Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009273 4,183,494 C→A R4L (CGT→CTT)  thiF ← adenylyltransferase, modifies ThiS C‑terminus

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,183,4940CA98.3% 183.6 / NA 59R4L (CGT→CTT) thiFadenylyltransferase, modifies ThiS C‑terminus
Reads supporting (aligned to +/- strand):  ref base C (0/1);  major base A (25/32);  minor base G (0/1);  total (25/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CAGCGCCCGCCAGGTACAGCGCAGCAGGTGTACCCAGCCCGCCCAGACCGATAATCAGCACCTGGCTGTCGAGCAGTTTTTGCTGCCCGTCCAGAGCGATATCGTCGAGCAGGATTTGGCGGCTATAACGCATAAAGTCACGGTCATTCATCGCCAACTCCTGCAATTTCCAGCAACTGTGCCGTTGCCAAACGCCAGTCTGCGGCTTGAGTAATGGCGCTGACGACGGCGATACTGCCGACACCCGTTGCTATCACCGCAGGCGCGCGTGCCAGACTGAT  >  CP009273/4183354‑4183634
                                                                                                                                            |                                                                                                                                            
cAGCGCCCGCCAGGTACAGCGCAGCAGGTGTACCCAGCCCGCCCAGACCGATAATCAGCACCAGCCTGTCGAAAAGTTTTTGCTGCCCGTCCAGAGCGATATCGTCGAGCAGGATTTGGCGGCTATAACACATCAAGTCAAGGacattc                                                                                                                                      >  1:219035/1‑149 (MQ=255)
  gcgcCCGCCAGGTACAGCGCAGCAGGTGTACCCAGCCCGCCCAGACCGATAATCAGCACCTGGCTGTCGAGCAGTTTTTGCTGCCCGTCCAGAGCGATATCGTCGAGCAGGATTTGGCGGCTATAACGCATAAAGTCAAGGtcattcat                                                                                                                                    <  1:143478/149‑1 (MQ=255)
        gccAGGTACAGCGCAGCAGGTGTACCCAGCCCGCCCAGACCGATAATCAGCACCTGGCTGTCGAGCAGTTTTTGCTGCCCGTCCAGAGCGATATCGTCGAGCAGGATTTGGCGGCTATAACGCATAAAGGCAAGGtcattcat                                                                                                                                    >  2:306467/1‑143 (MQ=255)
           aGGTACAGCGCAGTAGGTGTACCCAGCCCGCCCAGACCGACAATCAGCACCTGGCTGTCGAGCAGTTTTTGCTGCCCGTCCAGAGCGATATCGTCGAGCAGGATTTGGCGGCTATAACGCATAAAGTCAAGGTCATTCATCGCCAACTc                                                                                                                           <  1:759564/149‑1 (MQ=255)
             gTACCGCGCAGCAGGTGTACCCAGCCCGCCCAGACCGATAATCAGCACCTGGCTGTCGAGCAGTTTTTGCTGCCCGTCCAGAGCGATATCGTCGAGCAGGATTTGGCGGCTATACCGCATAAAGTCAAGGTCATTCATCGCCAACTCCt                                                                                                                         <  1:722183/149‑1 (MQ=255)
             gTACAGCGCAGCAGGTGTACCCAGCCCGCCCAGACCGATAATCAGCACCTGGCTGTCGAGCAGTTTTTGCTGCCCGTCCAGAGCGATATCGTCGAGCAGGATTTGGCGGCTATAACGCATAAAGTCAAGGTCATTCATTGCCAACTCCt                                                                                                                         >  1:332106/1‑149 (MQ=255)
                   cgcAGCAGGTGTACCCAGCCCGCCCAGACCGATAATCAGCACCTGGCTGTCGAGCAGTTTTTGCTGCCCGTCCAGAGCGATATCGTCGAGCAGGATTTGGCGGCTATAACGCATAAAGTCAAGGTCATTCATCGCCAACTCCTGCaact                                                                                                                   >  1:795790/1‑147 (MQ=255)
                     cagcagGTGTACCCAGCCCGCCCAGACCGATAATCAGCACCTGGCTGTCGAGCAGTTTTTGCTGCCCGTCCAGAGCGATATCGTCGAGCAGGATTTGGCGGCTATAACGCATAAAGTCAAGGTCATTCATCGCCAACTCCTGCAATTTc                                                                                                                 <  1:877631/149‑1 (MQ=255)
                      agcagGTGTACCCAGCCCGCCCAGACCGATAATCAGCACCTGGCTGTCGAGCAGTTTTTGCTGCCCGTCCAGAGCGATATCGTCGAGCAGGATTTGGCGGCTATAACGCATAAAGTCAAGGTCATTCATCGCCCACTCGTGCa                                                                                                                      >  2:488471/1‑143 (MQ=255)
                        cagGTGTACCCAGCCCGCCCAGACCGATAATCAGCACCTGGCTGTCGAGCAGTTTTTGCTGCCCGTCCAGAGCGATATCGTCGAGCAGGATTTGGCGGCTATAACGCATAAAGTCAAGGTCATTCATCGCCAACTCCTGCAATTTCCAg                                                                                                              <  1:724184/149‑1 (MQ=255)
                            tgtACCCAGCCCGCCCCGACCGATGATCAGCACCTGGCGGTCGAGCAGTTTTTGCTGCCCGTCCAGAGCGATATCGTCGAGCAGGATTTGGCGGCTATAACGCATAAAGTCAAGGTCATTCATCGCCAACTCCTGCAATTTCCAGCAAc                                                                                                          <  1:776685/149‑1 (MQ=255)
                            tgtACCCAGCCCGCCCAGACCGATAATCAGCACCTGGCTGTCGAGCAGTTTTTGCTGCCCGTCCAGAGCGATATCGTCGAGCAGGATTTGGCGGCTATAACGCATAAAGTCAAGGTCATTCATCGCCAACTCCTGCAATTTcc                                                                                                                <  2:137680/143‑1 (MQ=255)
                              accccctcccgcccAGACCGATAATCAGCCCCGGGCTGTCGAGCAGTTTTTGCTGCCCGTCCAGAGCGATATCGTCGAGCAGGATTTGGCGGCTATAACGCATAAAGTCAAGGTCATTCATCGCCAACTCCTGCAATTTCCAg                                                                                                              <  2:398505/136‑1 (MQ=255)
                                  cAGCCCGCCCAGACCGATAATCAGCACCTGGCTGTCGAGCAGTTTTTGCTGCCCGTCCAGAGCGATATCGTCGAGCAGGATTTGGCGGCTATAACGCATAAAGTCAAGGTCATTCATCGCCAACTCCTGCAATTTCCAGCAACTGTGcc                                                                                                    >  1:755106/1‑149 (MQ=255)
                                  cAGCCCGCCCAGACCGATAATCAGCACCTGGCTGTCGAGCAGTTTTTGCTGCCCGTCCAGAGCGATATCGTCGAGCAGGATTTGGCGGCTATAACGCATAAAGTCAAGGTCATTCATCGCCAACTCCTGCAATTTCCAGCAACTGTGcc                                                                                                    >  1:806636/1‑149 (MQ=255)
                                   aGCCCGCCCAGACCGATAATCAGCACCTGGCTGTCGAGCAGTTTTTGCTGCCCGTCCAGAGCGATATCGTCGAGCAGGATGTGGCGGCTATAACGCATAAAGTCAAAGTCCTTCATCGCCAACTCCGGCAATTTCCACCAACt                                                                                                         >  2:121706/1‑143 (MQ=255)
                                      ccgcccAGACCGATAATCAGCACCTGGCTGTCGAGCAGTTTTTGCTGCCCGTCCAGAGCGATATCGTCGAGCAGGATTTGGCGGCTATAACGCATAAAGTCAAGGTCATTCATCGCCAACTCCTGCAATTTCCAGCAACTGTGCCGTTg                                                                                                >  1:369058/1‑149 (MQ=255)
                                      ccgcccAGACCGATAATCAGCACCTGGCTGTCGAGCAGTTTTTGCTGCCCGTCCAGAGCGATATCGTCGAGCAGGATTTGGCGGCTATAACGCATAAAGTCAAGGTCATTCATCGCCAACTCCTGCAAGTTCCAGCACCTGTGCCGTTg                                                                                                >  1:249714/1‑149 (MQ=255)
                                           cAGGCCGCTAATCACCACCTGTCTGTCGAGCAGTTTTTGCTGCCCGTCCAGAGCGATATCGTCGAGCAGGATTTGGCGGCTATAACGCATATAGTCAAGGTCATTCATCGCCAACTCCTGCAATTTCCAGCATCTGTGCCGtt                                                                                                 <  2:151688/143‑1 (MQ=255)
                                           cAGACCGATAATCAGCACCTGGCTGTCGAGCAGTTTTTGCTGCCCGTCCAGAGCGATATCGTCGAGCAGGATTTGGCGGCTATAACGCATAAAGTCAAGGTCATTCATCGCCAACTCCTGCAATTTCCAGCAACTGTGCCGtt                                                                                                 <  2:727788/143‑1 (MQ=255)
                                              aCCGATAATCAGCACCTGGCTGTCGAGCAGTTTTTGCTGCCCGTCCAGAGCGATATCGTCGAGCAGGATTTGGCGGCTATAACGCATAAAGTCAAGGTCATTCATCGCCAACTCCTGCAATTTCCAGCAACTGTGCCGTTGCCCAACGc                                                                                        >  1:552264/1‑149 (MQ=255)
                                               ccGATAATCAGCACCTGGCTGTCGAGCAGTTTTTGCTGCCCGTCCAGAGCGATATCGTCGAGCAGGATTTGGCGGCTATAACGCATAAAGTCAAGGTCATTCATCGCCAACTCCTGCAATTTCCAGCAACTGTGCCGTTGCCa                                                                                             <  2:688563/143‑1 (MQ=255)
                                                 gATAATCAGCACCTGGCTGTCGAGCATTTTTTGCTGCCCGTCCAGAGCGATATCGTCGAGCAGGATTTGGCGGCTATAACGCATAAAGTCAAGGTCATTCATCGCCAACTCCTGCAATTTCCAGCAACTGTGCCGTTGCCaaa                                                                                           <  2:174332/143‑1 (MQ=255)
                                                 gATAAGCAGCGCCTGGCTGGCGAGCAGTTTTTGCTGCCCGTCCAGAGCGATATCGTCGAGCAGGATTTGGCGGCTATAACGCATAAAGTCAAGGTCATTCATCGCCAACTCCTGCAATTTCCAGCAACTGTGCCGTTGCCaaa                                                                                           <  2:621926/143‑1 (MQ=255)
                                                  tgaaTCTGTACCTGGTTGTCGACCAGTTTTTGCTGCCCGTCCAGAGCGATATCGTCGAGCAGGATTTGGCGGCTATAACGCATAAAGCCAAGGCCAGTCATCGCCAACTCCTGCAATTTCCAGCAACTGTGCCGTTGCCAAAc                                                                                          <  2:323191/141‑1 (MQ=255)
                                                     aTCAGCACCTGGCTGTCGAGCAGTTTTTGCCGAGAGTATACAGCGATATCGTCGAGCAGGATTTGGCGGCTATAACGCATAAAGTCAAGGTCATTCATCGCCAACTCCGGCAAGTTCCAGCAACTGTGGCGTTGCCAAACGCCAGTCTg                                                                                 >  1:448793/1‑149 (MQ=255)
                                                       cAGCACCTGGCTGTCGAGCAGTTTTTGCTGCCCGTCCAGAGCGATATCGTCGAGCAGGATTTGGCGGCTATAACGCATAAAGTCAAGGTCATTCATCGCCAACTCCTGCAATTTCCAGCAACTGTGCCGTTGCCAAACGCCAGTCTGCg                                                                               >  1:661360/1‑149 (MQ=255)
                                                       cAGCACCTGGCTGTAGAGCAGTTTTTGCTGCCCGTCTAGAGCCATATCGTCGAGCAGGATTTGGCGGCTATAACGCATAAAGTCAAGGTCATTCATCGCCAACTCCTGCAATTTCCAGCAACTGTGCCGTTGCCACACGCCAGTCTGCg                                                                               <  1:647237/149‑1 (MQ=255)
                                                           cccgggCTGTCGAGCCGTTTTCGCTGCCCGTCCAGAGCGCTCTCGCCGAGCAGGATTTGGCGGCTATAACTCAGAACGCAAGGGTCATGCTTCGCCAACTCCTGCAATTTCCAGCAACTGTGCCGTTGCCAAACGCCAGTCTGCGGCtt                                                                           <  1:217587/145‑1 (MQ=255)
                                                            ccTGGCTGGCGAGCAGTTTTTGCTGCCCGTCCAGAGCGATATCGTCGAGCAGGATTTGGCGGCTATAACGCATAAAGTCAAGGTCATTCATCGCCAACTCCTGCAATTTCCAGCAACTGTGCCGTTGCCAAACGCCAGTCTGc                                                                                <  2:522748/143‑1 (MQ=255)
                                                                 cTGTCGAGCAGTTTTTGCTGCCCATCCAGAGCGATATCGTCGAGCAGGATTTGGCGGCTATAACGCATAAAGTCAAGGTCATTCATCGCCAACTCCTGCAATTTCCAGCAACTGTGCCGTTGCCAAACGCCAGTCTGCGGCtt                                                                           <  2:526590/143‑1 (MQ=255)
                                                                       aGCAGTTTTTGCTGCCCGTCCAGAGCGATATCGTCGAGCAGGATTTGGCGGCTATAACGCATAAAGTCAAGGTCATTCATCGCCAACTCCTGCAATTTCCAGCAACTGTGCCGTTGCCAAACGCCAGTCTGCGGCTTGAGTaa                                                                     <  2:817540/143‑1 (MQ=255)
                                                                           gTTTTTGCTGCCCGTCCAGAGCGATATCCTCGAGCAGGATTTGGCGGCTATAACGCATAAAGTCAAGGTCATTCATCGCCAACTCCTGCAATTTCCAGCAACTGTGCCGTTGCCAAACGCCAGTCTGCGGCTTGAGTAATGGCGCTgac                                                           <  1:887234/149‑1 (MQ=255)
                                                                            tttttGCTGCCCGTCCAGAGCGATATCGTCGAGCAGGATTTGGCGGCTATAACGCATAAAGTCAAGGTCATTCATCGCCAACCCCTGCAATTTCCAGCAACTGTGCCGTTGCCAAACGCCACTCTGCGGCTTGAGTAATgggg                                                                >  2:471165/1‑141 (MQ=255)
                                                                            tttttGCGGCCCGTCCAGAGCGATATCGTCGAGCAGGATTTGGCGGCTATAACGCATAAAGTCAAGGTCATTCATCGCCAACTCCTGCAATTTCCAGCAACTGTGCCGTTGCCAAACGCCAGTCTGCGGCTTGAGTAATGgcg                                                                <  2:735674/143‑1 (MQ=255)
                                                                                 gctgcCCGTCCAGAGCGATATCGTCGAGCAGGATTTGGCGGCTATAACGCATAAAGTCAAGGTCATTCATCGCCAACTCCTGCAATTTCCAGCAACTGTGCCGTTGCCAAACGCCAGTCTGCGGCTTGAGTAATGGCGCTGACGACGGc                                                     >  1:639882/1‑149 (MQ=255)
                                                                                    gcCCGTCCAGAGCGATATCGTCGAGCAGGATTTGGCGGCTATAACGCATAAAGTCAAGGTCATTCATCGCCAACTCCTGCAATTTCCAGCAACTGTGCCGTTGCCAAACGCCAGTCTGCGGCTTGAGTAATGGCGCTGACGACGGCGAt                                                  <  1:201250/149‑1 (MQ=255)
                                                                                     cccGTCCAGAGCGATATCGTCGAGCAGGATTTGGCGGCTATAACGCATAAAGTCAAGGTCATTCGTCGCCAACTCCTGCAATTTCCAGCAACTGTGCCGTTGCCAAACGCCAGTCTGCGGCTTGAGTAATGGCGCTGacgacg                                                       >  2:313651/1‑143 (MQ=255)
                                                                                     cccGGCCAGAGCGATATCGGCGAGCAGGATTTGGCGGCTATAACGCATAAAGTCAAGGTCATTCATCGCCAACTCCTGCAATTTCCAGCAACTGTGCCGTTGCCAAACGCCAGTCTGCGGCTTGAGTAATGGCGCTGACGACGGCGATa                                                 <  1:125460/149‑1 (MQ=255)
                                                                                      ccGTCCAGAGCGATATCGTCGAGCAGGATTTGGCGGCTATAACGCATAAAGTCACGGTCATTCATCGCCAACTCCTGCAATTTCCAGCAACTGTGCCGTTGCCAAACGCCAGTCTGCGGCTTGAGTAATGGCGCTGACGACgg                                                      <  2:814947/143‑1 (MQ=255)
                                                                                         tCCAGAGCGATATCGTCGAGCAGGATTTGGCGGCTATAACGCATAAAGTCAAGGTCATTCATCGCCAACTCCTGCAATTTCCAGCAACTGTGCCGTTGCCAAACGCCAGTCTGCGGCTTGAGTAATGGCGCTGACGACGGCGATACTGc                                             <  1:692463/149‑1 (MQ=255)
                                                                                           cAGAGCGATATCGTCGAGCAGGATTTGGCGGCTATAACGCATAAAGTCAAGGTCATTCATCGCCAAAACCTGCAATGTCCAGCAACTGTGCCGTTGCCAAACGCCAGTCTGGGGCTTGGGTAATGGCGGTGACGACGGCGATACTGCCg                                           >  1:356510/1‑149 (MQ=255)
                                                                                            agagCGATATCGTCGAGCAGGATTTGGCGACTATAACGCATAAAGTCAAGGTCATTCATCGCCCACTCCTGCAATTTCCAGCAACTGTGCCGTTGCCAAACGCCAGTCTGCGGCTTGAGTAATGGCGCTGACGACGGGGATACTGCCGa                                          >  1:595445/1‑149 (MQ=255)
                                                                                               gCGATATCGTCGAGCAGGATTTGGCGGCTATAACGCATAACGTCAAGGTCATTCATCGCCAACTCCTGCAATTTCCAGCAACTGTGCCGTTGCCAAACGCCAGTCTGCGGCTTGAGTAATGGCGCTGACGACGGCGATACTGCCGacac                                       <  1:739990/149‑1 (MQ=255)
                                                                                               gCGATATCGTCGAGCAGGATTTGGCGGCTATAACGCATAAAGTCAAGGTCATTCATCGCCAACTCCTGCAATTTCCGGCAACTGTGCCGTTGCCAAACGCCAGTCTGTCACGTAATTAATGGGGCTGACGACGGCGATACTGc                                             <  2:380928/143‑1 (MQ=255)
                                                                                                    atCGTCGAGCAGGATTTGGCGGCTATAACGCATAAAGTCAAGGTCATTCATCGCCAACTCCTGCAATTTCCAGCAACTGTGCCGTTGCCAAACGCCAGTCTGCGGCTTGAGTAATGGCGCTGACGACGGCGATACTGCCGACACCCGtt                                  <  1:633433/149‑1 (MQ=255)
                                                                                                       gtcgAGCAGGATTTGGCGGCTATAACGCATAAAGTCAAGGTCATTCATCGCCAACTCCTGCAATTTCCAGCAACTGTGCCGTTGCCAAACGCCAGTCTGCGGCTTGAGTACTGGCGCTGACGCCGGCGATACTGCCGACTCCCGTTGCt                               >  1:410773/1‑149 (MQ=255)
                                                                                                        tcgAGCAGGATTTGGCGGCTATAACGCATAAAGTCAAGGTCATTCATCGCCAACTCCTGCAATTTCCAGCAACTGTGCCGTTGCCAAACGCCAGTCTGCGGCTTGAGTAATGGCGCTGACGACGGCGATACTGCCGACACCCGTTGCTa                              >  1:340589/1‑149 (MQ=255)
                                                                                                           aGCAGGATCTGGCGGCTATAACGCATAAAGTCAAGGTCATTCATCGCCAACTCCTGCAATTTCCAGCAACTGTGCCGTTGCCATCTTTGATTCTGCGGCTTGTCGAATGGCGCTGACGACGGCGATACTGCCGACACCCGTTGCTATCa                           <  1:218438/149‑1 (MQ=255)
                                                                                                             cAGCATTTGGCGGCTATAACGCATAAAGTCAAGGTCATTCATCGCCACCTCCTGCAATTTCCAGCAACTGTGCCGTTGCCAACCGCCAGTCTGCGGCTTGAGTAATGGCGCTGACGACGGCGATACTGCCGACACCCGTTGCt                               <  2:657909/143‑1 (MQ=255)
                                                                                                              aGGATTTGGCGGCTATAACGTATAAAGTCAAGGTGTTTCATCGCCAACTCCTGCAATTTCCAGCAGCTGTGCCGTTGCCAAACGCCAGTCTGCGGCTTGAGTAATGGCGCTGACGACGGCTATATTGCCGACACCCGTTGCTa                              <  2:357095/143‑1 (MQ=255)
                                                                                                                gATTTGGCGGCTATAACGCATAAAGTCAAGGTCATTCATCGCCAACTCCTGCAATTTCCAGCAACTGTGCCGTTGCCAAACGCCAGTCTGCGGCTTGAGTAATGGCGCTGACGACGGCGATACTGCCGACACCCGTTGCTATc                            <  2:226413/143‑1 (MQ=255)
                                                                                                                      gcggcTATAACGCATAAAGTCAAGGTCATTCATCGCCAACTCCTGCAATTTCCAGCGACTGTGCCGTTGCCAAACGCCAGTCTGCGGCTTGAGAAATGGCGCTGACGACGGCGATACTGCGGACCCCCGGTGCTATCACCGCa                      >  2:541446/1‑143 (MQ=255)
                                                                                                                        ggcTATAACGCATAAAGTCAAGGTCATTCATCGCCAACTCCTGCAATTTCCAGCAACTGTGTCGTTGCCAAACGCCAGTCTGCGGCTTGAGTAATGGCGCTGACGACGGCGATACTGCCGACACCCGTTGCTATCACCGCAGGcgcgcg              >  1:865552/1‑149 (MQ=255)
                                                                                                                             taACGCATAAAGTCAAGGTCATTCATCGCCAACTCCTGCAATTTCCAGCAACTGTGCCGTTGCCAAACGCCAGTCTGCGGCTTGAGTAATGGCGCTGACGACGGCGATACTGCCGACACCCGTTGCTATCACCGCAGGCGCGCGTGCCa         >  1:49461/1‑149 (MQ=255)
                                                                                                                                 gCATAAAGTCAAGGTCATTCATCGCCAACTCCTGCAATTTCCAGCAACTGTGCCGTTGCCAAACGCCAGTCTGCGGCTTGAGTAATGGCGCTGACGACGGCGATACTGCCGACACCCGTTGCTATCCCCGCAGGCGCGCGTGCCAGACt     >  1:428461/1‑149 (MQ=255)
                                                                                                                                 gCATAAAGTCAAGGTCATTCATCGCCAACTCCTGCAATTTCCAGCAACTGTGCCGTTGCCAAACGCCAGTCTGCGGCTTGAGTAATGGCGCTGACGACGGCGATACTGCCGACACCCGTTGCTATCACCGCAGGCGCGCGTGCCAGACt     <  1:243388/149‑1 (MQ=255)
                                                                                                                                    tAAAGTCAAGGTCATTCCTCGCCAACTCCTGCAATTTCCAGCAACTGTGCCGTTGCCAAACGCCAGTCTGCGGCTTGAGTAATGGCGCTGACGACGGCGATACTGCCGACACCCGTTGCTATCACCGCAGGCGCGCGTGCCAGACTGAt  <  1:647042/149‑1 (MQ=255)
                                                                                                                                    tAAAGTCAAGGTCATTCATCGCCAACTCCTGCAATTTCCAGCAACTGTGCCGTTGCCAAACGCCAGTCTGCGGCTTGAGTAATGGCGCTGACGACGGCGATACTGCCGACACCCGTTGCTCTCACCGCCGGCGCGCGTGCCAGACTGAt  >  1:115996/1‑149 (MQ=255)
                                                                                                                                            |                                                                                                                                            
CAGCGCCCGCCAGGTACAGCGCAGCAGGTGTACCCAGCCCGCCCAGACCGATAATCAGCACCTGGCTGTCGAGCAGTTTTTGCTGCCCGTCCAGAGCGATATCGTCGAGCAGGATTTGGCGGCTATAACGCATAAAGTCACGGTCATTCATCGCCAACTCCTGCAATTTCCAGCAACTGTGCCGTTGCCAAACGCCAGTCTGCGGCTTGAGTAATGGCGCTGACGACGGCGATACTGCCGACACCCGTTGCTATCACCGCAGGCGCGCGTGCCAGACTGAT  >  CP009273/4183354‑4183634

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑