Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,208,0070TG23.4% 150.5 / 11.7 77S534S (TCT→TCG) rpoDRNA polymerase, sigma 70 (sigma D) factor
Reads supporting (aligned to +/- strand):  ref base T (28/31);  new base G (0/18);  total (28/49)
Fisher's exact test for biased strand distribution p-value = 1.27e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

AACGTATGCTGATGCCGGAAGACAAGATCCGCAAAGTGCTGAAGATCGCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTGGGTAAACAGTTCGACGTTACCC  >  CP009273/3207859‑3208155
                                                                                                                                                    |                                                                                                                                                    
aaCGTATGCTGATGCCGGAAGACAAGATCCGCAAAGTGCTGAAGATCGCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCt                                                                                                                                                      >  2:311617/1‑149 (MQ=255)
 aCGTATGCTGATGCCGGAAGACAAGATCCGCAAAGTGCTGAAGATCGCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTg                                                                                                                                                     <  1:365261/149‑1 (MQ=255)
  cggatggcgaggCCGGAAGACAAGATCCGCAAAGTGCCGAAGGTGGGCAAAGAGCCAATCTGCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGc                                                                                                                                                    <  1:219091/138‑1 (MQ=255)
  cGTATGCTGATGCCGGAAGACAAGATCCGCAAAGTGCTGAAGATCGCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGc                                                                                                                                                    <  1:849708/149‑1 (MQ=255)
       gctggcggcGGAAGACGAGCTCCGCAAAGTGCTCAAGATCGGCAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGacca                                                                                                                                               <  1:712359/141‑1 (MQ=255)
        cTGATGCCGGAAGACAAGATCCGCAAAGTGCTGAAGATCGCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAGGATTCGCATCTGGGGGATTTCATCGAGGGTACCACCCTCGAGCTGCCGCTGGATTCTGCGaccac                                                                                                                                              >  1:277468/1‑149 (MQ=255)
           aTGCCGGAAGACAAGATCCGCAAAGTGCTGAAGATCGCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGa                                                                                                                                           >  2:789446/1‑149 (MQ=255)
              ccGGAAGACAAGATCCGCAAAGTGCTGAAGATCGCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGaagg                                                                                                                                        >  2:375263/1‑147 (MQ=255)
                 gAAGACAAGATCCGCAAAGTGCTGAAGATCGCAAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCCAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCt                                                                                                                                     <  2:436730/149‑1 (MQ=255)
                     aCAAGCACCCCAAGGTTCTGAAGACCGCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGTGTTGTTTCATGATACGGCGACCACCGAGAGCCtgcgt                                                                                                                                 <  1:803080/149‑1 (MQ=255)
                     aCAAGATCCGCAAAGTGCTGAAGATCGCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCtgcgt                                                                                                                                 >  2:58162/1‑149 (MQ=255)
                       aagtgccGCAAAGTGCTGAAGAGCGCCAAAGAGCCCATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCTCATCTGGGGGATTTCATCGAGGATACCACCCTTTTTTTTTTTAATGATTCGGCGACCACCGAGAGCCtgcgtgc                                                                                                                               <  1:741383/144‑1 (MQ=255)
                       aaGATCCGCAAAGTGCTGAAGATCGCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGCTTTCATCGAGGATACCACCCTCGACCTGCCGCTGGATTCTGCGACCACCGAAAGCCggcgtgc                                                                                                                               >  1:689608/1‑149 (MQ=255)
                         gATCCGCAAAGTGCTGAAGGTCGCCAAAGGGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCgg                                                                                                                             <  2:454186/149‑1 (MQ=255)
                         gATCCGCAAAGTGCTGAAGATCGCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGCTACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCgg                                                                                                                             >  1:805594/1‑149 (MQ=255)
                          aTCCGCAAAGTGCTGAAGATCGCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGc                                                                                                                            >  2:722338/1‑149 (MQ=255)
                          aTCCGCAAAGTGCTGAAGATCGCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGc                                                                                                                            >  2:382592/1‑149 (MQ=255)
                            ccGCAAAGTCGCGAAGATCGCCAAAGCGCCAATCTCCATGGAACCGCCGATCGGTTATGATGAAGATTCGCATCTGGGGGATTTCACCGAGGATACCACCCTCGTTCTTTCTCATGATTCTGCGACCACCGAGAGCCTGCGTGCGGCaa                                                                                                                          <  1:166413/149‑1 (MQ=255)
                                   gTGCTGATGGTCGCCAAGGGGCCAAGCTCGTTGGTGACGCCGATCGGTGATGTTGAAGTTTCGCATCTGGGGGATTTCATCGAGGATACCACCGTTTTGCTGTTGCATGATTCGGCGACCACCGAGAGCCTGCGTGCGGCAACGCacga                                                                                                                   <  1:6341/149‑1 (MQ=255)
                                   gTGCTGAAGATCGCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGCTTCGCATCTGGGGGATTTCATCGAGGATACCACCCTTTTTTTGTTTAAGGATTCGGCGACCACCGAGAGCCTGCGTGCGGCAACGCacga                                                                                                                   <  1:714882/149‑1 (MQ=255)
                                         aaGATCGCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTgct                                                                                                             <  2:884762/149‑1 (MQ=255)
                                             tCGCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGTTGCCGCAGGATTCGGCGACCACCGAGAGCCTGCGTGCGGCAACGCACGACGTgctggct                                                                                                         <  1:352726/149‑1 (MQ=255)
                                               gCCAAAGCGCCAATCCCCATGGAACCGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGTGTTTCCTCATGATTCGGCGACCACCGAGAGCCTGCGTGCGGCAACGCACGACGTGctggctgg                                                                                                       <  1:755556/149‑1 (MQ=255)
                                                ccAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCtggctggc                                                                                                      <  2:76785/149‑1 (MQ=255)
                                                 cAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCCCATCTGGGGGATTTCATCGAGGATACCACCCTCGAACTGCCGCTGGATTCTGCGACCACCGCAAGCCTGCGTGCGGCAACCCACGACGTGCTGGCTGGcc                                                                                                     >  2:363392/1‑149 (MQ=255)
                                                           aTCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGCCcgcgcg                                                                                           >  2:158488/1‑149 (MQ=255)
                                                             ccccAGGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTCGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTg                                                                                         <  2:540597/147‑1 (MQ=255)
                                                             ccccAAGGAACCGCCAAACGGCGAGTGTGGAGATGCGCATCTGGGGGATTCCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTg                                                                                         <  2:825413/147‑1 (MQ=255)
                                                              tcCATGGAACCGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTTTTGTTTTTTAATGATACGGCGACCACCGAGAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGa                                                                                        <  1:719480/149‑1 (MQ=255)
                                                               gcATGGCTACGCCGTTTGGCGATGTTGCAGTTTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCTTTTTTTTTATTGATACGGCGACCACCGAGAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGaa                                                                                       <  1:11790/148‑1 (MQ=255)
                                                               ccATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCTTGCTAGTTCGTGCTGGCTGGCCTGACCGCGCGTGaa                                                                                       <  2:436961/149‑1 (MQ=255)
                                                                cATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGGCGTGCTGGCTGGCCTGCCCGCGCGCGAAg                                                                                      >  1:843393/1‑149 (MQ=255)
                                                                   gtaaccgctgcacGGTGATGAGGAAGACTCGCATCGGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCAACGACGCCCCGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCaa                                                                                   <  2:175417/137‑1 (MQ=255)
                                                                    caaaCGCCGCTCGGTGAGGATCAACATCTGCATCTGGGGGATTTCATTGAAGCTACCCCCCTTTTTTTTTTGAATGATACGGCGACCACCGAGAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCaaa                                                                                  <  1:270257/148‑1 (MQ=255)
                                                                     aaaCGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCaaaa                                                                                 <  2:237136/149‑1 (MQ=255)
                                                                          ccGGTCGGGGATGAGGGGGATTCGCATCTCGGGGATTGCATCGAGGAGACCACGCTTTTTTTTTTTCATGATACGGCGACCACCGAGAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCt                                                                            <  1:168441/149‑1 (MQ=255)
                                                                           cGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTg                                                                           <  2:56942/149‑1 (MQ=255)
                                                                             aTCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCg                                                                         <  2:79575/149‑1 (MQ=255)
                                                                             aTCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCg                                                                         <  2:522562/149‑1 (MQ=255)
                                                                                  tgatgatgaAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGc                                                                    <  2:391165/149‑1 (MQ=255)
                                                                                      gatgaAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGttt                                                                <  2:845711/149‑1 (MQ=255)
                                                                                      gatgaAGACTCGTCTTTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGTCGAATGATTCGGCGACCACCGAGAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGttt                                                                <  1:361235/149‑1 (MQ=255)
                                                                                       atgaAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTc                                                               >  2:96544/1‑149 (MQ=255)
                                                                                       atgaAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGGGCTGGCGCGCCTGACCGCGCGTGAAACAACAGGTCCGCGGATGCGTGTc                                                               >  1:572272/1‑149 (MQ=255)
                                                                                        tgaAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCg                                                              <  2:220308/149‑1 (MQ=255)
                                                                                          aaGCTTCGCATCTGGGGGCTTTCATCGAGGCTACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGt                                                            <  2:741943/149‑1 (MQ=255)
                                                                                           aGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGATGAATTCTGCGCCCACCGAAAGCCTGCGTGCGGCCACGGGCGACGTGCTGGCTGGCCTGACCGCGGGTGACGCAAGAGGTCTGCGTGTGCGTTTCGGTa                                                           >  2:237495/1‑149 (MQ=255)
                                                                                            gATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTAt                                                          >  2:887568/1‑149 (MQ=255)
                                                                                            gATTCGCATCTGGGGCATTTCATCGAGGATACCACCCGCGAGCTGCCGCTGGATTCGGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTAt                                                          <  1:631965/149‑1 (MQ=255)
                                                                                             aTTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATc                                                         >  2:597629/1‑149 (MQ=255)
                                                                                                  cATCTGGGGGATTTCATCGAGGATACCACCCTCGGGTTGCCGCTGGATTCGGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGatat                                                    <  1:219838/149‑1 (MQ=255)
                                                                                                  cATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGGGTGTGCGTTTTGGTATCgttgt                                                    >  2:15982/1‑145 (MQ=255)
                                                                                                    tCTGGGGGACTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGa                                                  <  2:295343/149‑1 (MQ=255)
                                                                                                     cTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGaa                                                 <  2:681568/149‑1 (MQ=255)
                                                                                                      tGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATCCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGCCGTCCTGGCCGGCCGGACCGCGCGTGAAGCAAAAGTCCTGCACACGCGGTACGGTGTCGGTAGGagc                                                >  1:258363/1‑147 (MQ=255)
                                                                                                        ggggCTTTCATGGAGGATACCACCCTCGCGCTGTTGCAGGATACGGCGACCACCGAGAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAacac                                              <  1:115872/149‑1 (MQ=255)
                                                                                                            aTTTTATCGAGGATACTACCCTCGTGTTGCCGCTGGATTCGGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGAc                                          <  1:214652/149‑1 (MQ=255)
                                                                                                             tttCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGAACCGGCTGGCCTGACCGCGCGTGAAGCAAAAGAACTGCGTATGCGTTCCGGAACCGATCTGCACACCGACt                                         >  1:346253/1‑149 (MQ=255)
                                                                                                               tCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCAGGACGTGCTGGCTGGCCTGACCACGCGTGAAGCAAAAGTACTGCGGATGCGTTTCGCTAGCGATAGGAACACCGCCtgc                                       >  1:1238/1‑147 (MQ=255)
                                                                                                                cATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTaca                                      >  2:112722/1‑149 (MQ=255)
                                                                                                                  tCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAGCGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCCAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACg                                    >  2:150406/1‑149 (MQ=255)
                                                                                                                     aGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGCCTACACGCTg                                 >  2:237202/1‑149 (MQ=255)
                                                                                                                     aGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTg                                 <  2:744967/149‑1 (MQ=255)
                                                                                                                      ggATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTgg                                <  2:805594/149‑1 (MQ=255)
                                                                                                                       gATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGGAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACCACACGCTGga                               >  2:385705/1‑149 (MQ=255)
                                                                                                                          accaccCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCGGGCCTGACCGCGCGTGAAGCAAAAGTTATGCGGATGAGTTTCGGTATCGATACGAAGACCGCCTAAGCGCCGgacaa                            >  1:174399/1‑146 (MQ=255)
                                                                                                                            caccCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTCTGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGaagaag                          >  2:634453/1‑149 (MQ=255)
                                                                                                                            caccCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGaagaag                          <  2:146452/149‑1 (MQ=255)
                                                                                                                             accCTCGAGCCGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGt                         <  2:113459/149‑1 (MQ=255)
                                                                                                                               ccTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCCACCCACGGCGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTgg                       <  2:249693/149‑1 (MQ=255)
                                                                                                                                cccGAGCTGCCGCTGGATTCTGCAACCACCGACAGCCTGTGGGCGGCAACGCACGACGTGCTGGCCGGCCTGCCCGCGCGTGAAGCAAAAGTTCTGCTTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTggg                      <  2:361623/147‑1 (MQ=255)
                                                                                                                                 ttggggttccGCTGGATGCGGCGACCACCGAGAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTGGGt                     <  1:224250/141‑1 (MQ=255)
                                                                                                                                         ccGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTGGGTAAACAGtt             <  2:769925/149‑1 (MQ=255)
                                                                                                                                         ccGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTGGGTAAACAGtt             >  2:680250/1‑149 (MQ=255)
                                                                                                                                             tGGATTCGGCGCCCCCCGAGAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTGGGTAAACAGTTCGAc         <  1:722338/149‑1 (MQ=255)
                                                                                                                                             gtgATTCGGCGACCACCGAGAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGACCACCTGGTACTCGCTGGAAGAAGTGGGTAAACAGTTCGAc         <  1:414408/147‑1 (MQ=255)
                                                                                                                                                 ttCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTGGGTAAACAGTTCGACGTTa     >  2:154515/1‑149 (MQ=255)
                                                                                                                                                   cTGCGACCCCCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTGGGTAAACAGTTCGACGTTAcc   <  1:382592/149‑1 (MQ=255)
                                                                                                                                                    tGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTTGCCGCGCGTGAAGCAAAAGTTCTGCGTAAGCGTCTCGGTACCGCTATGAACACCCACTACACGCGGGAGGCAGTGGGTGAACAGCTCAACGTTAccc  >  1:111490/1‑149 (MQ=255)
                                                                                                                                                    tGCGACCACCGAAAGCCTGCGTGCGGCAAAGCACAACGTGCTGGCAGGCCTTGCGGCAGGTGACGCAAAACTTAAACGCATGCGATTCCGGATCGATAGGAACACGGACAACACGCTGGCATAAGCGGGTAAGCAGTCGGACGCCAccc  >  1:442443/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
AACGTATGCTGATGCCGGAAGACAAGATCCGCAAAGTGCTGAAGATCGCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTGGGTAAACAGTTCGACGTTACCC  >  CP009273/3207859‑3208155

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑