Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 717,068 G→A 9.4% A41V (GCG→GTG)  kdpE ← response regulator in two‑component regulatory system with KdpD

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273717,0680GA 9.4% 196.3 / 4.2 71A41V (GCG→GTG) kdpEresponse regulator in two‑component regulatory system with KdpD
Reads supporting (aligned to +/- strand):  ref base G (25/39);  new base A (4/3);  total (29/42)
Fisher's exact test for biased strand distribution p-value = 4.33e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01

GCGATTTTGTCGCTCTCTTCGCTGCGTGCGGAAAGCACAATCACCGGCACCGCGCTCCACTGGCGCAGGTCGCGGATAAACTCAATCCCATCACCATCGGGCAGGCCGAGATCGAGAATAATCAAATCTGGCTTACGGGTTGCCGCTTCCAGCAAGCCGCGTTGCAGCGTTTCGGCCTCAAAGACGCGCATCCCGTCGCCCTCCAGCGCCGTGCGCAGAAAGCGACGAATAGCCTGTTCATCTTCAACAATCAGAACGTTTGTCACATATCCTCATGAAATTCTTCAAGTTCA  >  CP009273/716924‑717216
                                                                                                                                                |                                                                                                                                                    
gcgATTTGGTCGCTTTCGTCGCTGCGTGCGGAAAGCACAATCGCCGGCACCGCGCTCCACTGGCGCAGGTCGCGGATAAACTCAATCCCATCACCATCGGGCAGGCCGAGATCGAGAATAATCAAATCTGGCTTACGGGTTGCCGCTTc                                                                                                                                                  <  1:323454/149‑1 (MQ=255)
  gATTTTGTCGCTCTCTTCGCTGCGTGCGGAAAGCACAATCACCGGCACCGCGCTCCACTGGCGCAGGTCGCGGATAAACTCAATCCCATCACCATCGGGCAGGCCGAGATCGAGAATAATCAAATCTGGCTTACGGGTTGCCGCTTCCa                                                                                                                                                <  2:445501/149‑1 (MQ=255)
  gATTTTGTCGCTCTCTTCGCTGCGTGCGGAAAGCACAATCACCGGCACCGCGCTCCACTGGCGCAGGTCGCGGATAAACTCAATCCCATCACCATCGGGCAGGCCGAGATCGAGAATAATCAAATATGGCTGACGGGTTGCCGCTTCCa                                                                                                                                                >  1:483762/1‑149 (MQ=255)
                ctTCGCTGCGTGCGGAAAGCACAATCACCGGCACCGCGCTCCACTGGCGCAGGTCGCGGATAAACTCAATCCCATCACCATCGGGCAGGCCGAGATCGAGAATAATCAAATCTGGCTTACGGGTTGCCGCTTCCAGCAAGCCGCGTTGc                                                                                                                                  <  1:610027/149‑1 (MQ=255)
                ctTCGCTGCGTGCGGAAACCACAATCACCGGCACCGCGCTCCACTGGCGCAGGTCGCGGATAAACTCAATCCCCTCACCATCGGGCAGGCCGAGATCGAGAATAATCAAATCTGGCTTACGGGTTGCCGCTTCCAGCAAGCCGCGTTGc                                                                                                                                  <  1:628435/149‑1 (MQ=255)
                ctTCGCCGCGTGCGGAAAGCACAATCACCGGCACCGCGCTCCACTGGCGCAGGTCGCGGATAAACTCAATCCCATCACCATCGGGCAGGCCGAGATCGAGAATAATCAAATCTGGCTTACGGGTTGCCGCTTCCAGCAAGCCGCGTTGc                                                                                                                                  <  1:91708/149‑1 (MQ=255)
                 ttCGCTGCGTGCGGAAAGCACAATCACCGGCACCGCGCTCCACTGGCGCAGGTCGCGGATAAACTCAATCCCATCACCATCGGGCAGGCCGAGATCGAGAATAATCAAATCTGGCTTACGGGTTGCCGCTTCCAGCAAGCCGCGTTGCa                                                                                                                                 <  2:708631/149‑1 (MQ=255)
                        cgtgccGACAGCAAACTCACCGGCACCGCGCCCCACTGGCGCAGGTCGCGGATAAACCCAAGCCCATCACCATCGGGCAGGCCGAGATCGAGAATAATCAAATCTGGCTTACGGGTTGCCGCTTCCAGCAAGCCGCGTTGCAGCGTTTc                                                                                                                          <  1:118199/149‑1 (MQ=255)
                           gcgGAAAGCACAATCACCGGCACCGCGCTCCACTGGCGCAGGTCGCGGATAAACTCAATCCCATCACCATCGGGCAGGCCGAGATCGAGAATAATCAAATCTGGCTTACGGGTTGCCGCTTCCAGCAAGCCGCGTTGCAGCGTTTCGGc                                                                                                                       >  1:295566/1‑149 (MQ=255)
                             ggAAAGCACAATCCCCGGCACCGCGCTCCACTGGCGCAGGTCGCGGATAAACTCAATCCCATCACCATCGGGCAGGCCGAGATCGAGAATAATCAAATCTGGCTTACGGGTTGCCGCTTCCAGCAAGCCGCGTTGCAGCGTTTCGGCCt                                                                                                                     <  2:321863/149‑1 (MQ=255)
                              gcaaGCCCAATCAGCGGCCCCGGGCTCCACTGGCGCAGGTCGCGGATAAACTAAATCCCATCACCATTGGGCAGGCCGAGATCGAGAATAATCAAATCTGGCTTACGGGTTGCCGCTTCCAGCAAGCCGCGTTGCAGCGTTTCGGCCTc                                                                                                                    <  1:88643/147‑1 (MQ=255)
                               aaaGCACAATCACCGGCACCGCGCTCCACTGGCGCAGGTCGCGGATAAACTCAATCCCATCACCATCGGGCAGGCCGAGATCGAGAATAATCAAATCTGGCTTACGGGTTGCCACTTCCAGCAAGCCGCGTTGCAGCGTTTCGGCCTCa                                                                                                                   >  2:177252/1‑149 (MQ=255)
                               aaaGCACAATCACCGGCACCGCGCTCCACTGGCGCAGGTCGCGGATAAACTCAATCCCATCACCATCGGGCAGGCCGAGATCGAGAATAATCAAATCTGGCTTACGGGTTGCCACTTCCAGCAAGCCGCGTTGCAGCGTTTAGGCCTCa                                                                                                                   >  2:157248/1‑149 (MQ=255)
                               aaaGCACAATCACCGGCACCGCGCTCCACTGGCGCAGGTCGCGGATAAACTCAATCCCATCACCATCGGGCAGGCCGAGATCGAGAATAATCAAATCTGGCTTACGGGCTGCCACTTCCAGCAAGCCGCGTTGCAGCGTTTCGGCCTCa                                                                                                                   >  2:472158/1‑149 (MQ=255)
                                 cgccaaaTCACCGGCACCGCGCTCCACTGGCGCAGGTCGCGGATAAACTCAATCCCATCACCATCGGGCAGGCCGAGATCGAGAATAATCAAATCTGGCTTACGGGTTGCCGCTTCCAGCAAGCCGCGTTGCAGCGTTTCGGCCTCaaa                                                                                                                 <  2:519822/144‑1 (MQ=255)
                                   cacaATCACCGGCACCGCGCTCCACTGGCGCAGTACGCGGATAAACTCAATCCCATCACCATCGGGCAGGCCGAGATCGGGAGTAAGCAGCACTGGCATACGGGTTGCCACTTCCAGCAAGCCGCGGTGCCGCGCTTCGGCCGCCAAgg                                                                                                               >  1:155601/1‑148 (MQ=255)
                                    acaATCACCGGCACCGCGCTCCACTGGCGCAGGTCGCGGATAAACTCAATCCCATCACCATCGGGCAGGCCGAGATCGAGAATAATCAAATCTGGCTTACGGGTTGCCGCTTCCAGCAAGCCGCGTTCCACCGGCTCGGCCTccaggcc                                                                                                              >  2:309304/1‑143 (MQ=255)
                                      aaTCACCGGCACCGCGCTCCACTGGCGCAGGTCGCGGATAAACTCAATCCCATCACCATCGGGCAGGCCGAGATCGAGAATAATCAAATCTGGCTTACGGGTTGCCGCTTCCAGCAAGCCGCGTTGCAGCGTTTCGGCCTCAAAGCcgc                                                                                                            >  2:711136/1‑149 (MQ=255)
                                      aaTCACCGGCACCGCGCTCCACTGGCGCAGGTCGCGGATAAACTCAATCCCATCACCATCGGGCAGGCCGAGATCGAGAATAATCAAATCTGGCTTACGGGTTGCCGCTTCCAGCAAGCCGCGTTGCAGCGTTTCGGCCTCAAAGAcgc                                                                                                            >  2:125503/1‑149 (MQ=255)
                                        tCACCGGCACCGCGCTCCACTGGCGCAGGTCGCGGATAAACTCAATCCCATCACCATCGGGCAGGCCGAGATCGAGAATAATCAAATCTGGCTTACGGGTTGCCGCTTCCAGCAAGCCGCGTTGCAGCGTTTCGGCCTCAAAGACgcgc                                                                                                          <  2:827780/149‑1 (MQ=255)
                                                aCCGCGCTCCACTGGCGCAGGTCGCGGATAAACTCAATCCCATCACCATCGGGCAGGCCGAGATCGAGAATAATCAAATCTGGCTTACGGGTTGCCGCTTCCAGCAAGCCGCGTTGCAGCGTTTGGGCCTCAAAGACGCGCATCCCGTc                                                                                                  >  2:195441/1‑149 (MQ=255)
                                                 ccGCGCTCCACTGGCGCAGGTCGCGGATAAACTCAATCCCATCACCATCGGGCAGGCCGAGATCGAGAATAATCAAATCTGGCTTACGGGCTGCCGCTCCCAGCAAGCCGCGTTGCAGCGTTTCGGCCTCAAAGACGCGCATCCCGTCg                                                                                                 <  2:817890/149‑1 (MQ=255)
                                                   gcgcTCCACTGGCGCAGGTCGCGGATAAACTCAATCCCATCACCATCGGGCAGGCCGAGATCGAGAATAATCAAATCTGGCTTACGGGTTGCCGCTTCCAGCAAGCCGCGTTGCAGCGTTTCGGCCTCAAAGACGCGCATCCCGTCGcc                                                                                               >  2:173897/1‑149 (MQ=255)
                                                   gcgcTCCACTGGCGCAGGTCGCGGATAAACTCAATCCCATCACCATCGGGCAGGCCGAGATCGAGAATAATCAAATCTGGCTTACGGGTTGCCGCTTCCAGCAAGCCGCGTTGCAGCGTTTCGGCCTCAAAGACGCGCATCCCGTCGcc                                                                                               >  2:746190/1‑149 (MQ=255)
                                                       tgcACTGACGCCGGTCGCGGATAAAGTCAATCCCCTCACCATCGGGCAGGCCGAGATCGAGAATAATCAAATCTGGCTTACGGGTTGCCGCTTCCAGCAAGCCGCGTTGCAGCGTTTCGGCCTCAAAGACGCGCATCCCGTCGCCCTcc                                                                                           <  1:67509/147‑1 (MQ=255)
                                                          acgggggcAGGTCGCGGATAAACCCAATCCCATCACCATCGGGCAGGCCGAGATCGAGAATAATCAAATCTGGCTTATGGGTTGCCGCTTCCAGCAAGCCGCGTTGCAGCGTTTCGGCCTCAAAGACGCGCATCCCGTCGCCCTCCAgc                                                                                        <  1:155370/143‑1 (MQ=255)
                                                          aCTGGCGCAGGTCGCGGATAAACTCAATCCCATCACCATCGGGCAGGCCGAGATCGAGAATAATCAAATCTGGCTTACGGGTTGCCGCTTCCAGCAAGCCGCGTTGCAGCGTTTCGGCCTCAAAGACGCGCATCCCGTCGCCCTCCAgc                                                                                        <  1:279288/149‑1 (MQ=255)
                                                            tGGCGCAGGTCGCGGATAAACTCAATCCCATCACCATCGGGCAGGCCGAGATCGAGAATAATCAAATCTGGCTTACGGGTTGCCGCTTCCAGCAAGCCGCGTTGCAGCGTTTCGGCCTCAAAGACGCGCATCCCGTCGCCCTCCAgcgc                                                                                      >  2:580918/1‑149 (MQ=255)
                                                            tGGCGCAGGTCGCGGATAAACTCAATCCCATCACCATCGGGCAGGCCGAGATCGAGAATAATCAAATCTGGCTTACGGGTTGCCGCTTCCAGCAAGCCGCGTTGCAGCGTTTCGGCCTCAAAGACGCGCATCCCGTCGCCCTCCAccgc                                                                                      >  1:130750/1‑149 (MQ=255)
                                                              gcgcAGGTCGCGGATAAACTCAATCCCATCACCATCGGGCAGGCCGAGATCGAGAATAATCAAATCTGGCTTACGGGTTGCCGCTTCCAGCAAGCCGCGTTGCAGCGTTTCGGCCTCAAAGACGCGCATCCCGTCGCCCTCCAGCGCCg                                                                                    <  2:298214/149‑1 (MQ=255)
                                                                  aGGTCGCGGATAACCTCAAGCCCATCACCATCGGGCAGGCCGAGATCGAGAATAATCAAATCTGGCTTACGGGTTGCCGCTTCCAGCAAGCCGCGTTGCAGCATTTCGGCCTCAAAGACGCGCATCCCGTCGCCCTCCAGCGCCGTgcg                                                                                <  2:251689/149‑1 (MQ=255)
                                                                  aGGTCGCGGATAAACTCAATCCCATCACCATCGGGCAGGCCGAGATCGAGAATAATCAAATCTGGCTTACGGGTTGCCGCTTCCAGCAAGCCGCGTTGCAGCGTTTCGGCCTCAAAGACGCGCATCCCGTCGCCCTCCAGCGCCGTgcg                                                                                <  1:587209/149‑1 (MQ=255)
                                                                  aGGTCGCGGATAAACTCAATCCCATCACCATCGGGCAGGCCGAGATCGAGAATAATCAAATCTGGCTTACGGGTTGCCGCTTCCAGCAAGCCGCGTTGCAGCGTTTCGGCCTCAAAGACGCGCATCCCGTCGCCCTCCAGCGCCGTgcg                                                                                <  2:684574/149‑1 (MQ=255)
                                                                     tCGCGGATAAACTCAATCCCATCACCATCGGGCAGGCCGAGACCGAGAATAACCAAATCTGGCTCACGGGTTGCAGCTTCCCGCAAGCCGCCTTGCAGCGTTTCGGCCGCAAAGACGCGCAGCCCGTTGCCCTCCAGCGCCGTgcgccg                                                                             >  1:723272/1‑147 (MQ=255)
                                                                          gATAAACTCAATCCCATCACCATCGGGCAGGCCGAGATCGAGAATAATCAAATCTGGCTTACGGGTTGCCGCTTCCAGCAAGCCGCGTTGCAGCGTTTCGGCCTCAAAGACGCGCATCCCGTCGCCCTCCAGCGCCGTGCGCAGAAAGc                                                                        <  2:318691/149‑1 (MQ=255)
                                                                          gATAAACTCAATCCCATCACCATCGGGCAGGCCGAGATCGAGAATAATCAAATCTGGCTTACGGGTTGCCGCTTCCAGCAAGCCGCGTTGCAGCGTTTCGGCCTCAAAGACGCGCATCCCGTCGCCCTCCAGCGCCGTGCGCAGAAAGc                                                                        <  2:67277/149‑1 (MQ=255)
                                                                           aTAAACTCAATCCCATCACCATCTGGCAGGCCGAAATCGAAAATAATCAAATCTGGCCTACGGGTTGCCGCTTCCAGCAAGCCGCGTTGCAGCGTTTCGGCCTCAAAGACGCGCATCCCGTCGCCCTCCAGCGCCGTGCGCAGAAAGcg                                                                       <  2:388581/149‑1 (MQ=255)
                                                                             aagcTCAATCCGAGCGCCTGCGGGCAGGCCGAGATCGAGAATAATCAAATCTGGCTTACGGGTTGCCGCTTCCAGCAAGCCGCGTTGCAGCGTTTCGGCCTCAAAGACGCGCATCCCGTCGCCCTCCAGCGCCGTGCGCAGAAAGcgac                                                                     <  1:797812/146‑1 (MQ=255)
                                                                              aaCTCAATCCCATCACCATCGGGCAGGCCGAGATCGAGAATAATCAAATCTGGCTTACGGGTTGCCGCTTCCAGCAAGCCGCGTTGCAGCGTTTCGGCCTCAAAGACGCGCATCCCGTCGCCCTCCAGCGCCGTGCGCAGAAAGcgacg                                                                    >  2:59881/1‑149 (MQ=255)
                                                                                  cAATCCCATCACCATCGGGCAGGCCGAGATCGAGAATAATCAAATCTGGCTTACGGGTTGCCGCTTCCAGCAAGCCGCGTTGCAGCGTTTCGGCCTCAAAGACGCGCATCCCGTCGCCCTCCAGCGCCGTGCGCAGAAAGCGACGAATa                                                                >  2:90428/1‑149 (MQ=255)
                                                                                    accccatccccatcGGGCAGGCCGAGATCGAGAATAATCAAATCTGGCTCACGGGTTGCCGCCTCCAGCAAGCCGCGTTGCAGCGTTTCGGCCTCAAAGACGCGCATCCCGTCGCCCTTCAGCGCGGTGCGCAGAAAGCGACGAATAGc                                                              <  1:596798/147‑1 (MQ=255)
                                                                                    aTCCCATCACCATCGGGCAGGCCGAGATCGAGAATAATCAAATCTGGCTTACGGGTTGCCGCTTCCAGCAAGCCGCGTTGCAGCGTTTCGGCCTCAAAGACGCGCATCCCGTCGCCCTCCAGCGCCGTGCGCAGAAAGCGACGAATAGc                                                              >  1:42223/1‑149 (MQ=255)
                                                                                                 cGGGCAGGCCGAGATCGAGAATAATCAAATCTGGCTTACGGGTTGCCGCTTCCAGCAAGCCGCGTTGCAGCGTTTCGGCCTCAAAGACGCGCATCCCGTCGCCCTCCAGCGCCGTGCGCAGAAAGCGACGAATAGCCTGTTCATCTTca                                                 <  2:376538/149‑1 (MQ=255)
                                                                                                    gCAGGCCAAGAACGAGAATAATCAAATCTGGCTTACGGGTTGCCGCTTCCAGCAAGCCGCGTTGCAGCGTTTCGGCCTCAAAGACGCGCATCCCGTCGCCCTCCAGCGCCGTGCGCAGAAAGCGACGAATAGCCTGTTCATCTTcaaca                                              >  1:536440/1‑149 (MQ=255)
                                                                                                      aGGCCGAGATTGAGAATAATCCAATCTGGCTGACGGGTTGCCGCTTCCAGCAAGCCGCGTTGCAGCGTTTCGGCCTCAAAGACGCGCATCCCGTCGCCCTCCAGCGCCATGCGCAGAAAGCGACGAATAGCCTGTTCATCTTCAACAAt                                            <  2:181361/149‑1 (MQ=255)
                                                                                                      aGGCCCAGACCGAGAATAATCAAAGCTGGCTTACGGGTTGCCGCTTCCAGCAAGCCGCGTTGCAGCGTCTCGGCCTCAAAGTCGCGCATCCCGTCGCCCTCCAGCGCCGTGCGCAGAAAGCGACGAATAGCCTGTTCATCTTCAACAAt                                            <  2:379725/149‑1 (MQ=255)
                                                                                                        gCCGAGATCGAGAATAATCAAATCTGGCTTACGGGTTGCCGCTTCCAGCAAGCCGCGTTGCAGCGTTTCGGCCTCAAAGACGCGCATCCCGTCGCCCTCCAGCGCCGTGCGCAGAAAGCGACGAATAGCCTGTTCATCTTCAACAATCa                                          >  2:467962/1‑149 (MQ=255)
                                                                                                          cGAGATCGAGAATAATCAAATCTGGCTTACGGGTTGCCGCTTCCAGCAAGCCGCGTTGCCGCGTGTCGGCCTCAAAGACGCGCATCCCGTCGCCCCCCAACGCGGCGCGCAGAAAGCGCCGAATAGCCTGTCCATCTGCGACAATCAGa                                        >  1:256684/1‑149 (MQ=255)
                                                                                                          cGAGATCGAGAATAATCAAATCTGGCTTACGGGTTGCCGCTTCCAGCAAGCCGCGTTGCAGCGTTTCGGCCTCAAAGACGCGCATCCCGTCGCCCTCCAGCGCCGTGCGCAGAAAGCGACGAATAGCCTGTTCATCTTCAACAATCAGa                                        >  2:31740/1‑149 (MQ=255)
                                                                                                          cGAGATCGAGAATAATCAAATCTGGCTTACGGGTTGCCGCTTCCAGCAAGCCGCGTTGCAGCGTTTCGGCCTCAAAGACGCGCATCCCGTCGCCCTCCAGCGCCGTGCGCAGAAAGCGACGAATAGCCTGTTCATCTTCAACAATCAGa                                        <  1:36970/149‑1 (MQ=255)
                                                                                                            agagCGAGAATAATCCAATCTGGCTTACGGGTTGCCGCTTCCAGCAAGCCGCGTTGCAGCGTTTCGGCCTCAAAGACGCGCATCCCGTCGCCCCCCAGCGCCGTGCGCAGAAAGCCACGAATAGCCTGTTCATCTTCAACAACCAGAAc                                      <  2:389867/149‑1 (MQ=255)
                                                                                                            agagCGAAAACAATCAAAGCTGGCTTACGGGTGCCCGCTTCCAGCGAGCCGCGTCTCAGCGTTTCGGCCTCAAAGCCGCGCATCCCGTCGCCCTCCAGCGCCGTGCGCAGAAAGCGACGAATAGCCTGTTCATCTTCAACAATCAGAAc                                      <  2:227337/149‑1 (MQ=255)
                                                                                                                cGAGAATAATCAAATCTGGCTTACGGGTTGCCGCTTCCAGCAAGCCGCGTTGCAGCGTTTCGGCCTCAAAGACGCGCATCCCGTCGCCCTCCAGCGCCGTGCGCAGAAAGCGACGAATAGCCTGTTCATCTTCAACAATCAGAACGttt                                  <  1:670824/149‑1 (MQ=255)
                                                                                                                 gagaATAATCAAATCCGGCTTACGGGTTGCCGCTTCCAGCAAGCCGCGTTGCAGCGTTTCGGCCTCAAAGACGCGCATCCCGTCGCCCTCCAGCGCCGTGCGCAGAAAGCGACGAATAGCCTGTTCATCTTCAACAATCAGAACGTTTg                                 <  2:483762/149‑1 (MQ=255)
                                                                                                                  agaATAATCAAATCTGGCTTACGGGTTGCCGCTTCCAGCAAGCCGCGTTGCAGCGTTTCGGCCTCAAGGACGCCAATCCCGTCGCCCTCCAGCGCCGTGGGCAGAAGCCGCCGATTAGCCTGTTCAGCTTCAACATTAAGAACGTTTGt                                >  1:701013/1‑149 (MQ=255)
                                                                                                                  agaATAATCAAATCTGGCTTACGGGTTGCCGCTTCCAGCAAGCCGCGTTGCAGCGTTTCGGCCTCAAAGACGCGCATCCCGTCGCCCTCCAGCGCCGTGCGCAGAAGGCGACGAATAGCCTGTTCGTCTTCAACAATCAGAACGTTTGt                                >  1:470531/1‑149 (MQ=255)
                                                                                                                  agaATAATCAAATCTGGCTTACGGGTTGCCGCTTCCAGCAAGCCGCGTTGCAGCGTTTCGGCCTCAAAGACGCGCATCCCGTCGCCCTCCAGCGCCGTGCGCAGAAAGCGACGAATAGCCTGTTCATCTTCAACAATCAGAACGTTTGt                                <  2:55266/149‑1 (MQ=255)
                                                                                                                       aatCAAATCTGGCTTACGGGTTGCCGCTTCCAGCAAGCCGCGTTGCAGCGTTTCGGCCTCAAAGACGCGCATCCCGTCGCCCTCCAGCGCCGTGCGCAGAAAGCGACGAATAGCCTGTTCATCTTCAACAATCAGAACGTTTGTCACat                           <  1:125503/149‑1 (MQ=255)
                                                                                                                       aatCAAATCTGGCTTACGGGTTGCCGCTTCCAGCAAGCCGCGTTGCAGCGTTTCGGCCTCAAAGACGCGCATCCCGTCGCCCTCCAGCGCCGTGCGCAGAAAGCGACGAATAGCCTGTTCATCTTCAACAATCAGAACGTTTGTCACat                           <  1:711136/149‑1 (MQ=255)
                                                                                                                        atCAAATCTGGCTTACGGGTTGCCGCTTCCAGCAAGCCGCGTTGCAGCGTTTCGGCCTCAAAGACGCGCATCCCGTCGCCCTCCAGCGCCGTGCGCAGAAAGCGACGAATAGCCTGTTCATCTTCAACAATCAGAACGTTTGTCACata                          >  2:582414/1‑149 (MQ=255)
                                                                                                                          cAAATCTGGCTTACGGGTTGCCGCTTCCAGCAAGCCGCGTTGCAGCGTTTCGGCCTCAAAGACGCGCATCCCGTCGCCCTCCAGCGCCGTGCGCAGAAAGCGACGAATAGCCTGTTCATCTTCAACAATCAGAACGTTTGTCACATATc                        <  2:788822/149‑1 (MQ=255)
                                                                                                                           aaaTCTGGCTTACGGGTTGCCGCTTCCAGCAAGCCGCGTTGCAGCGTTTCGGCCTCAAAGACGCGCATCCCGTCGCCCTCCAGCGCCGTGCGCAGAAAGCGACGAATAGCCTGTTCATCTTCAACAATCAGAACGTTTGTCACATATcc                       <  2:295566/149‑1 (MQ=255)
                                                                                                                               cTGGCTTACGGGTTGCCGCTTCCAGCAAGCCGCGTTGCAGCGTTTCGGCCTCAAAGACGCGCATCCCGTCGCCCTCCAGCGCCGTGCGCAGAAAGCGACGAATAGCCTGTTCATCTTCAACAATCAGAACGTTTGTCCCATATCCTCAt                   >  1:73982/1‑149 (MQ=255)
                                                                                                                                  gCTTACGGGTTGCCACTTCCAGCAAGCCGCGTTGCAGCGTTTCGGCCTCAAGGACGCGCATCCCGTCGCCCTCCAGCGCCGTGCGCAGAAAGCGACGAATAGCCTGTTCATCTTCAACAATCAGAACGTTTGTCACATATCCTCATGaa                <  1:472158/149‑1 (MQ=255)
                                                                                                                                  gCTTACGGGTTGCCACTTCCAGCAAGCCGCGTTGCAGCGTTTCGGCCTCAAAGACGCGCATCCCGTCGCCCTCCAGCGCCGTGCGCAGAAAGCGACGAATAGCCTGTTCATCTTCAACAATCAGAACGTTTGTCACATATCCTCATGaa                <  1:157248/149‑1 (MQ=255)
                                                                                                                                  gCTTACGGGTGGCCACTTCCAGCAAGCCGCGTTGCAGCGTTTCGGCCTCAAAGACGCGCATCCCGTCGCCCTCCAGCGCCGTGCGCAGAAAGCGACGAATAGCCTGTTCATCTTCAACAATCAGAACGTTTGTCACATATCCTCATGaa                <  1:177252/149‑1 (MQ=255)
                                                                                                                                    ttACGGGTTGCCGCTTCCAGCAACCCGCGTTGCAGCGTTTCGGCCTCAAAGACGCGCATCCCGTCGCCCTCCAGCGCCGTGCGCAGAAAGCGACGAATAGCCTGTTCATCTTCAACAATCAGAACGTTTGTCACATATCCTCATGAAAt              >  2:476785/1‑149 (MQ=255)
                                                                                                                                     tACGGGTTGCCGCTTCCAGCAAGCCGCGTTGCAGCGTTTCGGCCTCAAAGACGCGCATCCCGTCGCCCTCCAGCGCCGTGCGCAGAAAGCGACGAATAGCCTGTTCATCTTCAACAATCAGAACGTTTGTCACATATCCTCATGAAAtt             <  1:822522/149‑1 (MQ=255)
                                                                                                                                     tACCGGTTGCCGCTTCCAGCAAGCCGCGTTGCAGCGTTTCGGCCCCAAAGACGCGCATCCCGTCGCCCTCCAGCGCCGTGCGCAGAAAGCGACGAATAGCCTGTTCATCTTCAACAATGAGAACGTTTGTCACATATCCTCATGAAAtt             <  2:451372/149‑1 (MQ=255)
                                                                                                                                               cGCTTCCAGCAAGCCGCGTTGCAGCGTTTCGGCCTCAAAGACGCGCATCCCGTCGCCCTCCAGCGCCGTGCGCAGAAAGCGACGAATAGCCTGTTCATCTTCAACAATCAGAACGTTTGTCACATATCCTCATGAAATTCTTCAAGTTc   >  1:665853/1‑149 (MQ=255)
                                                                                                                                               cGCTTCCAGCAAGCCGCGTTGCAGCGTTTCGGCCTCAAAGACGCGCATCCCGTCGCCCTCCAGCGCCGTGCGCAGAAAGCGACGAATAGCCTGTTCATCTTCAACAATCAGAACGTTTGTCACATATCCTCATGAAATTCTTCAAGTTc   >  1:748940/1‑149 (MQ=255)
                                                                                                                                                gCTTCCAGCAAGCCGCGTTGCAGCGTTTCGGCCTCAAAGACGCGCATCCCGTCGCCCTCCAGCGCCGTGCGCAGAAAGCGACGAATAGCCTGTTCATCTTCAACAATCAGAACGTTTGTCACATATCCTCATGAAATTCTTCAAGTTCa  >  2:7151/1‑149 (MQ=255)
                                                                                                                                                |                                                                                                                                                    
GCGATTTTGTCGCTCTCTTCGCTGCGTGCGGAAAGCACAATCACCGGCACCGCGCTCCACTGGCGCAGGTCGCGGATAAACTCAATCCCATCACCATCGGGCAGGCCGAGATCGAGAATAATCAAATCTGGCTTACGGGTTGCCGCTTCCAGCAAGCCGCGTTGCAGCGTTTCGGCCTCAAAGACGCGCATCCCGTCGCCCTCCAGCGCCGTGCGCAGAAAGCGACGAATAGCCTGTTCATCTTCAACAATCAGAACGTTTGTCACATATCCTCATGAAATTCTTCAAGTTCA  >  CP009273/716924‑717216

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑