Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009273 2,719,426 T→C intergenic (‑321/+2) kgtP ← / ← rrfG alpha‑ketoglutarate transporter/5S ribosomal RNA of rrnG operon

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,719,4260TC98.5% 196.9 / ‑4.7 66intergenic (‑321/+2)kgtP/rrfGalpha‑ketoglutarate transporter/5S ribosomal RNA of rrnG operon
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base C (37/28);  total (38/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.60e-01

GAAGTGGGCAAGTTGGGGAAGCCGTATCCGTTGCTGAATCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACGGCCGCCAGGCAAATTCTGTTTTAT  >  CP009273/2719287‑2719560
                                                                                                                                           |                                                                                                                                      
gAAGTGGGCAAGTTGGGGAAGCCGTATCCGTTGCTGAATCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGAt                                                                                                                                     >  2:4473/1‑143 (MQ=255)
 aaGTGGGCAAGTTGGGGAGGCCGTATCCGTTGCTGAATCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATg                                                                                                                                    >  2:408819/1‑143 (MQ=255)
  aGTGGGCAAGTCGGGGAAGCCGTATCCGTGGCTGAATCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGAAGATTCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCa                                                                                                                             <  1:488023/149‑1 (MQ=25)
     gggCAAGTTGGGGAAGCCGTATCCGTTGCTGAATCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTGGCATAATTCGATGCCTg                                                                                                                                >  2:532706/1‑143 (MQ=255)
        cAAGTTGGGGAAGCCGTATCCGTTGCTGAATCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCa                                                                                                                             >  2:403143/1‑143 (MQ=255)
          aGTTGGTGAAGCCGTATCCGTTGCTGAAGCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTa                                                                                                                     <  1:14717/149‑1 (MQ=255)
               gggAAGCCGTATCCGTTGCTGAATCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGCCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCt                                                                                                                      >  2:403785/1‑143 (MQ=255)
                ggAAGCCGTATCCGTTGCTGGATCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTCCTCTCg                                                                                                               >  1:99778/1‑149 (MQ=255)
                   aGCCGTTGCCGTCGCTGAATCTCGCAGATGTGGGAGTTTAAGACGCGCATCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCCAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTActc                                                                                                                  <  2:224814/143‑1 (MQ=21)
                    gCCGTATCCGTTGCTGAATCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTActct                                                                                                                 >  2:153007/1‑143 (MQ=255)
                     ccGTATCCGTTGCTGAATCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATgg                                                                                                          >  1:489497/1‑149 (MQ=255)
                     ccGTATCCGTTGCTGAATCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTATGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACtctc                                                                                                                >  2:500874/1‑143 (MQ=255)
                      cGTATCCGTTGCTGAATCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATggg                                                                                                         <  1:381761/149‑1 (MQ=255)
                       gTATCCGTTGCTGAATCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATgggg                                                                                                        <  1:446666/149‑1 (MQ=255)
                       gTATCCGTTGCTGAATCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATgggg                                                                                                        <  1:726690/149‑1 (MQ=255)
                             gTTGCTGAATCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGCTGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGAccc                                                                                                  >  1:265595/1‑149 (MQ=255)
                                 cTGAATCTGGCATATGTGGGAGTATAAGCAAAGCCACTAGTGATCAGGCATTTTTTTCTGCGCCAATGCAACAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGAc                                                                                                    >  2:808172/1‑143 (MQ=18)
                                 cTGAATCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGTTCCTATCCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATGCGATGCCAGGCAGTTCCCTACCCTCGCCTggggcacc                                                                                                    >  2:40170/1‑139 (MQ=21)
                                 cTGAATCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGGCCCcaca                                                                                              >  1:866192/1‑149 (MQ=255)
                                      tCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCCTGGGGAGAcccccc                                                                                               >  2:101278/1‑141 (MQ=255)
                                        tGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCacac                                                                                             >  2:185841/1‑143 (MQ=255)
                                         ggCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATc                                                                                      >  1:155783/1‑149 (MQ=255)
                                           cATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATCTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTTCTCGTGCATGGGCAGACCCCACACTACCATCgg                                                                                    <  1:222744/149‑1 (MQ=25)
                                           cATATGTGGGAGTATAAGACGCGCAGCGTCGCAGCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGACCTTTCGCATAATCCGATGCCTGGCAGTTCCCTACTCCCGCATGGGGAGACCCCACACTACCATCgg                                                                                    <  1:559480/149‑1 (MQ=25)
                                            atatGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCACTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGGTCCCTACTCTCGCATGGGGAGACCCCACACTAcc                                                                                         >  2:299555/1‑143 (MQ=255)
                                             tatGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGgcg                                                                                  <  1:168239/149‑1 (MQ=255)
                                              gtgtgGGCGTACAAGACGCGCAGCGTCGCCTCAGTCTTTTTTTTCTTCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTTGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCAt                                                                                       <  2:637628/142‑1 (MQ=25)
                                                   ggggTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACgg                                                                            <  1:368432/146‑1 (MQ=255)
                                                   ggAGTATAAGACGCGGAGAGTCATATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTAGGGATGACCCTCCGCATAATTCGCTGCCTGGCAGTTCCCTACTCTCGCATGGGGAGAGCCCGCACTACCATCGgcg                                                                                  >  2:457675/1‑143 (MQ=21)
                                                    gAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGGGCCAATGCAAAAAGGCCATGCGTCAGGGTGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGc                                                                           >  1:257642/1‑149 (MQ=255)
                                                    gAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTCCTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGc                                                                           >  1:573667/1‑149 (MQ=255)
                                                       tataAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGtt                                                                        >  1:316716/1‑149 (MQ=255)
                                                         taAGACGCGCAGCGTCGCAGCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACgg                                                                            <  2:530504/143‑1 (MQ=255)
                                                          aaGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGc                                                                           >  2:118457/1‑143 (MQ=255)
                                                           aGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCg                                                                          <  2:840248/143‑1 (MQ=255)
                                                             aCGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACtt                                                                  <  1:96895/149‑1 (MQ=255)
                                                              cgcgCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTc                                                                 >  1:84205/1‑149 (MQ=255)
                                                               gcgcAGCGTCGCATCAGGCATTCTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGCAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCt                                                                <  1:485842/149‑1 (MQ=255)
                                                                   aGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGt                                                            >  1:187211/1‑149 (MQ=255)
                                                                     cGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTc                                                          <  1:501388/149‑1 (MQ=255)
                                                                           agcAGTCATTGTTTTCTGCGCCAGTGCAAAAAGGCCATCCGTTAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTTTCTCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTc                                                          <  2:210264/141‑1 (MQ=21)
                                                                            tCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGTCGCTACGGCGTTTCACTTCTGAGTTCg                                                         <  2:311537/143‑1 (MQ=255)
                                                                            tCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATgg                                                   >  1:256111/1‑149 (MQ=255)
                                                                                gCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATGATTCGATGCGTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCGTCGGCGCTACGGCGTTTCACTTCTGATTTCGGCATggggcc                                               >  1:413506/1‑147 (MQ=21)
                                                                                gCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTc                                               <  1:652972/149‑1 (MQ=255)
                                                                                  aTTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAg                                             >  1:421344/1‑149 (MQ=255)
                                                                                  aTTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAg                                             >  1:421286/1‑149 (MQ=255)
                                                                                   tttttttCCGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATggg                                                  <  2:517313/143‑1 (MQ=255)
                                                                                    ttttttCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGt                                           >  1:355365/1‑149 (MQ=255)
                                                                                    tgtgttCTGCGCCAAAGCAAAAAGGGCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTCCTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGt                                           <  1:249397/145‑1 (MQ=18)
                                                                                           tGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTAGCATGGGGAGACCCCACCCCACCATCGGAGATACGCAGTTACACTTCTTATTTCGGCATTGGGTCAGGtt                                          <  2:75825/143‑2 (MQ=18)
                                                                                             cgcCAATGCAAAAAGGCCAGCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCATTTCCCTACTCTGGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTggg                                        <  2:362737/143‑1 (MQ=255)
                                                                                              gcCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGGGTTCGGCATGGGGTCAGGTGGGACCACcg                                 >  1:383878/1‑149 (MQ=255)
                                                                                                cAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACcgcg                               >  1:129406/1‑149 (MQ=255)
                                                                                                  aTGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGaccac                                   <  2:499837/143‑1 (MQ=255)
                                                                                                    gCAAAAAGGCCATCCGACAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGCGAGACCACCGCGCTAc                           >  1:575315/1‑149 (MQ=255)
                                                                                                              cATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCCCTTCTGAGTTCGGCGTGGGGTCAGGTGGGACCACCGCGCGACGgccgcccag                 >  1:434144/1‑146 (MQ=17)
                                                                                                              cATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACATCTGAGTTCGGCATGGGGCCAGGTCGGACCACCGCGCTACGgcc                       >  2:196779/1‑143 (MQ=255)
                                                                                                                 ccGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACGGCCGCCAGGCaa              >  1:181783/1‑149 (MQ=255)
                                                                                                                  cggcAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACGGCCGCCAGGCaaa             <  1:911909/146‑1 (MQ=255)
                                                                                                                  cGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACGGCCGCCAGGCaaa             <  1:73283/149‑1 (MQ=255)
                                                                                                                         aTGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGATACGGCCGCCAGGCaaaa            >  2:140224/1‑142 (MQ=255)
                                                                                                                            gCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTGCGGCGTTTCACTGCTGAGTTCGGCCTGGGGTCAGGTGGGACCACCGCGCTACGGCCGCCAGGCAACTTCt         >  2:715764/1‑143 (MQ=11)
                                                                                                                             ccTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACGGCCGCCAGGCAAATTCTGTTTTAt  <  1:451555/149‑1 (MQ=37)
                                                                                                                              cTTTCGCATAATTCGATGCCTGGTAGTTCCCTACTCTCGCATGGGGAGCCCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACGGCCGCCAGGCAAATTCTGt       >  2:316448/1‑143 (MQ=255)
                                                                                                                              cTTTCGCATAATTCGATGCCTGGCAGTTCCCGTCGCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACGGCCGCCAGGCAAATTCTGt       <  2:712192/143‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                      
GAAGTGGGCAAGTTGGGGAAGCCGTATCCGTTGCTGAATCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACGGCCGCCAGGCAAATTCTGTTTTAT  >  CP009273/2719287‑2719560

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑