Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,191,760 G→A 13.4% E394E (GAG→GAA icd → e14 prophage; isocitrate dehydrogenase, specific for NADP+

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,191,7600GA13.4% 187.7 / 13.5 76E394E (GAG→GAAicde14 prophage; isocitrate dehydrogenase, specific for NADP+
Reads supporting (aligned to +/- strand):  ref base G (38/27);  new base A (7/3);  total (46/30)
Fisher's exact test for biased strand distribution p-value = 7.31e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.20e-01

CCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACGGGAGCGTAACGCTCCCGTTGTTTTTTGTTAGGCTGCTAACGGTTATCAAAA  >  CP009273/1191615‑1191907
                                                                                                                                                 |                                                                                                                                                   
ccGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGt                                                                                                                                                  >  1:1120615/1‑149 (MQ=14)
 cGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGAACTGAAGCGGCTGACCTGATTGTTAAAGGTGTGGAAGGCGCAATCAATGCCAAGACCGTAACTTGTGACTTCGAACgac                                                                                                                                                 >  1:119041/1‑147 (MQ=14)
    tCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGTTGCTGCGCCGCATGGGTTGGACGGAAGCGGGTGAGTTGATTGTTAAAGGTATGGAAGGCGCAATCAAAGCGAAAACCGTAGCGTCTGATTTTGAGc                                                                                                                                                    >  2:322706/1‑143 (MQ=21)
    tCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTga                                                                                                                                              >  1:1075035/1‑149 (MQ=255)
     cAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTgat                                                                                                                                             <  1:323611/149‑1 (MQ=255)
       ggACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTc                                                                                                                                                 >  2:344124/1‑143 (MQ=255)
       ggACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAACGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTc                                                                                                                                                 >  2:549664/1‑143 (MQ=255)
       ggACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGATTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTc                                                                                                                                                 >  2:840939/1‑143 (MQ=255)
        gACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTCGACCGAAGCGGCTGAGTGAATGGTTAAAGGTATGGAAGGCGCAAACGACGCGAAAAGCGTAACCTATGACTTCGAGCGTCt                                                                                                                                                >  2:604/1‑143 (MQ=37)
        gACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATAGGGTGGTCCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCCATCAACGCGAAACCCGTAACCTATGACTTCGAGCGTCTgatgga                                                                                                                                          >  1:1083712/1‑149 (MQ=37)
            aaGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAATACCGTAACCTATGACTTCGAGCGTCTgatg                                                                                                                                            >  2:174325/1‑143 (MQ=255)
             aGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTCTGATGGAAGgcg                                                                                                                                     <  1:1265474/149‑1 (MQ=11)
                aaaTCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTCTGATGGAAGGCGCTa                                                                                                                                  >  1:269484/1‑149 (MQ=11)
                aaaTCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAACGGCTAACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGATTTCGAACGTCTGATGGAAGGCGCTa                                                                                                                                  >  1:797059/1‑149 (MQ=11)
                 aaTCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATGTGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTCTGatggaagg                                                                                                                                       <  2:764645/143‑1 (MQ=1)
                      tGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATAAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAActgc                                                                                                                            <  1:1183783/149‑1 (MQ=255)
                        gCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATCTGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTCTGATGGAAGGCGCTAAGctgctg                                                                                                                          >  1:1148655/1‑149 (MQ=14)
                         ctctATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAAc                                                                                                                               <  2:897479/143‑1 (MQ=255)
                          tctATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGACCGTCTGTTGGAAGGCGCTAAGct                                                                                                                              >  2:570638/1‑143 (MQ=11)
                          tctATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTCTGATGGAAGGCGCTAAGct                                                                                                                              >  2:514624/1‑143 (MQ=14)
                          gctATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTTAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTCTGATGGAAGGCGCTAAGCTGCTGaa                                                                                                                        >  1:663760/2‑149 (MQ=14)
                            tattatTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAAt                                                                                                                      >  1:124315/1‑149 (MQ=255)
                            tattatTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAAt                                                                                                                      >  1:1147761/1‑149 (MQ=255)
                               tattCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGCCTTCGAGCGTCTGATGGAAGGCGCTAAACTGCTGa                                                                                                                         >  2:474746/1‑143 (MQ=255)
                                    tctcCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCagag                                                                                                              >  1:764259/1‑149 (MQ=255)
                                        cGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTg                                                                                                          <  1:1214061/149‑1 (MQ=255)
                                         gCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTgg                                                                                                         <  1:743923/149‑1 (MQ=255)
                                         gCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGGATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGCCTTCGAGCGTCTGATGGATGGCGCTAACCTGCTGAACTGTTCaga                                                                                                               >  2:370352/1‑143 (MQ=255)
                                            gagaTGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGttacgaggt                                                                                                            >  2:100895/1‑136 (MQ=255)
                                            gagaTGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGa                                                                                                      >  1:1264393/1‑149 (MQ=255)
                                                  agtctgcCCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGa                                                                                                      <  2:789271/140‑1 (MQ=255)
                                                    gctgcGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGatca                                                                                              <  1:282381/149‑1 (MQ=255)
                                                     ctgcGCCACTGGGGTTGGACCGAAGTGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGAcgc                                                                                                   <  2:145973/143‑1 (MQ=255)
                                                       gcgcCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCg                                                                                           >  1:1029371/1‑149 (MQ=255)
                                                         gcCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGTAAGGCGCGATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGaa                                                                                         <  1:961858/149‑1 (MQ=255)
                                                         gcCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGaa                                                                                         <  1:238035/149‑1 (MQ=255)
                                                            acaTGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGCTTGGTGACGCGctcctc                                                                                            >  2:255908/1‑143 (MQ=255)
                                                            acaAGGGTTGGACCGAAGCGGCTTACTTGTTTTTTAAAGGTATTGAAGGAGCAATCAACGCGAAAACCGTAGCCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAAc                                                                                      <  1:233749/149‑1 (MQ=17)
                                                                  gTTGCACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAAc                                                                                      <  2:1082010/143‑1 (MQ=255)
                                                                   ttGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACa                                                                                     >  2:1039046/1‑143 (MQ=255)
                                                                           aaGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGCACTGTTCAGCGTTTGGTGACGCGATCATCGAAAACATGTAATGCcgtcgt                                                                       >  1:1056126/1‑149 (MQ=255)
                                                                             gCGGTTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGttt                                                                     <  1:1109624/149‑1 (MQ=255)
                                                                               ggCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGGGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGt                                                                   <  1:599544/149‑1 (MQ=255)
                                                                                    aCTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAAt                                                              <  1:1036943/149‑1 (MQ=255)
                                                                                        aaTTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTaa                                                                >  2:821804/1‑143 (MQ=255)
                                                                                        aaTTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTaa                                                                >  2:622595/1‑143 (MQ=255)
                                                                                        aaTTGTTAAAGGATTGGAAGGCGCAACCAACGCGAACACCGTAACCTATGACTTCGAGCGTCTGATGGCTGGCGCCAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTaa                                                                <  2:713225/143‑1 (MQ=18)
                                                                                         aTTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGCTCATCGAAAACATGTAATGCCGTAGTTTGTTaaa                                                               >  2:525005/1‑143 (MQ=255)
                                                                                                   gTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATCTATTAACGGGAGCg                                               >  1:281813/1‑149 (MQ=255)
                                                                                                       ggAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACGGGAg                                                 >  2:532243/1‑143 (MQ=255)
                                                                                                        gAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACGGGAGCGTAACg                                          >  1:320434/1‑149 (MQ=255)
                                                                                                            gcgcAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTGTGTTAAATTTATTAACGGGAGCGTAGCGCTcc                                      >  1:349599/1‑149 (MQ=255)
                                                                                                             cgcAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACGGGAGCGTCACGCTccc                                     >  1:1075172/1‑149 (MQ=255)
                                                                                                              gcAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACGGGAGCGTAACGCTCCCg                                    >  1:221225/1‑149 (MQ=255)
                                                                                                                        cgAAAACCGTAACCTAGGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACGGGAGCGTTACGCTCCCgttaataaatt                          <  1:207966/149‑9 (MQ=25)
                                                                                                                          aaaaCCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACGGGAGCATAACGCTCCCGTTGTTTTTTGtt                        <  1:851733/149‑1 (MQ=255)
                                                                                                                          aaaaCCGTAACCTAGGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACGGGAGCGTAACGCTCCCGTTGTTTTTTGtt                        <  1:409463/149‑1 (MQ=255)
                                                                                                                             tccGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTCCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACGGGAGCGTAACGCTCCCGTTGtttttt                           <  2:131806/142‑1 (MQ=255)
                                                                                                                             aCCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACGGGAGCGTAACGCTCCCGTTGtttttt                           <  2:1088667/143‑1 (MQ=255)
                                                                                                                              ccGTAACCTATGAGTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAGATTTATTAACGCGAGCGTAACGCTCCCGTTGTTTTTTGTTAGgc                    >  1:885908/1‑149 (MQ=255)
                                                                                                                               cggagccTATGAGTTGGAGCGTCTGAGGGATGGCGCTATACTGCTGAAATGTTCGGAGTTTGGGGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACGGGAGCGTAACGCTCCCGTTGTTTTTTGt                         <  2:163457/138‑1 (MQ=37)
                                                                                                                               cGTAACCTATGACTTCGAACGTCTGATGGATGGCGCTAAACTGCTCAAATGTTCAGAGTTTGGTTACGCTATCATCGAAAACATGTAATGCCTTAGTTTTTTAAATTTATTAACTGGCGCGTAACGCTCCCGCTGTTTTTTGt                         <  2:361567/143‑1 (MQ=21)
                                                                                                                                   aCCTATGACTTCGGGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACGGGAGCGTAACGCTCCCGTTTTTTTGTGGTAgg                     <  2:694102/143‑1 (MQ=255)
                                                                                                                                     cTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTACCGGGAGCGTAACGCTCCCGTTGTTTTTTGTTAGgct                   >  2:525264/1‑143 (MQ=255)
                                                                                                                                      tATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACGGGAGCGTAACGCTCCCGTTGTTTTTTGTTAGGCTGCTAACg            <  1:1045525/149‑1 (MQ=255)
                                                                                                                                      tATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAATTTGTTAAATTTATTAACGGGTGCGTAACGCTCCCGTTGTTTTTTGTTAGgctg                  >  2:135917/1‑143 (MQ=255)
                                                                                                                                       aTGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACGGGAGGGTAACGCTCCCGTTGTTTTTTGTTAGgctgc                 >  2:334955/1‑143 (MQ=255)
                                                                                                                                        tGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACGGGGGCGTAACGCTCCCGTTGTTTTTTGTTAGGCTGCTAACGGt          >  1:34077/1‑149 (MQ=255)
                                                                                                                                        tGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACGGGAGCGTAACGCTCCCGTTGTTTTTTGTTAGgctgct                >  2:833309/1‑143 (MQ=255)
                                                                                                                                         gACTTCGAGCGGCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACGGGAGCGTAACGCTCCCGTTGTTTTTTGTTAGGCTGCTa               >  2:545025/1‑143 (MQ=255)
                                                                                                                                          gcTTCGAGCGTCTGGTGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACGGGAGCGTAACGCTCCCGTTGTTTTTTGTTAGGCTGCTAACGGTTa        <  1:911857/148‑1 (MQ=255)
                                                                                                                                          aCTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACGGGAGCGTAACGCTCCCGTTGTTTTTTGTTAGGCTGCTAACGGTTa        <  1:1027694/149‑1 (MQ=255)
                                                                                                                                          aCTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACGGGAGCGTAACGCTCCCGTTGTTTTTTGTTAGGCTGCTAACGGTTa        <  1:501661/149‑1 (MQ=255)
                                                                                                                                            ttCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACGGGAGCGTAACGCTCCCGTTGTTTTTTGTTAGGCTGCTAACGGTTATc      >  1:439801/1‑149 (MQ=255)
                                                                                                                                              cGAGCGTCTGATGGATGGCGCTAAAATGCTGAACCCTTCAACGTGTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACGGGAGCGTAACGCTCCCGTTGTTTTTTGTTAGGCTGCTAACGGt          >  2:193588/1‑143 (MQ=38)
                                                                                                                                                aGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACGGGGGCGTAACGCTCCCGTTGTTTTTTGTTAGGCTGCTAACGGTTATCaaaa  >  1:581370/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                                   
CCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACGGGAGCGTAACGCTCCCGTTGTTTTTTGTTAGGCTGCTAACGGTTATCAAAA  >  CP009273/1191615‑1191907

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: