Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,592,650 A→T 5.8% I157N (ATT→AAT)  dapA ← dihydrodipicolinate synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,592,6500AT 5.8% 210.1 / 3.3 69I157N (ATT→AAT) dapAdihydrodipicolinate synthase
Reads supporting (aligned to +/- strand):  ref base A (38/27);  new base T (2/2);  total (40/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.73e-01

AACGGAAATAACCCCATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAATTTGCGGCAGGTCAGTATGCTCAGCGATGGCTTTGAAATGCTGATACAAACCTTCTTGCGACGGAC  >  CP009273/2592505‑2592794
                                                                                                                                                 |                                                                                                                                                
aaCGGAAATAACCCCATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCCCTTTGATTCCGATAatat                                                                                                                                               >  1:46461/1‑149 (MQ=255)
   ggAAATAACCCCATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATAtttt                                                                                                                                            <  2:309720/149‑1 (MQ=255)
        tAACCCCATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACt                                                                                                                                       <  2:344546/149‑1 (MQ=255)
                      cccAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGcc                                                                                                                         <  2:133015/149‑1 (MQ=255)
                       ccAATTGCATGAAGTCCAGCGCGCGCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGccc                                                                                                                        <  2:439072/149‑1 (MQ=255)
                          aTTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCAcc                                                                                                                     <  2:474859/149‑1 (MQ=255)
                          aTTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCAcc                                                                                                                     <  2:716020/149‑1 (MQ=255)
                              cATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGCCGGCCCACCGttt                                                                                                                 >  2:33087/1‑149 (MQ=255)
                              cATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCCCCGttt                                                                                                                 >  2:33071/1‑149 (MQ=255)
                               aTGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTc                                                                                                                >  2:768265/1‑149 (MQ=255)
                                  aaGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCgg                                                                                                             <  2:509411/149‑1 (MQ=255)
                                    gTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAAAATATTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGa                                                                                                           <  2:425869/149‑1 (MQ=255)
                                     tCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAg                                                                                                          <  1:38812/149‑1 (MQ=255)
                                       cAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTCGACGCGCGTTAACAACCCTTTTGCCTCTTTGATTCCGATAATATTTTTTGCTTTCGCCAGACTGCAAACCGTTTCCGTGaaac                                                                                                        >  2:853750/1‑146 (MQ=255)
                                           gcccTCGCATCAGCGCCGCTCAGCAGAACAAAATCATCTGAAACCGGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATc                                                                                                    <  1:269115/146‑1 (MQ=255)
                                            cgcTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATATTATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCg                                                                                                   >  1:124426/1‑149 (MQ=255)
                                            cgcTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATATTATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCg                                                                                                   >  1:241386/1‑149 (MQ=255)
                                               tCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCAg                                                                                                >  2:234769/1‑149 (MQ=255)
                                               tCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCAg                                                                                                >  2:535812/1‑149 (MQ=255)
                                               tCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGACCACGGTTTCCTGGAGCAGAACGccg                                                                                                >  2:187324/1‑147 (MQ=255)
                                                cGCATCAGCGCCGCTCAGCAGAACAAAACCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCAGc                                                                                               <  1:504549/149‑1 (MQ=255)
                                                  catcatCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCAGCCa                                                                                             <  1:644508/149‑1 (MQ=255)
                                                    tcatcGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGt                                                                                           >  2:796945/1‑149 (MQ=255)
                                                          ccgcTCAGCGGAACAAAAGCATCTGAAACCAGGTCTTTGGTCTGGTTTCCACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGGTGATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGa                                                                                     <  1:454395/149‑1 (MQ=255)
                                                          ccgcTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGAAGGATCGCCTTTTCCGGGAGCAGATCGCAGCCAGTACGGGa                                                                                     <  2:858552/149‑1 (MQ=255)
                                                            gcTCAGTGGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACg                                                                                   <  1:729762/149‑1 (MQ=255)
                                                                          aaTCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTCTACAGa                                                                     >  2:29486/1‑149 (MQ=255)
                                                                              atcTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGCCGGCACCTTATCCAGAAttt                                                                 >  2:490797/1‑149 (MQ=255)
                                                                                  gaaacaggtcctTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATGCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAATTTGCgg                                                             >  1:149497/11‑149 (MQ=255)
                                                                                  gAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAATTTGCgg                                                             <  1:768605/149‑1 (MQ=255)
                                                                                   aaaCCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTCCGGTGCGGCACATTATACAGAATTTGCGGc                                                            >  2:682682/1‑149 (MQ=255)
                                                                                     aCCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAATTTGCGGCAg                                                          >  2:136650/1‑149 (MQ=255)
                                                                                     aCCAGCTCTTTGAACTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGGGCAGATCGCAGCCAGTACGGGACGGCACATTATACCGAATTTGCGGCAg                                                          <  2:622260/149‑1 (MQ=255)
                                                                                      ccAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAATTTGCGGCAgg                                                         <  2:156068/149‑1 (MQ=255)
                                                                                        tgCTCTTTGACTTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAATTTGCGGCAGGTc                                                       <  2:684057/148‑1 (MQ=255)
                                                                                           tctTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAATTTGCGGCAGGTCAGt                                                    <  2:442548/149‑1 (MQ=255)
                                                                                             tttGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGCATTTGCGGCAGGTCAGTAt                                                  >  2:107954/1‑149 (MQ=255)
                                                                                              ctGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAATTTGCGGCAGGTCAGTATg                                                 <  1:635898/148‑1 (MQ=255)
                                                                                                gATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCAGCCTGTACGGGACGGCACATTATACAGAATTGGCGGCAGGTCAGTATGCt                                               >  1:830830/1‑149 (MQ=255)
                                                                                                 aTCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACCTTATACAGAATTTGCGGCAGGTCAGTATGCTc                                              >  2:715842/1‑149 (MQ=255)
                                                                                                    tGGTTTACACGCGTTAAGTCCCCTGTTGCCTCGTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAATTTGCGGCAGGTCAGTATGCTCAGc                                           <  1:151475/149‑1 (MQ=255)
                                                                                                     ggTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAATTTGCGGCAGGTCAGTATGCTCAGCg                                          >  1:266862/1‑149 (MQ=255)
                                                                                                     ggTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAATTTGCGGCAGGTCAGTATGCTCAGCg                                          >  1:266858/1‑149 (MQ=255)
                                                                                                           cacGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATATTATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCAGCGAGTACGGGACGGCACATTATACAGAATTTGCGGCAGGTCAGTATGCTCAGCGATGGCt                                    <  2:124426/149‑1 (MQ=255)
                                                                                                           cacGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATATTATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAATTTGCGGCAGGTCAGTATGCTCAGCGATGGCt                                    <  2:241386/149‑1 (MQ=255)
                                                                                                            acGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGCCGGCGCAACGGGGCCGGGAGCAGATCGCGGCCAGTACGGGACGGCACATTATACAGAATTTGCGGCAGGTCAGTATGCTCAGCGATGGCtt                                   >  2:166774/1‑149 (MQ=255)
                                                                                                            acGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAATTTGCGGCAGGTCAGTATGCTCAGCGATGGCtt                                   >  1:846009/1‑149 (MQ=255)
                                                                                                             cgtgTTAAGCTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAATTTGCGGCAGGTCAGTATGCTCAGCGATGGCttt                                  <  1:169580/146‑1 (MQ=255)
                                                                                                              gcgTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAATTTGCGGCAGGTCAGTATGCTCCGCGATGGCTTTg                                 >  1:268539/1‑149 (MQ=255)
                                                                                                              gcgTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAATTTGCGGCAGGTCAGTATGCTCAGCGATGGCTTTg                                 <  1:295842/149‑1 (MQ=255)
                                                                                                              gcgTTAAGATCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGGAGGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTCAGGGCCGGCACCTTATACAGAGTTTGGGGGGGGCCAGTGTGCCCAGCCGTGGCATTg                                 >  1:210041/1‑149 (MQ=255)
                                                                                                                      ttCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACGGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAATTTGCGGCAGGTCAGGATGCTCAGCGACGGCTGTGAAATGCTg                         >  1:564772/1‑149 (MQ=255)
                                                                                                                      ttCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAATTTGCGGCAGGTCAGTATGCTCAGCGATGGCTTTGAAATGCTg                         >  2:459344/1‑149 (MQ=255)
                                                                                                                      ttCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAATTTGCGGCAGGTCAGTATGCTCAGCGATGGCTTTGAAATGCTg                         >  2:141414/1‑149 (MQ=255)
                                                                                                                      ttCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAATTTGCGGCAGGTCAGTATGCTCAGCGATGGCTTTGAAATGCTg                         <  1:609848/149‑1 (MQ=255)
                                                                                                                       tCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAATTTGCGGCAGGTCAGTATGCTCAGCGATGGCTTTGAAATGCTGa                        <  2:728264/149‑1 (MQ=255)
                                                                                                                         ccTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAATTTGCGGCAGGTCCGTATGCTCAGCGCGGGCTTTGAGATGCTGAtc                      >  1:5701/1‑148 (MQ=255)
                                                                                                                             ttGCCTCTTTGATTCCGATAATATTTTTTTCTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGCACGGCACGGCACAGTATACAGGATTGGCGGCAGGTCAGTATGCACAGCGAGTGCTTTTAAATGCTGCTACaaa                  <  1:681848/149‑1 (MQ=255)
                                                                                                                                ccTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAATTTGCGGCAGGTCAGTATGCTCAGCGATGGCTTTGAAATGCTGATACAAAcca               >  1:197015/1‑148 (MQ=255)
                                                                                                                                  tctTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAATTTGCGGCAGGTCAGTATGCTCAGCGATGGCTTTGAAATGCTGATACAAACcttc             >  2:517577/1‑149 (MQ=255)
                                                                                                                                  tctTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAATTTGCGGCAGGTCAGGATGCTCAGCGATGGCTTTGAAACGCGGATACAAACcttc             >  1:564899/1‑149 (MQ=255)
                                                                                                                                   ctTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACTTTATACAGAATTTGCGGCAGGTCAGTATGCTCAGCGATGGCTTTGAAATGCTGATACAAACcttct            >  1:849851/1‑149 (MQ=255)
                                                                                                                                          tCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCGCATTATACAGAATTTGCGGCAGGTCAGTATGCTCAGCGATGGCTTTGAAATGCTGATACAAACCTTCTTGCgacg     >  1:571411/1‑149 (MQ=255)
                                                                                                                                          tCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACGTTATACAGCATTTGCGGCAGGTCAGTGTGCTCAGCGATGGCTTTGAAATGCTGATACAAACCTTCTTGCgacg     >  1:525942/1‑149 (MQ=255)
                                                                                                                                          tCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCAGACAGTACGCGACGGCACATTATACAGCATTTGCGGCAGGTCAGTATGCGCAGCGCTGGCTTTGAAATGCTGATACAAACCTTCTTGCgccg     >  1:344718/1‑149 (MQ=255)
                                                                                                                                           ccGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTGCGGGACGGCACATTATACAGAATTTGCGGCAGGTCAGTATGCTCAGCGATGGCTTTGAAATGCTGATACAAACCTTCTTGCgacgg    >  1:653069/1‑149 (MQ=255)
                                                                                                                                            cGATAATATTTTTTTTTTTTTTTAAACGGCCCACCGGTTCCGGGAGCAGATCGCAGCCAGTAGGGGACGGCACATTATACAGAATTTGCGGCAGGACAGTATTTTCTTCTATTGCTTTGAAATGCTGATACAAACCTTCTTGGgacgga   >  1:128131/1‑149 (MQ=255)
                                                                                                                                            cGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAATTTGCGGCAGGTCAGTATGCTCAGCGATGGCTTTGAAATGCTGATACAAACCTTCTTGCgacgga   >  2:602052/1‑149 (MQ=255)
                                                                                                                                             gATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGTAGCCAGTACGGGACGGCAAATTATACAGAATTTGCGGCAGGTCAGTATGCTCAGCGATGGCTTTGCAATGCTGATACAAACCTTCTTGAgagggac  >  1:810704/1‑149 (MQ=255)
                                                                                                                                             gATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAATTTGCGGCAGGTCAGTATGCCCAGCGATGGCTTTGAAATGCTGATACAAACCTTCTTGcgaccgac  >  1:735286/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
AACGGAAATAACCCCATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAATTTGCGGCAGGTCAGTATGCTCAGCGATGGCTTTGAAATGCTGATACAAACCTTCTTGCGACGGAC  >  CP009273/2592505‑2592794

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑