| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 463,920 | Δ1 bp | 30.4% | coding (82/459 nt) | ybaO → | putative DNA‑binding transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 463,920 | 0 | G | . | 30.4% | 114.8 / 66.6 | 56 | coding (82/459 nt) | ybaO | putative DNA‑binding transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base G (14/25); new base . (6/11); total (20/36) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.02e-01 | |||||||||||
ATAAATTGTCGTGATTTCACCTTTAAAATAAAATTAAAAGAGAAAAAAATTCTCTGTGGAAGGGCTATGTTAGATAAAATTGACCGTAAGCTGCTGGCCTTACTGCAGCAGGATTGCACCCTCTCTTTGCAGGCACTGGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGAGGACGACGGTATCCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGCCTCGGCCTGACCGCTTTTGTGCTGATAAAAACGCAA > CP009273/463773‑464057 | aTAAATTGTCGTGATTTCACCTTTAAAATAAAATTAAAAGAGAAAAAAATTCTCTGTGGAAGGGCTATGTTAGATAAAATTGACCGTAAGCTGCTGGCCTTACTGCAGCAGGATTGCACCCTCTCTTTGCAGGCACTGGCTGAAGCCGt < 1:855480/149‑1 (MQ=255) gTGATTTCACCTTTAAAATAAAATTAAAAGAGAAAAAAATTCTCTGTGGAAGGGCTATGTTAGATAAAATTGACCGTAAGCTGCTGGCCTTACTGCAGCAGGATTGCACCCTCTCTTTGCAGGCACTGGCTGAAGCC‑TTAATCTGACaa > 1:1015050/1‑149 (MQ=255) gTGATTTCACCTTTAAAATAAAATTAAAAGAGAAAAAAATTCTCTGTGGAAGGGCTATGTTAGATAAAATTGACCGTAAGCTGCTGGCCTTACTGCAGCAGGATTGCACCCTCTCTTTGCAGGCACTGGCTGAAGCC‑TTAATCTGACaa > 1:1011070/1‑149 (MQ=255) tgctgtCACCTTTAAAACAAAATTAAAAGGGAAAAAAATTCTCGGCGGAAGGGCTATGTTAGGTAAAATTGACCGTAAGCTGCTGGCCTTACTGCAGCAGGATTGCCCCCTCTCTTTGCAGGCACTGGCTGAAGCCGTTAATCTGACaa < 1:881490/144‑1 (MQ=255) ttCACCTTTAAAATAAAATTAAAAGAGAAAAAAATTCTCTGTGGAAGGGCTATGTTAGATAAAATTGACCGTAAGCTGCTGGCCTTACTGCAGCAGGATTGCACCCTCTCTTTGCAGGCACTGGCTGAAGCC‑TTAATCTGACAaccacc < 1:797110/149‑1 (MQ=255) tttAAAATAAAATTAAAAGAGAAAAACCAGTTTTGTGGAAGGGCTATGTTAGATAAAATTGACCGTAAGCTGCTGGCCTTACTGCAGCAGGATTGCACCCTCTCTTTGCAGGCACTGGCTGAAGCCGTTAATCTGccacccac > 2:807861/1‑143 (MQ=255) tatgaaatacttttAAAAGAGAAAAAAAGTCTCTGTGGAAGGGCTATGTTAGATAAAATTGACAGTAAGCTGCTGGCCTTACTGCAGCAGGATTGCACCCTCTCTTTGCAGGCACTGGCTGCAGCCGCTAACCTGACAgccac > 2:751015/13‑143 (MQ=255) aaaataaaatTAAAAGAGAAAAAAATTCTCTGTGGAAGGGCTATGTTAGATAAAATTGACCGTAAGCTGCTGGCCTTACTGCAGCAGGATGGCACCCCCTCCTTGCATGCACTGGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTg < 1:309586/149‑1 (MQ=255) aataaaatTAAAAGAGAAAAAAAGTCTCTGTTGAAGGGCTATGTAAGATAAAATTGACCGTAAGCTGCTGGTCGTACTGCAGCAGGATTGCACCCTCTCTTTGCAGGCACTGGCTGAAGCC‑TTAGTCTGACAACCACCCCTTg < 2:66790/143‑1 (MQ=255) aaaaTAAAAAAAAAAAAAATTCTCTGTGGAAGGGCTATGTTAGATAAAATTGACCGTAAGCTGCTGGCCTTACTGCAGCAGGATTGCACCCTCTCTTTGCAGGCACTGGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTg < 2:784977/143‑1 (MQ=255) catTAAAAGAGAAAAAAATTCTCTGTGGAAGGGCTATGTTAGATAAAATTGACCGTAAGCTGCTGGCCTTACTGCAGCAGGATTGCACCCTCTCTTTGCAGGCACTGGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAgcgc < 1:1021341/148‑1 (MQ=255) atTAAAAGAGAAAAAAATTCTCTGTGGAAGGGCTATGTTAGATAAAATTGACCGTAAGCTGCTGGCCTTACTGCAGCAGGCTTGCACCCTCTCTTTGCAGGCACTGGCTGAAGCC‑TTAATCTGACAACCACCCCTTGCTGGaa < 2:463094/143‑1 (MQ=255) aaaGAGAAAAAAATTCTCTGTGGAAGGGCTATGTTAGATAAAATTGACCGTAAGCTGCTGGCCTTACTGCAGCAGGATTGCACCCTCTCTTTGCAGGCACTGGCTGAAGCCGTTAATCTGACAACCACGCCTTGCTGGAAGCGCCTGaa > 1:1153248/1‑149 (MQ=255) gagaAAAAAATTCTCTGTGGAAGGGCTATGTTAGATAAAATTGACCGTAAGCTGCTGGCCTTACTGCAGCAGGATCGCACCCTCCCTTTGCAGGAACTGCCTGGAGCCGTGAATCTGACAACCACCCCTTCCTGGAAGCGCCt > 2:953634/1‑143 (MQ=255) agaAAAAAATTCTCTGTGGAAGGGCTATGTTAGATAAAATTGACCGTAAGCTGCTGGCCTTACTGCAGCAGGATTGTACCCTCTCTTTGCAGGCACTGTCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTg > 2:98879/1‑143 (MQ=255) aaaaaTTCTCTGTGGAAGGGCTATGTTAGATAAAATTGACCGTAAGCTGCTGGCCTTACTGCAGCAGGATTGCACCCTCTCTTTGCAGGCACTGGCTGAAGCC‑TTAATCTGACAACCGCCCCTTGCTGGAAGCGCCTGAAACGGCTgga < 1:751173/149‑1 (MQ=255) ctctGTGGAAGGGCTATGTTAGATAAAATTGACCGTAAGCTGCTGGCCTTACTGCAGCAGGATTGCACCCTCTCTTTGCAGGCACTGGCTGAAGCCGTTAATCTGACAACCACCCCTTCCTGGAAGCGCCTGAAACGGCTGGAGgacga < 1:678159/149‑1 (MQ=255) tcggtgGAAGGGCTAGGTTAGATAAAATTGACCGTAAGCTGCTGGCCTTACTGCAGCAGGATTGCACCCTCTCTTTGCAGGCACTGGCTGAAGCC‑TTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTggagg < 2:804396/140‑1 (MQ=255) tgtgGAAGGGCTATGTTAAATAAAATTGACCGTAAGCTGACGGTTTTACTGCCGCAGGACGGTGGCGTCTCGGGTCAGTCACTGGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTggagga > 2:801450/1‑143 (MQ=255) tggggAAGGGCTATGTTATATATAATTGACCGTAAGCTGCTGGCCTTACTGCAGCAGGATTGCACCCTCTCTTTGCAGGCACTGGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTggagga < 2:476577/140‑1 (MQ=255) ggAAGGGCTATGTTAGATAAAATTGACCGTAAGCTGCTGGCCTTACTGCAGCAGGATTGCACCCTCTCTTTGCAGGCACTGGCTGAAGCC‑TTAATCTGACAACCACCCCTTGCTGGAGGCGGCTGAAACGGCTGGATgtcgac < 2:1113004/143‑1 (MQ=255) aaGGGCTATGTTAGATAAAATTGACCGTAAGCTGCTGGCCTTACTGCAGCAGGATTGCACCCTCTCTTTGCAGGCACTGGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGAGGACGACGGTATcc < 1:154460/149‑1 (MQ=255) gggCTATGTTAGATAAAATTGACCGTAAGCTGCTGGCCTTACTGCAGCAGGATTGCACCCTCTCTTTGCAGGCACTGGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGCAAGCGCCTGAAACGGCTGGAGGACGACGGt < 2:468185/143‑1 (MQ=255) gggCTATGTTAGATAAAATTGACCGTAAGCTGCTGGCCTTACTGCAGCAGGATTGCACCCTCTCTTTGCAGGCACTGGCTGAAGCC‑TTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGAGGACGACGGTa > 2:893133/1‑143 (MQ=255) gCTCTGTTAGATAAAAGTGACCGTAAGCTGCTGGCCTTACTGCAGCAGGATTGCACCCTCTCTTTGCAGGCACTGGCTGAAGCC‑TTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGAGGACGACGGTATc < 2:575253/143‑1 (MQ=255) tATGTTAGATAAAATGGGCCGTAAGCTGCGGGCCTTACTGCAGCAGGATTGCACCCTCTCTTTGCAGGCACTGGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGAGGACGACGGTATCCTTATCg < 1:110072/149‑1 (MQ=255) gTTAGGTAAAATTGACCGTAAGCTGCTGGCCTTACTGCAGCAGGATTGCACCCTCTCTTTGCAGGCACTGGCTGAAGCC‑TTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGAGGACGACGGTATCCTTAt < 2:931317/143‑1 (MQ=255) gTTAGACAAAATTGACCGTAAGCTGCTGGCCTTACTGCAGCAGGATTGCACCCTCTCTTTGCAGGCACTGGCTGAAGCC‑TTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGAGGACGACGGTATCCTTAt < 2:714749/143‑1 (MQ=255) atatgggccGTAAGCTGCTGGCCTTACTGCAGCAGGATTGCACCCTCTCTTTGCAGGCACTGGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGAGGACGACGGTATCCTTATCGGCaaa < 2:364989/136‑1 (MQ=255) aaaTTGACCGTAAGCTCCTGGCCTTACTGCAGCAGGATTGCACCCCTTCTTTGCAGGCACTGGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGAGGACGACGGTATCCTTATCGGCaaa < 2:668429/143‑1 (MQ=255) gTAAGCTGCCGGCCTTACGGCAGCAGGATTGCACCCCCTCTTTGCAGGCACTGGCTGAAGCC‑TTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGAGGACGACGGTATCCTTATCGGCAAAGTCGCCctgc < 2:453093/143‑1 (MQ=255) gctgctGGCCTTACTGCAGCAGGATTGCACCCTCTCTTTGCAGGCACTGGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGAGGACGACGGTATCCTTATCGGCAAAGTCGCCCTGCTgg < 2:342429/143‑1 (MQ=255) gctgctGGCCTTACTGCAGCAGGATTGCACCCTCTCTTTGCAGGCACTGGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGAGGACGACGGTATCCTTATCGGCAAAGTCGCCCTGCTgg > 2:430985/1‑143 (MQ=255) gctgctGGCCTTACTGCAGCAGGATTGCACCCTCTCCTTGCAGGCACTGGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGAGGACGACGGTATCCTTATCTGCAAAGTCGCCCTGCTGGATCCgg < 1:971041/149‑1 (MQ=255) gctgcgGGCCCTCCTGCAGCAGCATTGCACCCTCCCTTTGCAGGCACTGGCTGAAGCCGTTAATCTGACAACCACCCCATGCTGGAAGCGCCTGAAACGGCTGGAGGACGACGGTATCCTTATCGGCAAAGCCGCCCTGCTGGATCCgg < 1:277971/149‑1 (MQ=255) ctgctgGCCTTACTGCAGCAGGATTGCACCCTCTCTTTGCAGGCACTGGCTGAAGCC‑TTAATCTAGCAACGACAACTTGCTGGAAGCGCCTGAAACGGCTGGAGGACGACGGGATCCTCATCGGCACAGTCGCCCTGCCGGAt > 2:979699/1‑143 (MQ=255) ggCCTGACTGCAGCAGGATTGCACCCTCTCTTTGCAGGCACGGGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGCAACGCCTGGAGGACGACGGTATCCTTATCGGCAAAGTCGCCCTGCTGGATCCGGaaaaaa < 1:44013/149‑1 (MQ=255) ccgtccTGGAGTAGGCTTGCACCCTCTCTTTGCAGGCACTGGCAGAAGCCGTCAATCTGACAACCACCCCTCGCTGGAAGCGCCTGAAACGGCTGGAGGACGACGGTATCCTCATCGGCAAATTCGCCCTGCTGGATCCGGaa < 2:1054854/138‑1 (MQ=255) ttGCACCCTCTCTTTGCAGGCACTGGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGAGGACGACGGTATCCTTATCGGCAAAGTCGCCCTGCTGGATCCGGCAAAAATACGCCCCGGcc > 2:1036153/1‑143 (MQ=255) ttGCACCCTCTCTTTGCAGGCACTGGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGAGGACGACGGTATCCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGCCTCGGCCTGACCg > 1:1080006/1‑149 (MQ=255) ttaCCCTCTCTTTGCAGCCACTGGCTGAAGCCGTTAACCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGAGGACGACGGTATCCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGCCTCGGCCTGACCGCt < 1:1263262/147‑1 (MQ=255) cccTCTCTTTGCAGGCACTGGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCAGGAGGACGACGGTATCCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATCGGCCTCGGCCTGACCGCtttt > 1:529049/1‑149 (MQ=255) ccTCTCTTTGCAGGCACTGGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGAGGACGACGGTATCCTTATCGGCAAAGTCGCCCTGCTGGATCCGGCAAAAATAGGCCTCGGCCTGACCGCTTTtg > 1:585882/1‑149 (MQ=255) ccTCTCTTTGCAGGCACTGGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGAGGACGACGGTATCCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGCCCCGGCCTGAccc > 2:2544/1‑142 (MQ=255) ccTCTCTTTGCAGGCACTGGCTGAAGCC‑TTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGAGGACGACGGTATCCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGCCTCGGACTGACCGc > 2:1034184/1‑143 (MQ=255) ctctctTTGCAGGCACTGGCTGAAGCC‑TTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGAGGACGACGGTATCCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGCCTCGGCCTGACCGCt > 2:546933/1‑143 (MQ=255) tctctTGGCAGGCACTGGCTGAAGCCGTTAATCTGACAACCACCCGTTGCTGGAAGCGCCTGAAACGGCTGGAGGACGACGGTATCCTTATCGGCAAAGTCGCCCTGCTGGATCCGGGAAAAATGGGCCTCGGCCTGACCGCt > 2:557948/1‑143 (MQ=255) ttGCAGGCACTGGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGAGGACGACGGTATCCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGCCTCGGCCTGACCGCTTTTGTGCTGAt > 1:1231758/1‑149 (MQ=255) gCAGGCACTGGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGAGGACGACGGTATCCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGCCTCGGCCTGACCGCTTTTGTGCTGATaa < 1:983658/149‑1 (MQ=255) cAGGCACTGGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGAGGACGACGGTATCCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGCCTCGGCCTGACCGCTTTTGTGCt < 2:1005991/143‑1 (MQ=255) tgCACTGGCGGAAGCCGGCAATGTGACAGCCGCCCCTTGCTGGAAGCGCCTGAAGCGGCTGGAGGACGACGGTATCCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGCCTCGGCCTGACCGCTTTTGTGCTGa < 2:111909/142‑1 (MQ=255) gCACTGGCTGAAGCCGTTAACCTGACCACCGCCCCTTGCCGGAAGCGCCTCAAACGGCTGGAGGACGACGGTATCCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGCCTCGGCCTGACCGCTTTTGTGTTGATAAAAAc < 1:496902/149‑1 (MQ=255) aatGGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAATCGGCTGGAGGACGACGGTATCCTTATCGGCAAAGTCGCCCTGCTGGATCCGGATAAAATAGGCCTCGGCTTGACCGCTTTTGTGCTGATaa < 2:155334/141‑1 (MQ=255) cTGGCTGAAGCC‑TTAATCTGACAACCCCCCCTTGCTGGAAGCGCCTGAAACGGCTGGAGGACGACGGTATCCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGCCTCGGCCTGACCGCTTTTGTGCTGATAAAAACGCaa < 1:873678/149‑1 (MQ=255) aacccGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGAGGACGACGGTATCCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGCCTCGGCCTGACCGCTTTTGTGCTGATAAAAACGCaa < 2:1016703/140‑1 (MQ=255) aaGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGAGGACGACGGTATCCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGCCTCGGCCTGACCGCTTTTGTGCTGATAAACTCGCaa < 2:698112/143‑1 (MQ=255) | ATAAATTGTCGTGATTTCACCTTTAAAATAAAATTAAAAGAGAAAAAAATTCTCTGTGGAAGGGCTATGTTAGATAAAATTGACCGTAAGCTGCTGGCCTTACTGCAGCAGGATTGCACCCTCTCTTTGCAGGCACTGGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGAGGACGACGGTATCCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGCCTCGGCCTGACCGCTTTTGTGCTGATAAAAACGCAA > CP009273/463773‑464057 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |