Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP009273	2,718,678	C→G	G143A (GGC→GCC)	kgtP ←	alpha‑ketoglutarate transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	2,718,678	0	C	G	100.0%	163.7 / NA	48	G143A (GGC→GCC)	kgtP	alpha‑ketoglutarate transporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (22/26);  total (22/26)

TTCCATGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCCGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCGGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACACATCATACACACCGATAACAGCA  >  CP009273/2718542‑2718819                                                                                                                                         |                                                                                                                                              ttCCATGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCCGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGGCATAtt                                                                                                                                         >  2:21623/1‑143 (MQ=255)    cATGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCCGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGTCAACTTCACTCATATAGGTGGCGCTGGTGGCATATTCTCCGCCaa                                                                                                                                <  1:131654/149‑1 (MQ=255)     aTGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCCGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGGCATATTCTCCGCCAAc                                                                                                                               >  1:413996/1‑149 (MQ=255)               gtAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCCGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGGCATATTCTCCGCCAACAGATAATccc                                                                                                                     >  1:807829/1‑149 (MQ=255)                       aCGACAACCAGTAGGGCTAGCAGTTGTCCGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGGCACATTCTCCGCCAACAGATAATCCCTg                                                                                                                   <  2:416851/143‑1 (MQ=255)                        cGACAACCAGTAGGGCTAGCAGTTGTCCGCCGATCAACGTCACATACTGAAATGATGCGTAGAAACCTTGGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGGCATATTCTCCGCCAACCGATCATCCCTGa                                                                                                                  >  2:510268/1‑143 (MQ=255)                                 gTAGGGCTAGCAGTTGTCCGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGGCATCACTGCCGCCACAAGATAACCCCACAAATAAAcga                                                                                                         >  2:712612/1‑143 (MQ=255)                                    gggCTAGCAGTTGTCCGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGGCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGc                                                                                                <  1:694096/149‑1 (MQ=255)                                      gCTAGCAGTTGTCCGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATCGGTGGCGCTGGTGGCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCaa                                                                                              <  1:392095/149‑1 (MQ=255)                                        tAGCAGTTGTCCGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCCCTCATCTAGGTGGCGCTGGCGGCATATTCTCCCCCAACAGATCATCCCTGAAATCAACGAGCGAGaa                                                                                                  <  2:112019/143‑1 (MQ=255)                                          gCAGTTGTCCGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGGCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGc                                                                                                >  2:13730/1‑143 (MQ=255)                                              ttGTCCGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGGCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCaata                                                                                            >  2:290715/1‑143 (MQ=255)                                                gTCCGCCGATCAACGTCACATACTGAAAGGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGGCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCaataat                                                                                          <  2:145180/143‑1 (MQ=255)                                                 tCCGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGGCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATg                                                                                         <  2:254436/143‑1 (MQ=255)                                                  ccgccgATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCGGGTGGCACATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCGGAGc                                                                                  <  1:632018/149‑1 (MQ=255)                                                    gccgATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGGCATATTCTCCGCCAACAGATCATCCCTGAAAAAAACTAGCGAGAAGCAATAATGCCGGAGccc                                                                                >  1:397270/1‑149 (MQ=255)                                                    gccgATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGGCATATTCTCCGCCAACAGATAATCCCTGAGATAAACGAGCGCGAAGCAATAATGCCg                                                                                      >  2:706023/1‑143 (MQ=255)                                                     ccgATCAACGTCACATACTGAAATGATGCGTAAACACCTTTGCGCCCTTCAACGGCAACCTCACCCATATAGGTGGCGCTGGTGGCATATTCTCCGCCAACAGATAATCCCCGAACAAAACGAGCGAGAAGCAATCATGCCGGAGcccc                                                                               >  1:199428/1‑148 (MQ=255)                                                      cggtCAACGTCACATACTGGAATGACGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCCCTCATATAGGTGGCGCTGGTGGCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCGGa                                                                                    <  2:169312/140‑1 (MQ=255)                                                        aTCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGGCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCGGAGc                                                                                  <  2:315255/143‑1 (MQ=255)                                                         tCAACGTCACATACTGAAATGATGCGTAAAACCCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGGCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGACCGAGAAGCAATAATGTCGGAGcc                                                                                 <  2:565702/143‑1 (MQ=255)                                                                cacaTACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGGCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCGGAGCCCACGTAc                                                                          <  2:553901/143‑1 (MQ=255)                                                                cacaTACTGAAATGATGAGTAAATACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGGCATATTCTCCGCTAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCGGAGCCCCCGTAc                                                                          <  2:520101/143‑1 (MQ=255)                                                                       tGACATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGGCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCGGAGCCCACGTACCTATAGt                                                                   <  2:544821/143‑1 (MQ=255)                                                                         aaaTGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGGCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCGGAGCCCACGTACCTATAGTTTCATAAc                                                           <  1:177296/149‑1 (MQ=255)                                                                          aaTGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTCGCTCAGTCTCCTCCAACAGAGAATCCCGGAAATAAACGAGCGAGAAGCAATAATGCCGGAGCCCACGTACCTATAGTTTCATAAcc                                                          <  1:671253/149‑1 (MQ=255)                                                                               tgCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGGCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAAAAGGCATAATGCGGGAGCCCACGTGCCAAGAGTGTCAGaag                                                           >  2:268974/1‑142 (MQ=255)                                                                                 cGTAAAACCCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGGCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCGGAGCCCACGTACCTATAGTTTCATAACCTGGGagg                                                   <  1:788867/149‑1 (MQ=255)                                                                                 cGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGGCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCGGAGCCCACGTACCTATAGTTTCATAACCTGGGagg                                                   >  1:636958/1‑149 (MQ=255)                                                                                      aaaCCTCTGCGCCCTTCAACGGCAACTTCCCTCATATAGGTGGCGCTGGTGGCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCGGAGCCCACGTACCTATAGTTTCATAACCTGGGag                                                    <  2:338471/143‑1 (MQ=255)                                                                                       aaCCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGGCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGGGAAGCAATAATGCCGGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCg                                             >  1:650865/1‑149 (MQ=255)                                                                                       aaCCTCTGCGCCCTTAAACGGCAACTTCACTCATATAGGTGGCGCTGGTGGCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCGGAGCCCACGTACCTATAGTTTCATAACCTGGGagg                                                   <  2:432245/143‑1 (MQ=255)                                                                                         ccTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGGCATATTCTCCGCCAACAGATAATACCTGAAATAAACGAGCGAGAAGCAATAATGCCGGAGCCCACGTACCTATAGTTTCATAACCTGGGCGGCAGGCGAt                                           >  1:808264/1‑149 (MQ=255)                                                                                           tttGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGGCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCGGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATaa                                         <  1:24316/149‑1 (MQ=255)                                                                                             tGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGGCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCGGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCg                                             >  2:48088/1‑143 (MQ=255)                                                                                                   cTTCAACGGCAACTTCACTCATACAGGTGGCGCTGGTGGCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCGGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGAt                                 <  1:577401/149‑1 (MQ=255)                                                                                                        aagggaactactcccATGTAGGTGGCGCTGGTGGCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCGGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGa                                  >  2:217986/15‑143 (MQ=255)                                                                                                        aCGGCAACTTCCCTCACATAGGTGGCGCTGGTGGCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCGGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGaa                            <  1:497761/149‑1 (MQ=255)                                                                                                        aCGGCAACTTCACTCATATAGGTGGCGCTGGTGGCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCGGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGaa                            >  1:24554/1‑149 (MQ=255)                                                                                                          ggaaaCTTCACTAATATAGGTGGCGCTGGTTGCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAAGGCCGGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATc                                <  2:66961/140‑1 (MQ=255)                                                                                                              aCTTCACTCATATAGGTGGCGCTGGTGGCATATGCACCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCGGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACAcat                      <  1:434458/149‑1 (MQ=255)                                                                                                                     tCATATAGGTGGCGCTGGTGGCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCGGAGCCCACGTACCTATAGTTTCATAACCTGGGATGCAGGCGATAACCAGCGATCCGAAACAcatc                     <  2:370252/143‑1 (MQ=255)                                                                                                                     tCATATAGGTGGCGCTGGTGGCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCGGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACACATCATacac               >  1:102005/1‑149 (MQ=255)                                                                                                                       atatAGGTGGCGCTGGTGGCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCGGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACACATCATacacac             >  1:600142/1‑149 (MQ=255)                                                                                                                             gTGGCGCTGGTGGCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCGGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACACATCATACACACCGATaa       >  1:284664/1‑149 (MQ=255)                                                                                                                              tGGCGCTGGTGGCACATTCCCCGCCAACAAATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCGGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACACATCATACACACCGATAAc      <  1:86392/149‑1 (MQ=255)                                                                                                                                gcgcTGGTGGCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCGGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACACATCATACACACCGATAACAg    >  1:378558/1‑149 (MQ=255)                                                                                                                                  gcTGGTGGCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCGGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACACATCATACACACCGATAACAGCa  >  1:597253/1‑149 (MQ=255)                                                                                                                                         |                                                                                                                                              TTCCATGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCCGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCGGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACACATCATACACACCGATAACAGCA  >  CP009273/2718542‑2718819

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑