCGCCGACCGCCGGAGTCAAACGGGCTTTTACGCACCAGGCGCTGAACGCCAGTTTCTGTCCGCAGCCAGCCGTAAGTGTAATCGCCGGAGATTTTGATCGTCACGGATTTAATACCCGCCACTTCACCTTcc                                                                                          <  2:302416/148‑1 (MQ=255)
                                                         aaCGACGTGTGGCGACGACCGCCGGAGTCAAACGGGCTTTTACGCACCAGGCGGTGAACGCCGGTTTCTGTACGCAGCCAGCCGTAAGTGTAATCGCCGGAGATTTTGATCGTCACGGATTTAATACCCGCCACTTCACCTTCCGActc                                                                                     >  1:125016/1‑149 (MQ=255)
                                                                 gtgGCGACGACCGCCGGAGTCAAACGGGCTTTTCTGCACCAGGCGGTGAACGCCGGTTTCTGTACGCAGCCAGCCGTAAGTGTAATCGCCGGAGATTTTGATCGTCACGGATTTAATACCCGCCACTTCACCTTCCGACTCTTCgatga                                                                             <  2:249477/149‑1 (MQ=255)
                                                                 gcggCGACGACCGCCGGCGTCAAACGGGCTTTTACTCCCCCGGCGGTGAACGCCGGTTTCTGTACGCAGCCAGCCGTAAGTGTAATCGCCGGAGATTTTGATCGTCACGGATTTAATACCCGCCACTTCACCTTCCGACTCTTCgatga                                                                             <  2:588157/147‑1 (MQ=255)
                                                                     cgacgaCCGCCGGAGTCAAACGGGCTTTTACGCACCAGGCGGTGAACGCCGGTTTCTGTACGCAGCCAGCCGTAAGTGTAATCGCCGAAGATTTTGATCGTCACGGATTTAATACCCGCCACTTCCCCTTCCGACTCTGCGATGATTTc                                                                         >  1:105872/1‑149 (MQ=255)
                                                                      ggcgcgggccgcggTCAAACGGGGTGTTACGCACCAGGCGGTGAACGCCGGTTGCGGGCCGCAGCCAGCCGTAAGTGTAATCGCCGGAGATTTTTATCGTCACGGATTTAATCCCCGCCACTTCACCTTCCGACTCTTCGATGATTTCa                                                                        <  2:135976/136‑1 (MQ=255)
                                                                         tacCGCCGGAGTCAACCGGGATTTTACGCACCAGGCGGTGAACGCCGGTTTCTGTACGAAGCCAGCCGTAAGTGTAATCGCCGGAGATTTTGATCGTCACGGATTTAATGCCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGtt                                                                     <  1:465757/148‑1 (MQ=255)
                                                                          acCGCCGGAGTCAAACGGGCTTTTACGCACCAGGCGGTGAACGCCGGTTTCTGTACGCGGCCAGCCGTAAGTGTAATCGCCGGAGATTTTGATCGTCACGGATTGCATACCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGttt                                                                    <  2:567728/149‑1 (MQ=255)
                                                                           ccgccgGGGTCAAACGGGCTTTTACGCACCAGGCGGTGAACGCGGGTTTCTGTACGCAGCCAGCCGTAAGTGTAAGCGCCGGAGATTTTGATCGTCACGGATTTAATACCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGtttt                                                                   <  2:23959/149‑1 (MQ=255)
                                                                           ccgccgGAGTCAAACGGGCTTTTACGCACCAGGCGGGGAACGCTGGTTTCTGTACGCAGCCAGCCGTAAGTGTAATCGCCGGAGATTTTGATCGTCACGGATTTAATACCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGtttt                                                                   <  2:723657/149‑1 (MQ=255)
                                                                           ccgccgGAGTCAAACCGGCTTTTACGCACCAGGCGGTGAACGCCGGTTTCTGTACGCAGCCAGCCGTAAGTGTAATCGCCGGAGATTTTGATCGTCACGGATTTAATACCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGtttt                                                                   <  1:56804/149‑1 (MQ=255)
                                                                                ggAGTCAAACGGGCTTTTACGCACCAGGCGGTGAACGCCGGTTTCTGTACGCAGCCAGACGTAAGTGGAATCGCCGGAGATTTTGATAGTCCCGGATTTCGAAACCGCCACTGCACCTTCCGACTCTTCGGTGATTTCAGTTTTGAAAc                                                              >  2:166951/1‑149 (MQ=255)
                                                                                   gTCAAACGGGCTTTTACGCACCAGGCGGTGAACGCCGGTTTCTGTACGCAGCCAGCCGTAAGTGTAATCGCCGGAGATTTTGATCGTCACGGATTTAATACCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGTTTTGAAACCAc                                                           <  1:486877/149‑1 (MQ=255)
                                                                                    ccAAACGGGCTTTTACGCACCAGGCGGTGACCGCCGGTTTCTGTACGCAGCCAGCCGTAAGTGTAATCGCCGGAGATTTTGACCGTCACGGATTTAATACCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGTTTTGAAACCAcg                                                          <  2:108518/148‑1 (MQ=255)
                                                                                      aaaCGGGCTTTTACGCACCAGGCGGTGAACGCCGGTTTCTGTACGCAGCCAGCCGTAAGTGTAATCGCCGGAGATTTTGATCGTCACGGATTTAATACCCGCCACTTCACCTTCCGACTCTTCGATGATTTCCGTTTTGGAACCAcgcg                                                        >  2:444217/1‑149 (MQ=255)
                                                                                                  aCGCACCAGGCGGTGAACGCCGGTTTCTGTACGCAGCCAGCCGTAAGTGTAATCGCCGGAGATTTTGATCGTCACGGATTTAATACCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGTTTTGAAACCACGCGATTCTGCCCAgc                                            <  1:138505/149‑1 (MQ=255)
                                                                                                   cgtaCCGGGCGGTGAACGCCGGTTCCTGTACTCTGCAAGCCGTAAGTGGAATCGCCGGAGATTTTGATCTTCATGAATTCAATACCCGCCACTTCACATTTCGACTCTTCGATGATTTCGGTTTTGAAACCACGCGATTCTGCCCAgcg                                           <  2:85150/146‑1 (MQ=255)
                                                                                                   cGCACCAGGCGTGGAATGCCGGTTTCTGTACGCAGCCAGCCTGAACTGTAATCGCGGGAGATTTTGTTCGTCCCTGTTTTAATACCCTCCACTTCGCCGTCCGACTCTTCGATGATTTCAGTTTTGAAACCACGCGATTCTGCCCAgcg                                           <  2:84968/149‑1 (MQ=255)
                                                                                                   cGCACCACGCGGTGACAGCCGGCTTCTGTACGCCACCAGCCGTAAGTGTAATATCCGGAGATTTTAATCGTCACGGATTTAGTAGCCGCCACTTCCCCTCCCGAGTCATCGCTGATTTCAGTTTTGAAACCACCCGATTCTGCCCAgcg                                           >  2:283252/1‑149 (MQ=255)
                                                                                                        cAGGCGTCGAACGCCGGTTTCTGTACGCAGCCAGCCGTAAGTGTAATCGCCGGAGATTTTGATCGTCACGGATTTAATACCCGCCACTTCCCCTTCCGACTCTTCGATGATTTCAGTTTTGAAACCACGCGATTCTGCTCAGCGCAGat                                      <  2:637659/149‑1 (MQ=255)
                                                                                                          ggCGGTGAACGCCGGTTCCTGTACGCAGCCAGCCGTAAGTGTAATCGCCGGAGATTTTGATCGTCACGGATTTAATACCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGTTTTGAAACCACGCGATTCTGCCCAGCGCAGatac                                    <  1:459715/149‑1 (MQ=255)
                                                                                                          ggCGGGGAACGCCCGTTTCTGTACGAAGCCAGCCGTACGTGTAATCGCCGGAGATTTTGATCGTCACGGATTTAATACCCGCCACTTCACCTTCCGTCTCTTCGATGATTTCAGTTTTGAAACCACGCGATTCTGCCCAGCGCAGatac                                    <  1:405782/149‑1 (MQ=255)
                                                                                                             ggTGAACGCCGGTTTCTGTACGCAGCCAGCCGTAAGTGTAATCGCCGGAGATTTTGATCGTCACGGATTTAATACCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGTTTTGAAACCACGCGATTCTGCCCAGCGCAGatacata                                 <  1:667919/149‑1 (MQ=255)
                                                                                                                    gCCGGTTTCTGTACGCAGCCAGCCGTAAGTGTAATCGCCGGAGATTTTGATCGTCACGGATTTAATACCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGTTTTGAAACCACGCGATTCTGCCCAGCGCAGATACATACGCTCaa                          >  1:72398/1‑149 (MQ=255)
                                                                                                                    gCCGGTTTCTGTACGCAGCCAGCCGTAAGTGTAATCGCCGGAGATTTTGATCGTCACGGATTTAATACCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGTTTTGAAACCACGCGATTCTGCCCAGCGCAGATAAATACGCTCaa                          >  2:400538/1‑149 (MQ=255)
                                                                                                                    gCCGGTTTCGGTACGCTCCCAGCCGGAAGTGTAATCGCCGGTGATTTTGATCGTCACGGATGTAATACCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGTTTTGAAACCACGCGATTCTGCCCAGCGCAGATACATACGCTCaa                          <  1:4251/149‑1 (MQ=255)
                                                                                                                          ttCTGTACGCAGCCAGCCGTAAGTGTAATCGCCGGAGATTTTGATCGTCACGGATTTAATACCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGTTTTGAAACCACGCGATTCTGCCCAGCGCAGATACATACGCTCAAGCATGc                    <  1:289078/149‑1 (MQ=255)
                                                                                                                            cTGTCCGCACCCAGCCGTAAGTGCAATCGCCGGAGATTTTGACCGTCACGGATTTAATACCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGTTTTGAAACCACGCGATTCTGCCCAGCGCAGATACATACGCTCAAGCATGCTc                  <  2:731263/149‑1 (MQ=255)
                                                                                                                              gTACGCAGCCAGCCGTAAGTGTAATCGCCGGAGATTTTGATCGTCACGGATCTAATACCCGCCACTTCACCTTCCCACTCTTCGATGATTTCAGTTTTGATACCACGCGATTCTGCCCAGCGCATATACATAAGCTCAAACATGCTccc                >  2:286859/1‑147 (MQ=255)
                                                                                                                                 cGCAGCCAGCCGTAAGTGTAATCGCCGGAGATTTTGATCGTCACGGATTTAATACCCGCCACTTCACCTTCCGACTCTTCGATGATTTGAAAGCAGAAACCACGCGATTCTGCCCAGCGCAGATACATACGCTCAAGCATGCTCGCCCa             >  1:721414/1‑149 (MQ=255)
                                                                                                                                      ccagccGTAAGTGTAATCGCCGGAGATTTTGATCGTCACGGATTTAATACCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGTTTTGAAACCACGCGATTCTGCCCAGCGCAGATACATACGCTCAAGCATGCTCGCCCAGTCCt        >  1:159142/1‑149 (MQ=255)
                                                                                                                                        agccGTAAGTGTAATCGCCGGAGATTTTGATCGTCACGGATTTAATACCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGTTTTGAAACCACGCGATTCTGCCCAGCGCAGATACATACGCTCAAGCATGCTCGCCCAGTCCtgt      >  2:99621/1‑149 (MQ=255)
                                                                                                                                           cGTAAGTGTAATCGCCGGAGATTTTGATCGTCACGGATTTAATACCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGTTTTGAAACCACGCGATTCTGCCCAGCGCAGATACATACGCTCAAGCATGCTCGCCCAGTCCTGTGCt   <  2:149210/149‑1 (MQ=255)
                                                                                                                                            gTAAGTGTAATCGCCGGAGATTTTGATCGTCACGGATTTAATACCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGTTTTGAAACCACGCGATTCTGCCCAGCGCAGATACATACGCTCAAGCATGCTCGCCCAGTCCTGTGCtt  <  1:67277/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                               
GGGTTGATTTCGATATCAATATCATCATCAACTTCCGGATAAACAAACGCGGAGCTGAACGACGTGTGGCGACGACCGCCGGAGTCAAACGGGCTTTTACGCACCAGGCGGTGAACGCCGGTTTCTGTACGCAGCCAGCCGTAAGCGTAATCGCCGGAGATTTTGATCGTCACGGATTTAATACCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGTTTTGAAACCACGCGATTCTGCCCAGCGCAGATACATACGCTCAAGCATGCTCGCCCAGTCCTGTGCTT  >  CP009273/3028934‑3029222

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑