Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,032,559 C→T 9.4% R119W (CGG→TGG)  hyaF → protein involved in nickel incorporation into hydrogenase‑1 proteins

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,032,5590CT 9.4% 106.1 / 2.5 43R119W (CGG→TGG) hyaFprotein involved in nickel incorporation into hydrogenase‑1 proteins
Reads supporting (aligned to +/- strand):  ref base C (21/18);  new base T (2/2);  total (23/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.61e-01

TGCTGGCAGTCGATCTGTTAAATCTCAACGCCGACGATCGTCACTTTATCAATACGCTTCTCGGGGAAGGGGAAGTGTCAGTGCGCATTCAGCAGGCTGACGACAGTGAAAGTGAAATACAGGAGGCGATCTTCTGCGGATTATGGCGGGTGCGCAGACGTCGCGGCGAAAAGTTGCTGGAGGACAAACTGGAGGCTGGCTGCGCGCCGCTGGCGTTGTGGCAGGCGGCAACGCAAAATCTCTTGCCGACAGATTCGCTGTTACCGCCGCCCATTGATGGCCTGATGAATGGCC  >  CP009273/1032413‑1032706
                                                                                                                                                  |                                                                                                                                                   
tGCTGGCAGTCGATCTGTTAAATCTCAACGCCGACGATCGTCACTTTATCAATACGCTTCTCGGGGAAGGGGAAGTGTCAGTGCGCATTCAGCAGGCTGACGACAGTGAAAGTGAAATACAGGAGGCGATCTTCTGCGGATTATGGCgg                                                                                                                                                   <  2:462840/149‑1 (MQ=255)
     gCAGTCGATCTGTTAAATCTCAACGCCGACGATCGTCACTTTACCAATACGCTTCTCGGGGAAGGGGAAGTGGGAGTGCGCATTCATCGTGCTGACGACAGTGAAAGTGAAATACAGGAGGCGATCTTCTGCGGATTATGGCGGGTgcg                                                                                                                                              <  2:205946/149‑1 (MQ=255)
                    aaTCTCAACGCCGACGATCGTCACTTTATCAATACGCTTCTCGGGGAAGGGGAAGTGTCAGTGCGCATTCAGCAGGCTGACGACAGTGAAAGTGAAATACAGGAGGCGATCTTCTGCGGATTATGGCGGGTGCGCAGACGTCGCGGCGa                                                                                                                               <  1:495353/149‑1 (MQ=255)
                      tgtcAACGCCGACGATCGTCACTTTATCAACGCGCTTCTCGGGGAAGGGGAAGTGTCAGTGCGCATTCAGCAGGCTGACGACAGTGAAAGTGGAATGCAGGAGGCGATCTTCTGCGGATTATGGCGGGTGCGCAGACGTCGCGGCGaaa                                                                                                                             <  1:665034/147‑1 (MQ=255)
                         cAACGCCGACGATCGTCACTTTATCAATACGCTTCTCGGGGAAGGGGAAGTGTCAGTGCGCATTCAGCAGGCTGACGACAGTGAAAGTGAAATACAGGAGGCGATCTTCTGCGGATTATGGCGGGTGCGCAGGCGTCGCGGCGACAAGt                                                                                                                          >  1:415843/1‑149 (MQ=255)
                         cAACGCCGACGATCGTCACATTATCAATACGCTTCACGGGGAAAGGGAACTGTCAGAGCGAAAACAGCAGGCTAACGACAGTGAAAGTGAAAAACAGTAAGCAATCAACTGCGGATAATGGCGGGTGCGCAGACGTCGCGACGAATAga                                                                                                                          >  1:571001/1‑148 (MQ=255)
                          aaCGCCGACGATCGTCACTTTATCAATACGCTTCTCGGGGAAGGGGAAGTGTCAGTGCGCATTCAGCAGGCTGACGACAGTGAAAGTGAAATACAGGAGGCGATCTTCTGCGGATTATGGCGGGTGGGCAGACGTCGCGGGGGAAAGtt                                                                                                                         >  1:50425/1‑149 (MQ=255)
                              ctggggtccGTGACTTTTTTGATTCGCTTGTCGGGGAGGGGGAAGTGTCAGTGCGCATTCAGGAGGCTGACGACAGTGAAAGTGAAATACAGGGGGCGATCTTCTGCGGATTATGGCGGGTGCGCAGACGTCGCGGTGAAAAGTTGCTg                                                                                                                     <  1:343559/141‑1 (MQ=255)
                                gacgaTCGTCACTTTATCAATACGCTTCTCGGGGAAGGGGAAGTGTCAGTGCGCATTCAGCAGGCTGACGACAGTGAAAGTGAAATACAGGAGGCGATCTTCTGGGGATTATGGCGGGTGCGGAGACGTCGGCGCGGAAAGTtggtggg                                                                                                                   >  1:343775/1‑148 (MQ=255)
                                gacgaTCGTCACTTTATCAATACGCTTCTCGGGGAAGGGGAAGTGTCAGTGCGCATTCAGCAGGCTGACGACAGTGAAAGTGAAATACAGGAGGCGATCTTCTGCGGATTCTGGCGGGTGCGCAGACGGCGCGGCGAAAAGTTGCTgga                                                                                                                   >  2:702979/1‑149 (MQ=255)
                                gacgaTCGTCACTTTATCAATACGCTTCTCGGGGAAGGGGAAGTGTCAGTGCGCATTCAGCAGGCTGACGACAGTGAAAGTGAAATACAGGAGGCGATCTTCTGCGGATGATGGCGGGTGCGGCGAAGTCGCGGCGAAAAGTTGCTgta                                                                                                                   >  2:173434/1‑147 (MQ=255)
                                           cTTTATCAATACGCTTCTCGGGGAAGGGGAAGTGTCAGTGCGCATTCAGCAGGCTGACGACAGTGAAAGTGAAATACAGGAGGCGATCTTCTGCGGATTATGGCGGGTGCGCAGACGTCGCGGCGAAAAGTTGCTGGAGGACAAACTgg                                                                                                        <  2:742867/149‑1 (MQ=255)
                                           cTTTATCAATACGCTTCTCGGGGAAGGGGAAGTGTCAGTGCGCATTCAGCAGGCTGACGACAGTGAAAGTGAAATACAGGAGGCGATCTTCTGCGGATTATGGCGGGGGCGCAGACGTCGCGGCGAAAAGTTGCTGGAGGACAAACTgg                                                                                                        >  2:684896/1‑149 (MQ=255)
                                               aTCAATACGCTTCTCGGGGAAGGGGAAGTGTCAGTGCGCATTCAGCAGGCTGACGACAGTGAAAGTGAAATACAGGAGGCGATCTTCTGCGGATTATGGCGGGTGCGCAGACGTCGCGGCGAAAAGTTGCTGGAGGACAAACTGGAggc                                                                                                    <  2:293776/149‑1 (MQ=255)
                                                  aatccgcgcctcGGGGAAGGGGAAGTGTCAGTGCGGATTCCGCAGGCTGAAGACAGTGAAACTGAAATACAGGAGGCGATCTTCTGCGGATTATGGCGGGTGCGCAGACGTCGCGGCGAAAAGTTGCTGGAGGACAAACTGGAggctgg                                                                                                 <  1:421895/140‑1 (MQ=255)
                                                     aCGCTTCTCGGGTAAGGGGAAGTTTCAGTGCTCCTTCAGCAGGCTGACGACAGTGAAAGTGAAATACAGGAGGCGATCTTCTGCGGATTATGGCGGGTGCGCAGACGTCGCGGCGAAAAGTTGCTGGAGGACAAACTGGAGgctggctg                                                                                              <  2:688367/149‑1 (MQ=255)
                                                     aCGCTTCTCGGGGAAGGGGAAGTGTCAGTGCGCATTCAGCAGGCTGACGACAGTGAAAGTGAAATACAGGAGGCGATCTTCTGCGGATTATGGCGGGTGCGCAGACGTCGCGGCGAAAAGTTGCTGGAGGACAAACTGGAGgctggctg                                                                                              <  2:777727/149‑1 (MQ=255)
                                                                      ggaagTGCCAGTGCGCATTCAGCAGGCTGACGACAGTGAAAGTGAAATACAGGAGGCGATCTTCTGCGGATTATGGCGGGTGCGCAGACGTCGCGGCGAAAAGTTGCTGGAGGACAAACTGGAGGCTGGCTGCGCGCCGCTGGCGTtgt                                                                             <  2:311892/149‑1 (MQ=255)
                                                                        aagTGTCAGTGCGCATTCAGCAGGCTGACGACAGTGAAAGTGAAATACAGGAGGCGATCTTCTGCGGATTATGGTGGGTGCGCAGACGTCGCGGCGAAAAGTTGCTGGAGGACAAACTGGAGGCTGGCTGCGCGCCGCTGGCGTTGTgg                                                                           >  1:48644/1‑149 (MQ=255)
                                                                        aagTGTCAGTGCGCATTCAGCAGGCTGACGACAGTGAAAGTGAAATACAGGAGGCGATCTTCTGCGGATTATGGTGGGTGCGCAGACGTCGCGGCGAAAAGTTGCTGGAGGACAAACTGGAGGCTGGCTGCGCGCCGCTGGCGTTGTgg                                                                           >  1:48560/1‑149 (MQ=255)
                                                                            gtCAGTGCGCATTCAGCAGGCTGAAGACAGTGAAAGTGAAATACAGGAGGCGATCTTCTGCGGATTATGGCGGGTGCGCAGACGTCGCGGCGAAAAGTTGCTGGAGGACAAACTGGAGGCTGGCTGCGCGCCGCTGGCGTTGTGGCAgg                                                                       >  2:440721/1‑149 (MQ=255)
                                                                                          agcagGCTGACGACAGTGAAAGTGAAATACAGGAGGCGATCTTCTGCGGATTATGGCGGGTGCGCAGACGTAGCGGCGAAAAGCCGCTAGAGGACCCACTGCAGGCTGGCTGCACGCGGCCGGCGTGGTGGCAtgcggggaggcgagat                                                         >  1:387142/1‑138 (MQ=255)
                                                                                             agGCTGACGACAGTGAAAGTGAACTACAGGAGGCGATCTTCTGCGGATTATGGCGGGTGCGCAGACGTCGCGGCGAAAAGTTGCTGGAGGACAAACTGGAGGCTGGCTGCGCGCCGCTGGCGTTGTGGCAGGCGGCAACGCAAAAtctc                                                      <  2:47217/149‑1 (MQ=255)
                                                                                             agGCTGACGACAGTGAAAGTGAAATACAGGAGGCGATCTTCTGCGGATTATGGCGGGTGCGCAGACGTCGCGGCGAAAAGTTGCTGGAGGACAAACTGGAGGCTGGCTGCGCGCCGCTGGCGTTGTGGCAGGCGGCAACGCAAAAtctc                                                      <  2:10812/149‑1 (MQ=255)
                                                                                             agGCTGACGACAGGGAAAGTGAAATACAGGAGGCGATCTTCTGCGGATTATGGCGGGTGCGCAGACGTCGCGGCGAAAAGTTGCTGGAGGACAAACTGGAGGCTGGCTGCGCGCCGCTGGCGTTGTGGCAGGCGGCAACGCAAAAtctc                                                      <  2:171793/149‑1 (MQ=255)
                                                                                               gCTGACGACAGTGAAAGTGAAATACAGGAGGCGATCTTCTGCGGATTATGGCGGGTGCGCAGACGTCGCGGCGAAAAGTTGCTGGAGGACAAACTGGAGGCTGGCTGCGCGCCGCTGGCGTTGTGGCAGGCGGCAACGCAAAATCTCtt                                                    >  2:576336/1‑149 (MQ=255)
                                                                                               gCTGACGACAGTGAAAGTGAAATACAGGAGGCGATCTTCTGCGGATTATGGCGGGTGCGCAGACGTCGCAGCGTAAAGTTGCTGGAGGACAAACTGGAGGCTGGCTGCGCGCCGCTGGCGGTGGGGCCGGCGGCAACGGAAAAtcttct                                                    >  2:735501/1‑146 (MQ=255)
                                                                                                 tGACGACAGTGAAAGTGAAATACAGGAGGCGATCTTCTGCGGATTATGGCGGGTGCGCAGACGTCGCGGCGAAAAGTTGCTGGAGGACAAACTGGAGGCTGGCTGCGCGCCGCTGGCGTTGTGGCAGGCGGCAACGCAAAATCTCTTGc                                                  >  2:153409/1‑149 (MQ=255)
                                                                                                 tGACGACAGTGAAAGTGAAATACAGGAGGCGATCTTCTGCGGATTATGGCGGGTGCGCAGACGTCGCGGCGAAAAGTTGCTGGAGGACAAACTGGAGGCTGGCTGCGCACCGCTGGCGTTGTGGCAGGCGGCAACGCAAAATCTCTTGc                                                  >  2:255345/1‑149 (MQ=255)
                                                                                                          tgaaagtgaaaTACAGGAGGCGATGTTCTGCGGATTATGGCGGGTGCGCAGACGTCGCGGCGAAAAGTTGCTGGAGGACAAACTGGAGGCTGGCTGCGCGCCGCTGGCGTTGTGGCAGGCGGCAACGCAAAATCTCTTGCCGACAGAtt                                         >  2:644849/1‑149 (MQ=255)
                                                                                                           gacagtgaaaTACAGGAGGCGGTCTTCTGCGGATTATGGCGGGTGCGCAGACGTCGCGGCGAAAAGTTGCTGGAGGACAAACTGGAGGCTGGGTGCGCGGCGCTGGCGTTCTGGCTGGTGGCAACGCAAACTCTCTTGCGGACAGATTc                                        <  1:273399/146‑1 (MQ=255)
                                                                                                              agtgaaaTACAGGAGGGGATCTTCTGCGGATTATGGCGGGTGCGCAGACGTCGCGGCGAAAAGTTGCTGGAGGACAAACTGGAGGCTGGCTGCGCGCCGCTGGCGTTGTGGCAGGCGGCAACGCAAAATCTCTTGCCGACAGATTCGCt                                     >  2:549450/1‑149 (MQ=255)
                                                                                                                  aaatcgcggaggCGGTCTTCTGCGGATTCTGGCGGGTGCGCAGACGTCGCGGCGAAAAGTTGCTGGAGGACAAACTGGAGGCTGGCTGCGCGCCGCTGGCGTTGTGGCAGGCGGCAACGCAAAATCTCTTGCCGACAGATTCGCTGTTa                                 <  1:303170/142‑1 (MQ=255)
                                                                                                                        aggaggCGATCTTCTGCGGATTATGGCGGGTGCGCAGACGTCGCGGCGAAAAGTTGCTGGAGGACAAACTGGAGGCTGGCTGCGCGCCGCTGGCGTTGTGGCAGGCGGCAACGCAAAATCTCTTGCCGACAGATTCGCTGGTAccgccg                           >  1:793975/1‑149 (MQ=255)
                                                                                                                        aggaggCGATCTTCTGCGGATTATGGCGGGTGCGCAGACGTCGCGGCGAAAAGTTGCTGGAGGACAAACTGGAGGCTGGCTGCGCGCCGCTGGCGGTGTGGCAGGCGGCAACGCAAAATCTCTTGCCGACAGATTCGCTGTTGccgccg                           >  1:708261/1‑149 (MQ=255)
                                                                                                                            gggcaTCTTCTGCTGATTATGGCTGGTGCGCAGACGTCGTGGCGAAAAGGTGCTGGAGGACAAACTGGAGGCTGGCTGCGCGCCGCTGTCGTTGTGGCAGGCGGCAACGCAAAATCTCTTGCCGACAGATTCGCTGTTACCGCCGCCCa                       <  2:609918/145‑1 (MQ=255)
                                                                                                                              cGATCTTCTGCGGATTATGGTGGGTGCGCAGACGTCGCGGCGAAAAGTTGCTGGAGGACAAACTGGAGGCTGGCTGCGCGCCGCTGGCGTTGTGGCAGGCGGCAACGCAAAATCTCTTGCCGACAGATTCGCTGTTACCGCCGCCCAtt                     <  2:48644/149‑1 (MQ=255)
                                                                                                                              cGATCTTCTGCGGATTATGGTGGGTGCGCAGACGTCGCGGCGAAAAGTTGCTGGAGGACAAACTGGAGGCTGGCTGCGCGCCGCTGGCGTTGTGGCAGGCGGCAACGCAAAATCTCTTGCCGACAGATTCGCTGTTACCGCCGCCCAtt                     <  2:48560/149‑1 (MQ=255)
                                                                                                                                    tctGCGGATTATGGCGGGTGCGCAGACGTCGCGGCGGAAAGTTGCTGGAGGACAAACTGGAGGCTGGCTGCGCGCCGCTGGCGGTGGGGCAGGCGGCAACGCAAAATCTCTTGCCGACAGATTCGCTGGTACCGCCTCCCATTGATGGc               >  1:628129/1‑149 (MQ=255)
                                                                                                                                    tctGCGGATTATGGCGGGTGCGCAGACGTCGCGGCGAAAAGTTGCCGGAGGACAAACCGGAGGCTGGCGGCGCGCCGCTGGCGTTGTGGCAGGCGGCAACGCAAAATCTCTTGCCGACAGATTCCCCGGTACCGCCGCCCCTTGATgcc               >  1:71932/1‑147 (MQ=255)
                                                                                                                                       gCGGATTATGGCGGGTGCGCAGACGTCGCGGCGAAAAGTTGCTGGAGGACAAACTGGAGGCTGGCTGCGCGCCGCTGGCGTTGTGGCAGGCGGCAACGCCAAATCTCTTGCCGACCGATTCGCTGTTACCGCCGCCCATTGATGGCCtg            >  2:820765/1‑149 (MQ=255)
                                                                                                                                             tATGGCGGGTGCGCAGCCGTCGGGGCGAAAAGTTGCTGGAGGACAAACTGGAGGCTGGCTGCGCGCCGCTGGCGTTGTGGCAGGCGGCAACGCAAAATCTCTTGCCGACAGATTCGCTGTTACCGCCGCCCATTGATGGCCTGATGAAt      <  2:95237/149‑1 (MQ=255)
                                                                                                                                                 gCGGGCGCGCAGACGTCGCGGCGAAAAGTTGCTGGAGGACAAACTGGAGGCTGGCTGCGCGCCGCTGGCGTTGTGGCAGGCGGCAACGCAAAATCTCTTGCCGACAGATTCGCTGTTACCGCCGCCCATTGATGGCCTGATGAATGGcc  >  1:374559/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
TGCTGGCAGTCGATCTGTTAAATCTCAACGCCGACGATCGTCACTTTATCAATACGCTTCTCGGGGAAGGGGAAGTGTCAGTGCGCATTCAGCAGGCTGACGACAGTGAAAGTGAAATACAGGAGGCGATCTTCTGCGGATTATGGCGGGTGCGCAGACGTCGCGGCGAAAAGTTGCTGGAGGACAAACTGGAGGCTGGCTGCGCGCCGCTGGCGTTGTGGCAGGCGGCAACGCAAAATCTCTTGCCGACAGATTCGCTGTTACCGCCGCCCATTGATGGCCTGATGAATGGCC  >  CP009273/1032413‑1032706

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑