Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP009273	3,019,631	G→T	M169I (ATG→ATT)	guaD →	guanine deaminase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	3,019,631	0	G	T	100.0%	167.0 / NA	54	M169I (ATG→ATT)	guaD	guanine deaminase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (27/27);  total (27/27)

AAATGTCGGCGTTCTTCATCAAGCAGCTTTTACGTAACGGAACCACCACGGCGCTGGTGTTTGGCACTGTTCATCCGCAATCTGTTGATGCGCTGTTTGAAGCCGCCAGTCATATCAATATGCGTATGATTGCCGGTAAGGTGATGATGGACCGCAACGCACCGGATTATCTGCTCGACACTGCCGA‑AAGCAGCTATCACCAAAGCAAAGAACTGATCGAACGCTGGCACAAAAATGGTCGTCTGCTATATGCGATTACGCCACGCTTCGCCCCGACCTCATCTCCTGAAC  >  CP009273/3019486‑3019776                                                                                                                                                  |                                                                                                                                                   aaaTCTCGGCGTTCCTCAACAAGCAGCTTTTACGTAACGGAACCACCACGGCGCCGGTGTTTGGCACTGTTCATCCGCAATCTGTTGATGCGCTGTTTGAAGCCGCCAGTCATATCAATATGCATATGATTGCCGGTAAGGTGattatg                                                                                                                                                 <  1:459455/149‑1 (MQ=255)      tCGGCGTTCTTCATCAAGCAGCTTTTACGTAACGGAACCACCACGGCGCTGGTGTTTGGCACTGTTCATCCGCAATCTGTTGATGCGCTGTTTGAAGCCGCCAGTCATATCAATATGCGTATGATTGCCGGAAAGGTgattat                                                                                                                                                  >  2:259281/1‑143 (MQ=255)        ggCGTTCTTCATCAAGCAGCTTTTACGTAACGGAACCACCACGGCGCTGGTGTTTGGCACTGTTCATCCGCAATCTGTTGATGCGCTGTTTGAAGCCGCCAGTCATATCAATATGCGTATGATTGCCGGTAAGGAGATTATGGACCGCa                                                                                                                                          >  1:887310/1‑149 (MQ=255)        ggCGTTCTTCATCAAGCAGCTTTTACGTAACGGAACCACCACGGCGCTGGTGTTCAGCACTGTTCATCCGCAATCTGTTGATGCGCTGTTTGAAGCCGCCAGTCATATCAATATGCGTATGATTGCCGGTAAGGTGATTATGGACCGCa                                                                                                                                          >  1:180053/1‑149 (MQ=255)              cttcATCAAGCAGCTTTTACGTAACGGAACCACCCCGGCGCTGGTGTTTGGCACTGTTCATCCGCAATCTGTTGATGCGCCGTTTGAGGCCGCCATTCATATCAATATGCTGATGATTTGCGGTAAGGGGATTATGGACGGCa                                                                                                                                          >  2:287771/1‑143 (MQ=255)                     aaGCAGCTTTTACGTAACGGAACCACCACGGCGCTGGTGTTTGGCACTGTTCATCCGCAATCTGTTGATGCGCGGTTTGAAGCCGCCAGTCATATCAATATGCGTATGATTGCCGGTAAGGTGATTATGGACCGCAACGCAcc                                                                                                                                   >  2:262554/1‑143 (MQ=255)                        cagcTTTTACGTCACGGAACCACCACGGCGCTGGTGTTTTGCACTGTTCTTACGCAATCTGTTGATGCGCTGGTTGAAGCCGCCAGTCATATCAATATGCGTATTATTGCCGGTAAGGGGATTATGGACCGCAACGAACCGGa                                                                                                                                >  2:282084/1‑143 (MQ=255)                        cagcTTTTACGTAACGGAACCACCACGGCGCTGGTGTTTGGCACTGTTCATCCGCAATCTGTTGATGCGCTGTTTGAAGCCGCCAGTCGTATCAATATGCGTATGATTGCCGGTAAGGTGATTATGGACCGCAACGCACCGGATTATCt                                                                                                                          <  1:819200/149‑1 (MQ=255)                           cTTTTACGTAACGGAACCACCACGGCGCTGGTGTTTGGCACTGTTCATCCGCAATCTGTTGATGCGCTGTTTGAAGCCGCCAGTCATATCAATATGCGTATGATTGCCGGTAAGGTGATTATGGACCGCAACGCACCGGAttt                                                                                                                             >  2:50433/1‑142 (MQ=255)                           cTTTTACGTAACGGAACCACCACGGCGCTGGTGTTTGGCACTGTTCATCCGCAATCTGTTGATGCGCTGTTTGAAGCCGCCAGTCATATCAATATGCGTATGATTGCCGGTAAGGTGATTATGGACCGCAACGCACCGGATTATCTGCt                                                                                                                       >  1:417236/1‑149 (MQ=255)                                aagTAACGGACCCTCCACGGCGCTGGTGTTTGGCACTGTTCATCCGCAATCTGTTGATGCGTTGTTTGAAGCCGCCAGTCATATCAATATGCGTATGATTGCAGCGAAGGTGATTATGGAGCTCAACGCACCGGATTATCTGc                                                                                                                        <  2:345167/141‑1 (MQ=255)                                 cGTAACGGAACCACCACGGCGCTGGTGTTTGGCACTGTTCATCCGCAATCTGTTGATGCGCTGTTTGAAGCCGCCAGTCATATCAATATGCGTATGATTGCCGGTAAGGTGATTATGGACCGCAACGCACCGGATTATCTGCt                                                                                                                       <  2:4907/143‑1 (MQ=255)                                   tAACGGAACCACCACGGCGCTGGTGTTTGGCACTGTTCATCCGCAATCTGTTGATGCGCTGTTTGAAGCCGCCAGTCATATCAATATGCGTATGATTGCCGGTAAGGTGATTATTGACCGCAACGCACCGGATTATCTGCTCg                                                                                                                     >  2:528027/1‑143 (MQ=255)                                   tAACGGAACCACCACGGCGCTGGTGTTTGGCACTGTTCATCCGCAATCTGTTGATGCGCTGTTTGAAGCCGCCAGTCATATCAATATGCGTACGCTTGCCGGTACGGTGATTATGGACCGCAACGCACCGGATCATCTGCTCg                                                                                                                     >  2:250505/1‑143 (MQ=255)                                    aaCGGAACCACCACGGCGCTGTTGTTTGGCAATGTTCATCCGCAATCTGTTGAGGCGTTGTTTGAAGCCGCCAGTTATATCAATATGCGTATGATTGCCGGTAAGGTGATTATGGACCGCAACGCACCGGATTATCTGCTCGa                                                                                                                    <  2:360241/143‑1 (MQ=255)                                    aaCGGAACCACCACGGCGCTGGTGTTTGGCACTGTTCATCCGCAATCTGTTGATGCGCTGTTTGAAGCCGCCAGTCATATCAATATGCGTATGATTGCCGGTAAGGTGATTATGGACCGCAACGCACCGGATTATCTGCTCGa                                                                                                                    <  2:182915/143‑1 (MQ=255)                                     aCGGAACCACCACGGCGCTGGTGTTTGGCACTGTTCATCCGCAATCTGTTGATGCGCTGTTTGAAGCCGCCAGTCATATCAATATGCGTATGATTGCCGGTAAGGTGATTATGGACCGCAACGCACCGGATTATCTGCTCGACACTGcc                                                                                                             <  1:370933/149‑1 (MQ=255)                                           ccaccaCGGCGCTGGTGTTTGGCACTGTTCATCCGCAATCTGTTGATGCGCTGTTTGAAGCCGCCAGTCATCTCAATATGCGTATGATTGCCGGTAAGGTGATTATGGACCGCAACGCACCGGATTATCTGCCCGACACTGcc                                                                                                             <  2:733861/143‑1 (MQ=255)                                               cgcgcctcTGGTGTTTGTCACGGTTCATCCGCAATCTGTTGATGCGCTGTTTGAAGCCGCCAGTCATATCAACATGCGTCTGATTGCCGGTAAGGGGATTATGGACCGCAACGCACCGGATTATCTGCTCGACACTGCCGa‑aa                                                                                                        <  2:428920/136‑1 (MQ=255)                                               cacGGCGCTGGTGTTTGGCACTGTTCATCCGCAATCTGTTGATGCGCTGTTTGAAGCCGCCAGTCATATCAATATGCGTATGATTGCCGGTAAGGTGATTATGGACCGCCACGCACCGGATGAGCTGCTCGACACTGCCGa‑aa                                                                                                        <  2:699770/143‑1 (MQ=255)                                                     gcTGGTGTTTGGCACGTTTCATCCGCAATCTGTTGATGCGCTGTTTGAAGCCGCCAGTCATATCAATATGCGTATGATTGCCGGTAAGGTGATTATGGACCGCAACGCACCGGATTATCTGCTCGACACTGCCGA‑AAGCAGCt                                                                                                  <  2:425947/143‑1 (MQ=255)                                                      cTGGTGTTTGGCACTGTTCATCCGCAATCTGTTGATGCGCTGTTTGAAGCCGCCAGTCATATCAATATGCGTATGATTGCCGGTAAGGTGATTATGGACCGCAACGCACCGGATTATCTGCTCGACACTGCCGA‑AAGCAGCTa                                                                                                 <  2:612000/143‑1 (MQ=255)                                                       tGGTGTTTGGCACTGTTCATCCGCAATCTGTTGATGCGCTGTTTGAAGCCGCCAGTCATATCAATATGCGTATGATTGCCGGTAAGGTGATTATGGACCGCAACGCACCGGATTATCTGCTCGCCACTGCCGA‑AAGCAGCTAt                                                                                                >  2:252138/1‑143 (MQ=255)                                                        ggTGTTTGGCACGGTTCATCCCCCACCTGTTGATGCTGTGTTCGAAGCCCCCAGTCATATCAAGATGCGTATGATTGCCGGTAAGGTGATTATGGACCGCAACGCACCGGATTATATGCTCGACACTGCCGA‑AAGCAGCTATCACcaaa                                                                                         <  1:816850/149‑1 (MQ=255)                                                            tttGGCACTGTTCATCCGCAATCTGTTGATGCGCTGTTTGAAGCCGCCAGTCATATCAATATGCGTATGATTGCCGGTAAGGTGATTATGGACCGCAACGCACCGGATTATCTGCTCGACACTGCCGA‑AAGCAGCTATCACcaaagcaa                                                                                     >  1:307176/1‑149 (MQ=255)                                                            tttGGCACTGTTCATCCGCAATCTGTTGATGCGCTGTTTGAAGCCGCCAGTCATATCAATATGCGTATGATTGCCGGTAAGGTGATTATGGACCGCAACGCACCGGATTATCTGCTCGACACTGCCGA‑AAGCAGCTATCACca                                                                                           >  2:385724/1‑143 (MQ=255)                                                             ttGGCACTGTTCATCCGCAATCTGTTGATGCGCTGTTTGAAGCCGCCAGTCATATCAATATGCGTATGATTGCCGGTAAGGTGATTATGGACCGCAACGCACCGGATTATCTGCTCGACACTGCCGA‑AAGCAGCTATCACcaa                                                                                          <  2:363149/143‑1 (MQ=255)                                                              tGGAACTGTTTTTCCGCAATCTGTTGATGCGCTGTTTGAAGCCGCCAGTCATATCAATATGCGTATGATTGCCGGTAAGGTGATTATGGACCGCAACGCACCGGTTTATCTGCTCGACACTGCCGA‑AAGCAGCTATCACcaaa                                                                                         <  2:333497/143‑1 (MQ=255)                                                                   cTGTTCATCCGCAATCTGTTGATGCGCTGTTTGAAGCCGCCAGTCATATCAATATGCGTATGATTGCCGGTAAGGTGATTATGGACCGACACGCACAGGAGTATCTGCTCGACACTGCCGA‑AAGCAGCTATCACCAAAGCAAAGAACTg                                                                              <  1:715589/149‑1 (MQ=255)                                                                      ttCATCCGCAATCTGTTGATGCGCTGTTTGAAGCCGCCAGTCATATCAATATGCGTATGATTGCCGGTAAGGTGATTATGGACCGCAACGCACCGGATTATCTGCTCGACACTGCCGA‑AAGCAGCTATCACCAAAGCAAAGaa                                                                                 <  2:154418/143‑1 (MQ=255)                                                                       tCATCCGCAATCTGTTGATGCGCTGTTTGAAGCCGCCAGTCATATCAATATGCGTATGATTGCCGGTAAGGTGATTATGGACCGCAACGCACCGGATTATCTGCTCGACACTGCCGA‑AAGCAGCTATCACCAAAGCAAAGaaa                                                                                <  2:112018/143‑2 (MQ=255)                                                                                 tCTGTTGATGCGCTGTTTGAAGCCGCCAGTCATATCAATATGCGTATGATTGCCGGTAAGGTGATTATGGACCGCAACGCACCGGATTATCTGCTCGACACTGCCGA‑AAGCAGCTATCACCAAAGCAAAGAACTGATCGAACGCTGGca                                                                >  1:570775/1‑149 (MQ=255)                                                                                 tCTGTTGATGCGCTGTTTGAAGCCGCCAGTCATATCAATATGCGTATGATTGCCGGTAAGGTGATTATGGACCGCAACGCACCGGAGTATCTGCTCGACACTGCCGA‑AAGCAGCTATCACCAAAGCAAAGCACTTATCGAACGCTGGca                                                                >  1:2867/1‑149 (MQ=255)                                                                                    gTTGATGCGCTGTTTGAAGACGCCAGTCATATCAATATGCGTATGATTGCCGGTAAGGTGATTATGGACCTCAACGCACCGGATTATCTGCTCGACACTGCCGG‑AAGCAGCTATCCCCAAACCAACGAACTGATCGAACACTg                                                                   >  2:473239/1‑143 (MQ=255)                                                                                     ttGATGCGCTGTTTGAAGCCGCCAGTCATATCAATATGCGTATGATTGCCGGTAAGGTGATTATGGACCGCAACGCACCGGATTATCTGCTCGACACTGCCGA‑AAGCAGCTATCACCAAAGCAAAGAACTGATCGAACGCTGGCACaaa                                                            >  1:182123/1‑149 (MQ=255)                                                                                       tattcgcTGAGTGAAGCCTCCAGTCATAGCAATGTGCGTAGGATTGCCGGTAAGGTGATTATGGACCGCAACGCACCGGATTATCTGCTCGACACTGCCGACAAGCA‑CTAACACTTAAGCAAAGAACTGATCGAACGCTGGca                                                                >  2:584550/5‑143 (MQ=255)                                                                                       gATGCGCTGTTTGAAGCCGCCAGTCATATCAATATGCGTATGATTGCCGGTAAGGTGATTATGGACCGCAACGCACCGGATTATCTGCTCGACACTGCCGA‑AAGCAGCTATCACCAAAGACAAGAACTGATCGAACGCTGGCACaaaaa                                                          >  1:806550/1‑149 (MQ=255)                                                                                         tGCGCTGTTTGAAGCCGCCAGTCATATCAATATGCGTATGATTGCCGGTAAGGTGATTATGGACCGCAACGCACCGGATTATCTGCTCGACACTGCCGA‑AAGCAGCTATCACCAAAGCAAAGATCTGATCGAACGCTGGCACAAAAATg                                                        <  1:459801/149‑1 (MQ=255)                                                                                                    aaGCCGCCAGTCATATCAATATGCGTATGATTGCCGGTAAGGTGATTATGGACCGCAACACACCTGATTATCTGCTCGACACTGCCGA‑AAGCAGCTATCACCAAAGCAAAGAACTGATCGAACGCTGGCACCAAAATGGTCGGCTGCta                                             >  1:179181/1‑149 (MQ=255)                                                                                                     aGCCGCCAGTCATATCAATATGCGTATGATTGCCGGTAAGGTGATTATGGACCGCAACGCACCGGATTATCTGCTCGACACTGCCGA‑AAGCAGCTATCACCAAAGCAAAGAACTGATCGAACGCTGGCACAAAAATGgtcgtc                                                  >  2:427853/1‑143 (MQ=255)                                                                                                           cAGTCATATCAATATGCGTATGATTGCCGGTAAGGTGATTATGGACCGCAACGCACCGGATTATCTGCTCGACACTGCCGA‑AAGCAGCTATCACCAAAGCAAAGAACTGATCGAACGCTGGCACAAAAATGGTCGTCTGCTATATGCGa                                      <  1:227405/149‑1 (MQ=255)                                                                                                                 tatCAATATGCGTATGATTGCCGGTAAGGTGATTATGGACCGCAACGCACCGGATTATCTGCTCGACACTGCCGA‑AAGCAGCTATCACCAAAGCAAAGAACTGATCGAACGCTGGCACAAAAATGGTCGTCTGCTATATGCGa                                      >  2:105177/1‑143 (MQ=255)                                                                                                                 tatCAATATGCGTATGATTGCCGGTAAGGTGATTATGGACCGCAACGCACCGGATTATCTGCTCGACACTGCCGA‑AAGCAGCTATCACCAAAGCAAAGAACTGATCGAACGCTGGCACAAAAATGGTCGTCTGCTATATGCGATTACGc                                >  1:538427/1‑149 (MQ=255)                                                                                                                  atCAATATGCGTATGATTGCCGGTAAGGTGATTATGGACCGCAACGCACCGGATTATCTGCTCGACACTGCCGA‑AAGCAGCTATCACCAAAGCAAAGAACTGATCGAACGCTGGCACAAAAATGGTCGTCTGCTATATGCGATTACGca                               >  1:93648/1‑148 (MQ=255)                                                                                                                  atCAATAGGCTTATGATTGCCCGTAAGGTGATTATGGACCGCAACGCACCGGATTATCTGCTCGACACTGCCGA‑AAGCAGCTATCACCAAAGCAAAGAACTGATCGAACGCTGGCACAAAAATGGTCGTCTGCTATATGCGATTACGcc                               <  1:537905/149‑1 (MQ=255)                                                                                                                   tCAATATGCGTATGATTGCCGGTAAGGTGATTATGGACCGCAACGCACCGGATTATCTGCTCGACACTGCCGA‑AAGCAGCTATCACCAAAGCAAAGAACTGATCGAACGCTGGCACAAAAATGGTCGTCTACTATATGCGATTACGCCa                              <  1:71915/149‑1 (MQ=255)                                                                                                                     aaTATGCGTATGATTGCCGGTAAGGTGATTATGGACCGCAACGCACCGGATTATCTGCTCGACACTGCCGA‑AAGCAGCTATCACCAAAGCAAAGAACTGATCGAACCCTGGCCCAAAAATGGTCGTCTGCTATATGCGATTACGCCACg                            >  1:16661/1‑149 (MQ=255)                                                                                                                      atatGCGTATGATTGCCGGTAAGGTGATTATGGACCGCAACGCACCGGATTATCTGCTCGACACTGCCGA‑AAGCAGCTATCACCAAAGCAAAGAACTGATCGAACGCTGGCACAAAAATGGTCGTCTGCTATATGCGATTACGCCACGc                           >  1:664628/1‑149 (MQ=255)                                                                                                                            gTATGATTGCCGGTAAGGTGATTATGGACCGCAACGCACCGGATTATCTGCTCGACACTGCCGA‑AAGCAGCTATCACCAAAGCAAAGAACTGATCGAACGCTGGCACAAAAATGGTCGTCTGCTATATGCGATTAAGCCACGCTTCGcc                     >  1:157658/1‑149 (MQ=255)                                                                                                                                gATTGCCGGTAAGGTGATTATGGACCGCAACGCACCGGATTATCTGCTCGACACTGCCGA‑AAGCAGCTATCACCAAAGCAACGAACTGATCGAACGCTGGCACAAACATGGTCGTCTGCTATATGCGATTACGCCACGCTTCGCCCCGa                 <  1:722602/149‑1 (MQ=255)                                                                                                                                gATTGCCGGTAAGGTGATTATGGACCGCAACGCACCGGATTATCTGCTCGACACTGCCGA‑AAGCAGCTATCACCAAAGCAAAGAACTGATCGAACGCTGGCACAAAAATGGTCGTCTGCTATATGCGATTACGCCACGCTTCGCCCCGa                 <  1:312188/149‑1 (MQ=255)                                                                                                                                    gctgggacggcgatgatGGCCCCCACGGCACCGGATTATCGGCTCGACACTGCCGA‑AAGCAGCTACCACCAAAGCAAAGACCTGATCGAACGCTGGCACAAAAATGGTCGTCTGCTATATGCGATTACGCCACGCTTCGCCCCGACCTc             <  1:57876/138‑1 (MQ=255)                                                                                                                                    gCCGGTAAGGTGATTATGGACCCCAACGCACCGGATTATCTGCTCGACACTGCCGA‑AAGCAGCTATCACCAAAGCAAAGAACTGATCGAACGCTGGCACAAAAATGGTCGTCTGCTATATGCGATTACGCCACGCTTCGCCCCGACCTc             <  1:56326/149‑1 (MQ=255)                                                                                                                                      cGGTAAGGTGATTATGGACCGCAACGCACCGGATTATCTGCTCGACACTGCCGA‑AAGCAGCTATCACCAAAGCAAAGAACTGATCGAACGCTGGCACAAAAATGGTCGTCTGCTATATGCGATTACGCCACGCTTCGCCCCGACCTCAt           >  1:497437/1‑149 (MQ=255)                                                                                                                                       ggTAAGGTGATTATGGACCGCAACGCACCGGATTATCTGCTCGACACTGCCGA‑AAGCAGCTATCACCAAAGCAAAGAACTGATCGAACGCTGGCACAAAAATGGTCGTCTGCTATATGAGATTACGCCACGCTTCGCCCCGACCTCAtc          <  1:478162/149‑1 (MQ=255)                                                                                                                                         tAAGGTGATTATGGACCGCAACGCACCGGATGATCTGCTCGACACTGCCGA‑AAGCAGCTATCACCAAAGCAAAGAACGGATCGAACGCTGGCACAAAAATGGTCGTCTGCTATATGCGATTACGCCACGCTTCGCCCCGACCTCAtctc        <  1:129165/149‑1 (MQ=255)                                                                                                                                         tAAGGGGATTATGGACCGCACCGCACCGGATTATCTGCTCGACACTGCCGA‑AAGCAGCTATCACCAAAGCAAAGAACTGATCGAACGCTGGCACAAAAATGGTCGTCTGCTATATGCGATTACGCCACGCTTCGCCCCGACCTCAtctc        <  1:116105/149‑1 (MQ=255)                                                                                                                                              tgattaTGGACCGCACCGCACCGGATTATCTGCTCGACACTGCCGA‑AAGCAGCTATCACCAAAGCAAAGAACTGATCGAACGCTGGCACAAAAATGGTCGTCTGCTATATGCGATTACGCCACGCTTCGCCCCGACCTCATCTCCTGaa   <  1:618932/149‑1 (MQ=255)                                                                                                                                               gattatGGACCGCAACGCACCGGATTATCTGCTCGACACTGCCGA‑AAGCAGCTATCACCAAAGCAAAGAACTGATCGAACGCTGGCACAAAAATGGTCGTCTGCTATATGCGATTACGCCACGCTTCGCCCCGACCTCATCTCCTGAAc  <  1:432000/149‑1 (MQ=255)                                                                                                                                                  |                                                                                                                                                   AAATGTCGGCGTTCTTCATCAAGCAGCTTTTACGTAACGGAACCACCACGGCGCTGGTGTTTGGCACTGTTCATCCGCAATCTGTTGATGCGCTGTTTGAAGCCGCCAGTCATATCAATATGCGTATGATTGCCGGTAAGGTGATGATGGACCGCAACGCACCGGATTATCTGCTCGACACTGCCGA‑AAGCAGCTATCACCAAAGCAAAGAACTGATCGAACGCTGGCACAAAAATGGTCGTCTGCTATATGCGATTACGCCACGCTTCGCCCCGACCTCATCTCCTGAAC  >  CP009273/3019486‑3019776

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑