Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,861,917 G→A 36.1% Q66* (CAG→TAG)  nlpD ← activator of AmiC murein hydrolase activity, lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,861,9170GA36.1% 46.6 / 46.8 60Q66* (CAG→TAG) nlpDactivator of AmiC murein hydrolase activity, lipoprotein
Reads supporting (aligned to +/- strand):  ref base G (21/17);  new base A (5/16);  total (27/33)
Fisher's exact test for biased strand distribution p-value = 2.86e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.44e-01

TCGGAATGTTCCCATACTGACGGTTATAGACGATGCGTCCGTTTTCCATCTGTACCGGCTGCTGAGCTACTGGCTGCACTGGCTGGATTTGCGGTTGTTGAGTAGCCTGAATTTGTGGCTGCTGCACCGGCTGAATTTGCGGTTGCTGCGCTGTAGACGTCGTCCCCATTTTCGGCGGCGGCGTAATCAACATACCAGAATTAGTATTTGCAGGCGCATTGCCATTAACGGAGCTGACCGGTGCCGGTGGATTTGAAGTGTCAGAACAGCCTGCCAGCCATAGCGAAACCAGTGA  >  CP009273/2861770‑2862064
                                                                                                                                                   |                                                                                                                                                   
tCGGAATGTTCCCATACTGACGGTTATAGACGATGCGTCCGTTTTCCATCTGTACCGGCTGCTGAGCTACTGGCTGCACTGGCTGGATTTGCGGTTGTTGAGTAGCCTGAATTTGTGGCTGCTGTACCGGCTGAATTTGCGGTtgctgc                                                                                                                                                    <  2:644741/149‑1 (MQ=255)
 cGGAATGTTCCCTTACTGACGGTTATGGACGAGGCGTCCGTTTTCCATCTGTACCGGCTGCTGAGCTACTGGCTGCACTGGCTGGATTTGCGGTTGTTGAGTAGCCTGAATTTGTGGCTGCTGCACCGGCTGAATTTGCGGTTGCTgcg                                                                                                                                                   <  2:608858/149‑1 (MQ=255)
     aTGTTCCCATACTGACGGTTATAGACGATGCGTCCGTTTTCCATCTGTACCGGCTGCTGAGCTACTGGCTGCACTGGCTGGATTTGCGGTTGTTGAGTAGCCTGAATTTGTGGCTGCTGCACCGGCTGAATTTGCGGTTGCTGCGCTGt                                                                                                                                               <  2:855740/149‑1 (MQ=255)
      tGTTCCCATACTGACGGTTATAGACGATGCGTCCGTTTTCCATCTGTACCGGCTGCTGAGCTACTGGCTGCACCGGCTGGATTTGCGGTTGTTGAGGAGCCTGAATTTGTGGCTGCTGCACCGGCTGAATTTGCGGTTGCTGCGCTGTa                                                                                                                                              <  2:438322/149‑1 (MQ=255)
         tCCCATACTGACGGTTATAGACGATGCGTCCGTTTTCCATCTGTGCCGGCTGCTGAGCTACTGGCTGCACTGGCTGGATTTGCGGTTGTTGAGTAGCCTGAATTTGTGGCTGCTGCACCGGCTGAATTTGCGGTTGCTGCGCTGTAGAc                                                                                                                                           <  2:33978/149‑1 (MQ=255)
         tCCCATACTGACGGTTATAGACGATGCGTCCGTTTTCCATCTGTACCGGCTGCTGAGCTACTGGCTGCACTGGCTGGCTTTGCGGTTGTTGAGTAGCCTGAATTTGTGGCTGCTGCACCGGCTGAATTTGCGGTTGCTGCGCTGTAGAc                                                                                                                                           >  1:420950/1‑149 (MQ=255)
         tCCCATACTGACGGTTATAGACGATGCGTCCGTTTTCCATCTGTACCGGCTGCTGAGCTACTGGCTGCACTGGCTGGATTTGCGGTTGTTGAGTAGCCTGAATTTGTGGCTGCTGCACCGGCTGAATTTGCGGTTGCTGCGCTGTAggc                                                                                                                                           >  2:743149/1‑147 (MQ=255)
         tCCCATACTGACGGTTATAGACGATGCGTCCGTTTTCCATCTGTACCGGCTGCTGAGCTACTGGCTGCACTGGCTGGATTTGCGGTTGTTGAGTAGCCTGAATTTGTGGCTGCTGCACCGGCTGAATTTGCGGTTGCTACGCTGTAGAc                                                                                                                                           <  2:615056/149‑1 (MQ=255)
             aTACTGGCGGTTATAGACGATGCGTCCGTTTTCCATCTGTACCGGCTGCTGAGCTACTGGCTGCCCTGGCTGGATTTGCGGTTGTTGAGTAGCCTGAATTTGTGGCTGCTGCACCGGCTGAGTTTGCGGTTGCTGCGCGGTAGGcgtcg                                                                                                                                       >  1:754140/1‑149 (MQ=255)
              tACTGACGGTTATAGACGATGCGTCCGTTTTCCATCTGTACCGGCTGCTGAGCTACTGGCTGCACTGGCTGGATTTGCGGTTGTTGAGTAGCCTGAATTTGTGGCTGCTGCACCGGCTGAATTTGCGGTTGCTGCGCTGTCGAcgtcgt                                                                                                                                      >  2:351846/1‑149 (MQ=255)
              tACTGACGGTTATAGACGATGCGTCCGTTTTCCATCTGTACCGGCTGCTGAGCTACTGGCTGCACTGGCTGGATTTGCGGTTGTTGAGTAGCCTGAATTTGTGGCTGCTGCACCGGCTGAATTTGCGGTTGCTGCGCTGTAGAcgtcgt                                                                                                                                      >  2:575458/1‑149 (MQ=255)
               aCTGCCGGTTTTAGCAGTTGCGTCTGTTTTTCTTGTGTCCCGGGTGCTGCGCTACTGGGTGCACTGCGTGGATTTGGTGGTTTTGGGTGGTGTGAAGGTTTGTCGGCTTCACCGGCTGAATTTGCGGTTGCTGCGCTGTAGACgtcgtc                                                                                                                                     <  1:76357/149‑1 (MQ=255)
                cTGACGGTTATAGACGCTGCGTCCGTTTTCCATCTGTACCGGCTGCTGAGCTACTGGCTGCACTGGCTGGATTTGCGGTTGTTGAGTAGCCTGAATTTGTGGCTGCTGCACCGGCTGAATTTGCGGTTGCTGCGCTGTAGACGTCGTcc                                                                                                                                    <  1:298039/149‑1 (MQ=255)
                 tGACGGTTATAGACGATGCGTCCGTTTTCCATCTGTACCGGCTGCTGAGCTACTGGCTGCACTGGCTGGATTTGCGGTTGTTGAGTAGCCTGAATTTGTGGCTGCTGCACCGGCTGAATTTGCGGTTGCTACGCGGTACACGTCGcccc                                                                                                                                   >  1:320313/1‑149 (MQ=255)
                       ttATAGACGATGCGTCCGTTTTCCATCTGTACCGGCTGCTGAGCTACTGGCTGCACTGGCTGGATTTGCGGTTGTTGAGTAGCCTGAATTTGTGGCTGCTGCACCGGCTGAATTTGCGGTTGCTACGCTGTAGACGTCGTCCCCAtttt                                                                                                                             <  2:764120/149‑1 (MQ=255)
                       ttATAGACGATGCGTCCGTTTTCCATCTGTACCGGCTGCTGAGCTACTGGCTGCACTGGCTGGATTTGCGGTTGTTGAGTAGCCTGAATTTGTGGCTGCTGCACCGGCTGAATTTGCGGTTGCTACGCTGTAGACGTCGTCCCCAtttt                                                                                                                             <  2:168454/149‑1 (MQ=255)
                         atggACGATGCCTCCGTTTCCCATCTGTACCGTCTGCTGAGCTACTGCCTGCACTGGCTGGATGTGCGGTTGTTGAGTAGGCTGAATTTGTGGCTGCTGCACCGGCTGAATTTGCGGTTGCTGCGCTGTAGACGTCGTCCCCATTTTcg                                                                                                                           <  2:429979/146‑1 (MQ=255)
                         ataGACGATGCGTCCGTTTTCCATCTGTACCGGCTGCTGAGCTACTGGCTGCACTGGCTGGATTTGCGGTTGTTGAGTAGCCTGAATTTGTGGCTGCTGCACCGGCTGAATTTGCGGTTGCTGCGCTGTAGACGTCGTCCCCATTTTcg                                                                                                                           <  1:253788/149‑1 (MQ=255)
                           aggcGATGCGTCCGTTTTCCATCTGTACCGGCTGCTGAGCTACTGGCTGCACTGGCTGGATTTGCGGTTGTTGAGTAGCCTGAATTTGTGGCTGCTGCACCGGCTGAATTTGCGGTTGCTGCGCTGTAGACGTCGTCCCCATTTTcggc                                                                                                                         <  1:797045/146‑1 (MQ=255)
                                   cGTCCGTTTTCCATCTGTACCGGCTGCTGCGCTACTGGCTGCACTGGCTGGATTTGCGGTTGTTGAGTAGCCTGAATTTGTGGCTGCTGCACCGGCTGAATTGGCGGTTGCTACGCTGTAGGCGTCGTCACCATTCTCGgcggcggcgc                                                                                                                 >  1:136399/1‑148 (MQ=255)
                                   cGTCCGTTGTCCATCTGAACCGGCTGCTGAGCTACTGACTGCACTGGCTGGATTTGCGGTTGTTGAGTAGCCTGAATTTGTGGCTGCTGCACCGGCTGAATGTGCGGTGGCACCGCGGCAGACGTCGTCCCACTTTTCGGCGGCGGGGt                                                                                                                 >  1:556419/1‑149 (MQ=255)
                                    gTCCGTTTTCCATCTGTACCGGCTGCTGAGCTACTGGCTGCACTGGCTGGATTTGCGGTTGTTGAGTAGACTGAATTTGTGGCTGCTGCACCGGCCGAATTAGCGGTTGCTGCGCTGTAGACGTCGTCACCATTTTCGGCGGCGGCGTa                                                                                                                >  1:274392/1‑149 (MQ=255)
                                     tCCGTTTTCCATCTGTACCGGCTGCTGAGCTACTGGCTGCACTGGCTGGATTTGCGGTTGTTGAGTAGCCTGAATTTGTGGCTGCTGCACCGGCTGAATTTGCGGTTGGTGAGCTGTAGACGTGGTCCCCATTTTCGGCGGCGGCGTaa                                                                                                               >  1:14172/1‑149 (MQ=255)
                                        gTTTTCCATCTGTACCGGCTGCTGAGCTACTGGCTGCACTGGCTGGATTTGCGGTTGTTGAGTAGCCTGAATTTGTGGCTGCTGCACCGGCTGAATTTGCGGTTGCTGCGCTGTAGACGTCGTCCCCCTTTTCGGCGGCGGCGTAATCa                                                                                                            >  1:157052/1‑149 (MQ=255)
                                        gTTTTCCATCTGTACCGGCTGCTGAGCTACTGGCTGCACTGGCTGGATTTGCGGTTGATGAGTAGCCTCGTTTAGAGACGGCTGCACCGGCGGAATTTGCGGCTGCTGCGCTGTAGACCTCGTCCCCACTTTCGGCGGCGCCGTAAGCa                                                                                                            >  1:96462/1‑149 (MQ=255)
                                         ttttCCATCTGTACCGGCTGCTGAGCTACTGGCTGCACTGGCTGGATTTGCGGTTGTTGAGTAGCCTGAATTTGTGGCTGCTGCACCGGCTGAATTTGCGGTTGCTACGCTGTAGACGTCGTCCCCATTTTCGGCGGCGGCGTAATCaa                                                                                                           <  2:680173/149‑1 (MQ=255)
                                          tttCCATCTGTACCGGCTGCTGAGCTACTGGCTGCACTGGCTGGATTTGCGGTTGTTGAGTAGCCTGAATTTGTGGCTGCTGCACCGGCTGAATTTGCGGTTGCTGCGCTGTAGACGTCGTCCCCATTTTCGGCGGCGGCGTAATCAAc                                                                                                          <  2:706729/149‑1 (MQ=255)
                                              cATCTGTACCGGGTGCTGAGCTACTGGCTGCACTGGCTGGATTTGCGGTTGTTGAGTAGCCTGAATTTGTGGCTGCTGCACCGGCTGAATTTGCGGTTGCTGCGCTGTAGACGTCGTCCCCATTTTCGGCGGCGGCGTAATCAACATAc                                                                                                      <  1:769855/149‑1 (MQ=255)
                                                 cTGTACCGGCTGCTGAGCTACTGGCTGCACTGGCTGGATTTGCGGTTGTTGAGTAGCCTGAATTTGTGGCTGCTGCACCGGCTGAATTTGCGGTTGCTACGCTGTAGACGTCGTCCCCATTTTCGGCGGCGGCGTAATCAACATACCAg                                                                                                   <  2:69283/149‑1 (MQ=255)
                                                  tGTACCGGCTGCTGAGCTACTGGCTGCACTGGCTGGATTTGCGGTTGTTGAGTAGCCTGAATTTGTGGCTGCTGCACCGGCTGAATTTGCGGTTGCTGCGCTGTAGACGTCGTCCCCATTTTCGGCGGCGGCGTAATCAACATACCAGa                                                                                                  <  2:14172/149‑1 (MQ=255)
                                                           tgctgAGCTACTGGCTGCACTGGCTGGATTTGCGGTTGTTGAGTAGCCTGAATTTGTGGCTGCTGCACCGGCTGAATTTGCGGTTGCTGCGCTGTAGACGTCGTCCCCATTTTCGGCGGCGGCGTAATCAACATACCAGAATTAGTAtt                                                                                         >  2:371418/1‑149 (MQ=255)
                                                            gctgAGCTACTGGCTGCACTGGCTGGATTTGCGGTTGTTGAGTAGCCTGAATTTGTGGCTGCTGCACCGGCTGAATTTGCGGTTGCTGCGCTGTAGACGTCGTCCCCATTTTCGGCGGCGGCGTAATCAACATACCAGAAATAGTGttt                                                                                        >  1:175409/1‑149 (MQ=255)
                                                            gctgAGCTACTGGCTGCACTAGCTAGATTTGCGGTTGTTGAGTAGCCTGAATTTGTGGCTGCTGCACCGGCTGAATTTGCGGTTGCTGCGCTGTAGACGTCGTCCCCATTTTCGGCGGCGGCGTAATCAACATCCCAGAATTAGTAttt                                                                                        >  2:645038/1‑149 (MQ=255)
                                                             ctgAGCTACTGGCTGCACTGGCTGGATTTGCGGTTGTTGAGTAGCCTGAATTTGTGGCTGCTGCACCGGCTGAATTTGCGGTTGCTACGCTGTAGACGTCGTCCCCATGTTCGGCGGCGGCGTAATCAACATACCAGAATTAGTATTTg                                                                                       <  1:364541/149‑1 (MQ=255)
                                                              cgcgCTACTGGCTGCACTGGCTGGATTTGCGGTTGTTGAGTAGCCTGAATTTGTGGCTGCTGCACCGGCTGAATTTGCGGTTGCTACGCTGTAGACGTCGTCCCCATTTTCGGCGGCGGCGTAATCAACATACCAGAATTAGTATTTGc                                                                                      <  1:865932/146‑1 (MQ=255)
                                                                  cTACTGGCTGCACTGGCTGGATTTGCGGTTGTTGAGTAGCCTGAATTTGTGGCTGCTGCACCGGCTGAATTTGCGGTTGCTGCGCTGTAGACGTCGTCCCCATTTTCGGCGGCGGCGTAATCAACATACCAGAATTAGTATTTGCgggg                                                                                  >  1:241089/1‑148 (MQ=255)
                                                                    aCTGGCTGCACTGGCTGGATTTGCGGTTGTTGAGTAGCCTGAATTTGTGGCTGCTGCACCGGCTGAATTTGCGGTTGCTGCGCTGTAGACGTCGTCCCCATTTTCGGCGGCGGCGTAATCAACATACCAGAATTAGTATTTGCAGgcgc                                                                                >  1:330119/1‑149 (MQ=255)
                                                                     cTGGCTGCACTGGCTGGATTTGCGGTTGTTGAGTAGCCTGAATTTGTGGCTGCTGCACCGGCTGAATTTGCGGTTGCTACGCTGTAGACGTCGTCCCCATTTTCGGCGGCGGCGTAATCAACATACCAGAATTAGTATTTGCAGGCGCa                                                                               >  1:53921/1‑149 (MQ=255)
                                                                      tGGCTGCACTGGCTGGATTTGCGGTTGTTGAGTAGCCTGAATTTGTGGCTGCTGCACCGGCTGAATTTGCGGTTGCTACGCTGTAGACGTCGTCCCCATTTTCGGCGGCGGCGTAATCACCATACCCGTCGTCGTATTTGCCGGCGCAt                                                                              >  1:774600/1‑149 (MQ=255)
                                                                         cTGCACTGCCTGCATTTGCGGCTGTTGCGTAGCCTGAATTTGTCGCTGCTGCACCCGCTGAATTTGCGGTTGCTGCGCTGTAGACGTCGTCCCCATTTTCGGCGGCGGCGTAATCAACATACCAGAATTAGTATTTGCAGGCGCATTGc                                                                           <  1:760011/149‑1 (MQ=255)
                                                                               tggctggATTTGCGGTTGTTGAGTAGCCTGAATTTGTGGCTGCTGCACCGGCTGAATTTGCGGTTGCTGCGCTGTAGACGTCGTCCCCATTTTCGGCGGCGGCGTAATCAACATACCAGAATTTGTATTTGCAGGCGCATTGCCATTaa                                                                     >  1:44952/1‑149 (MQ=255)
                                                                                 gctggATTTGCGGTTGTTGAGTAGCCTGAATTTGTGGCTGCTGCACCGGCTGAATTTGCGGTTGCTGCGCTGTAGACGTCGTCCCCATTTTCGGCGGCGGCGTAATCAACATACCAGAATTAGTATTTGCAGGCGCATTGCCATTAACg                                                                   <  2:660301/149‑1 (MQ=255)
                                                                                            ggTTGTTGAGTAGCCTGAATTTGTGGCTGCTGCACCGGCTGAATTTGCGGTTGCTACGCTGTAGACGTCGTCCCCATTTTCGGCGGCGGCGTAATCAACATACCAGAATTAGTATTTGCAGGCGCATTGCCATTAACGGAGCTGAccgg                                                        <  2:611476/149‑1 (MQ=255)
                                                                                            ggTTGTTGAGTAGCCTCAATTTGTGGCTGCTGCACCGGCTGAATTTGCGGTTGCTACGCTGTAGACGTCGTCCCCATTTTCGGCGGCGGCGTAATCAACATACCAGAATTAGTATTTGCAGGCGCATTGCCATTAACGGAGCTGAccgg                                                        <  1:428280/149‑1 (MQ=255)
                                                                                                 ttgAGTAGCCTGAATTTGTGGCTGCTGCACCGGCTGAATTTGCGGTTGCTGCGCTGTAGACGTCGTCCCCATTTTCGGCGGCGGCGTAATCAACATACCAGAATTAGTATTTGCAGGCGCATTGCCATTAACGGAGCTGAccggtgccg                                                   <  2:353103/149‑1 (MQ=255)
                                                                                                  tgAGTAGCCTGAATTTGTGGCTGCTGCACCGGCTGAATTTGCGGTTGCTGCGCTGTAGACGTCGTCCCCATTTTCGGCGGCGGCGTAATCAACATACCAGAATTAGTATTGGCAGGCGCATTGCCATTAACGGAGCTGAccgttgcggg                                                  >  1:451444/1‑149 (MQ=255)
                                                                                                  tgAGGAGCCTGAATTTGTGGCTGCTGCACCGGCTGAATTTGCGGTTGCTGCGCTGTAGACGTCGTCCCCATTTTCGGCGGCGGCGTAATCAACATACCAGAATTAGTATTTGCAGGCGCATTGCCATTAACGGAGCTGAccggtgccgg                                                  <  2:175700/149‑1 (MQ=255)
                                                                                                    aGTAGCCTGAATTTGTGGCTGCTGCACCGGCTGAATTTGCGGTTGCTGCGCTGTAGACGTCGTCCCCATTTTCGGCGGCGGCGTAATCAACATACCAGAATTAGTATTTGCAGGCGCATTGCCATTAACGGAGCTGAccggtgccggtg                                                >  2:560319/1‑149 (MQ=255)
                                                                                                    aGTAGCCTGAATTTGTGGCTGCTGCACCGGCTGAATTTGCGGTTGCTACGCTGTAGACGTCGTCCCCATTTTCGGCGGCGGCGTAATCAACATACCAGAATTAGTATTTGCAGGCGCATTGGCCAAAGCGGAGCTGAccggtgccggtg                                                >  1:650091/1‑149 (MQ=255)
                                                                                                        gCCTGAATTTGTGGCTGCTGCACCGGCTGAATTTGCGGTTGCTGCGCTGTAGACGTCGTCCCCATTTTCGGCGGCGGCGTAATCAACATACCAGAATTAGTATTTGCAGGCGCATTGCCATTAACGGAGCTGACCGGTGCCGGTGGAtt                                            >  2:422669/1‑149 (MQ=255)
                                                                                                         ccTGAATTTGTGGCTGCTGCACCGGCTGAATTTGCGGTTGCTGCGCTGTAGACGTCGTCCCCATTTTCGGCGGCGGCGTAATCAACATACCAGAATTAGTATTTGCAGGCGCATTGCCATTAACGGAGCTGACCGGTGCCGGTGGAttt                                           <  1:862534/149‑1 (MQ=255)
                                                                                                           tGAATTTGTGGCTGCTGCACCGGCTGAATTTGCGGTTGCTGCGCTGTAGACGTCGTCCCCATTTTCGGCGGCGGCGTAATCAACATACCAGAATTAGTATTTGCAGGCGCATTGCCATTAACGGAGCTGACCGGTGCCGGTGGATTTGa                                         >  2:491819/1‑149 (MQ=255)
                                                                                                            gAATTTGTGGCTGCTGCACCGGCTGAATTTGTGGTTGCTGCGCTGTAGACGTCGTCCCCATTTTCGGCGGCGGCGTAATCAACATACCAGAATTAGTATTTGCAGGCGCATTGCCATTAACGGAGCTGACCGGTGCCGGTGGATTTGaa                                        <  2:274392/149‑1 (MQ=255)
                                                                                                             aaTTTGTGGCTGCTGCACCGGCTGAATTTGCGGTTGCTACGCTGTAGACGTCGCCCCCATTTTCGGCGGCGGCGTAATCAACATACCAGAATTAGTATTTGCAGGCGCATTGCCATTAACGGAGCTGACCGGTGCCGGTGGATTTGacg                                       <  1:662636/149‑3 (MQ=255)
                                                                                                                  ggggCTGCGTCACCGGCTGTATTTGCGGTTGCTACGCTGTAGACGTCGTCCCCATTTTCGGCGGCGGCGTAATCAACATACCAGAATTAGTATTTGCAGGCGCATTGCCATTAACGGAGCTGACCGGTGCCGGTGGATTTGAAGTGTCa                                  <  1:474860/147‑1 (MQ=255)
                                                                                                                   tgtctgctgCCCCGGCTGACTTTGCGGTTGCTACGGTCTATACGTCGTCCCCATTTTCGGCGGCGGCGTAATCAACATACCAGAATTAGTATTTGCAGGCGCATTGCCATTAACGGAGCTGACCGGTGCCGGTGGATTTGAAGTGTCAg                                 <  1:774847/146‑1 (MQ=255)
                                                                                                                   tgGCTGCTGCACCGGCTGAATTTGCGGTTGCTACGCTGTAGACGTCGTCCCCATTTTCGGCGGCGGCGTAATCAACATACCAGAATTAGTATTTGCAGGCGCATTGCCATTAACGGAGCTGACCGGTGCCGGTGGATTTGAAGTGTCAg                                 <  1:761719/149‑1 (MQ=255)
                                                                                                                    ggCTGCTGCACCGGCTAAATTTGCGGTTGCTGCGCTGTAGACGTCGTCCCCATTTTCGGCGGCGGCGTAATCAACATACCAGAATTAGTATTTGCAGGCGCATTGCCATTAACGGAGCTGACCGGTGCCGGTGGATTTGAAGTGTCAGa                                >  2:544469/1‑149 (MQ=255)
                                                                                                                           gcACCGGCTGAATTTGCGGTTGCTACGCTGTAGACGTCGTCCCCATTTTCGGCGGCGGCGTAATCAACATACCAGAATTAGTATTTGCAGGCGCATTGCCATTAACGGAGCTGACCGGTGCCGGTGGATTTGAAGTGTCAGAACAGCCt                         <  1:31586/149‑1 (MQ=255)
                                                                                                                           ccACCGTCTGAATTTGCGGTTGCTACGCTGTAGACGTCGTCCCCATTTTCGGCGGCGGCGTAATCAACATACCAGAATTAGTATTTGCAGGCGCATTGCCATTAACGGCGCCTACCGGTGCCGGTGGATTTGAAGTGTCAGAACAGCCt                         <  1:365625/148‑1 (MQ=255)
                                                                                                                              ccGGCTGAATTTGCGGTTGCTACGCTGTAGACGTCGTCCCCATTTTCGGCGGCGGCGTAATCAACATACCAGAATTAGTATTTGCAGGCGCATTGCCATTAACGGAGCTGACCGGTGCCGGTGGATTTGAAGTGTCAGAACAGCCTgcc                      <  2:754140/149‑1 (MQ=255)
                                                                                                                                     aaTTTGCGGTTGCTGCGCTGTAGACGTCGTCCCCATTTTCGGCGGCGGCGTAATCAACATACCAGAATTAGTATTTGCAGGCGCATTGCCATTAACGGAGCTGACCGGTGCCGGTGGATTTGAAGTGTCAGAACAGCCTGCCAGCCATa               >  1:721340/1‑149 (MQ=255)
                                                                                                                                                  tgcGCTGTAGACGTCGTCCCCATTTTCGGCGGCGGCGTAATCAACATACCAGAATTAGTATTTGCAGGCGCATTGCCATTAACGGAGCTGACCGGTGCCGGTGGATTTGAAGTGTCAGAACAGCCTGCCAGCCATAGCGAAACCAGTGa  >  2:290264/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
TCGGAATGTTCCCATACTGACGGTTATAGACGATGCGTCCGTTTTCCATCTGTACCGGCTGCTGAGCTACTGGCTGCACTGGCTGGATTTGCGGTTGTTGAGTAGCCTGAATTTGTGGCTGCTGCACCGGCTGAATTTGCGGTTGCTGCGCTGTAGACGTCGTCCCCATTTTCGGCGGCGGCGTAATCAACATACCAGAATTAGTATTTGCAGGCGCATTGCCATTAACGGAGCTGACCGGTGCCGGTGGATTTGAAGTGTCAGAACAGCCTGCCAGCCATAGCGAAACCAGTGA  >  CP009273/2861770‑2862064

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: