Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,519,265:1 +A 3.9% intergenic (+15/+24) yfeN → / ← yfeR putative outer membrane protein/transcriptional regulator of yefH

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,519,2651.A 3.9% 424.6 / 11.3 102intergenic (+15/+24)yfeN/yfeRputative outer membrane protein/transcriptional regulator of yefH
Reads supporting (aligned to +/- strand):  ref base . (57/41);  new base A (2/2);  total (59/43)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.69e-02

Only 100 of 105 total aligned reads displayed.

TGGCGCAGTTGCACTGTGGTGGAATGCAACATCACACATTACTACGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTGCGCACCAGC  >  CP009273/2519118‑2519409
                                                                                                                                                    |                                                                                                                                                
tGGCCCAGTTTCAATGGGGTGGAATGCAACATCACATATTACTACGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCgggg                                                                                                                                                 <  1:191271/149‑1 (MQ=255)
    gcAGTTGCACTGTGGTGGAATGCAACATCACACATTACTACGGGGATTCAGTACCGATATGGGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACATCTGATTGATTACTGCCGGGGCTcc                                                                                                                                             >  1:796532/1‑149 (MQ=255)
     cAGTTGCACTGTGGTGGAATGCAACATCACACATTACTACGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCggg                                                                                                                                                   <  2:40073/143‑1 (MQ=255)
        ttGCACTGTGGTGGAATGCAACATCACACATTACTACGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCt                                                                                                                                               >  2:980109/1‑143 (MQ=255)
         tGCACTGTGGTGGAATGCAACATCACACATTACTACGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCa                                                                                                                                        <  1:1226941/149‑1 (MQ=255)
          gCACTGTGGTGGAATGCAACATCACACATTACTACGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGAGCCCCGGCAg                                                                                                                                       <  1:1100593/149‑1 (MQ=255)
           cACTGTGGTGGAATGCAACATCACACATTACTACGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGCATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTccc                                                                                                                                            >  2:461974/1‑143 (MQ=255)
             cTGTGGTGGAAGGCAACATCACACCTTACTACGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACGCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTAt                                                                                                                                    <  1:1203494/149‑1 (MQ=255)
             atttagtagaagacAACATCACACATTACTACGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTAt                                                                                                                                    <  1:1087885/136‑1 (MQ=255)
              tgtgGTGGAATGCAACATCACACATTACTGCGGGGATTCAGTATCGATATGCGAATGACAAGCTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGACTCCCGGCAGTATg                                                                                                                                   >  1:795724/1‑149 (MQ=255)
                tggtggAATGCAACATCACACATTACTACGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGaa                                                                                                                                 >  1:812477/1‑149 (MQ=255)
                     gAATGCAACATCACACATTACTACGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTCCTGCCGGGGCTCCCGCCAGTCTGa                                                                                                                                  >  2:822718/1‑143 (MQ=255)
                       aTGCAACATCACACATTACTACGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCActct                                                                                                                         >  1:1259892/1‑149 (MQ=255)
                        tGCAACATCACTCATGTCTACGGGGATTCAGTATCGGTATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGAGCCCCGGCAGTaatcaat                                                                                                                               <  2:308412/143‑7 (MQ=255)
                        cgaaaCATCACACATTACTACGGGGGTTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAAGATGCAtt                                                                                                                               >  2:1053518/4‑143 (MQ=255)
                          cAACATCACACATTCCTACGGGGATACAGTATCTATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAAGCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCTGGGCTCCCGGCAGTATGAATTCACTCtat                                                                                                                       <  1:793047/149‑1 (MQ=255)
                          cAACATCACACATTACTACGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCTATGCAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCtat                                                                                                                       >  1:623443/1‑149 (MQ=255)
                            aCATCACACATTGCTACGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCAct                                                                                                                           <  2:773647/143‑1 (MQ=255)
                                  cacaTTACTACGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATa                                                                                                               <  1:481393/149‑1 (MQ=255)
                                      ttACTACGGGTATTCAGTATCGATATGCTGATTACTATCTGGGTGAAGATTTTTACCAGGATGCGATAATCTACCCCATCAAATTTAACTTCTGATTGATTACTGGCGGGGCTCCCGGCAGTATTAATTCACTCTATATCTGATACAAc                                                                                                           <  1:376034/149‑1 (MQ=255)
                                      ttACTACGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGa                                                                                                                 <  2:793293/143‑1 (MQ=255)
                                      ttACTACGGGGATTCAGTATCGATATGCGGAGGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTgc                                                                                                                 >  2:792069/1‑142 (MQ=255)
                                         cttcGGGCATTCAGTATCGATATCCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATTATTTCACTCTATATCTGATACAACGGa                                                                                                        <  1:446035/146‑1 (MQ=255)
                                         ctacGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGa                                                                                                        <  1:428123/149‑1 (MQ=255)
                                           acGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCCGTGCGGATTCACCCTATATCCGTGACAACGGacg                                                                                                      >  1:109099/1‑147 (MQ=255)
                                            cGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATcc                                                                                                     >  1:1083937/1‑149 (MQ=255)
                                                  ttCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCCCTCTATATCTGATACAACGGATCCCCTTcc                                                                                               >  1:5611/1‑149 (MQ=255)
                                                  ttCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTcc                                                                                               >  1:478713/1‑149 (MQ=255)
                                                   tCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATccc                                                                                                    <  2:850554/143‑1 (MQ=255)
                                                   tCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGAAGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTCTGCATTCACTCTAAATCTGCTACAACGGATccc                                                                                                    >  2:315270/1‑143 (MQ=255)
                                                    cggTATCGAGATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATcccc                                                                                                   <  2:623587/141‑1 (MQ=255)
                                                    cAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCg                                                                                             >  1:419061/1‑149 (MQ=255)
                                                     aGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGc                                                                                            <  1:599655/149‑1 (MQ=255)
                                                        aTCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGACAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCa                                                                                         >  1:800342/1‑149 (MQ=255)
                                                         tCGATATGCGGATGACAAGCTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTACATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTACATCTGATACAACGGATCCCCTccca                                                                                              >  2:727903/1‑142 (MQ=255)
                                                          cGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGc                                                                                       >  1:1221617/1‑149 (MQ=255)
                                                            atatGCCGAAGACTAACCTGGCGAAAATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGTGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGcc                                                                                           <  2:734492/143‑1 (MQ=255)
                                                             tatCCGGATGACAAACTGGGCGAAGATTTTTAGCAGGATGCGATCATCTACTCCATCAAATTTAACTTCTGATCGATTACTGCCGGGGCGCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCCTCCCGccc                                                                                          >  2:814014/1‑143 (MQ=255)
                                                              atGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCAtt                                                                                   >  1:1239437/1‑149 (MQ=255)
                                                              aggCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCa                                                                                         <  2:941191/141‑1 (MQ=255)
                                                                 cGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAgc                                                                                >  1:1157979/1‑149 (MQ=255)
                                                                    aTGAAAAGCGGGGCGAAGAATATTACCAGGATGCGATAAAATACACCACCTAAGTTAACTGCCGATTGGTCGCTTCCGGTGCCAAACGCAGTATTAAGGCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCa                                                                             <  1:953373/149‑1 (MQ=255)
                                                                            cTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTg                                                                     >  1:783911/1‑149 (MQ=255)
                                                                               ggCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGcc                                                                        >  2:431181/1‑143 (MQ=255)
                                                                                 cGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGtctc                                                                <  1:640903/149‑1 (MQ=255)
                                                                                  gAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTCGCGCATTGCCCTg                                                                     >  2:901928/1‑143 (MQ=255)
                                                                                  gAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCg                                                               >  1:1235433/1‑149 (MQ=255)
                                                                                    aGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTa                                                             >  1:710562/1‑149 (MQ=255)
                                                                                       tttttACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCa                                                          <  1:286236/149‑1 (MQ=255)
                                                                                         tttccccgaaTGCGATAATCTACTCCATCAAATTTCACTTCTGCTTGATTACTGCCGGGGCTCCCGGCAGTATGCATTCACTCTATATCGGATACAACGGCTCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGt                                                              <  2:1127638/134‑1 (MQ=255)
                                                                                         tttACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCGCCCGGCAGTATGCATTCTCTCTATATCTGATAACACGGATCCCCTTCCCGCCCAGCCATCAGCGCATTGCCCTGGtcgctg                                                              >  2:271541/1‑139 (MQ=255)
                                                                                           tACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTc                                                      <  1:514394/149‑1 (MQ=255)
                                                                                                tgttGCGATAATCTACTCCATCAAACTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATa                                                 <  1:301/146‑1 (MQ=255)
                                                                                                 gATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATaa                                                >  1:1289687/1‑149 (MQ=255)
                                                                                                 gATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATaa                                                <  1:605800/149‑1 (MQ=255)
                                                                                                  aTGCGATAACCTACTCCATCAAATTTACCTCCGGATTGACTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATaaa                                               <  1:120186/149‑1 (MQ=255)
                                                                                                   tGCGATACTCTACGCCATCAAATGTAACTTCTGATTGGTTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTccc                                                    <  2:397975/143‑1 (MQ=255)
                                                                                                   tGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATACCTGATACAACGGATCCCCTCCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTccc                                                    <  2:397815/143‑1 (MQ=255)
                                                                                                     cGACAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCCTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCAt                                                  <  2:904384/143‑1 (MQ=255)
                                                                                                             tACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATCAAGCTTc                                          >  2:390267/1‑143 (MQ=255)
                                                                                                             tACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAACGCTTc                                          >  2:1031422/1‑143 (MQ=255)
                                                                                                                tCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGt                                 >  1:1023685/1‑149 (MQ=255)
                                                                                                                atcATAAAATTTAACTTCTGTTTGCGTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGt                                 <  1:939866/147‑1 (MQ=255)
                                                                                                                  taTCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTgg                               <  1:320138/148‑1 (MQ=255)
                                                                                                                  cATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTgg                               >  1:831453/1‑149 (MQ=255)
                                                                                                                   aTCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGATTCAGCGGCGGTGGa                              >  1:572701/1‑149 (MQ=255)
                                                                                                                    tCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGaa                             >  1:10064/1‑149 (MQ=255)
                                                                                                                    tCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGaa                             >  1:1195578/1‑149 (MQ=255)
                                                                                                                     cAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTCTATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGGGGGGGaaa                            >  1:339624/1‑149 (MQ=255)
                                                                                                                        ctttAACTTCTGATTGATTACTGCCGGGCCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTgg                               <  2:790586/142‑1 (MQ=255)
                                                                                                                           tAACTTCTGATTGATTACTGCCGGGGTTCCCGGCAGTGTGAATACCCTCTATATCTGTTTCATCGGCTCCCCTTCCCTCCCAGCCATGAGCGCATTGCCCTGCTCTCGTACCACGTCCCATATAGCTTCAGCGGCGGtcgtaa                            <  2:94372/143‑6 (MQ=255)
                                                                                                                           tAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGaaa                            <  2:703260/143‑1 (MQ=255)
                                                                                                                             aCTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACgg                    <  1:408154/149‑1 (MQ=255)
                                                                                                                              cTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGt                   >  1:768278/1‑149 (MQ=255)
                                                                                                                              cTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCACTGAAGCTTCAGCGGCGGGGGAAAGTg                         >  2:996416/1‑143 (MQ=255)
                                                                                                                               ttCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGATTCAGCGGCGGGGGAAAGTGAACGGtt                  >  1:560186/1‑149 (MQ=255)
                                                                                                                               ctCTGATTGAATGCTGCCGGGGCTCCCGGGAGTATGAATTCGCTCTATATCTGATACAACGGAGCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTTGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGtt                  <  1:495474/148‑1 (MQ=255)
                                                                                                                                 cTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCGGCCATCAGCGCATTGCCCTGGTCTCTTCCCACGTCCCATAAAGCTGCAGCCGCGGTGGACAGTGAAc                      >  2:737047/1‑143 (MQ=255)
                                                                                                                                  tgattgatTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAGGTGAAGGGttctt               >  1:666734/1‑149 (MQ=255)
                                                                                                                                   gattgattACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACgg                    >  2:22189/1‑143 (MQ=255)
                                                                                                                                   gattgattACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGttcttc              <  1:537917/149‑1 (MQ=255)
                                                                                                                                   gattgattACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGACGAAAGTGAAggg                    >  2:795574/1‑143 (MQ=255)
                                                                                                                                   gattgattACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCGTCCCGCGCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACgg                    >  2:344871/1‑143 (MQ=255)
                                                                                                                                    attgattACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAGGCTTCAGCGGCGGTGGGAAGTGAACGTTTCTTcc             >  1:3130/1‑149 (MQ=255)
                                                                                                                                    attgattACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGt                   >  2:557311/1‑143 (MQ=255)
                                                                                                                                    attgattACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTcc             >  1:654468/1‑149 (MQ=255)
                                                                                                                                     ttgattACTGCCGGGGCCCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCAAGCCAAATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCt            <  1:634236/149‑1 (MQ=255)
                                                                                                                                        attACTGCCGGGGCTCCCGGCAGTATGTATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGTATTGCCCTGGTCTCGTACCACGTCCCATAAAGCGTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTgcg         <  1:368131/149‑1 (MQ=255)
                                                                                                                                        attACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGGTCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGAGGTGGAAAGTGAACGGTTCTTCCTgcg         >  1:739534/1‑149 (MQ=255)
                                                                                                                                        attACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGttctt               >  2:1093783/1‑143 (MQ=255)
                                                                                                                                         ttACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTgcgc        >  1:788145/1‑149 (MQ=255)
                                                                                                                                         atACTGCCGGGAGCCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTgcgc        >  1:322513/2‑149 (MQ=255)
                                                                                                                                           accgCCGGGGCCCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCt            <  2:453333/140‑1 (MQ=255)
                                                                                                                                           aCTGCCGGGAGCCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGGTCTTCCt            >  2:879169/1‑143 (MQ=255)
                                                                                                                                           aCTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCt            <  2:361868/143‑1 (MQ=255)
                                                                                                                                             tGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTGCGCACCa    >  1:165433/1‑149 (MQ=255)
                                                                                                                                              gCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTGCGCACCAg   <  1:638797/149‑1 (MQ=255)
                                                                                                                                               ccGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAGCGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACAGCCCATAAAGCGTCGGCGGCGGTGGAAAGTGAACGGTTCTTCCTGCGCAcaggc  >  1:123011/1‑145 (MQ=255)
                                                                                                                                               ccGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTGGCGCATTGCCCTGGTCTCGTACCACGTCCCTTCAAGCTTCACCCGCGGTGGAAAGGGACGGGTTCTTCCTgcgc        >  2:964533/1‑143 (MQ=255)
                                                                                                                                               ccGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTGCGCACCAGc  >  1:1109928/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                
TGGCGCAGTTGCACTGTGGTGGAATGCAACATCACACATTACTACGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTGCGCACCAGC  >  CP009273/2519118‑2519409

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: