Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,719,426 T→C 43.6% intergenic (‑321/+2) kgtP ← / ← rrfG alpha‑ketoglutarate transporter/5S ribosomal RNA of rrnG operon

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,719,4260TC43.6% 46.5 / 127.4 113intergenic (‑321/+2)kgtP/rrfGalpha‑ketoglutarate transporter/5S ribosomal RNA of rrnG operon
Reads supporting (aligned to +/- strand):  ref base T (32/31);  new base C (28/21);  total (60/53)
Fisher's exact test for biased strand distribution p-value = 5.69e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.35e-01

Only 100 of 113 total aligned reads displayed.

TATGGAAGTGGGCAAGTTGGGGAAGCCGTATCCGTTGCTGAATCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACGGCCGCCAGGCAAATTCTGTTTTATCAGACCGC  >  CP009273/2719283‑2719568
                                                                                                                                               |                                                                                                                                              
tATGGAAGTGGGCAAGTTGGGGAAGCCGTATCCGTTGCTGAATCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGCTGGCCTTTCGCATAATTCGATGc                                                                                                                                           >  1:28764/1‑149 (MQ=255)
  tGGTAGTGGGCAAGTGGGGGAAGCCGTATCCGTTGCTGAATCTGGCATATGTAGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGCCAGGATGGCCTTTCGCATGATTTg                                                                                                                                               >  2:761733/1‑143 (MQ=37)
  tGGAAGTGGGCAAGTTGGGGAAGCCGTATCCGTTGCTGAATCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTTGATGCCt                                                                                                                                         <  1:801879/149‑1 (MQ=255)
     aaGTGGGCAAGTTGGGGAAGCCGTATCCGTTGCTGAATCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCTATTGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATACTTCGATg                                                                                                                                            >  2:127661/1‑143 (MQ=255)
      aGTGGGCAAGTTGGGGAAGCCGTATCCGTTGCTGAATCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCa                                                                                                                                     <  1:1063965/149‑1 (MQ=255)
      aGTGGGCAAGTTGGGGAAGCCGTATCCGTTGCTGAATCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCa                                                                                                                                     <  1:717674/149‑1 (MQ=255)
      aGTGGGCAAGTTGGGGAAGCCGTATCCGTTGCTGAATCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCa                                                                                                                                     <  1:854600/149‑1 (MQ=255)
          ggCAAGTTGGGGAGGCCGTATCCGTTGCTGAATCTGGCATATGTGGGAGTATAGGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCCTCCGTCAGGATGGCCTTTCGCATAATGTGCTGCCTgg                                                                                                                                       <  2:1038182/143‑1 (MQ=25)
           gCAAGTTGGGGAAGCCGTATCCGTTGCTGAATCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCCTTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATCGCCTTTCGCATAATTTGATGCCTGGCAGTTcc                                                                                                                                >  1:355652/1‑149 (MQ=255)
           gCAAGTTGGGGAAGCCGTATCCGTTGCTGAATCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTTGATCGCTGGCAGTTcc                                                                                                                                >  1:1030442/1‑149 (MQ=255)
           gCAAGTTGGGGAAGCCGTATCCGTTACTGAATCTGGCATATGTGGGACTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTTGATGCCTGGCAGTTcc                                                                                                                                >  1:731041/1‑149 (MQ=255)
           gCAAGGTGGGGAAGCCGTATCCGTTGCTGAATCTGGCATAGGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTcc                                                                                                                                <  1:459703/149‑1 (MQ=255)
             aaGTTGGGGAGGCCGGCGCCGTTGCTGATTCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTGTTCTGCGCCAATGCAAAAAGGCCCTCCGTCAGGATGGCCTTTCGCATAATTTGATGCCTGGCAg                                                                                                                                    <  2:97795/143‑1 (MQ=21)
                ttGGGGAAGCCGTATCCGTTGCTGAATCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGCCAGTTCcctcct                                                                                                                           >  1:65119/1‑149 (MQ=255)
                     gAAGCCGTATCCGTTGCTGAATCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGc                                                                                                                      >  1:1207989/1‑149 (MQ=255)
                      aaGCCGTATCCGTTGCTGAATCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCCTCCGTCACGATGGCCATGCGCAAAATTTGTTGCCTGGCAGTTCCCTACTCTCGCa                                                                                                                     >  1:426386/1‑149 (MQ=25)
                        gCCGTATCCGTTGCTGAATCTGACATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATATTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTTGATGCCTGGCAGTTCCCTACTCTCGCATg                                                                                                                   >  1:984884/1‑149 (MQ=255)
                          cGTATCCGTTGCTGAATCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTTGATGCCTGGCAGTTCCCTACTCTCGCATggg                                                                                                                 <  1:968288/149‑1 (MQ=255)
                          cGTATCCGTTGCTGAATCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCg                                                                                                                       <  2:913700/143‑1 (MQ=255)
                          cGTATCCGTTGCTGAATCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATggg                                                                                                                 <  1:424278/149‑1 (MQ=255)
                           gTATCCGTTGCTGAATCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTTGATGCCTGGCAGTTCCCTACTCTCGCATgggg                                                                                                                <  1:870184/149‑1 (MQ=255)
                            tATCCGTTGCTGAATCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTTGATGCCTGGCAGTTCCCTACTCTCGCa                                                                                                                     >  2:621105/1‑143 (MQ=255)
                             aTCCGTTGCTGAATCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCCTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCAt                                                                                                                    <  2:288191/143‑1 (MQ=255)
                               ccGTTGCTGAATCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTTGATGCCTGGCAGTTCCCTACTCTCGCATgg                                                                                                                  <  2:836191/143‑1 (MQ=255)
                                 gTTGCTGAATCTGGCATAGGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTTGATGCCTGGCAGTTGCCTACTCTCGCATgggg                                                                                                                >  2:584299/1‑143 (MQ=255)
                                    gccgAATCGGCCATATCTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCTTACGTCATGATGGCCTTTCGCATAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCcac                                                                                                       <  1:39040/146‑1 (MQ=25)
                                    gCTGAATCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTTGATGCCTGGCAGTTCGCTACTCTCGCATGGGGAGACCCcac                                                                                                       >  1:790923/1‑149 (MQ=255)
                                    gCTGAATCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCcac                                                                                                       >  1:516683/1‑149 (MQ=255)
                                     cTGAATCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCcaca                                                                                                      >  1:22762/1‑149 (MQ=255)
                                       ttccgatctatatGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACt                                                                                                    <  1:348436/140‑1 (MQ=255)
                                       gAATCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAACTTCATGCGGAGGGGTTCCCTACTCTCGCATGGGGAGACCCCACCCt                                                                                                    >  1:679903/1‑149 (MQ=25)
                                        aaTCTGGCCTACGCGGGAGCAGAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTa                                                                                                   <  1:306264/149‑1 (MQ=25)
                                        aaTCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACGCTa                                                                                                   >  1:1101484/1‑149 (MQ=255)
                                        aaTCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACtc                                                                                                   >  1:1101512/1‑148 (MQ=255)
                                        aaTCTGGCATATGTGGGAGTAGAAGACGCGCAGCGTCGCCTCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTa                                                                                                   <  1:1087895/149‑1 (MQ=255)
                                          tCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAGTGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTTGATGCCTGGCAGTTCCCTACTCTCGCCTGGGGAGACCCCACACTAcc                                                                                                 >  1:276856/1‑149 (MQ=255)
                                            tGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGGTGGCCTTTCGCATACTTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCCCACTACCAt                                                                                               >  1:910971/1‑149 (MQ=255)
                                                atatGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACACCACACTAcc                                                                                                 >  2:1147185/1‑143 (MQ=255)
                                                atatGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTTGAGGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACCCTACCATCGgc                                                                                           >  1:688757/1‑149 (MQ=255)
                                                 tatGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTaaga                                                                                                >  2:302753/1‑140 (MQ=255)
                                                  atGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTcccgt                                                                                               >  2:287327/1‑141 (MQ=255)
                                                     ttggAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTAc                                                                                      <  1:1265549/147‑1 (MQ=255)
                                                     tgGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACGCCACACTACCATCGGCGCTAc                                                                                      >  1:826018/1‑149 (MQ=255)
                                                     tgGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCACCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACGCCACACTACCATCGGCGCTAc                                                                                      >  1:903756/1‑149 (MQ=255)
                                                           tataAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAGAGGCCCCGAGTGGTGAGGACCTTTGGGAGAAGTTGATGCCTGGCAGTTCCCTCCTCTCGCATGGGGAGACCCCACACTACCATCGGCGGTGCGGCGtt                                                                                >  1:308495/1‑149 (MQ=18)
                                                              aaGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCa                                                                             >  1:474490/1‑149 (MQ=255)
                                                               agtcgcgCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCAc                                                                            <  1:209966/146‑1 (MQ=255)
                                                               aGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCg                                                                                  <  2:1022370/143‑1 (MQ=255)
                                                                gACGCGCCGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATACGGAGCACCCACACTACCATCGTCGCTCCGGcct                                                                                 >  2:1109558/1‑141 (MQ=21)
                                                                gACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGt                                                                                 >  2:622881/1‑143 (MQ=255)
                                                                   gctcAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAAGCCATCCGTCAGGATGTCCTTTCTCCTAATGCGATGCCTGGCAATTCCCTCCTCTCGCATGGGGTGAGCCCAAACTCCCATCGCCGCTACGGCGTTTc                                                                              >  2:316263/4‑143 (MQ=18)
                                                                     gccgCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGa                                                                      <  1:30137/146‑1 (MQ=255)
                                                                     gcAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGa                                                                      <  1:1216640/149‑1 (MQ=255)
                                                                       cgcggcgCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGt                                                                    <  1:432303/148‑1 (MQ=255)
                                                                         cggcGCAGCAGGCATTTTTTGCTGCGCCAATGCAAAAAGGCCATCCGTCAGGAGGGCCTGTCGCATAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTc                                                                  <  1:189654/146‑1 (MQ=21)
                                                                                tcgggCCTTGTTTTCTGCGACAATGCAAAAAGGCCATCCGTCAGGATGGCGCTTCGCATAATGCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACTATCGGCGCTACGGCGTTTCACTTCTGAGTTCg                                                                 <  2:616317/140‑1 (MQ=18)
                                                                                tCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTCCGGCGTTTCACTTCTGAGTTCGGCATgg                                                           >  1:1053643/1‑149 (MQ=255)
                                                                                 gaGGGATTTTTTTCTGCGCCAATGCAAAAAGGCCACCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCgg                                                                <  2:55269/142‑1 (MQ=255)
                                                                                 cAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATggg                                                          <  1:429561/149‑1 (MQ=255)
                                                                                      aTTTTGTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAg                                                     <  1:235442/149‑1 (MQ=255)
                                                                                          ttttCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTgg                                                 >  1:1143677/1‑149 (MQ=255)
                                                                                          ttttCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTgg                                                 >  1:620438/1‑149 (MQ=255)
                                                                                           tttCTGCGGCAATGCAATAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTCCTCTCGCATGGGGAGACCCCACACTTCCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCa                                                      <  2:460226/143‑1 (MQ=18)
                                                                                            ttCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAg                                                     <  2:893253/143‑1 (MQ=255)
                                                                                            ttCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAg                                                     <  2:791700/143‑1 (MQ=255)
                                                                                            ttCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCACGGGGTCAg                                                     >  2:1072151/1‑143 (MQ=255)
                                                                                            ttCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGGTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGa                                               >  1:735360/1‑149 (MQ=255)
                                                                                               tGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATGATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTCCCATCGGCGCTACGGCGTTTCGCTTCTGAGTGCGGCAAGGGGTGAGGTg                                                  >  2:334977/1‑143 (MQ=18)
                                                                                                   ccAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACcgc                                        >  1:165133/1‑149 (MQ=255)
                                                                                                    cAATGCAAAAAGGCCATCCGTCAGGCTTGCCTTTCGCACAATTCGATTCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGacc                                             <  2:61588/143‑1 (MQ=17)
                                                                                                       tGCAAAAAGGCCATCCGTCAGGATGACCTTTCGCATAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGCGTTCGCCATGGGGTCAGGTGGGACCACCGCGCTa                                    >  1:655519/1‑149 (MQ=255)
                                                                                                        gCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGCTGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTGTGAATTCGGCCTGGGGTCAGGTGGGACCACCGCGCTAc                                   >  1:353783/1‑149 (MQ=17)
                                                                                                         cAAAAAGGCCATCCGTTCGGATGGCCTTTCGCATAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGCCCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACg                                  <  1:562896/149‑1 (MQ=255)
                                                                                                          aaaaaGGCCATTCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACcgcg                                       <  2:730397/143‑1 (MQ=255)
                                                                                                              cggCCATCGGTCAGGGTGGCCTGTCGCACAATTGGATGCCGGGCAGTGCCCTACTCCCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACGgccgc                             <  1:95139/148‑1 (MQ=14)
                                                                                                               ggccatcctgacggATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTCCGTAAGTCATCGTGTCAGGTGGGACCACCGCGCTACGgccgcc                            >  1:1003411/13‑149 (MQ=14)
                                                                                                                gCCATCCGTCAGGATGGCCTTTCGCATAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACGGCCGCCa                           <  1:798316/149‑1 (MQ=255)
                                                                                                                 ccATCCGTCAGGATGGCCTTTCGCATAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTCCGGCCGCCAg                          >  1:1277177/1‑149 (MQ=255)
                                                                                                                    tCCGTCAGGATGGCCTTTCGCATAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCGACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACGcccgc                             >  2:850992/1‑143 (MQ=255)
                                                                                                                       gTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACGGCCGCCAg                          >  2:836002/1‑143 (MQ=255)
                                                                                                                        tCAGGATGGCCTTTCGCATAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCATTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACGGCCGCCAGGCAAAtt                   <  1:842524/149‑1 (MQ=255)
                                                                                                                           ggATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACGGCCGCCAGGCaa                      >  2:900448/1‑143 (MQ=255)
                                                                                                                            gattcccTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACGGCCGCCAGGCAAATTCTGt               <  1:293027/144‑1 (MQ=255)
                                                                                                                             aTGGCTTTTCGCATATATCGATCTCTCGCAGTACCCTACTCTCGCATGCGGAGACCCCACACTACCAGCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACGGCCGCCAGGCAAATTCTGtt              <  1:997490/149‑1 (MQ=11)
                                                                                                                             aTGGCCTTTCGCATAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGGGTTCGGCATGGGGTCAGGTGGGATCACCGCGCTACGGCCGCCAGGTCAATTCTGtt              >  1:121146/1‑149 (MQ=255)
                                                                                                                             aTGGCCTTTCGCATAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACGGCCGCCAGGCAAATTCTGtt              <  1:1227463/149‑1 (MQ=40)
                                                                                                                                 ccTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACGGCCGCCAGGCAAATCCTg                >  2:902385/1‑143 (MQ=255)
                                                                                                                                 ccTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACGGCCGCCAGGCAAAGTCTg                >  2:902465/1‑143 (MQ=255)
                                                                                                                                 ccTCTCACATAATTTGAGGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACGGCCGCCAGGCAAATTCTGTTTTAt          <  1:361585/149‑1 (MQ=12)
                                                                                                                                   tttCGCATAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACGGCCGCCAGGCAAATTCGGTTTTGTcc        >  1:77307/1‑148 (MQ=25)
                                                                                                                                   ttccGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACGGCCGCCAGGCAAATTCTGtt              <  2:307307/140‑1 (MQ=17)
                                                                                                                                    ttCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACGGCCGCCAGGCACATTCTGttt             >  2:570723/1‑143 (MQ=21)
                                                                                                                                       gCATAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACGGCCGCCAGGCAAATTCTGTTTTATCAGAcc    >  1:860641/1‑149 (MQ=32)
                                                                                                                                       gCATAATTTGACGCCTGGCAGTTCCCTACTCTCGCATGGGGCGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACGGCCGCCAGGCAAATTCTGTTTTAt          <  2:740078/143‑1 (MQ=17)
                                                                                                                                         aTAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACGGCCGCCAGGCAAATTCTGTTTTATCAGACCGc  >  1:443574/1‑149 (MQ=11)
                                                                                                                                         aTAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACGGCCGCCAGGCAAATTCTGTTTTATCa        >  2:1151243/1‑143 (MQ=11)
                                                                                                                                         aTAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACGGCCGCCAGGCAAATTCTGTTTTATCa        >  2:337583/1‑143 (MQ=11)
                                                                                                                                         aTAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACGGCCGCCAGGCAAATTCTGTTTTATCa        >  2:97967/1‑143 (MQ=11)
                                                                                                                                         aTAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACGGCCGCCAGGCAAATTCTGTTTTATCAGACCGc  >  1:1209946/1‑149 (MQ=11)
                                                                                                                                         aTAATCTGCTTCCTGGCAGTTCCCTACTTTCGCATGGGGAGACCCCACTCTACCATCGGCGCTACGGCGTTTCACTTCTGAGTGCGGCATGGGGTCAGATGGGACCACCGCGCTACGGCCTCCAGGCAGATTCTGttttttga        >  2:751364/1‑139 (MQ=11)
                                                                                                                                               |                                                                                                                                              
TATGGAAGTGGGCAAGTTGGGGAAGCCGTATCCGTTGCTGAATCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACGGCCGCCAGGCAAATTCTGTTTTATCAGACCGC  >  CP009273/2719283‑2719568

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: