Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP009273	2,718,735	G→T	P124Q (CCG→CAG)	kgtP ←	alpha‑ketoglutarate transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	2,718,735	0	G	T	100.0%	164.2 / NA	55	P124Q (CCG→CAG)	kgtP	alpha‑ketoglutarate transporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (22/33);  total (22/33)

GTCCGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCGGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACACATCATACACACCGATAACAGCATCGATTTTTTGCGACCATGTTTATCGGCTATGCGGCCAAATAGCCAACCGCCTATTGGGC  >  CP009273/2718589‑2718879                                                                                                                                                   |                                                                                                                                                 tcccgccgGTCAACGTCACATACTGAAATGACGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAACGCCTGa                                                                                                                                                <  1:76273/147‑1 (MQ=255)  tCCGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCTGAg                                                                                                                                               <  1:86114/149‑1 (MQ=255)  cccgccgATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCTGAg                                                                                                                                               <  1:86229/148‑1 (MQ=255)               gTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCACCTTAACACATATAGGTGGCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGCGACGCAATCATGCCTGAGCCCCCGTACCaat                                                                                                                                  >  1:155534/1‑149 (MQ=255)               gTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCTGAGCCCACGt                                                                                                                                        >  2:169374/1‑143 (MQ=255)                  acaTACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCTGAGCCCACGTACCTATAGt                                                                                                                               <  1:179648/149‑1 (MQ=255)                    aTACTGAAATGATGCGTAAAACCCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCTGAGCCCACGTACCTATAGttt                                                                                                                             <  1:614007/149‑1 (MQ=255)                    aTACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATTGGTGGCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCTGAGCCCACGTACCta                                                                                                                                   <  2:354024/143‑1 (MQ=255)                          aaaTGATGCGTAAAAAGCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGAGGCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCTGAGCCCACGTACCTATAGttt                                                                                                                             >  2:499600/1‑143 (MQ=255)                            atgatgCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCTGAGCCCACGTACCTATAGTTTCATAACCt                                                                                                                     <  1:102280/149‑1 (MQ=255)                                 gCGTAAAAACCTTTGCGCCCTTCTACGGCAACCTCACTCATATAGGTGGCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCTGAGCCCACGTACCTATAGTTTCATAAcc                                                                                                                      <  2:445778/143‑1 (MQ=255)                                     aaaaaCCTTGGCGCCCTTCAACGGCAACTTCACTCATATAGCTGTCGCGGGTGCCAGATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAAAGCCTGAGCCCACGTACCTATAGTTTCATAACCTGGGaggcag                                                                                                            <  1:611397/149‑1 (MQ=255)                                      aaaaCCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCTGAGCCCACGTACCTATAGTTTCATAACCTGGGa                                                                                                                 >  2:532419/1‑143 (MQ=255)                                        aaCCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCCGCCAACAGACAATCCCTCAAAGAAACGAGCGAGAAGCAATAATGCCTGAGCCCACGTACCTATAGTTTCATAACCTGGGagg                                                                                                               <  2:40037/143‑1 (MQ=255)                                             ttGCGCCCTTCAACGGCAACTTCTCTCATATAGGTGGGGCTGGTGCCATATTCTCCGCCAACAGTTAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCTGAGCCCACGTACCTATAGTTTCATAACCTGGGaggcaggc                                                                                                          <  2:262444/143‑1 (MQ=255)                                               gcgcCCTTCAACGGCAACTTCACTCAGATAGGTGGCGCTGGGGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCTGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCa                                                                                                  <  1:367237/149‑1 (MQ=255)                                                 gcCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCTGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGc                                                                                                >  1:55771/1‑149 (MQ=255)                                                     ttCAACGGCAACTTCACTCATATAGGTGGCTCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCTGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATc                                                                                            >  1:387701/1‑149 (MQ=255)                                                     gtCAACGGCAACTTCAGTCATATAGGTGGCGCGGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGAGAGAAGCAGTAATGCCTGAGCCCACGTACCTATAGTGTCATAACCTGGGAGGCAGGGGGTAACCa                                                                                                  >  2:167066/2‑143 (MQ=255)                                                      tCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCTGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATcc                                                                                           <  1:642580/149‑1 (MQ=255)                                                       ctcttccgatctCACTCATATAGGTGGCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCTGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGc                                                                                                <  2:605024/132‑1 (MQ=255)                                                          cGGCAAGTTCACTCATATAGGTGGGGCTGGCGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCTGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGaaa                                                                                       <  1:75784/149‑1 (MQ=255)                                                          cGGCAACTTCACTCATATAGGTGGCGCTGGTGCCTTATTCTCCGCCAACAGATAATCCCTGAGATAAACGAGCGAGAAGCAATAATGCCTGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGaaa                                                                                       <  1:536782/149‑1 (MQ=255)                                                           ggCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCTGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATc                                                                                            <  2:450303/143‑1 (MQ=255)                                                           ggCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCTGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAac                                                                                      >  1:405549/1‑149 (MQ=255)                                                            gCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCCGCCAACAGACAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCTGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAcca                                                                                     >  1:542870/1‑149 (MQ=255)                                                             cAACTCCACTCATATAGGTGGCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCTGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAacac                                                                                    <  1:142989/149‑1 (MQ=255)                                                               aCTTCACTCATATAGGTGGGGTTGGGTCCAGATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCTGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACAcat                                                                                  <  1:671721/149‑1 (MQ=255)                                                                cTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAAGGCCTGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGaaa                                                                                       <  2:244347/143‑1 (MQ=255)                                                                 ttCACTCATATAGGCGGCGCTGGTGCCTGATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCTGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAac                                                                                      <  2:368117/143‑1 (MQ=255)                                                                   gaCTCATATGGGTGGCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCGGAAATAAACGGGCGAGAAGCAATAATGCCTGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACACATCATa                                                                              <  1:641632/148‑1 (MQ=255)                                                                    aCTCATATAGGTGGCCCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCTGAGCCCACGTACCTATAGTTTCATAACCTGCGAGGCGGGCGCTAACCCGCGCTCCGAAAcaca                                                                                   >  2:432293/1‑143 (MQ=255)                                                                       cATATAGGTGGCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCTGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACAcatca                                                                                <  2:472591/143‑1 (MQ=255)                                                                        atatAGGCGGCGCTGGCGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCTGAGCCCACGTACCCATAGTTTCATAACCTGGGAGGCAGGCGATAACTAGCGATCCGAAACACATCATacacac                                                                         <  1:216653/149‑1 (MQ=255)                                                                           taGGTGGCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCTGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACACATCATaca                                                                            <  2:209593/143‑1 (MQ=255)                                                                              gTGGCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCTGAGCCCACGTACCTATAGTTTCATAACCTGGGCGGCAGGCGATAACCAGCGATCCGAAACACATCATACACACCGATaa                                                                   >  1:161932/1‑149 (MQ=255)                                                                                 gcgcTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCTGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACACATCATACACACCGa                                                                      <  2:438162/143‑1 (MQ=255)                                                                                     tGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCTGAGCCCACGTACCTATAGTTTCATAACCTGGCAGGCAGGCGATAACCAGCGATCCGAAACACATCATACACACCGATGAc                                                                  >  2:606001/1‑143 (MQ=255)                                                                                        tGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCTGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACACATCATACACACCGATAACAGCATGGAt                                                         >  1:319949/1‑149 (MQ=255)                                                                                           cATATTCTCCGCCAACAGATAATGCCTGAAATAAACGAGCGAGAAGCAATAATGCCTGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGAGAACCAGCGATCCGAAACACATCATACACACCGATAACAGCATc                                                            >  2:483662/1‑143 (MQ=255)                                                                                              atTCTCCGCCAACAGATAATCCCTGAAATACCCGAGCGAGAAGTCATCATGCCTGAGCCCACGTACCTATAGTTTCACAACCTTGAAGGCAGGCGATAACCAGCGATCCGAAACACATCATACACACCGATAACAGCATCGATTTTTTg                                                   <  1:69174/149‑1 (MQ=255)                                                                                                  tcCGCCAACAGATAATCCCTGAAATAAACGAGCGGGAAGCAATAATGCCTGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACAAATCATACAACCCGATAACAGCATCGAttgtt                                                     >  2:284013/1‑143 (MQ=255)                                                                                                     gCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCTGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACACATCATACACACCGATAACAGCATCGATTTTTTGc                                                  >  2:96961/1‑143 (MQ=255)                                                                                                      ccAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCTGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGCTAACCAGCGACCCCAAACACATCATCCACACCGATAACAGCACCGATTTTTTGCg                                                 >  2:74646/1‑143 (MQ=255)                                                                                                          cAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCTGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACACATCATACACACCGATAACAGCATCGCTTTTTTGCGACCATGTTTa                                       >  1:718121/1‑149 (MQ=255)                                                                                                                  cccTGAAATAAACGAGCGAGAAGCAATAATGCCTGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACACATCATACACACCGATAACAGCATCGATTTTTTGCGACCATGTTTATCGGCTAt                               <  1:145012/149‑1 (MQ=255)                                                                                                                   ccTGAAATAAACGAGCGAGAAGCAATAATGCCTGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACACATCATACACACCGATAACAGCATCGATTTTTTGCGACCATGTTTATCGGCTATg                              >  1:274723/1‑149 (MQ=255)                                                                                                                      gAAATAAACGAGCGCGAAGCAATAATGCCTGAGCCCTCGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACACATCATACACACCGATAACAGCATCGATTTTTTGCGCCCATGTTTATCGGCTATGCgg                           <  1:560167/149‑1 (MQ=255)                                                                                                                         aTAAACGAGCGAGAAGCAATAATGCCTGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACACATCATACCCACCGATAACAGCATCGATTTTTTGCGACCATGTTTATCGGCTATGCGGCCa                        <  1:156706/149‑1 (MQ=255)                                                                                                                              cgagcgagAAGCAATAATGCCTGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACACATCATACACACCGATAACAGCATCGATTTTTTGCGACCATGTTTATCGGCTATGCGGCCAAATAg                   <  1:578320/149‑1 (MQ=255)                                                                                                                              cgagcgagAAGCAATAATGCCTGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACACATCATACACACCGATAACAGCATCGATTTTTTGCGACCATGTTTATCGGCTATGCGGCCAAATAg                   <  1:547372/149‑1 (MQ=255)                                                                                                                                agcgagAAGCAATCATGCCTGGGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACACATCATACACACCGATAACAGCATCGATTTTTTGCGACCATGTTTATCGGCTATGCGGCCaa                       <  2:618583/143‑1 (MQ=255)                                                                                                                                        gCAATAATGCCTGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACACATCATACACACCGATAACAGCATCGATTTTTTGCGACCATGTTTATCGGCTATGCGGCCAAATAGCCaa               >  2:289858/1‑143 (MQ=255)                                                                                                                                          aataatGCCTGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACACATCATACACACCGATAACGGCATCGATTTTTTGCGACCATGTTTATCGGCTATGCGGCCAACTACCCAAGCggccat       >  1:458553/1‑144 (MQ=255)                                                                                                                                               tGCCTGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACACATCATACACACCGATAACAGCATCGATATATTGAGAACATGTTTATCGGCTATGCGGCCAAATAGCCCACCGACTATTGAgc  >  1:696942/1‑149 (MQ=255)                                                                                                                                                   |                                                                                                                                                 GTCCGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCGGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACACATCATACACACCGATAACAGCATCGATTTTTTGCGACCATGTTTATCGGCTATGCGGCCAAATAGCCAACCGCCTATTGGGC  >  CP009273/2718589‑2718879

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑