Predicted mutation
evidence seq id position mutation freq annotation gene description
JC JC CP009273 2,400,257 IS5 (+) +4 bp 43.5% intergenic (‑137/‑780) lrhA ← / → alaA transcriptional repressor of flagellar, motility and chemotaxis genes/valine‑pyruvate aminotransferase 2

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? CP009273 2282398 =NA (NA)6 (0.290) 6/276 NT 30.9% noncoding (1195/1195 nt) IS5 repeat region
?CP009273 2400257 = 13 (0.660)intergenic (‑137/‑783) lrhA/alaA transcriptional repressor of flagellar, motility and chemotaxis genes/valine‑pyruvate aminotransferase 2
* ? CP009273 = 2283592NA (NA)14 (0.690) 13/276 NT 51.1% noncoding (1/1195 nt) IS5 repeat region
?CP009273 = 2400260 13 (0.660)intergenic (‑140/‑780) lrhA/alaA transcriptional repressor of flagellar, motility and chemotaxis genes/valine‑pyruvate aminotransferase 2

AGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTAAAAGCATTGGGTACAGAAAATACCCATAGCTCCATAC  >  CP009273/2283452‑2283729
                                                                                                                                            |                                                                                                                                         
aGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCt                                                                                                                                   >  2:338393/1‑149 (MQ=35)
  atacgctgttgtCTGGTCGGACGGCGCTGCGTCCTGAACTCGCCGTCGGCTAAGGTAAGTCGCTGACGTATGATCACCCCGCTTCGGTGGATCCATATGACCACCCTGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGt                                                                                                                                 <  1:240528/138‑1 (MQ=2)
             tcgggTCTGGCGGCGCTTACTTCTTAATTCACTGTCGGAGAAGGTAAGTTGATGACTCATGCTGAACCCTGTTCTATGCCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGaa                                                                                                                      <  1:116187/146‑1 (MQ=14)
               tGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACa                                                                                                                    >  2:47754/1‑149 (MQ=255)
                    tGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCGCCAGAACAGatat                                                                                                               >  2:96024/1‑149 (MQ=255)
                           gcTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGaa                                                                                                        <  2:144244/149‑1 (MQ=255)
                                                          aaGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCa                                                                         <  1:40008/149‑1 (MQ=255)
                                                             ttGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGAACACGAGAAACAGCACCAGATTGAAGCATTg                                                                      >  1:145232/1‑149 (MQ=21)
                                                                       aggatgaACCTTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATc                                                            <  1:139328/147‑1 (MQ=255)
                                                                         gatgaACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTGCGCCCCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGGCAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCt                                                          <  1:319349/149‑1 (MQ=255)
                                                                                       tATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACGGAACCAGATTGATGCATTGTGCTTTCATCCTATGAAATTAATTGc                                            >  1:135575/1‑149 (MQ=255)
                                                                                        aTGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCt                                           <  2:240100/149‑1 (MQ=255)
                                                                                                     aCAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTAAAAGCATTg                              >  2:318758/1‑149 (MQ=255)
                                                                                                       aaaCATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTAATCCTATGAAATTAATTGCTGTTAAAAGCATTagg                            >  1:104423/1‑149 (MQ=255)
                                                                                                              aTCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTAAAAGCATTGGGTACAGaa                     <  2:195315/149‑1 (MQ=255)
                                                                                                               tctcATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTCTGAAATTAAATGCTGTTAAAAGCATTTGGTCCAGaaa                    >  1:147066/1‑149 (MQ=255)
                                                                                                                    tatCAGGGACTTTTTCCCACCGTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTAAAAGCATTGGGTACAGAAAATAcc               <  1:29028/149‑1 (MQ=255)
                                                                                                                                 tccGCACCTGCCATAACCCCACAGCCACCAGAACAGATATTGCGGAACGACAAAGACAAACATAACTAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTCAAAACCATTGGGTACAGAAAATACCCATCGCTcactcc  <  1:140878/147‑6 (MQ=255)
                                                                                                                                            |                                                                                                                                         
AGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTAAAAGCATTGGGTACAGAAAATACCCATAGCTCCATAC  >  CP009273/2283452‑2283729

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.