| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 766,135 | G→T | 6.9% | intergenic (+68/‑779) | mngB → / → cydA | alpha‑mannosidase/cytochrome d terminal oxidase, subunit I |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 766,135 | 0 | G | T | 6.9% | 162.1 / 3.9 | 58 | intergenic (+68/‑779) | mngB/cydA | alpha‑mannosidase/cytochrome d terminal oxidase, subunit I |
| Reads supporting (aligned to +/- strand): ref base G (24/30); new base T (2/2); total (26/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.37e-01 | |||||||||||
AAATCAAGGTTCAAATCTATCGGGGCCTTTTTTACCCGGCCAGTCACGGACGTTCAGTTACCGGCTTGCCTGAATAGCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATGAACGCTTTATTCGGTCTACAAAAGCAGGCAAATTCAATATATTGCAGAGATGATGTAGACACTGGCAAGCGTAGCGCATCAGGTAATTTTGCGTTTATCTTCACTCTCAAGCCACGTATATGTGGCTTTATTTTTAAC > CP009273/765995‑766283 | aaaTCAAGGTTCAAATCTATCGGGGCCTTTTTTACCCGGCCAGTCACGGACGTTCAGTTACCGGCTTGCCTGAATAGCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCa < 1:228318/149‑1 (MQ=255) caTCAAGGTCCAAAACTATCGGCGCCTTTTTTACCCAGCCCGTCACGGACGTTCAGTTACCGGCTTGCCTGAATAGCAATCAAACCGAATCCACATATGCGCGGCCAGATTGTTGACAAAAGGCGCATTGTTCACGCCGGATa < 2:72224/142‑1 (MQ=255) cAAGGTTCAAATCTATCGGGGCCTTTTTTTTTTTTTTTTTCACGGACGTTCAGTTACCGGCTTGCCTTACTAGCAATCAAACCGAAGCCACATATGCGCCGCCAGATTGTTGACAAAGGGCGCTTTGTTCCTTCCGGATACgg > 2:1152593/1‑143 (MQ=255) tCAAATCTATCGGGGCCTTTTTTACCCGGCCAGTCACGGACGTTCAGTTACCGGCTTGCCTGAATAGCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGTATCCGGCATGaa < 2:447865/143‑1 (MQ=255) tCAAATCTAGCGGGGCCTTTTTTGCCCGGCCAGTCACGGACGTTCAGTTACCGGCTTGCCTGAATAGCAATTGAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGTATCCGGCATGaa < 2:879267/143‑1 (MQ=255) aaaTCTATCGGGGCCTTTTTTACCCGGCCAGTCACGGACGTTCAGTTACCGGCTTGCCTGAATAGCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATGCACg < 2:570999/143‑1 (MQ=255) cTATCGTGTCCTTTTTTACCCGGCCAGTCACGGACGTTCAGTTACCGGCTTCCCTGAATAGCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTTACAAAGGGCGCTTTGTTCATGCCGGATACGGCATGAACGCTTTATTCgg < 1:77426/149‑1 (MQ=255) gaTCGGGGCCTTTTTCACCCGGCCAGTCACGGACGTTCCGTTACCGGCTTGCCTGAATAGCAATCCAACCGAAGCCACATCTGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATGAACGCTTTa < 2:513693/142‑1 (MQ=255) ccGGGGCCTTTTTTACCCGGCCAGTCACGGACGTTCAGTTACCGGCTTGCCTGAATAGCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATGAACGCTTTATTCGGTCt < 1:402543/148‑1 (MQ=255) gtgCCTGTTTTACCCGGCCAGTCACGGACGTTCAGTTACCGGCTTGCCTGAATAGCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATGAACGCTTTATTCgg < 2:354440/141‑1 (MQ=255) gggCCTTTTTTCCCCGGCCAGTCACGGACGTTCAGTTACCGGCTTGCCTGAATAGCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATGAACGCTTTATTCgg < 2:417891/143‑1 (MQ=255) ggCCTTTTTTACCCGGCCAGTCACGGACGTTCAGTTACCGGCTTGCCTGAATAGCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATGAACGCTTTATTCGGt < 2:963622/143‑1 (MQ=255) ggCCTTGTGTACCCGGCCAGGCACGGACGTTCAGTTACCGGCTTGCCTGAATAGCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATGAACGCTTTATTCGGTCTACaa < 1:23065/149‑1 (MQ=255) ttttttACCCGGCCAGTCACGGATGTTCAGTTACCGGCTTGCCTGAATAGCAAGCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATGAACGCTTTATTCGGTCTACAAAAGc > 1:714717/1‑149 (MQ=255) ttttACCCGGCCAGTCACGGACGTTCAGTTACCGGCTTGCCTGAATAGCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATGAACGCTTTATTCGGTCTACaa < 2:1134033/143‑1 (MQ=255) cccGGCCAGTCACGGACGTTCAGTTACCGGCTTGCCTGAATAGCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATGAACGCTTTATTCGGTCTACAAAAGCAGGCaaa < 1:465487/149‑1 (MQ=255) cGGCCAGTCACGGACGTTCAGTTACCGGCTTGCCTGAATAGCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATGAACGCTTTATTCGGTCTACAAAAGCAgg < 2:76097/143‑1 (MQ=255) cGGCCAGTCACGGACGTTCAGTTACCGGCTTGCCTGAATAGCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATGAACGCTTTATTCGGTCTACAAAAGCAgg < 2:75875/143‑1 (MQ=255) ccAGTCACGGACGTTCAGTTACCGGCTTGCCTGAATAGCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATGAACGCTTTATTCGGTCTACAAAAGCAGGCAAATTCaa < 1:1290745/149‑1 (MQ=255) aGTCACGGACGTTCAGTTACCGGCTTGCCTGAATAGCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATGAACGCTTTATTCGGTCTACAAAAGCAGGCAAATTCaaga > 1:804013/1‑147 (MQ=255) aGTCACGGACGTTCAGGTACCGGCTTGCCCGAATAGCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTACATGCCGGATACGGCATGAACGCTTTATTCGGTCGACAAAAGCAGGCAAATTCAata > 1:825191/1‑149 (MQ=255) gTCACGGAGGTCCTGTTACGTGCTTGCCTGAATAGCAATCAACCCGAAGCCACAGATGCGCGGCCAGATTGTTGACAAAGGGCGCTTGGTTCATGCCGGATACGGCATGAACGCTTTATTCGGTCTACAAAAGCAGGCAAATTCAatat < 1:492293/149‑1 (MQ=255) cACGGACGTTCAGTTACCGGCTTGCCTGAATAGCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATGAACGCTTTATTCGGTCTACAAAAGCAGGCAAATTCa < 2:939685/143‑1 (MQ=255) cGGACGTTCAGTTACCGGCTTGCCTGAATAGCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATGAACGCTTTATTCGGCCTACAAAAGACGGCAAATTCAat > 2:1057626/1‑143 (MQ=255) cAGTTACCGGCTTGCCTGAATAGCAATCAAACCGAAGCCACATATGCGCCGCCAGATTGTCGACAAAGGGCGATTTGTTCATGCCGGATACGGCATGAACGCTTTATTCGGCCTACAAAAGCAGGCAAATTCAATTTATTTCa < 2:382172/143‑1 (MQ=37) ttACCGGCTTGCCTGAATAGCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATGAACGCTTTATTCGGTCTACAAAAGCAGGCAAATTCAATATATTGCagag > 2:101126/1‑143 (MQ=255) cGGCTTGCCTGAATAGCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATGAACGCTTTATTCGGTCTACAAAAGCAGGCAAATTCAATATATTGCAGAgatga > 2:815399/1‑143 (MQ=255) gattgaatgtaTAGCAATCAAACCGAAGCCACATATGCGCGGGCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATGAACGCTTTATACGGTCTACAAAAGCAGGCAAATGCAATATATTGCCGAgatgagg > 2:750811/11‑141 (MQ=37) cTGAATAGCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATGAACGCTTTATTCGGTCTACAAAAGCAGGCAAATTCAAAAAATTGCAGAGATAATGTAGaca > 2:137645/1‑143 (MQ=255) aTAGCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATGAACGCTTTATTCGGTCTACAAAAGCAGGCAAATTCAATATATTGCAGAGATGATGTAGACACTgg > 2:910921/1‑143 (MQ=255) aTAGCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATGAACGCTTTATTCGGTCTACAAAAGCAGGCAAATTCAATATATTGCAGAGATGATGTAGACACCgg > 2:444910/1‑143 (MQ=255) aaTCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATGAACGCTTTATTCGGTCTACAAAAGCAGGCAAATTCAATATATTGCAGAGATGATGTAGACACTGGCAAGCGTAgcg > 1:1136276/1‑149 (MQ=255) aaaCCGAAGCCACATATGCGCGGCGAGATTGTTGACAAAGGGCGCTTGGTTCATGCCGGATACGGCATGAACGCTTTATTCGGTCTACAAAAGCAGGCAAATTCAATATATTGCAGAGATGATGTAGACACTGGCAGGCGTAGCGCagg > 1:674802/1‑147 (MQ=255) aCCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATAAGGCATGAACGCTTTATTCGGTCTACAAAAGCAGGCAAACTCAATACATTGCAGAGATGATGTAGGCACTGGGAAGCGTAgcg > 2:677502/1‑143 (MQ=25) gcGAAGCCACATATGTGCGGTCAGATTGTTGACAAGGGGCGCGTTGTTCAGGCCGGATACGGCATGAACGCTTTATTCGGTCTACAAAAGCAGGCAAATTCAATATATTGCAGAGATGATGTAGACACTGGCAAGCGTAGCGCATCAgg < 1:49712/148‑1 (MQ=37) ccGACGCCCCATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATGAACGCTTTATTCGGTCTACAAAAGCAGGCTAATTCAATATATTGCAGACATGATCTAGATACTGGCAAGCGTAGCGCATCAgg < 1:718264/149‑1 (MQ=38) ggtggCCAGATTGTCGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATGAACGCTTTATTCGGTCTACACCAGCAGGCAACTTCAATACATTGCAGAGTTGCTGTAGACGCTAGCAAGCGGAGCGCATCGGGTAATTTGGc < 2:659700/140‑1 (MQ=255) gcgGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATGAACGCTTTATTCGGTCTACAAAAGCAGGCAAATTCAATATATTGCAGAGATGATGTAGACACTGGCAAGCGTAGCGCATCAGGTAATTTTGCg < 2:1006715/143‑1 (MQ=255) cgGCCAGATTGTTGACAGAGGGCGCTTTGTTCATGCCGGATACGGCATGAACGCTTTATTCGGTCTACAAAAGCAGGCAAATTCAATATATTGCAGAGATGATGTAGACACTGGCAAGCGTAGCGCATCAGGTAATTTTGCGTTTATCt < 1:712073/149‑1 (MQ=255) gCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATGAACGCTTTATTCGGTCTACAAAAGCAGGCAAATTCAATATATTGCAGAGATGATGTAGACACTGGCAAGCGTAGCGCATCAGGTAATTTTGCGttt < 2:695326/143‑1 (MQ=255) gCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCACGAACGCTTTATTCGGTCTACAAAAGCAGGCAAATTCAATATATTGCAGAGATGATGTAGACACTGGCAAGCGTAGCGCATCAGGTAATTTTGCGTTTATCTTc < 1:1153191/149‑1 (MQ=255) ccAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATGAACGCTTTATTCGGTCTACAAAAGCAGGCAAATTCAATATATTGCAGAGATGAGGTAGACACTGGCAAGCGTAGCGCATCAGGTAATTTTGCGTTTATCTTCa > 1:1009129/1‑149 (MQ=255) agcttgttgGCAAAGGGCGCTGTGTTCATGCCCGATCCGGCATGAACGCTTTATTCGGTCTACAAAAGCAGTCAAATTCAATTTATTGCAGAGATGATGTAGACACTGGCAAGCGTAGCGCATCAGGTAATTTTGCGTTTATCTTCAct < 1:987912/146‑1 (MQ=255) aCAAAGGGCGCTTTGTTCATGCCGGATACGGCATGAACGCTTTATTCGGTCTACAAAAGCAGGCAAATTCAATATATTGCAGAGATGATGTAGACACTGGCAAGCGTAGCGCATCAGGTAATTTTGCGTTTAAATTCAAtctc > 2:452267/1‑143 (MQ=255) accgaataaagcgTTCATGCCGTATCCGGCATGAACGCTTTATTCGGTCTACAAAAGCAGGCAAATTCAATATATTGCAGAGATGATGTAGACACTGGCAAGCGTAGCGCATCAGGTAATTTTGCGTTTATCTTCACTCTCAAACCACg > 1:464843/13‑149 (MQ=37) aGGGCGCTTTGTTCATGCCGGATACGGCATGAACGCTTTATTCGGTCTACAAAAGCAGGCAAATTCAATATATTGCAGAGATGATGTAGACACTGGCAAGCGTAGCGCATCAGGTAATTTTGCGTTTATCTTCACTCTCAAGCCACgga > 1:29259/1‑147 (MQ=255) tttGTTCATGCCAGATACGGCATGAACGCTTTATTCGGTCTACAAAAGCAGGCAAATTCAATATATTGCAGAGATGATGTAGACACTGGCAAGCGTAGCGCATCAGGTAATTTTGCGTTTATCTTCACTCTCAAGCCACGTATATGTgg > 1:938680/1‑149 (MQ=255) gtttttCATGCCGGTTACGGCATTAACGCTTTATTCGGTCTACAAAAGCAGGCAAATTCAATATATTGCAGAGATGATGTAGACACTGGCAAGCGTAGCGCATCAGGTAATTTTGCGTTTATCTTCACTCTCAAGCCACGTATATGTgg < 1:418491/145‑1 (MQ=255) tGTTCATGCCGGATACGGCATGAACGCTTTATTCGGTCTACAAAAGCAGGCAAATTCAATATATTGCAGAGATGATGTAGACACTGGCAAGCGTAGCGCATCAGGTCATTTTGCGTTTATCTTCACTCTCAAGCCACGTATAAGTGGCt > 1:280812/1‑149 (MQ=255) gTTCATGCCGGATACGGCATGAACGCTTTATTCGGTCTACAAAAGCAGGCAAATTCAATATATTGCAGAGATGATGTAGACACTGGCAAGCGTAGCGCATCAGGTACTTTTGCGTTTATCTTCACTCTCAAGCCACGTATATGTGGCtt > 1:199423/1‑149 (MQ=255) ttCATGCCGTATCCGGCATGAACGCTTTATTCGGTCTACAAAAGCAGGCAAATTCAATATATTGCAGAGATGATGTAGACACTGGCAAGCGTAGCGCATCAGGTAATTTTGCGTTTATCTTCACTCTCAATCCACGTATAtgt > 2:50073/1‑143 (MQ=255) gtCATGCCGGATACGGCATGAACGCTTTATTCGGGCTACAAAAGCAGGCAAATTCAATATATTGCAGAGATGATGTAGACACTGGCAAGCGTAGCGCACCAGGTAATTTTGCGTTTATCTTCACTCTCACACCACGTATAtgt < 2:413444/142‑1 (MQ=255) tCATGCCGGATACGGCATGAACGCTTTATTCGGTCTACAAAAGCAGGCAAATTCTATATATTGCAGAGATGATGTAGACACTGGCAAGCGTAGCGCATCAGGTAATTTTGCGTTTATCTTCACTCTCAAGCCACGTATAtgtg > 2:694847/1‑143 (MQ=255) tCATGCCGGATACGGCATGAACGCTTTATTCGGTCTACAAAAGCAGGCAAATTCAATATATTGCAGAGATGATGTAGACACTGGCAAGCGTAGCGCATCAGGTAATTTTGCGTTTATCGTCACTCGCAAGCCACGTATAtgtg > 2:998092/1‑143 (MQ=255) gCCGGATACGGGATGAACGCTTTATTCGGTCTACAAAAGCAGGCAAATTCAATATATTGCAGAGATGATGTAGACACTGGCAAGCGTAGCGCATCAGGTAATTTTGCGTTTATCTTCACTCTCAAGCCACGTATATGTGGCtt < 2:73012/143‑1 (MQ=255) gCCGGATACGGCATGAACGCTTTATTCGGTCTACAAAAGCAGGCAAATTCAATATATTGCAGAGATGATGTAGACACTGGCAAGCGTAGCGCATCAGGTCATTTTGCGTTTATGTTCACTCTCAAGCCACGTATCTGTGGCtc > 2:934632/1‑142 (MQ=255) gCCGGATACGGCATGAACGCTTTATTCGGTCTACAAAAGCAGGCAAATTCAATATATTGCAGAGATGATGTAGACACTGGCAAGCGTAGCGCATCAGGTAATTTTGCGTTTATCTTCACTCTCGAGGCACGCTAATGTGGCtt > 2:934771/1‑143 (MQ=37) ccGGATACGGCATGAACGCTTTATTCGGTCTACAAAAGCAGGCAAATTCAATATATTGCAGAGATGATGTAGACACTGGCAAGCGTAGCTCATCAGGTAATTTTGCGTTTATCTTCACTCTCAAGCCACGTATATGTGGCTTTAttttt < 1:864551/149‑1 (MQ=255) gATACGGCATGAACGCTTTATTCGGTCTACAAAAGCAGGCAAATTCAATATATTGCAGAGATGATGTAGACACTGGCAAGCGTAGCGCATCAGGTAATTTTGCGTTTATCTTCACTCTCAAGCCACGTATATGTGGCTTTATTTTTAAc > 1:402852/1‑149 (MQ=255) gATACGGCATGAACGCTTTATTCGGTCTACAAAAGCAGGCAAATTCAATATATTGCAGAGATGATGTAGACACTGGCAAGCGTAGCGCATCAGGTAATTTTGCGTTTATCTTCACTCTCAAGCCACGTATATGTGGCTTTATTTTTAAc > 1:642383/1‑149 (MQ=255) | AAATCAAGGTTCAAATCTATCGGGGCCTTTTTTACCCGGCCAGTCACGGACGTTCAGTTACCGGCTTGCCTGAATAGCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATGAACGCTTTATTCGGTCTACAAAAGCAGGCAAATTCAATATATTGCAGAGATGATGTAGACACTGGCAAGCGTAGCGCATCAGGTAATTTTGCGTTTATCTTCACTCTCAAGCCACGTATATGTGGCTTTATTTTTAAC > CP009273/765995‑766283 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |