Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,718,678 C→G 30.7% G143A (GGC→GCC)  kgtP ← alpha‑ketoglutarate transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,718,6780CG30.7% 156.8 / 94.7 110G143A (GGC→GCC) kgtPalpha‑ketoglutarate transporter
Reads supporting (aligned to +/- strand):  ref base C (42/32);  new base G (15/18);  total (58/52)
Fisher's exact test for biased strand distribution p-value = 3.01e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.67e-01

Only 100 of 112 total aligned reads displayed.

CAGCGTCTTCCATGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCCGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCGGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACACATCATACACACCGATAACAGCATCGATTT  >  CP009273/2718535‑2718826
                                                                                                                                               |                                                                                                                                                    
cAGCGTCTTCCATGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCCGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGGCatat                                                                                                                                                 >  1:1052073/1‑149 (MQ=255)
   cGTCTTCCATGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCCGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGGCa                                                                                                                                                    >  2:699916/1‑143 (MQ=255)
    ttCTTCCATGGTCTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCCGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGGCat                                                                                                                                                   <  2:889911/142‑1 (MQ=255)
    gTCTGCCATGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCCGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGGCat                                                                                                                                                   <  2:882630/143‑1 (MQ=255)
      cTTCCATGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCCGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCCg                                                                                                                                           <  1:1026646/149‑1 (MQ=255)
      cTTCCATGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCCGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCCg                                                                                                                                           >  1:340818/1‑149 (MQ=255)
       ttCCATGGGGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCCGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGGCATAtt                                                                                                                                                <  2:120652/143‑1 (MQ=255)
         ccATGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCCGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTCCAACGCCAACTTCACCCATATAGGTGGCGCTGGTGCCCTATtcc                                                                                                                                              >  2:104283/1‑142 (MQ=255)
          cATGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCCGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGGCATATtctc                                                                                                                                             <  2:425515/143‑1 (MQ=255)
          cATGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCCGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGGCATATtctc                                                                                                                                             <  2:828955/143‑1 (MQ=255)
          cATGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCCGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGGCATATTCTCCGCCaa                                                                                                                                       <  1:856228/149‑1 (MQ=255)
          cATGGTGTGTTGTAAAACCACGACAAACAGTAGGGCTAGCAGTTGTCCGCCGATCAACGTGACATACTGAACGGATGCGTAAAAACCTTTGCGCCCTTCAACGGCACGTTCACTCATATAGGTGGCACTGGTGCCATATTCTCCGCCac                                                                                                                                       >  1:370363/1‑148 (MQ=255)
            tGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCCGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCCGCCAACa                                                                                                                                     >  1:1178188/1‑149 (MQ=255)
            gggTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCCGCCGGTCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCCg                                                                                                                                           <  2:677514/142‑1 (MQ=255)
              gttggttgtAAAACCACGAGAACCAGTAGGGCTAGCAGTTGTCCGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCTTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGTCATATTCTCCGCCAACAGa                                                                                                                                   <  1:631670/145‑1 (MQ=255)
              gtgtgtTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCCGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCGGGTGCCCTATTCTCCGcc                                                                                                                                         >  2:219649/1‑143 (MQ=255)
                gtgtTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCCGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCCGCCAACAGATa                                                                                                                                 <  1:970310/149‑1 (MQ=255)
                  gttgtAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCCGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCCGCCAACa                                                                                                                                     >  2:960271/1‑143 (MQ=255)
                      tAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCTGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGGCATATTCTCCGCCAACAGATAATCCCt                                                                                                                           <  1:1111530/149‑1 (MQ=255)
                      tAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCCGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCt                                                                                                                           >  1:1159982/1‑149 (MQ=255)
                        aaaCCACGAAAACCAGTAGGGCTAGCAGTTGTCCGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGa                                                                                                                         <  1:938521/149‑1 (MQ=255)
                         aaCCACGACAACCAGTAGGGCTAGCAGTTGTCCGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCACCGGCAACTTCACTCATATACGGGGCGCTGGTGGCATATTCTCCGCCAACAGATAATc                                                                                                                              >  2:936517/1‑143 (MQ=255)
                          cccACGACAACCAGTAGGGCTAGCAGTTGTCCGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGGCATATTCTCCGCCAACAGATAATCCCTGaaa                                                                                                                       <  1:1266525/148‑1 (MQ=255)
                            cACGACAACCAGTAGGGCTAGCAGTTGTCCGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCCCCGCCAACAGATAATCCCt                                                                                                                           >  2:970588/1‑143 (MQ=255)
                              cGACAACCAGTAGGGCTAGCAGTTGTCCGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTgc                                                                                                                         >  2:912491/1‑142 (MQ=255)
                              cGACAACCAGTAGGGCTAGCAGTTGTCCGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGa                                                                                                                         >  2:497632/1‑143 (MQ=255)
                              cGACAACCAGGAGGGCTAGCAGTTGTCCGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATaaa                                                                                                                   <  1:68253/149‑1 (MQ=255)
                               gACAACCAGTAGGGCTAGCAGTTGTCCGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAAc                                                                                                                  <  1:1091865/149‑1 (MQ=255)
                                aCAACCAGTAGGGCTAGCAGTTGTGCGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAAcg                                                                                                                 >  1:968289/1‑149 (MQ=255)
                                     cAGTAGGGCTAGCAGTTGTCCGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATGAAagagcaa                                                                                                            >  1:673337/1‑147 (MQ=255)
                                     cAGTAGGGCTAGCAGTTGTCCGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAAcgagcga                                                                                                            >  1:141392/1‑149 (MQ=255)
                                     cAGTAGGGCTAGCAGTTGTCCGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCGGAAATAAAc                                                                                                                  >  2:493567/1‑143 (MQ=255)
                                     cAGTAGGGCTAGCAGTTGTCCGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCGTTGCGCCCTTCAACGGCAACTTCACTCATATAGATAGCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATCAAc                                                                                                                  >  2:618327/1‑143 (MQ=255)
                                         aGGGCTAGCAGTTGTCCGCCGATCAACGGCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGGCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAg                                                                                                        <  1:359789/149‑1 (MQ=255)
                                           ggCTAGCAGTTGTCCGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGGCATATTCTCCGCCAACAGATAATCCCTGAAATAAAcgagcga                                                                                                            <  2:989247/143‑1 (MQ=255)
                                           ggCTAGCAGTTGTCCGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCCGCCAACAGATAACCCCTGAAATAAACTAGCGAGAAGCa                                                                                                      >  1:404106/1‑149 (MQ=255)
                                           ggCTAGCAGTTGTCCGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGACAACTTCACTCATATAGGGGGCGCTGGTGGCATATTCTCCGCCAACAGATAATCCCTGAAATACAcgagcga                                                                                                            >  2:643929/1‑143 (MQ=255)
                                            gCTAGCAGTTGTCCGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCaa                                                                                                     <  1:1147301/149‑1 (MQ=255)
                                                gCAGTTGTCCGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGGCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGc                                                                                                       >  2:277306/1‑143 (MQ=255)
                                                 tggTTGTCCGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCa                                                                                                      >  2:756592/3‑143 (MQ=255)
                                                  aGTTGTCCGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCGTTGCGCCCTTCAACGTCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCAGCCAACAGATAATCCCTGAAATAAACAAGCGAGAAGCaa                                                                                                     >  2:406643/1‑143 (MQ=255)
                                                    ttGTCCGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCg                                                                                             >  1:155883/1‑149 (MQ=255)
                                                    ttGTCCGCCGAGCAANGTCACATACTGACATGATGCGTAAAACCCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCg                                                                                             <  1:1195142/149‑1 (MQ=255)
                                                      gTCCGCCGATCAACGTAACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGGCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCGGa                                                                                           <  1:352682/149‑1 (MQ=255)
                                                       tCCGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCGGAg                                                                                          >  1:1257669/1‑149 (MQ=255)
                                                       tCCGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCGGAg                                                                                          >  1:1257705/1‑149 (MQ=255)
                                                        ccgccgATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGTGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCGGAGc                                                                                         <  1:905196/149‑1 (MQ=255)
                                                        ccgccgATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGc                                                                                               <  2:437206/143‑1 (MQ=255)
                                                         cgccgATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGcc                                                                                              <  2:672042/143‑1 (MQ=255)
                                                             gATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCCGCCAACAGATAAGCCCTGAAATAAACGAGCGAGAAGCAATAATGCCGGAg                                                                                          <  2:97852/143‑1 (MQ=255)
                                                             gATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAACTGGCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCGGAg                                                                                          >  2:357564/1‑143 (MQ=255)
                                                              aTCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGGCATGTTCTCCGCCAACAGATAATCCCTGAAATAAACGATCGAGAAGCAATAAACCCGCAGc                                                                                         >  2:825751/1‑143 (MQ=255)
                                                              aTCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGGCATATTCTCCCCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAAAAATCCCGGAGc                                                                                         >  2:872824/1‑143 (MQ=255)
                                                               tCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCGGAGcc                                                                                        <  2:610726/143‑1 (MQ=255)
                                                                     tCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCCGCCAACAGATAACCCCTGAAATAAACGAGCGAGAAGCAATAATGCCGGGGCCCACGTACCtatg                                                                            >  1:388000/1‑148 (MQ=255)
                                                                     tCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATACAGGTGGCGCTGGTGGCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCGGAGCCCACGTACCtata                                                                            >  1:866167/1‑149 (MQ=255)
                                                                      cacaTACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGGCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCGGAGCCCACGTACCTATAg                                                                           <  1:892933/149‑1 (MQ=255)
                                                                       acaTACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCTACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCGGAGCCCACGTACCTATAGt                                                                          <  1:916445/149‑1 (MQ=255)
                                                                             tGAAATGATGCGTAAAAACCTTTGCGCCCTTCAATGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGACAAGCAATAATGCCGGAGCCCACGTACCTATAGt                                                                          >  2:895065/1‑143 (MQ=255)
                                                                               aaaTGATGCGTAAAAACATTTGTGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGACATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCGGAGCCCACGTACCTATAGttt                                                                        >  2:1063403/1‑143 (MQ=255)
                                                                                aaTGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCGGAGCCCACGTACCTATAGTTTc                                                                       <  2:884862/143‑1 (MQ=255)
                                                                                 atgatgCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCGGAGCCCACGTACCTATAGTTTCATAACCt                                                                <  1:163634/149‑1 (MQ=255)
                                                                                   gatgCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCGGAGCCCACGTACCTATAGTTTCATa                                                                    >  2:1015530/1‑143 (MQ=255)
                                                                                    atgCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCGGAGCCCACGTACCTATAGTTTCATAACCTggg                                                             <  1:262868/149‑1 (MQ=255)
                                                                                      gCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCGGAGCCCACGTACCTATAGTTTCATAACCTGGGag                                                           >  1:811996/1‑149 (MQ=255)
                                                                                        gTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGGCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCGGAGCCCACGTACCTATAGTTTCATAACCTGGGaggc                                                         >  1:221827/1‑149 (MQ=255)
                                                                                         tAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGGCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCGGAGCCCACGTACCTATAGTTTCATAACCTGGGaggca                                                        <  1:937436/149‑1 (MQ=255)
                                                                                         tAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCGGAGCCCACGTACCTATAGTTTCATAACCTGGGaggca                                                        >  1:1227123/1‑149 (MQ=255)
                                                                                          aaaaaCCGTTGCGCCCGTCAACGGCACCTTCACTCATATCGTTGGCGCTGGTGCCATATTCTCCGCCAACAGATAAGCCCTGAAATAAACGAGCGAGAAGCAATAATGCCGGAGCCCACGTACCTATAGTTTCATAACCTggg                                                             <  2:738299/143‑1 (MQ=255)
                                                                                           aaaaCCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGGCATATTCTCCGCCAACAGATAATCCCTGAAATAACCGAGCGAGAAGCAATAATGCCGGCGCCCACGTACATATAGTTTCATAACCTGGGa                                                            >  2:1050809/1‑143 (MQ=255)
                                                                                            ttcctgatGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGGGCCATATTCTCCGCCCACAGATAATCCCTGAAATAAACGAGCGCGAAGCAATAATGCCGCAGCCGACGTACCCATAGTTTCATAACCTggggg                                                           >  2:474104/8‑141 (MQ=255)
                                                                                            aaaCCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCGGAGCCCACGTACCTATAGTTTCATAACCTGGGaggcaggc                                                     <  1:212332/149‑1 (MQ=255)
                                                                                               ccTTCGCCCCCTTCAACGGCAACTTCCCTCATCTAGGGGGCGCTGGTGGCATATTCTCCGCCAACAGATAATCCCTGAAAGAAACGAGCGAGAAGCAATAATGCCGGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGAt                                                  <  1:615919/149‑1 (MQ=255)
                                                                                                   tGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCGGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAAcc                                              >  1:806902/1‑149 (MQ=255)
                                                                                                   gccgcCCTGCAACGGCAACTTCACTCATCTTTGGGGTTCTTGTGCCATATTCTCAGCCAACAGATAATCCCTGAAATAATCGAGCGAGAAGCAATAATGCCGGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAAcc                                              <  1:1143438/147‑1 (MQ=255)
                                                                                                    gcgcCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCGGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCa                                             <  1:119216/149‑1 (MQ=255)
                                                                                                         cTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGGCATATTCTCCGCCACCAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCGGAGCCCACGTACCGATAGTTTCATAACCTGGGCGGCAGGCGATAAcc                                              >  2:657764/1‑143 (MQ=255)
                                                                                                                 gCAACTTCACTCATATAGGTGGCGCTGGTGGCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCGGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAaca                                >  1:1037822/1‑149 (MQ=255)
                                                                                                                 gCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCGGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAaca                                <  1:1039218/149‑1 (MQ=255)
                                                                                                                 gCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCGGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAaca                                <  1:580318/149‑1 (MQ=255)
                                                                                                                   aaCTTCCCTCGTATAGGTGGCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGGGCGAGAAGCAATAATGCCGGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAAcaca                              <  1:1103787/149‑1 (MQ=255)
                                                                                                                    aCTTCACTCATATAGGTGGCGCTGGTGCCATGTTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCGGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGaa                                   >  2:808527/1‑143 (MQ=255)
                                                                                                                     cTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCGGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATCCCCAGCGATCCGaaa                                  <  2:296602/143‑1 (MQ=255)
                                                                                                                     cTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCGGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGaaa                                  <  2:1060499/143‑1 (MQ=255)
                                                                                                                      ttCACTCATATAGGTGGCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAAAAAACGAGCGAGAAGCAATAATGCCGGAGCCCACGTACCTAAAGTTTCAAAACCTGGGAGGCAGGCGATAACCAGGGATCCCaacc                                 >  2:332736/1‑141 (MQ=255)
                                                                                                                        cactcctagcgcaggCGCTGGTGGCATATTCTCCGCCAACACATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCGGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAacac                               <  2:349019/130‑1 (MQ=255)
                                                                                                                         aCTCATATAGGTGGCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCGGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGCAAcaca                              >  2:927126/1‑143 (MQ=255)
                                                                                                                          cTCATATAGGTGGCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCGGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGGGATCCGAAACACATCATaca                       >  1:116131/1‑149 (MQ=255)
                                                                                                                          cTCATATAGGTGGCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCGGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACACATCATaca                       >  1:761134/1‑149 (MQ=255)
                                                                                                                              tataGGTGGCGCTGGTGCCATATTCTCCCCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCGGAGCCCACGTACCTATAGTTTCATACCCTGGGAGGCAGGCGATAACCAGCGATCCGAAACACATCATACACAcc                   <  1:472576/149‑1 (MQ=255)
                                                                                                                                    tGGCGCTGGTGGCATATTCTCCGCCAACAGATAAGCCCTGAAATAACCGAGCGAGAAGCAATAATGCCGGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACACATCATACACAcc                   <  2:325952/143‑1 (MQ=255)
                                                                                                                                     ggCGCTGGTGCCATATTCTCCGCCAACAGATACTCCCTGAAATAAACGAGCGAGAAGCAATAATGCCGGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACACATCATACACACCGATAACa            <  1:668318/149‑1 (MQ=255)
                                                                                                                                     ggCGCTGGGGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCGGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACACATCATACACACCGATAACa            <  1:559950/149‑1 (MQ=255)
                                                                                                                                      gcgcTGGTGGCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCGGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACACATCATACACCCCGATAACAg           >  1:142601/1‑149 (MQ=255)
                                                                                                                                      gcgcTGGTGCCATATTCTCCGCCAACAGATAATCACTGAAATAAACGAGCGAGAAGCAATAATGCCGGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACACATCATACACACCGa                 >  2:863788/1‑143 (MQ=255)
                                                                                                                                         ccggTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCGGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACACATCATACACACCGATAACAGCAt        <  1:1154737/147‑1 (MQ=255)
                                                                                                                                           ggTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCGGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACACATCTTACACACCGATAACAGCATCg      >  1:1007/1‑149 (MQ=255)
                                                                                                                                             tGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCGGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACACATCATACACACCGATAACAGCATCGAt    >  1:1095930/1‑149 (MQ=255)
                                                                                                                                               ccATATTCTCCGCCAAGAGCTAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCGGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACACATCATACACACCGATAACAGCAt        <  2:140523/143‑1 (MQ=255)
                                                                                                                                               ccATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCGGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACACATCATACACACCGATAACAGCATCGAttt  <  1:804613/149‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                                    
CAGCGTCTTCCATGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCCGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCGGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACACATCATACACACCGATAACAGCATCGATTT  >  CP009273/2718535‑2718826

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑