Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,298,277 T→C 13.2% intergenic (+405/+310) eco → / ← mqo ecotin, a serine protease inhibitor/malate dehydrogenase, FAD/NAD(P)‑binding domain

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,298,2770TC13.2% 240.5 / 9.5 152intergenic (+405/+310)eco/mqoecotin, a serine protease inhibitor/malate dehydrogenase, FAD/NAD(P)‑binding domain
Reads supporting (aligned to +/- strand):  ref base T (64/57);  new base C (11/13);  total (78/74)
Fisher's exact test for biased strand distribution p-value = 6.56e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 152 total aligned reads displayed.

GATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGC  >  CP009273/2298130‑2298421
                                                                                                                                                    |                                                                                                                                                
gATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGGGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTTGGTTGGATCGGGCGGTTACGCGGCATCCGACATCCAGCGTc                                                                                                                                                 >  1:1202426/1‑149 (MQ=11)
      gCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCATGCGCCGCCGTGAGGAGCCCAGCCCCCGGGGTTGGATAAGGCTGTTACGCCCCGTCCGGCACACAACGGCCGAGcc                                                                                                                                           >  1:322520/1‑149 (MQ=11)
       ccgtcaggccgtctCCGACATCTAGCGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGGCTTATCAGGCCTCCACCGCTGTGAAGTGCTCCTCCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTcc                                                                                                                                                <  2:669921/130‑1 (MQ=11)
        ggTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCgg                                                                                                                                         >  1:666904/1‑149 (MQ=38)
            aCGCCGCATCCGACATCTACCGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGc                                                                                                                                     <  1:408442/149‑1 (MQ=255)
            aCGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTAAACCGCTGTGAAGTGCTCCACCCCGTAGGTTAGTAAATTCGGTCACGCCGCATCCGACATCCAACGTCCGAGcc                                                                                                                                           <  2:194371/143‑1 (MQ=11)
              gccgcATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATACAACGTCCGAGCCgg                                                                                                                                         >  2:167598/1‑143 (MQ=38)
               ccgcATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTg                                                                                                                                  >  1:1196728/1‑149 (MQ=17)
                    tCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCACCGGCCGGGCCGGTCGccg                                                                                                                                   >  2:47180/1‑142 (MQ=255)
                           tCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAGGTGCTGCACCCCGTAGGGTGGGTAAGGCGGTTACGCCGCAGACGACAGCCAACGCCGGAGCCGGTTGCCCGATGCGa                                                                                                                            >  2:42983/1‑143 (MQ=11)
                            ccaaCGCCCAAGCCGGTGGCCTGAGGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGgc                                                                                                                     <  1:662564/147‑1 (MQ=255)
                             tAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGgcg                                                                                                                    >  1:261974/1‑149 (MQ=37)
                              aaCGCCCAAGCCGGTTGCTGGATGCGACGCTGGAGCGTCTTATCAGGCCTACACCGATGTGAGGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGTCGGTTGCCTGATGCGACCCCGgcgt                                                                                                                   >  1:954841/1‑148 (MQ=11)
                              aaCGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGgcgc                                                                                                                   <  1:60455/149‑1 (MQ=17)
                               aCGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCCGGCCTACACCGATGCGGAACGCTCCACCCCGTAGGTTGGATCAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGACGCGACGCTGGcgcg                                                                                                                  >  1:1155898/1‑149 (MQ=11)
                               aCGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCCGCcgcg                                                                                                                  >  1:1223447/1‑149 (MQ=255)
                               aCGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGAGGCGACGCGGCcgcg                                                                                                                  >  1:1223153/1‑149 (MQ=255)
                                cGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGt                                                                                                                 <  1:768156/149‑1 (MQ=39)
                                   ccAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCCCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACCTCCAACGTCCGACCCGTTTGACCGAAGCGACGCTGGCCCGCCtc                                                                                                              >  1:607711/1‑148 (MQ=11)
                                         cGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAgg                                                                                                        <  1:1149629/149‑1 (MQ=39)
                                         cGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAgg                                                                                                        <  1:1179734/149‑1 (MQ=39)
                                           tttGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGcc                                                                                                      <  1:1263340/148‑1 (MQ=40)
                                            ttGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCt                                                                                                     >  1:443539/1‑149 (MQ=39)
                                             ggCCTGATGCCGCGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGACACGGCGGTGACGCCGCACCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCa                                                                                                          <  2:428137/142‑1 (MQ=11)
                                              tcctcatcccaCGCTGCCGCCTCTCAACTGGCCTAAACCGCTGTGAAGTGCTGCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAg                                                                                                         <  2:1088942/133‑1 (MQ=11)
                                               cctcacgcgtcccTGGCGCGTCTTACCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAgg                                                                                                        <  2:920181/131‑1 (MQ=11)
                                               ccTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGAAGCGGGGCTGGCGCGTGTTACCCGGCCTacc                                                                                                  >  1:192735/1‑148 (MQ=14)
                                                 tGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACGTCCAAGGTCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGcc                                                                                                      >  2:871447/1‑143 (MQ=255)
                                                 tGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGCTACGCCGCATCCGACATCCAACGTCCGCGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTgcacg                                                                                                >  1:738081/1‑148 (MQ=255)
                                                    tGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCGCCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTGGCCTGATGCGCCGCTGGCGCGTCTTATCAGACCCACAccaat                                                                                             >  1:187406/1‑146 (MQ=14)
                                                    tGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACCCCGCt                                                                                             >  1:744754/1‑149 (MQ=25)
                                                     gCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGGCGCGGGAGCGCCTCGTGAGGCCTaca                                                                                                  >  2:727607/1‑143 (MQ=11)
                                                      cGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGGTACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGACATCGCAGGCCTaccc                                                                                                 >  2:1025367/1‑141 (MQ=11)
                                                        aCGCCGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATATGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGa                                                                                         <  1:158197/149‑1 (MQ=255)
                                                         cGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGaa                                                                                        <  1:995416/149‑1 (MQ=40)
                                                          gCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAg                                                                                       <  1:502770/149‑1 (MQ=25)
                                                            tGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGccatccccgcagt                                                                                           >  2:265131/1‑132 (MQ=255)
                                                             ggCGCGTCTTATCAGGCCTACCCCGCTGTGAAGTGCACCCCCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGGCCGAGCAGGTTGCCGGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGc                                                                                    <  1:255313/149‑1 (MQ=11)
                                                              gcgcGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGGCTAAAGCGACGCTGGCGCGTCTTATCCGGCCTACACCGCTGTGAAGTGCt                                                                                   >  1:1070692/1‑149 (MQ=255)
                                                              gcgcGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCATACACCGCTGTGa                                                                                         >  2:1044038/1‑143 (MQ=21)
                                                               cgcgTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTc                                                                                  <  1:1271265/149‑1 (MQ=255)
                                                                 cgTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGAAGCTGGCGCGTCTTATCAGCCCGACAccccgcccgcat                                                                                      >  2:222252/1‑133 (MQ=14)
                                                                    cTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGCGACGTGCTCCAccc                                                                             >  1:543140/1‑149 (MQ=255)
                                                                    cTTATCAGGCCTACACCGCTGTGAAGTGCCCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCAccc                                                                             <  1:28667/149‑1 (MQ=25)
                                                                         cAGGCCTACACCGCTGAGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGCGGCGTGCTCCACCCCGtgg                                                                        >  1:814299/1‑147 (MQ=255)
                                                                          cggcacacacCGCTGTGCAGCGAGCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTAGCGCGGCACATCAGCCCGTCGCCGCAGGGAGGTGGTCCAcct                                                                             >  2:521542/7‑142 (MQ=2)
                                                                          aGGCCGACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCAGATCGGACGCGCGCCCGGCTTGTAAGGCGTGCTGCGCTCTGAGGTGATcccccc                                                                             >  2:795231/1‑143 (MQ=2)
                                                                             cccacacCGCTGGCAAGTGCGCCACCCCGTAGGTCGTATAAGGCGGGTACGCCTCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCGGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCg                                                                    <  1:1085988/146‑1 (MQ=11)
                                                                              cTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCgg                                                                   <  1:689914/149‑1 (MQ=255)
                                                                              cTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCgg                                                                   <  1:507089/149‑1 (MQ=17)
                                                                               tACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAAGGACCGAGCCGGTTGCCTGATGCGACGTTGGCCCGTCTTATCAGGCCGACGCGGCTGTGACGTGccacaacgcggag                                                                        >  2:76619/1‑131 (MQ=11)
                                                                               tACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTCCACCCCTGTGGCGTGCTCCAcccccgag                                                                        >  2:1067033/1‑139 (MQ=14)
                                                                                acacCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACCCCGCTGCGGCGTGCCCCGCCCGGTggg                                                                       >  2:944243/1‑143 (MQ=14)
                                                                                  acCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGGCGCGGGAGCGTCCTCGCCGGCCCACCCCTCTGCGGAGTGCTCCACCCCGTGGGTCGGCTac                                                               >  1:387220/1‑148 (MQ=14)
                                                                                    cGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAgg                                                             >  1:814437/1‑149 (MQ=255)
                                                                                    cGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAgg                                                             <  1:344110/149‑1 (MQ=255)
                                                                                    cGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCCCTACACCCCTGTGACGGGCTCCACCCCGTCGGACGGCTAggg                                                             >  1:543937/1‑149 (MQ=14)
                                                                                    cGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGCCCAAGCCGGTTGCCTGCTGCGACGCTGGCGCGTCGTATCAGGCCTCCCCCGCGGGGGCGTGCTCCACCGCGTAGGTCgg                                                                   >  2:1135215/1‑143 (MQ=11)
                                                                                     gCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGGAGTGCTCCACCCCGTAGGCCGGATAAGGc                                                            >  1:833816/1‑149 (MQ=255)
                                                                                      ctggcaaGTGCTCCACCCCGCAGGTTGGATAAGGCGGGCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCg                                                           <  1:287221/144‑1 (MQ=11)
                                                                                      cggtgAAGTGCTCCCCCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCg                                                           <  1:804279/147‑1 (MQ=255)
                                                                                      cggtgAAGTGCCCCGCCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCg                                                           <  1:423811/147‑1 (MQ=255)
                                                                                      cTGTGAAGTGCTCCCCCCCGTAGGTTGGATAAGGCGGTCACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCg                                                           <  1:977516/149‑1 (MQ=255)
                                                                                      cTGTGAAGTGCTCCACCCCGTAGGTTGGAGAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCg                                                           <  1:190761/149‑1 (MQ=255)
                                                                                      cTGTGAAGTGCTCCACCCCGGAGGTTGGATCAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCg                                                           <  1:142969/149‑1 (MQ=255)
                                                                                          gAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTa                                                       <  1:807229/149‑1 (MQ=255)
                                                                                          gAAGTGCACCACCCCGTAGGCTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTa                                                       <  1:702663/149‑1 (MQ=255)
                                                                                           aaGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCCCTCCACCCCTGTGACGTGCTCCACCCCGTAGGGCGGATAAGGCGGttcc                                                      >  1:442077/1‑147 (MQ=14)
                                                                                            attGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTAcg                                                     <  1:613927/147‑1 (MQ=255)
                                                                                            aGTGCTCCACCCCGTAGGTCTGATAAGGCGGTTACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTCTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCg                                                           <  2:1118181/143‑1 (MQ=255)
                                                                                            aGTGCGCCACCCCGTAGGTTGTATACGGCGGTTACCCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCg                                                           <  2:167724/143‑1 (MQ=255)
                                                                                              tGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTAcgcc                                                   >  1:1300853/1‑149 (MQ=255)
                                                                                                 tCCACCCCGTAGGTTGGATAAGTCGGTTACGCCAACCTCTGCATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGACTACCCCGCTGCGAAGTGCTCCACCCCGTAGGGCGGATAAGGAGGTTACGTCGCa                                                >  1:314282/1‑149 (MQ=14)
                                                                                                    cccccGTAGGTGGGTTATGGCGGTTACGCCGCGTCCGCCATCGAGCGTCCGAGGCGGTTGCCTGATGCGACGCTGGCGGGTGTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTAcgcc                                                   <  2:723667/142‑1 (MQ=11)
                                                                                                      cccGTCGGTTGGATACGGCGGTTACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTAcgccgc                                                 <  2:692571/143‑1 (MQ=255)
                                                                                                      cccGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTATGTCGGATAAGGCGGTTAcgccgc                                                 <  2:193221/143‑1 (MQ=255)
                                                                                                         gTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAAAGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTGCGCCGCACCCGAcct                                        >  1:380896/1‑147 (MQ=255)
                                                                                                          tAGGTTCGATAAGGCGGTTACGCCGCATCCGACATCCAACGCCCGCGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATc                                       <  1:105432/149‑1 (MQ=255)
                                                                                                            ggTCGGATCAGGCCGTTCCTCCGCACCCGACATCCACCGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGa                                           <  2:624594/143‑1 (MQ=11)
                                                                                                                ggATAAGGCGGTTACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGc                                 <  1:556150/149‑1 (MQ=17)
                                                                                                                agATAAGGCGGTTACGCCGCATCCCACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGc                                 <  1:1007687/148‑1 (MQ=25)
                                                                                                                 gATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTCCCCCGCTGTGAAGTGCCCCACCCCGTAGGGCGGATAAGGCGGGTCCGCCGCCTCCGCCacgc                                      >  2:646300/1‑140 (MQ=11)
                                                                                                                    aaGGCGTTTACGCCGCATCCGACATCCAACGTCCGGGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCaa                             <  1:922394/149‑1 (MQ=21)
                                                                                                                    aaGGCGGTTACGCCGCATCCGACGTCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCaa                             >  1:852008/1‑149 (MQ=25)
                                                                                                                    aaGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCAACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGAGGTTACGGCGCATCCGACATCTAACGCCCag                             >  1:265675/1‑148 (MQ=255)
                                                                                                                       gCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACgag                                >  2:409321/1‑141 (MQ=25)
                                                                                                                       gCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCCCTACCCCGCTGCGACGTGCTCCACCCCGTAGGGCGGATAAGCCGGTTACGCCGCATCCGACACCCAACGcc                                >  2:813938/1‑143 (MQ=11)
                                                                                                                        cGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCg                         <  1:1132775/149‑1 (MQ=34)
                                                                                                                           ttACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACCCCGCATCCGCCATCTAACGCCCCAGCCGGtt                      >  1:150380/1‑149 (MQ=16)
                                                                                                                             ccgccgcATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGTTGGTGCTTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGc                    <  1:363711/148‑1 (MQ=12)
                                                                                                                             aCGCCGCATCCGACATCCCACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGc                    <  1:912485/149‑1 (MQ=17)
                                                                                                                              cgccgcATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCGTCCGACATCTAACGCCCAAGCCGGTTGcc                   >  1:1035222/1‑149 (MQ=17)
                                                                                                                               gccgcATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATCAGGCGGTTACGCCGCATCCGCCATCTAACGCCCCAGCCgg                        >  2:527759/1‑143 (MQ=12)
                                                                                                                                 ccgcgcccGCCATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGt                       <  2:1081736/137‑1 (MQ=12)
                                                                                                                                          aCATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTCCACCGCTGTGAAGTGCTCCGCCCCGTAGGGCGGATAAGGCGGTTACGCCGCATCCGACGTCTACCGCCCAAGCCGGTTGCCTGATg              >  2:1028433/1‑143 (MQ=11)
                                                                                                                                             tCCACCGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCACATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTgg     <  1:1089262/149‑1 (MQ=17)
                                                                                                                                             tCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTgg     <  1:996431/149‑1 (MQ=32)
                                                                                                                                              ccAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGAc          >  2:161446/1‑143 (MQ=32)
                                                                                                                                              ccAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCCCTCCGAAGTGCCCCGCCCCGTAGGTCGGATAAGGCGGTTCCACCGCATCCGACGACCAACGCCCAAGCCGGGTGCCTggggccac          >  2:771705/1‑136 (MQ=11)
                                                                                                                                                aaCGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGAGGCTGgcgc  >  1:206369/1‑149 (MQ=17)
                                                                                                                                                    |                                                                                                                                                
GATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGC  >  CP009273/2298130‑2298421

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: