Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP009273	753,013	G→T	E551* (GAA→TAA)	sdhA →	succinate dehydrogenase, flavoprotein subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	753,013	0	G	T	100.0%	141.1 / NA	44	E551* (GAA→TAA)	sdhA	succinate dehydrogenase, flavoprotein subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (17/27);  total (17/27)

AACACCCAGCGCGTTGAGTGCCTGGAACTGGATAACCTGATGGAAACGGCGTATGCAACGGCTGTTTCTGCCAACTTCCGTACCGAAAGCCGTGGCGCGCATAGCCGCTTCGACTTCCCGGATCGTGATGATGAAAACTGGCTGTGCCACTCCCTGTATCTGCCAGAGTCGGAATCCATGACGCGCCGAAGCGTCAACATGGAACCGAAACTGCGCCCGGCATTCCCGCCGAAGATTCGTACTTACTAATGCGGAGACAGGAAAATGAGACTCGAGT  >  CP009273/752881‑753157                                                                                                                                     |                                                                                                                                                 aaCACCCACCGCGTTGAGTGCCTGGAACTGGATAACCTGATGGAAACGGCGTATGCAACGGCTGTTTCTGCCAACTTCCGTACCGAAAGCCGTGGCGCGCATAGCCGCTTCGACTTCCCGGATCGTGATGATTAAAACTGGCTGTGCCa                                                                                                                                  <  1:726590/149‑1 (MQ=255)   cacCCAGCGCGTTGGGTGCCTGGAACTGGATAACCTGATGGAAACGGCGTATGCAACGGCTGTTTCTGCCAACTTCCGTACCGAAAGCCGTGGCGCGCATAGCCGCTTCGACTTCCCGGATCGTGATGATTAAAACTGGCtgt                                                                                                                                      <  2:404712/143‑1 (MQ=255)        cgcgcGTTGAGTGCCTGGAACTGGATAACCTGATGGAAACGGCGTATGCAACGGCTGTTTCTGCCAACTTCCGTACCGAAAGCCGTGGCGCGCATAGCCGCTTCGACTTCCCGGATCGTGATGATTAAAACTGGCTGTGCCACTCCCTg                                                                                                                           <  1:278388/148‑1 (MQ=255)                  gTGCCTGGAACTGGATAACCTGATGGAAACGGCGTATGCAACGGCTGTTTCTGCCAACTTCCGTACCGAAAGCCGTGGCGCGCATAGCCGCTTCGCCTTCCCGGATCGAGAGGATTACAACTGGCTGTGCCACTCCCTGTATc                                                                                                                       >  2:225107/1‑143 (MQ=255)                       ttgAACTGGAAAACCTGATGGAAACGGCGTATGCAACGGCTGTTTCTGCCAACTTCCGTACCGAAAGCCGTGGCGCGCATAGCCGCTTCGACTTCCCGGATCGTGATGATTAAAACTGGCTGTGCCACTCCCTGTATCTGCCa                                                                                                                  <  2:230877/141‑1 (MQ=255)                       tGGAACTGGATAACCTGATGGAAACGGCGTATGCAACGGCTGTTTCTGCCAACTTCCGTACCGAAAGCCGTGGCGCGCATAGCCGCTTCGACTTCCCGGATCGTGATGATTAAAACTGGCTGTGCCACTCCCTGTATCTGCCAGAGTCg                                                                                                            <  1:110576/149‑1 (MQ=255)                       tGGAACTGGATAACCTGATGGAAACGGCGTATGCAACGGCTGTTTCTGCCAACTTCCGTACCGAAAGCCGTGGCGCGCATAGCCGCTTCGACTTCCCGGATCGTGATGATTAAAACTGGCTGTGCCACTCCCTGTATCTGCCAGAGTCg                                                                                                            <  1:110330/149‑1 (MQ=255)                         gatcTGGATTACCTGCTGGCACCGGCGTATGCAACGGCTGTTTCTGCCAACTTCCGTACCGAAAGCCGTGGCGCGCATAGCCGCTTCGACTTCCCGGATCGTGATGATTAAAACTGGCTGTGCCACTCCCTGTATCTGCCaga                                                                                                                <  2:765920/140‑1 (MQ=255)                             tGGATAACCTGATGGAAACGGCGTATGCAACGGCTGTTTCTGCCAACTTCCGTACCGAAAGCCGTGGCGCGCATAGCCGCTTCGACTTCCCGGATCGTGATGATTAAAACTGGCTGTGCCACTCCCTGTATCTCCCAGAGTCg                                                                                                            >  2:54611/1‑143 (MQ=255)                               gATAACCTGATGGAAACGGCGTATGCAACGGCTGTTTCTGCCAACTTCCGTACCGAGAGCCGTGGCGCGCATAGCCGCTTCGACTTCCCGGATCGTGATGATTAAAACTGGCTGTGCCACTCCCTGTATCTGCCCGAGTCGGAATCCAt                                                                                                    >  1:554724/1‑149 (MQ=255)                                 tAATCTGATGGAAACGGCGTATGCAACGGCTGTTTCTGCCAACTCCCGTACCGAAAGCCGTGGTGCGCATAGCCGCTTCGACTTCCCGTATCGTGATGATTAAAACTGGCTGTGCCACTCCCTGTATCTGCCAGAGTCGGAATCCATGa                                                                                                  <  1:877316/149‑1 (MQ=255)                                    ccTGATGGAAACGGCGTATGCAACGGCTGTTTCTGCCAACTTCCGTACCGAAAGCCGTGGCGCGCATAGCCGCTTCGACTTCCCGGATCGTGATGATTAAAACTGGCTGTGCCACTCCCTGTATCTGCCAGAGTCGGAATCCATGAcgc                                                                                               <  1:384047/149‑1 (MQ=255)                                     cTGATGGAAACGGCGTATGCAACGGCTGTTTCTGCCAACTTCCGTACCGAAAGCCGTGGCGCGCATAGCCGCTTCGACTTCCCGGATCGTGATGATTAAAACTGGCTGTGCCACTCCCTGTATCTGCCAGAGTCGGAATCCAt                                                                                                    >  2:103920/1‑143 (MQ=255)                                      tGATGGAAACGGCGTATGCAACGGCTGTTTCTGCCAACTTCCGTACCGAAAGCCGTGGCGCGCATAGCCGCTTCGACTTCCCGGATCGTGATGATTAAACCTGGCTGTGCCACTCCCTGTATCTGCCAGAGTCGGAATCCCTg                                                                                                   >  2:233081/1‑143 (MQ=255)                                       aagggAAACGGCGTATGCAACGGCTGTTTCCGCCAACTTCCGTACCGACAGCAGGGGCGCGCACAGCCGCTTCTTCTTCCCGGATCGTGATGATTAAAACTGGCTGTGCCACTCCCTGTATCTGCCAGAGTCGGAATCCATGa                                                                                                  <  2:714933/140‑1 (MQ=255)                                            aaaCGGCGTATGCAACGGCTGTTTCTGCCAACTTCCGTACCGAAAGCCGTGGCGCGCATAGCCGCTTCGACTTCCCGGATCGTGATGATTAAAACTGGCTGTGCCCCTCCCTGTATCTGCCAGAGTCGGAATCCATGACGCGCCGAAGc                                                                                       >  1:633323/1‑149 (MQ=255)                                             aaCGGCGTATGCAACGGCTGTTTCTGCCAACTTCCGTACCGAAAGCCGTGGCGCGCATAGCCGCTTCGACTTCCCGGATCGTGATGATTAAAACTGGCTGTGCCACTCCCTGTATCTGCCAGAGGCGGAATCCATGACGGGCCGAAGCg                                                                                      >  1:314175/1‑149 (MQ=255)                                                     aTGCAACGGCTGTTTCTGCCAACTTCCGTACCGAAAGCCGTGGCGCGCATAGCCGCTTCGACTTCCCGGATCGTGATGATTAAAACTGGCTGTGCCACTCCCTGTATCTGCCAGAGTCGGAATCCATGACGCGCCGAAGCGTc                                                                                    <  2:569926/143‑1 (MQ=255)                                                        cAACGGCTGTTTCTGCCAACTTCCGTACCGAAAGCCGTGGCGCGCATAGCCGCTTCGACTTCCCGGATCGTGATGATTAAAACTGGCTGTGCCACTCCCTGTATCTGCCAGAGTCGGAATCCATGACGCGCCGAAGCGTCAAc                                                                                 >  2:413432/1‑143 (MQ=255)                                                         aaCGGCTGTTTCTGCCAACTTCCGTACCGAAAGCCGTGGCGCGCATAGCCGCTTCGACTTCCCGGATCGTGATGATTAAAACTGGCTGTGCCACTCCCTGTATCTGCCAGAGTCGGAATCCATGACGCGCCGAAGCGTCAACATGGAAc                                                                          >  1:718316/1‑149 (MQ=255)                                                           cGGCTGTTTCTGCCAACTTCCGTACCGAAAGCCGTGGCGCGCATAGCCGCTTCGACTTCCCGGATCGTGATGATTAAAACTGGCTGTGCCACTCCCTGTATCTGCCAGAGTCGGAATCCATGACGCGCCGAAGCGTCAACATGGAACCg                                                                        <  1:780351/149‑1 (MQ=255)                                                           cGGCTGTTTCTGCCAACTTCCGTACCGAAAGCCGTGGCGCGCATAGCCGCTTCGACTTCCCGGATCGTGATGATTAAAACTGGCTGTGCCACTCCCTGTATCTGCCAGAGTCGGAATCCAGGACGCGCCGAAGCGTCAACATGGAACCg                                                                        <  1:380310/149‑1 (MQ=255)                                                               tGTTTCTGCCAACTTCCGTACCGAAAGCCGTGGCGCGCATAGCCGCTTCGACTTCCCGGATCGTGATGATTAAAACTGGCTGTGCCACTCCCTGTATCTGCCAGAGTCGGAATCCCTGACGCGCCGAAGCGTCAAAATGGAACCGAAAc                                                                    >  1:348577/1‑149 (MQ=255)                                                               tGTTTCTGCCAACTTCCGTACCGAAAGCCGTGGCGCGCATAGCCGCTTCGACTTCCCGGATCGTGATGATTAAAACTGGCTGTGCCACTCCCTGTATCTGCCAGAGTCGGAATCCATGACGCGCCGAAGCGTCAACATGGAAc                                                                          <  2:288518/143‑1 (MQ=255)                                                                     tGCCAACTTCCGTACCGAAAGCCGTGGCGCGCATAGCCGCTTCGACTTCCCGGATCGTGATGATTAAAACTGGCTGTGCCACTCCCTGTATCTGCCAGAGTCGGAATCCATGACGCGCCGAAGCGTCAACATGGAACCGAAAc                                                                    >  2:660245/1‑143 (MQ=255)                                                                      gcgaaCTTCCGTACCGAAAGCCGTGGCGCGCATAGCCGCTTCGACTTCCCGGATCGTGATGATTAAAACTGGCTGTGCCACTCCCTGTATCTGCCAGAGTCGGAATCCATGACGCGCCGAAGCGTCAACATGGAACCGAAACt                                                                   <  2:447344/140‑1 (MQ=255)                                                                      gCCAACTTCCGTACCGAAAGCCGTGGCGCGCATAGCCGCTTCGACTTCCCGGATCGTGATGATTAAAACTGGCTGTGCCACTCCCTGTATCTGCCAGAGTCGGAATCCATGACGCGCCGAAGCGTCAACATGGGACCGAAACTGCGccc                                                             >  1:292914/1‑149 (MQ=255)                                                                       ccAACTTCCGTACCGAAAGCCGTGGCGCGCATAGCCGCTTCGACTTCCCGGATCGTGATGATTAAAACTGGCTGTGCCACTCCCTGTATCTGCCAGAGTCGGAATCCATGACGCGCCGAAGCGTCAACATGGAACCGAAACTGCGCCCg                                                            <  1:196647/149‑1 (MQ=255)                                                                        cAACTTCCGTACCGAAAGCCGTGGCGCGCATAGCCGCTCCGACTTCCCGGCTCGTGATGATTAAAACTGGCCGTGCCACTCCCTGTATATGCCAGAGTCGGAATCCATGACGCGCCGAAGCGTCAACATGGAACCGAAACTGCGCCCgg                                                           <  1:68108/149‑1 (MQ=255)                                                                                   ccGAAAGCCGTGGCGCGCATAGCCGTTTCGATTTCCCGGTTCGTGATGATTAAAACTGGCTGTGCCACTCCCTGTATCTGCCAGAGTCGGAATCCATGACGCGCCGAAGCGTCAACATGGAACCGAAACTGCGCCCGGCATTc                                                      <  2:220289/143‑1 (MQ=255)                                                                                    cGAAAGCCGTGGCGCGCATAGCCGCTTCGACTTCCCGGATCGTGATGATTAAAACTGGCTGTGCCACTCCCTGTATCTGCCAGAGTCGGAATCCATGACGCGCCGAAGCGTCAACATGGAACCGAAACTGCGCCCGGCCTTCCCGCCGa                                               >  1:168629/1‑149 (MQ=255)                                                                                             tGGCGCGCATAGCCGCTTCGACTTCCCGGATCGTGATGATTAAAACTGGCTGTGCCACTCCCTGTATCTGCCAGAGTCGGAATCCATGACGCGCCGAAGCGTCAACATGGAACCGAAACTGCGCCCGGCATTCCCGCCGAAGATTCGta                                      <  1:381292/149‑1 (MQ=255)                                                                                                cgcgcATAGCCGCTTCGACTTCCCGGATCGTGATGATTAAAACTGGCTGTGCCACTCCCTGTATCTGCCAGAGTCGGAATCCATGACGCGCCGAAGCGTCAACATGGAACCGAAACTGCGCCCGGCATTCCCGCCGAAGATTCGtactt                                   >  1:329812/1‑149 (MQ=255)                                                                                                cgcgcATAGCCGCTTCGACTTCCCGGATCGTGATGATTAAAACTGGCTGTGCCACTCCCTGTATCTGCCAGAGTCGGAATCCATGACGCGCCGAAGCGTCAACATGGAACCGAAACTGCGCCCGGCATTCCCGCCGAAGATTCGtactt                                   <  1:342168/149‑1 (MQ=255)                                                                                                cgcgcATAGCCGCTTCGACTTCCCGGATCGTGATGATTAAAACTGGCTGTGCCACTCCCTGTATCTGCCAGAGTCGGAATCCATGACGCGCCGAAGCGTCAACATGGAACCGAAACTGCGCCCCGCATTCCCGCCGAAGATTCGtactt                                   >  1:329738/1‑149 (MQ=255)                                                                                                 gcgcATAGCCGCTTCGACTTCCCGGATCGTGATGATTAAAACTGGCTGTGCCACTCCCTGTATCTGCCAGAGTCGGAATCCATGACGCGCCGAAGCGTCAACATGGAACCGAAACTGCGCCCGGCATTCCCGCCGAAGATTCg                                        <  2:366322/143‑1 (MQ=255)                                                                                                           gCTTCGACTTCCCGGATCGTGATGATTAAAACTGGCTGTGCCACTCCCTGTATCTGCCAGAGTCGGAATCCATGACGCGCCGAAGCGTCAACATGGAACCGAAACTGCGCCCGGCATTCCCGCCGAAGATTCGTACTTACTaa                              >  2:44641/1‑143 (MQ=255)                                                                                                            cTTCGACTTCCCGGATCGTGATGATTAAAACTGGCTGGGCCACTCCCTGTATCTGCCAGAGTCGGAATCCATGACGCGCCGAAGCGTCAACATGGAACCGAAACTGCGCCCGGCATTCCCGCCGAAGATTCGTACTTACTAATGCGgag                       <  1:734454/149‑1 (MQ=255)                                                                                                                 aCTTCCCGGATCGTGATGATTAAAACTGGCTGTGCCACTCCCTGTATTTGCCAGAGTCGGAATCCATGACGCGCCGAAGCGTCAACATGGAACCGAAACTGCGCCCGGCATTCCCGCCGAAGATTCGTACTTACTAATGCGGAGACAgg                  <  1:157335/149‑1 (MQ=255)                                                                                                                 aCTTCCCGGATCGTGATGATTAAAACTGGCTGTGCCACTCCCTGTATCTGCCAGAGTCGGAATCCATGACGCGCCGAAGCGTCAACATGGAACCGAAACTGCGCCCGGCATTCCCGCCGAAGATTCGTACTTACTAATGCGGAGACAgg                  <  1:782080/149‑1 (MQ=255)                                                                                                                   ttCCCGGATCGTGATGATTAAAACTGGCTGTGCCACTCCCTGTATCTGCCAGAGTCGGAATCCATGACGCGCCGAAGCGTCAACATGGAACCGAAACTGCGCCCGGCATTCCCGCCGAAGATTCGTACTTACTAATGCGgaga                      <  2:221820/143‑1 (MQ=255)                                                                                                                       cGGATCGTGATGATTAAAACTGGCTGTGCCACTCCCTGTATCTGCCAGAGTCGGAATCCATGACGCGCCGAAGCGTCAACATGGAACCGAAACTGCGCCCGGCATTCCCGCCGAAGATTCGTTCTTACTAATGCGGAGACAgg                  >  2:309031/1‑143 (MQ=255)                                                                                                                         gATCGGGATGATTAAAACTGGCTGTGCCACTCCCTGTATCTGCCAGAGTCGGAATCCATGACGCGCCGAAGCGTCAACATGGAACCGAAACTGCGCCCGGCATTCCCGCCGAAGATTCGTACTTACTAATGCGGAGACAGGAAAATgag          <  1:344008/149‑1 (MQ=255)                                                                                                                          aTCGTGATGATTAAAACTGGCTGTGCCACTCCCTGTATCTGCCAGAGTCGGAATCCATGACGCGCCGAAGCGTCAACATGGAACCGAAACTGCGCCCGGCATTCCCGCCGAAGATTCGTACTTACTAATGCGGAGACAGGAAAATgaga         <  1:387004/149‑1 (MQ=255)                                                                                                                              tgatgattaAAACTGGCTGTGCCACTCCCTGTATCTGCCAGAGTCGGAATCCATGACGCGCCGAAGCGTCAACATGGAACCGAAACTGCGCCCGGCATTCCCGCCGAAGATTCGTACTTACTAATGCGGAGACAGGAAAATGAGACTCg     >  1:733534/1‑149 (MQ=255)                                                                                                                                 tgattaAACCTGGCTGTGCCACTCCCTGTATCTGCCAGAGTCGGAATCCATGACGCGCCGAAGCGTCAACATGGAACCGAAACTGCGCCCGGCATTCCCGCCGAAGATTCGTACTTACTAATGCGGAGACAGGAAAATGAGACTCGAGt  <  1:81773/149‑1 (MQ=255)                                                                                                                                     |                                                                                                                                                 AACACCCAGCGCGTTGAGTGCCTGGAACTGGATAACCTGATGGAAACGGCGTATGCAACGGCTGTTTCTGCCAACTTCCGTACCGAAAGCCGTGGCGCGCATAGCCGCTTCGACTTCCCGGATCGTGATGATGAAAACTGGCTGTGCCACTCCCTGTATCTGCCAGAGTCGGAATCCATGACGCGCCGAAGCGTCAACATGGAACCGAAACTGCGCCCGGCATTCCCGCCGAAGATTCGTACTTACTAATGCGGAGACAGGAAAATGAGACTCGAGT  >  CP009273/752881‑753157

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑