Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009273 753,013 G→T E551* (GAA→TAA)  sdhA → succinate dehydrogenase, flavoprotein subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273753,0130GT96.1% 148.5 / NA 51E551* (GAA→TAA) sdhAsuccinate dehydrogenase, flavoprotein subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base T (26/23);  minor base C (0/2);  total (26/25)
Fisher's exact test for biased strand distribution p-value = 2.35e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTTCCAGCGAGTTCAACACCCAGCGCGTTGAGTGCCTGGAACTGGATAACCTGATGGAAACGGCGTATGCAACGGCTGTTTCTGCCAACTTCCGTACCGAAAGCCGTGGCGCGCATAGCCGCTTCGACTTCCCGGATCGTGATGATGAAAACTGGCTGTGCCACTCCCTGTATCTGCCAGAGTCGGAATCCATGACGCGCCGAAGCGTCAACATGGAACCGAAACTGCGCCCGGCATTCCCGCCGAAGATTCGTACTTACTAATGCGGAGACAGGAAAATGAGACTCGAGT  >  CP009273/752867‑753157
                                                                                                                                                  |                                                                                                                                                
cgtccacggAGTGCACCCCCCAGCGCGCTGAGTGCCTGGAACTGGATAACCTGATGGAAACGGCGTATGCAACGGCTGTTTCTGCCAACTTCCGTACCGAAAGCCGTGGCGCGCATAGCCGCTTCGACTTCCCGGATCGTGATGATTaa                                                                                                                                                <  2:477048/141‑1 (MQ=255)
cTTCCAGCGAGTTCAACACCCAGCGCGTTGAGCGACTGAACCTCATTCGCTTGAATGAAACGGCGTATGCAACGGCTGTTTCTGCCAACTTCCGTACCGAAAGCCGTGGCGCGCATAGCCGCTTCGACTTCCCGGATCGTGATGATTaa                                                                                                                                                <  1:240584/149‑1 (MQ=255)
 ttCCAGCGAGTTCAACACCCAGCGCGTTGAGTGCCTGGAACTGGATAACCTGATGGAAACGGCGTATGCAACGGCTGTTTCTGCCAACTCCCGTACCGAAAGCCGTGGCGCGCATAGCCGCTTCGACTTCCCGGATCGTGATGATTaaa                                                                                                                                               >  2:317109/1‑149 (MQ=255)
  tCCAGCAAGTTCAACACCCAGGGCGTTGAGGGCATGGAACTGGATAACCTGATGGAAACGGCGTATGCAACGGCTGTTTCTGCCAACTTCCGTACCGAAAGCCGTGGCGCGCATAGCCGCTTCGACTTCCCGGATCGTGATGATTaaaa                                                                                                                                              <  2:372813/149‑1 (MQ=255)
     aGCGAGTTCAACACCCAGCGCGTTGAGTGCCTGGAACTGGATAACCTGATGGAAAGGGGGTGGGCAACGGCTGTTTCTGCCAACTTCCGTACCGAAAGCCGTGGCGCGCATAGCCGCTTCGACTTCCCGGATCGTGATGATTAAAACTg                                                                                                                                           >  1:390733/1‑149 (MQ=255)
        gAGTTCAACACCCAGCGCGTTGAGTGCCTGGAACTGGATAACCTGATGGAAACGGCGTATGCAACGGCTGTTTCTGCCAACTTCCGTACCGGAAGCCGTGGCGCGCATAGCCGCTTCGCCTTCCCGGATCGTGATGATTAAAACTGGCt                                                                                                                                        >  1:307853/1‑149 (MQ=255)
                   ccAGCGCGTCGGGTGCCGCGAACTGGCTACCCTGCTGGAGTCGGTGTATGCCACGGCTGTGTCTGCCAACTTCCGTACCGAAAGCCGGGGCGCGCAGGGCCGGTTCGACTTCCCGGAGCGTGATGATTAAACCTGGCTGTGCCACTccc                                                                                                                             <  2:577061/149‑1 (MQ=255)
                    cAGCGCGTTGAGTGCCTGGAACTGGATAACCTGATGGAAACGGCGTATGCAACGGCTGTTTCTGCCAGCTTCCGTACCGAAAGCCGTGGCGCGCATAGCCGCTTCGACTTCCCGGATCGTGATGATTAAAACTGGCtggccccccccct                                                                                                                            >  2:41260/1‑138 (MQ=255)
                         cgTTGAGTGCCTGGAACTGGATAGCCTGATGGAAACGGCGTATGCAACGGCTGTTTCTGCCAACTTCCGTACCGAAGGCCGTGGCGCGCATAGCCGCTTCGACTTCCCGGATCGTGATGATTAAAACTGGCTGTGCCACTCCCTGTATc                                                                                                                       <  2:529858/149‑1 (MQ=255)
                          gTTGAGGGCCTGGAACTGGATAACCTGATGGAAACGGCGTATGCAACGGCTGTTTCTGCCAACTTCCGTACCGAAAGCCGTGGCGCGCACAGCCGCTTCGACTTCCCGGATCGTGATGATTAAAACTGGCTGTGCCACTCCCTGTATCt                                                                                                                      <  1:489749/149‑1 (MQ=255)
                                   cTGGAACTGGATAACCTGATGGAAACGGCGTATGCAACGGCTGTTTCTGCCAACTTCCGTACCGAAAGCCGTGGCGCGCATAGCCGCTTCGACTTCCCGGATCGTGATGGTTAAAACTGGCTGTGCCACCCCCTGTTTCTGCCagagac                                                                                                             >  2:311491/1‑147 (MQ=255)
                                    tGGAACTGGATAACCTGATGGAAACGGCGTATGCAACGGCTGTTTCTGCCAACTTCCGTACCGAAAGCCGTGGCGCGCATAGCCGCTTCGACTTCCCGGATCGTGATGGTTAAAACTGGCTGTGCCACTCCCTGTATCTGCCCGAGACg                                                                                                            >  2:636233/1‑149 (MQ=255)
                                    tGGAACTGGATAACCTGATGGAAACGGCGTATGCAACGGCTGTTTCTGCCAACTTCCGTACCGAAAGCCGTGGCGCGCATAGCCGCTTCGACTTCCCGGATCGTGATGATTAAAACTGGCTGTGCCACTCCCTGTATCTGCCAGAGTCg                                                                                                            >  2:729319/1‑149 (MQ=255)
                                    tGGAACTGGATAACCTGATGGAAACGGCGTATGCAACGGCTGTTTCTGCCAACTTCCGTACCGAAAGCCGTGGCGCGCATAGCCGCTTCGACTTCCCGGATCGTGAGGATTAAAACTGGCTGTGCCACTCCCTGTAGCTGCCAGAGtgg                                                                                                            >  2:636289/1‑147 (MQ=255)
                                            gATAACCTGATGGAAACGGCGTATGCAACGGCTGTTTCTGCCAACTTCCGTACCGAAAGCCGTGGCGCGCATAGCCGCTTCGACTTCCCGGATCGTGATGATTAAAACTGGCTGTGCCACTCCCTGTATCTGCCAGAGTCGGAATCCAt                                                                                                    <  1:293221/149‑1 (MQ=255)
                                                aCCTGATGGAAACGGCGTATGCAACGGCTGTTTCTGCCAACTTCCGTACCGAAAGCCGTGGCGCGCATAGCCGCTTCGACTTCCCGGATCGTGATGATTAAAACTGGCTGTGCCACTCCCTGTGTCTGCCAGAGTCGGAATCCATGAcg                                                                                                >  1:82633/1‑149 (MQ=255)
                                                            cGGCGTATGCAACGGCTGTTTCTGCCAACTTCCGTACGGAAAGCCGTGGCGCGCATAGCCGCTTCGACTTCCCGGATCGTGATGATTAAAACTGGCTGTGCCACTCCCTGTATCTGCCAGAGTCGGAATCCATGACGCGCCGAAGCGTc                                                                                    <  1:562278/149‑1 (MQ=255)
                                                            cGGCGTATGCAACGGCTGTTTCTGCCAACTTCCGTACCGAAAGCCGTGGCGCGCATAGCCGCTTCGACTTCCCGGATCGTGATGATTAAAACTGGCTGTGCCACTCCCTGTATCTGCCAGAGTCGGAATCCATGACGCGCCGAAGCGTc                                                                                    <  1:577123/149‑1 (MQ=255)
                                                             ggCGTATGCAACGGCTGTTTCTGCCAACTTCCGTACCGAAAGCCGTGGCGCGCATAGCCGCTTCGACTTCCCGGATCGTGATGATTAAAACTGGCTGTGCCACTCCCTGTATCTGCCAGAGTCGGAATCCATGACGCGCCGAAGCGTCa                                                                                   >  1:658363/1‑149 (MQ=255)
                                                                  aTGCAACGGCTGTGCCTGCCAACTTCCGTCCCGAAAGCCGTGGCGCGCATAGCCGCTTCGACTTCCCGGATCGTGATGATTAAAACTGGCTGTGCCACTCCCTGTATCTGCCAGAGTCGGAATCCATGACGCGCCGAAGCGTCAACATg                                                                              <  1:255596/149‑1 (MQ=255)
                                                                    gCAACGGCTGTTTCTGCCAACTTCCGTACCGAAAGCCGTGGCGCGCATAGCCGCTTCGACTTCCCGGATCGTGATGATTAAAACTGGCTGTGCCACTCCCTGTATCTGCCAGAGTCGGAATCCATGACGCGCCGAAGCGTCGACCTGGa                                                                            >  2:675844/1‑149 (MQ=255)
                                                                     cAACGGCTGTTTCTGCCAACTTCCCTACCGAAAGCCGTGGCGCGCATAGCCGCTTCGACTTCCCGGATCGTGATGATTAAAACTGGCTGTGCCACTCCCTGTATCTGCCAGAGTCGGAATCCATGACGCGCCGAAGCGTCAACATGGaa                                                                           <  1:94172/149‑1 (MQ=255)
                                                                      aaCGGCTGTTTCTGCCAACTTCCGTACCGAAAGCCGTGGCGCGCATAGCCGCTTCGACTTCCCGGATCGTGATGATTAAAACTGGCTGTGCCACTCCCTGTATCTGCCAGAGTCGGAATCCATGACGCGCCGAAGCGTCAAACTGGAAc                                                                          >  1:534022/1‑149 (MQ=255)
                                                                      aaCGGCTGTTTCGGCCAACTTCCGTACCGAAAGCCGTGGCGCGCATCGCCGCTTCGACTTCCCGGATCGTGATGATTAAAACTTGCTGTGCCACTCCCTGTATCTGCCAGAGTCGGAATCCATGACGCGCCGAAGCGTCAACATGGAAc                                                                          <  1:311491/149‑1 (MQ=255)
                                                                         ggCTGTTTCTGCCAACTTCCGTACCGAAAGCCGTGGCGCGCATAGCCGCTTCGACTTCCCGGATCGTGATGATTAAAACTGGCTGTGCCACTCCCTGTATCTGCCAGAGTCGGAATCCATGACGCGCCGAAGCGTCAACATGGAACCGa                                                                       >  1:392685/1‑149 (MQ=255)
                                                                         gcgtGTTTCTGCCGCCTTTCGCCCCGAAATCCGTTGCGCCCATGGCCTCTTCGACTTCCCGGATCGTGATGATTAAAACTGGCTGTGCCACTCCCTGTGTCTGCCAGAGTCGGGATCCATGACGCGCCGAAGCGTCAACATGGAACCGa                                                                       <  2:515215/146‑1 (MQ=255)
                                                                           ctttttCTGCAAACTCCCGTGCCGAAAGCTGTTGTGCGCATAGACAATTCGGCGCCCCGGATCGTGATGATTAAAACTGGCTGGTCCACTCCCTGTATCTGCCAGAGTCGGAATCCATGACGCGCCGAAGCGTCAACATGGAACCGaaa                                                                     <  1:533890/146‑1 (MQ=255)
                                                                            tGTTTCTGTCAACTTCCGTACCGAAAGCCGTGGCGCGCATAGCCGCTTCGACTTCCCGGATCGTGATGATTAAAACTGGCTGTGCCACTCCCTGTATCTGCCAGAGTCGGAATCCATGACGCGCCGAAGCGTCAACATGGAACCGaaaa                                                                    >  1:347392/1‑148 (MQ=255)
                                                                                   gCCAACTTCCGTACCGAAAGCCGTGGCGCGCATAGTCGCTTCGACTTCCCGGATCGTGATGATTAAAACTGGCTGTGCCACTCCCTGTATCTGCCAGAGTCGGAATCCATGACGCGCCGAAGCGTCCACATGGAACCGAAACTGCGccc                                                             >  1:486478/1‑149 (MQ=255)
                                                                                    ccACCTTCCGGACCGAAAGCCGTGGCGCGCATAGCCGCTTCGACTTCCCGGATCGTGATGATTAAAACTGGCTGTGCCACTCCCTGTATCTGCCAGAGTCGGAATCCATGACGCGCCGAAGCGTCAACATGGAACCGAAACTGCGCCCg                                                            <  2:509788/149‑1 (MQ=255)
                                                                                      aaCTTCCGTACCGAAAGCCGTGGCGCGCATAGCCGCTTCGACTTCCCGGATCGTGATGATTAAAACTGGCTGTGCCACTCCCTGTATCTGCCAGAGCCGGAATCCATGACGCGCCGAAGCGTCAACATGGAACCGAAACTGCGCCCGGc                                                          >  1:554651/1‑149 (MQ=255)
                                                                                         ttCCGTACCGAAAGCCGTGGCGCGCATAGCCGCTTCGACTTCCCGGATCGTGATGATTAAAACTGGCTGTGCCACTCCCTGTATCTGCCAGAGTCGGAATCCATGACGCGCCGAAGCGTCAACATGGAACCGAAACTGCGCCCGGCAtt                                                       >  2:644369/1‑149 (MQ=255)
                                                                                             gTACAGAAAGCCGTGGCGCGCATAGCCGCTTCGACTTCCCGGATCGTGATGATTAAAACTGGCTGTGCCAATTCCTGTATCTGCCAGAGTCGGAATCCCTGAGGCGCCGGAGCGTCAACATGGAACAGAAAGCGCGCCCGGCATTAccg                                                   >  2:172713/1‑149 (MQ=255)
                                                                                                       ccGGGGCGCGCATGGCCGCTTCGACTTCCCGGATCGCGATGATTAAAACTGGCTGTGCCACTCCCTGTATCTGCCAGAGTCGGAATCCAGGACGCGCCGAAGCGTCAACATGGAACCGAAACTGCGCCCGGCATTCCCGCCGAAGATTc                                         <  1:62829/149‑1 (MQ=255)
                                                                                                         gTGGCGCGCAGAGCCGCTGCGACTTTGCGGATCGTGAGGAATCAAACTGGCTGTGGCGCGCCCTGTAGCGGCCAGGGTCGGAAGCCAGGAGGCGCCGAAGCGTCAACATGGAACCGAAACTGCGCCCGGCATTCCCGCCGAAGATTCGt                                       <  1:322236/149‑1 (MQ=255)
                                                                                                                 cATAGCCGCTTCGACTTCCCGGATCGTGATGATTAAAACTGGCTGGGCCACTCCCTGTATCTGCCAGAGTCGGAATCCACGACGCACCGAAGCGGCAAAATGGAACAGAAAATGCGCCCGGCATGCCCGCAGAAGATTAGtacttcata                               >  2:103386/1‑145 (MQ=255)
                                                                                                                     gCCGCTTCGACTTCCCGGATCGTGATGATTAAAACTGGCTGTGCCACTCCCTGTATCTGCCAGAGTCGGAATCCATGACGCGCCGAAGCGTCAACATGGAACCGAAACTGCGCCCGGCATTCCCGCCGAAGATTCGTACTTACTAATGc                           <  1:51888/149‑1 (MQ=255)
                                                                                                                      ccGCTTCGACTTCCCGGATCGTGATGATTAAAACTGGCTGTGCCACTCCCTGTATCTGTCAGAGTCGGAATCCATGACGCGCCGAAGCGTCAACATGGAACCGAAACTGCGCCCGGCATTCCCGCCGAAGATTCGTACTTACTAATGCg                          >  1:497593/1‑149 (MQ=255)
                                                                                                                      ccGCTTCGACTTCCCGGATCGTGATGATTAAAACTGGCTGTGCCACTCCCTGTATCTGCCAGAGTCGGAATCCATGACGCGCCGAAGCGTCAACATGGAACCGAAACTGCGCCCGGCATTCCCGCCGAAGATTCGTACTTACTAATGCg                          >  2:355368/1‑149 (MQ=255)
                                                                                                                        gCTTCGACTTCCCGGATCGTGATGATTAAAACTGGCTGTGCCACTCCCTGTATCTGCCAGAGTCGGAATCCATGACGCGCCGAAGCGTCAACATGGAACCGAAACTGCGCCCGGCATTCCCGCCGAAGATTCGTACTTACTAATGCGga                        <  1:15071/149‑1 (MQ=255)
                                                                                                                         cttcgatcccccGGAGCGCGATGATTAAAACTGGCTGTGCCACTCCCTGTATCTGCCAGAGTCGGAATCCATGACGCGCCGAAGCGTCAACATGGAACCGAAACTGCGCCCGGCATTCCCGCCGAAGATTCGTACTTACTAATGCGgag                       <  1:16274/140‑1 (MQ=255)
                                                                                                                         cTTCGACTTCCCGGATCGTGATGATTAAAACTGGCTGTGCCACTCCCTGTATCTGCCAGAGTCGGAATCCATGACGCGCCGAAGCGTCAACATGGAACCGAAACTGCGCCCGGCATTCCCGCCGAAGATTCGTACTTACTAATGCGgag                       <  1:57736/149‑1 (MQ=255)
                                                                                                                                 tCCCGGATCGTGATGATTAAAACTGGCTGTGCCACTCCCTGTATCTGCCAGAGTCGGAATCCATGACGCGCCGAAGCGTCAACATGGAACCGAAACTGCGCCCGGCATTCCCGCCGAAGATTCGTACTTACTAATGCGGAGACAGGaaa               <  2:211247/149‑1 (MQ=255)
                                                                                                                                 tCCCGGATCGTGATGATTAAAACTGGCTGTGCCACGCCCTGTATCTGCCAGACTCGGAATCCATGACGCGCCAAAGCGTCAACATGGAACCGAAACTGCGCCCGGCATTACCGCCGGAGATTAGTACTTACTAATGCggcgccaagcaa               >  2:183185/1‑139 (MQ=255)
                                                                                                                                   ccGGATCGTGATGATTAAAACTGGCTGTGCCACTCCCTGTATCTGCCAGAGTCGGAATCCATGACGCGCCGAAGCGTCAACATGGAACCGAAACTGCGCCCGGCATTCCCGCCGAAGATTCGTACTTACTAATGCGGGGACAGGAAAAt             >  1:4910/1‑149 (MQ=255)
                                                                                                                                   ccGGATCGTGATGATTAAAACTGGCTGTGCCACTCCCTGTATCTGCCAGAGTCGGAATCCATGACGCGCCGAAGCGTCAACATGGAACCGAAACTGCGCCCGGCATTCCCGCCGAAGATTCGTACTTACTAATGCGGAGACAGGAAAAt             >  2:278742/1‑149 (MQ=255)
                                                                                                                                   ccGGATCGTGATGATTAAAACTGGCTGTGCCACTCCCTGTATCTGCCAGAGTCGGAATCCATGACGCGCCGAAGCGTCAACATGGAACCGAAACTGCGCCCGGCATTCCCGCCGAAGATTCGTACTTACTAATGCGGAGACAGGAAAAt             >  1:372425/1‑149 (MQ=255)
                                                                                                                                   ccGGACCGTGATGATTAAAACTGGCTGTGCCACTCCCTGTATCTGCCAGAGTCGGAATCCATTACGCGCCGAAGCGTCAACATGGAACCGAAACTGCGCCCGGCATTCCCGCCGAAGATTCGTACTTACTAATGCGGAGACAGGAAAAt             <  1:675844/149‑1 (MQ=255)
                                                                                                                                     gggccGTGATGATCAAAACTGGCTGTGCCACTCCCTCTATCTGCCAGAGTCGGAATCCATGACGCGCCGAAGCGTCAACATGGAACCGAAACTGCGCCCGGCATTCCCGCCGAAGATTCGTACTTACTAATGCGGAGACAGGAAAATga           <  1:43626/145‑1 (MQ=255)
                                                                                                                                     cgaacGTGATCATCAAAACTCGCTCTGCCACTCCCTATATCCGCCAGAGTCGGAATCCACGACGCGCCGAAGCGTCAACATGGAACCGAAACTGCGCCCGGCATTCCCGCCGAAGATTCGTACTTACTAATGCGGAGACAGGAAAATga           <  2:390733/145‑1 (MQ=255)
                                                                                                                                          gTGATGATTAAAACTGGCTGTGCCACTCCCTGTATCTGCCAGAGTCGGAATCCATGACGCGCCGAAGCGTCAACATGGAACCGAAACTGCGCCCGGCATCCCCGCCGAAGAGTCGTACTTACTAATGCGGAGACAAGAAAATGAGCCTc      >  2:704829/1‑149 (MQ=255)
                                                                                                                                              tgattaAAACTGCCTGTGCCACCCCCTGTATCTGCCAGAGTCGGAATCCATGACGCGCCGAAGCGTCAACATGGAACCGAAACTGCGCCCGGCATTCCCGCCGAAGATTCGTACTTACTAATGCGGAGACAGGAAAATGAGACTCGAGt  <  2:82633/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                
CTTCCAGCGAGTTCAACACCCAGCGCGTTGAGTGCCTGGAACTGGATAACCTGATGGAAACGGCGTATGCAACGGCTGTTTCTGCCAACTTCCGTACCGAAAGCCGTGGCGCGCATAGCCGCTTCGACTTCCCGGATCGTGATGATGAAAACTGGCTGTGCCACTCCCTGTATCTGCCAGAGTCGGAATCCATGACGCGCCGAAGCGTCAACATGGAACCGAAACTGCGCCCGGCATTCCCGCCGAAGATTCGTACTTACTAATGCGGAGACAGGAAAATGAGACTCGAGT  >  CP009273/752867‑753157

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑