Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,067,611 G→T 5.7% P2Q (CCA→CAA)  rutC ← putative aminoacrylate deaminase, reactive intermediate detoxification; weak enamine/imine deaminase activity

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,067,6110GT 5.7% 214.0 / 3.0 71P2Q (CCA→CAA) rutCputative aminoacrylate deaminase, reactive intermediate detoxification; weak enamine/imine deaminase activity
Reads supporting (aligned to +/- strand):  ref base G (40/26);  new base T (2/2);  total (43/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.95e-01

GCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAGCGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTGGCATCGTTAAACTCCTTAAGCGATATGAGCAAAGGACGTGGGAGAAAGCGCGTCGCAG‑‑AATGTTTCGACGTCGCTGACCCAGCCAAAAAAGGTTTCGATATTGAACAACGCGGCTTTCTGCGCAAATTTCGGCCCCGCCTGGTGAGT  >  CP009273/1067467‑1067758
                                                                                                                                                |                                                                                                                                                     
gcCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAGCGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCGCTGCTGCCGGCGGGAATAATTACGGGTTTTGGCAt                                                                                                                                                   >  1:1129215/1‑149 (MQ=255)
gcCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAGCGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTGGCAt                                                                                                                                                   <  1:1073115/149‑1 (MQ=255)
gcCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAGCGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTGGCAt                                                                                                                                                   <  1:1016310/149‑1 (MQ=255)
 ccTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAGCGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGCTTTTGGCATc                                                                                                                                                  >  1:177656/1‑149 (MQ=255)
  cTTTGGGTCATCGGCAAACAGCACGTTATGATGTTGATCAAAAGCCAGCGTACCGGAGACATACACCACGCCATCAGCCAGCGTGTCGGGAACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTGGCACCg                                                                                                                                                 >  1:28879/1‑149 (MQ=255)
    ttgggcactCGGCCAACAGCACGTTATTATGTTGAGCAAAAGCCAGCGTACCGGAGACAGACACCACGCCATCAGCCAGCGTGCCGGGAACGAAGCGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTGGCATCGtt                                                                                                                                               <  1:741723/141‑1 (MQ=255)
    ttGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAGCGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTGGc                                                                                                                                                     <  2:20144/143‑1 (MQ=255)
    ttGGGTCATCGGCAAACAGCACGTTATTATATTGATCAAAAGCCAGCGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTGGc                                                                                                                                                     <  2:324553/143‑1 (MQ=255)
       ggTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAGCGTACCGGAGACATACACCACGCCATCAGACAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTGGCATCGTTaaa                                                                                                                                            <  1:96451/149‑1 (MQ=255)
         tCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAGCGTACCGGAGACATACACCACGCCAGCAGCCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTGGCATCGTTAAACt                                                                                                                                          >  1:891176/1‑149 (MQ=255)
            tCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAGCGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTGGCATCGTTAAACTCCt                                                                                                                                       >  1:251543/1‑149 (MQ=255)
             cGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAGCGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACCGATTTTGGCCTCGTTAAACTCCtt                                                                                                                                      >  1:31811/1‑149 (MQ=255)
                 aaaCAGCACGTTATTATGTTGATCAAAAGCCAGCGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGGACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAGTTACGGATTTTGGCATCGTTAAACTcc                                                                                                                                        >  2:27727/1‑143 (MQ=255)
                 aaaCAGCACGTTATTATGTTGATCAAAAGCCAGCGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGACTTTGGCATCGTTAAACTcc                                                                                                                                        >  2:283999/1‑143 (MQ=255)
                    cAGCACGTTATTATGTTGATCAAAAGCCAGCGGACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCCGGAATAATTACGGATTTTGGCATCGTTAAACTCCTTAAGCGat                                                                                                                               <  1:1251247/149‑1 (MQ=255)
                       cACGTTATTATGTTGATCAAAAGCCAGCGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAAGTACGGGTTTTGGCATCGTTCAACTCCTTAAGCGagttg                                                                                                                            >  1:735378/1‑145 (MQ=255)
                           ttattaTGTTGATCAAAAGCCAGCGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTGGCATCGTTAAACTCCTTAAGCGaaa                                                                                                                              >  2:288083/1‑141 (MQ=255)
                              ttatGTTGATCAAAAGCCAGCGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCGGGGATAATTACGGATTTGGGCATCGTTAAACTCCTTAAGCGATATGa                                                                                                                           >  2:38694/1‑143 (MQ=255)
                                 tGTTGATCAAAAGCCAGCGTACCGGAGAAATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTCGCATCGTTAAACTCCTTAAGCGATATGAGCAAAGGAc                                                                                                                  >  1:849676/1‑149 (MQ=255)
                                      aTCAAAAGCCAGCGTACCGGAGACATAAAACAAGAGGTCATCCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTGGCATCGTTAAACTCCTTAAGCGATATGAGCAAAGGa                                                                                                                   >  2:516362/1‑143 (MQ=255)
                                          aaaGCCAGCGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCGCTGCTGCCACCGGGAATAATTACGGATTTTGGCATCGTTAAACTCCTTAAGCGATATGAGCAAAGGACGTGGGAGaa                                                                                                         <  1:786206/149‑1 (MQ=255)
                                               cAGCGTACCGGAGACATACACCCCGCCATCAGCCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTGGCATCGTTAAACTCCTTAAGCGATATGAGCAAAGGACGTGGGAGAAAgcgc                                                                                                    <  1:1019462/149‑1 (MQ=255)
                                                 gCGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTGCCATCGTTAAACTCCTTAAGCGATATTATCAAAGGACGTTGGAGaaa                                                                                                        <  2:102539/143‑1 (MQ=255)
                                                   gTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAGGGGGCCAGCGGCGGTGAGCGTCCAGCGGGAATAATTACGGATTTTGGCATCGTTAAACTCCTTAAGCGATATGAGCAAAGGACGTGGGAGAAAGCGCGTCg                                                                                                <  1:1275862/149‑1 (MQ=255)
                                                    tACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTGGCATCGTTAAACTCCTTAAGCGATATGAGCAAAGGACGTGGGAGAAAgcg                                                                                                     <  2:364219/143‑1 (MQ=255)
                                                     aCCGGAGACAAACACCACGCCATCAGCCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTGGCATCGTTAAACTCCTTAAGCGATATGAGCAAAGGACGTGGGAGAAAgcgc                                                                                                    <  2:779103/143‑1 (MQ=255)
                                                              aTACACCACGCCATCAGCCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTGGCATCGTTAAACTCCTTAAGCGATATGAGCAAAGGACGTGGGAGAAAGCGCGTCGCAG‑‑AATGTTTc                                                                                   >  1:759396/1‑149 (MQ=255)
                                                              aTACACCACGCCATCAGCCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTGGCATCGTTAAACGCCTTAAGCGATATGAGCAAAGGACGTGGGAGAAAGCCCGTCGCAG‑‑aa                                                                                         >  2:376739/1‑143 (MQ=255)
                                                               tACACCACGCCATCAGCCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTGGCATCGTTAAACTCCTTAAGCGGTATGAGCAAAGGACGTGGGAGAAAGCGCGTCGCAG‑‑AAt                                                                                        >  2:912367/1‑143 (MQ=255)
                                                               tACACCACGCCATCAGCCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTGGCATCGTTAAACTCCTTAAGCGATATGAGCAAAGGACGTGGGAGAAAGCGCGTCGCCG‑‑AATGTTTCg                                                                                  >  1:140109/1‑149 (MQ=255)
                                                               tACACCACGCCATCAGCCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCGAGAATAATTACGGATTTTGGCATCGTTAAACTCCTTAAGCGATATGAGCAAAGGACGTGGGAGAAAGCGCGTCGCAG‑‑AATGTTTCg                                                                                  >  1:870498/1‑149 (MQ=255)
                                                                    cacGCCATCAGCCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTGGCATCGTTAAACTCCTTAAGCGATATGAGCAAAGGGCGTGGGAGAAAGCGCGTCGCAG‑‑AATGTTTCGCCGTc                                                                             >  1:1029190/1‑149 (MQ=255)
                                                                       gCCATCAGCCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAAGAATTACGGATTTTGGCATCGTTAAACTCCTTAAGCGATATGAGCAAAGGACGTGGGAGAAAGCGCGTCGCAG‑‑AATGTTTCGACGTCGCt                                                                          <  1:467791/149‑1 (MQ=255)
                                                                               ccagcGTGCCGGGAGCGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTGGCATCGTTAAACTCCTTAAGCGATATGAGCAAAGGACGTGGGAGAAAGCGCGTCGCAG‑‑AATGTTTCGACGTCGCTGACCCAGc                                                                  <  1:8585/149‑1 (MQ=255)
                                                                               ccagcGTGCCGGGAATGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTGGCATCGTTAAACTCCTTAAGCGATATGAGCAAAGGACGTGGGAGAAAGCGCGTCGCAG‑‑AATGTTTCGACGTCGCTGa                                                                        >  2:1128690/1‑143 (MQ=255)
                                                                               ccagcGTGCCGGGAACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCGGGCATAATTACGGATTTTGGCATCGTTAAACTCCTTAAGCGATATGAGCAAAGGACGTGGGAGGAAGCGCGTCGGAA‑‑AATGTTTCGACGTCGCTTACCCAGc                                                                  >  1:425279/1‑149 (MQ=255)
                                                                                     tGCCGGGAACGAGGGGGGTCAGCGGTGCGCTGCTGCCAGCGGGAATAATTATGGATTTTGGCATCGTTAAACTCCTTAAGCGATATGAGCACAGGACGTGGGAGAAAGCGCGTCGCAG‑‑AATGTTTCGACGTCGCTGACCCaac                                                                  <  2:1058038/143‑3 (MQ=255)
                                                                                        cGGGAACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTGGCATCGTTAAACTCCTTAAGCGATATGAGCAAAGGACGTGGGAGAAAGCGCGTCGCAG‑‑AATGTTTCGACGTCGCTGACCCAGCCaa                                                               >  2:1105531/1‑143 (MQ=255)
                                                                                          ggAACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTGGCATCGTTGAACTCCTTAAGCGGTATGAGCAAAGGACGTGGGAGAAAGCGCGTCGCAG‑‑AATGTTTCGACGTCGCTGACCCAGCCAAAAAAGGtt                                                       >  1:1025444/1‑149 (MQ=255)
                                                                                          ggAACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTGGCATCGTTAAACTCCTTAAGCGATATGAGCAAAGGACGTGGGAGAAAGCGCGTCGCAG‑‑AATGTTTCGACGTCGCTGACCCAGCCAAAAAAGGtt                                                       >  1:1025729/1‑149 (MQ=255)
                                                                                           gAACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTGGCATCGTTAAACTCCTTAAGCGATATGAGCAAAGGACGTGGGAGAAAGCGCGTCGCAGTTATTGTTTGCGTCGCTGACCCAGCCAAAAAAGGttt                                                      <  1:1287222/149‑1 (MQ=255)
                                                                                             aCGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATACTTACGGATTTTTGCATCGTTAAACTCCTTAAGCGATATGAGCAAAGGACGTGGGAGAAAGTGCGTCGCAG‑‑AATGTTTCGACGTCGCTGACCCAGCCAAAAAAg                                                          >  2:686292/1‑143 (MQ=255)
                                                                                             aCGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTTGCATCGTTAAACTCCTTAAGCGATATGAGCAAAGGACGTGGGAGAAAGCGCGTCGCAG‑‑AATGTTTCGACGTCGCTGACCCAGCCAAAAAAg                                                          >  2:127231/1‑143 (MQ=255)
                                                                                              cGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTGGCATCGTTAAACTCCTTAAGCGATATGAGCAAAGGACGTGGGAGAAAGCGCGTCGCAG‑‑AATGTTTCGACGTCGCTGACCCAGCCAAAAAAGGTTTCGa                                                   <  1:41791/149‑1 (MQ=255)
                                                                                               gAAGGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTGGCATCGTTAAACTCCTTAAGCGATATGAGCAAAGGACGTGGGAGAAAGCGCGTCGCAG‑‑AATGTTTCGACGTCGCTGACCCAGCCAAAAAAGGTTTCGat                                                  >  1:39319/1‑149 (MQ=255)
                                                                                               gAAGGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTGGCATCGTTAAACTCCTTAAGCGATATGAGCAAAGGACGTGGGAGAAAGCGCGTCGCAA‑‑AATGTTTCGACGTCGCTGACCCAGCCAAAAAAGGt                                                        >  2:421880/1‑143 (MQ=255)
                                                                                                aaGGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTGGCATCGTTAAACTCCTTAAGCGATATGAGCAAAGGACGTGGGAGAAAGCGCGTCGCAG‑‑AATGTTTCGACGTCGCTGACCCAGCCAAAAAAGGtt                                                       >  2:1010448/1‑143 (MQ=255)
                                                                                                aaGGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTGGCATCGTTAAACTCCTTAAGCGATATGAGCAAAGGACGTGGGAGAAAGCGCGTCGCAG‑‑AATGTTTCGACGTCGCTGACCCAGCCAAAAAAGGtt                                                       >  2:918365/1‑143 (MQ=255)
                                                                                                aaGGGGGCAAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTGGCATCGTTAAACTCATTGAGCGATATGAGCAAAGGACGTGGGGGAAAGCGCGTGGCAG‑‑AATGGTTCGACATCGCTGACCCAGCCAAAAAAgggt                                                       >  2:1070839/1‑141 (MQ=255)
                                                                                                   ggggCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTGGGCATCGTTAAACTACTTAAGCGATATGAGCAAAGGACGTGGGAGAAAGCGCGTCGCAG‑‑AATGTTTCGACGTCGCTGACCCAGCCAAAAAAGGTTTCGATATTg                                              <  1:533580/149‑1 (MQ=255)
                                                                                                      gCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTGGCATCGTTAAACTCCTTAAGCGATATGAGCAAAGGACGTGGGAGAAAGCGCGTCGCAG‑‑AATGTTTCGACGTCGCTGACCCAGCCAAAAAAGGTTTCGata                                                 >  2:475257/1‑143 (MQ=255)
                                                                                                        cAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTGGCATCGTTAAACTCCTTAAGCGATATGAGCAAAGGACGTGGGAGAAAGCGCGTCGCAG‑‑AATGTTTCGCCGTCGCTGACCCAGCCAAAAAAGGTTTCGATAtt                                               >  2:179260/1‑143 (MQ=255)
                                                                                                        cAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTGGCATCGTTAAACTCCTTAAGCGATATGAGCAAAGGACGTGGGAGAAAGCGCGTCGCAG‑‑AATGTTTCGACGTCGCTGACCCAGCCAAAAAAGGTTTCGATGtt                                               >  2:218668/1‑143 (MQ=255)
                                                                                                        cAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTGGCATCGTTAAACTCCTTAAGCGATATGAGCAAAGGACGTGGGAGAAAGCGCGTCGCAG‑‑AATGTTTCGACGTCGCTGACCCAGCCAAAAAAGGTTTCGATAtt                                               >  2:912457/1‑143 (MQ=255)
                                                                                                          gCGGGGCGCTGCTGCCAGCGGGAATAATTACGGATTTTGGCATCGTTAAACTCCTTAAGCGATATGAGCAAAGGACCTGGGAGAAAGCGCGTCGCAG‑‑AATGTTTCGACGTCACTGCCCCAGCCAAAAAAGGTTTCGATATTGAACAAcg                                       <  1:701569/149‑1 (MQ=255)
                                                                                                                cgcTGCTGCCAGCGGGAATAATTACGGATTTTGGCATCGGTAAACTCCGTAAGCGATATGAGCAAAGGACGTGGGAGAAAGCGCGTCGCAG‑‑AATGTTTCGACGTCGCAAACACAAAAAAAAAAGGTTTCGATATTGAACAAcg                                       <  2:901989/143‑1 (MQ=255)
                                                                                                                 gctgctGCCAGCGGGAATAATTACGGATTTTGGCATCGTTAAACTCCTTAAGCGATATGAGCAAAGGACGTGGGAGAAAGCGCGTCGCAG‑‑AATGTTTCGACGTCGCTGACCCAGCCAAAAAGGGTTTCGATATTGCACAACGCGGCttt                                >  1:568910/1‑149 (MQ=255)
                                                                                                                      tgcCAGCGGGAATAATTACGGATTTTGGCATCGTTAAACTGCTTAAGCGATATGAGCAAAGGACGTGGGAGAAAGCGCGTCGCAG‑‑AATGTTTCGACGTCGCTGACCCAGCCAAAAAAGGTTTCGATATTGAACAACGCGGCTTTCTgcg                           <  1:155285/149‑1 (MQ=255)
                                                                                                                      tgcCAGCGGGAATAATTACGGATTTTGGCATCGTTAAACTCCTTAAGCGATATGAGCAAAGGACGTGGGAGAAAGCGCGTCGCAG‑‑AATGTTTCGACGTCGCTGACCCAGCCAAAAAAGGTTTCGATATTGAACAACGCGGCTTTCTgcg                           <  1:850503/149‑1 (MQ=255)
                                                                                                                        ccAGCGGGAATAATTACGGATTTTGGCATCGTTAAACTCCTTAAGCGATATGAGCAAAGGACGTGGGAGAAAGCGCGTCGCAG‑‑AATGTTTCGACGTCGCTGACCCAGCCAAAAAAGGTTTCGATATTGAACAACGCGGCTTTc                               >  2:1085669/1‑143 (MQ=255)
                                                                                                                        ccAGCGGGAATAATTACGGATTTTGGCATCGTTAAACTCCTTAAGCGATATGAGCAAAGGACGTGGGAGAAAGCGCGTCGCAG‑‑AATGTTTCGACGTCGCTGACCCAGCCAAAAAAGGTTTCGATATTGAACAACGCGGCTTTCTGCGCa                         >  1:604052/1‑149 (MQ=255)
                                                                                                                              ggAATAATTACGGATTTTGGCATCGTTAAACTCCTTAAGCGATATGAGCAAAGGACGTGGGAGAAAGCGCGTCGCAG‑‑AATGTTTCGACGTCGCTGACCCAGCCAAAAAAGGTTTCGATATTGAACAACGCGGCTTTCTGCGCAAATTTc                   <  1:216774/149‑1 (MQ=255)
                                                                                                                              ggAATAATTACGGATTTTGGCATCGTTAAACTCCTTAAGCGATATGAGCAAAGGACGTGGGAGAAAGCGCGTCGCAG‑‑AATGTTTCGACGTCGCTGACCCAGCCAAAAAAGGGTTCGATATGGAACAACGCGGCTTTCTGCGCAAGTTTc                   >  1:435952/1‑149 (MQ=255)
                                                                                                                               gAATAATTACGGATTTTGGCATCGTTAAACTCCTTAAGCGATATGAGCAAAGGACGTGGGAGAAAGCGCGTCGCAG‑‑AATGTTTCGACGTCGCTGACCCAGCCAAAAAAGGTTTCGATATTGAACAACGCGGCTTTCTGCGCaa                        >  2:87617/1‑143 (MQ=255)
                                                                                                                               gAATAATTACGGATTTTGGCATCGTTAAACTCCATAAGCGATATGAGCAAAGGACGTGGGAGAAAGCGCGTCGCAG‑‑AATGTTTCGACGTCGCTGACCCAGCCAAAAAGGTTTCGATATTGAACAACGCGGCTTTCTGCGCaaa                       >  2:922946/1‑143 (MQ=255)
                                                                                                                                aataatTACGGATTTTGGCATCGTTAAACTCCTTAAGCGATATGAGCAAAGGACGTGGGAGAAAGCGCGTCGCAG‑‑AATGTTTCGACGTCGCTGACCCAGCCAAAAAAGGTTTCGATATTGAACAACGCGGCTTTCTGCGCAAATTTCgg                 >  1:537890/1‑149 (MQ=255)
                                                                                                                                aataatTACGGATTGTTGCATCGTTCAACTCCTTAAGCGATATGAGCAAAGGACGTGGGAGAAAGCGCGTCGCAG‑‑AATGTTCCGACGTCGCTGACCCAGCCAAAAAAGGTTTCGATATTCAACAACGCGGCTTTCTGCGCAAATTTCgg                 <  1:793904/149‑1 (MQ=255)
                                                                                                                                aagaatTACGGATTTTTGCATCGTTAAACTCCTTAAGCGATATGAGCAAAGGACGTGGGAGAAAGCGCGTCGCAG‑‑AATGTTTCGACGTCGCTGACCCAGCCAAAAAAGGTTTCGATATTGAACAACGCGGCTTTCTGCGCAAATTTCgg                 <  1:147212/146‑1 (MQ=255)
                                                                                                                                               tGGCATCGTTAAACTCCTTAAGCGATATGAGCAAAGGACGTGCGAGAAAGCGCGTCGTAG‑‑AATGTTTCGACGTCGCTGACCCAGCCAAAAAAGGTTTCGATATTGAACAACGCGGCTTTCTGCGCAAATTTCGGCCCCGCCTg        >  2:802067/1‑143 (MQ=255)
                                                                                                                                               tGGCATCGTTAAACCCCTTAAGCGATATGAGCAAAGGACGTGGGAGAAAGCGCGTCGCAG‑‑AATGTTTCGACGTCGCTGACCCAGCCAAAAAAGGTTTCGATATTGAACAACGCGGCTTTCTGCGCAAATTTCGGCCCCGCCTGGTGAGt  <  1:291595/149‑1 (MQ=255)
                                                                                                                                               tGGCATCGTGAAACTCCTCAGGCGATATGAGCAAAGGACGTGGGAGAAAGCGCGTCGCAG‑‑AATGTTTCGACGTCGCTGACCCAGCCAAAAAAGGTTTCGATATTGAACAACGCGGCTTTCTGCGCAAATTTCGGCCCCGTCTg        <  2:775547/143‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                     
GCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAGCGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTGGCATCGTTAAACTCCTTAAGCGATATGAGCAAAGGACGTGGGAGAAAGCGCGTCGCAG‑‑AATGTTTCGACGTCGCTGACCCAGCCAAAAAAGGTTTCGATATTGAACAACGCGGCTTTCTGCGCAAATTTCGGCCCCGCCTGGTGAGT  >  CP009273/1067467‑1067758

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: