Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	2,718,735	G→T	54.2%	P124Q (CCG→CAG)	kgtP ←	alpha‑ketoglutarate transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	2,718,735	0	G	T	54.2%	4.4 / 10.1	17	P124Q (CCG→CAG)	kgtP	alpha‑ketoglutarate transporter
Reads supporting (aligned to +/- strand):  ref base G (6/2);  new base T (5/4);  total (11/6)
Fisher's exact test for biased strand distribution p-value = 6.20e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCGGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACACATCATACACACCGATAACAGCATCGATTTTTTGCGACCATGTTTATCGGCTATGCGGCCAAATAGCCAACCGCCTATTGGG  >  CP009273/2718602‑2718878                                                                                                                                      |                                                                                                                                                cGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCCTCAACGGCAACTTCACCCATATAGGTGGCGCTGGTGCCATATTCCTCGCCAACAGATAATCCCTGCAATAAACGAGCGAGAAGCAATAATGCCTGAGCCCACGt                                                                                                                                       <  1:102319/144‑1 (MQ=255)          cTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTAGCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCTGAGCCCACGTACCTATAGTTTCAt                                                                                                                         >  2:246746/1‑149 (MQ=255)                    gCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTTCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCGGAGCCCACGTACATATAGTTTCATAACCt                                                                                                                    >  1:151862/1‑144 (MQ=255)                     catAAAAACCTTTCCCCCCTTCAACGGCAACTTCACTCATATAGTTGGCGCTGGTGCCATATTCTCCGCCAACATATAATCCCTGAAATAAACGAGCTAGAAGCAATAATGCCTGAGCCCACGTACCTATAGTTTCATAACCTg                                                                                                                   <  1:115395/142‑1 (MQ=255)                      gTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCGTAGTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCGGAGCCCACGTACCTATAGTTTCATAACCTGGGaggc                                                                                                             >  2:48254/1‑149 (MQ=255)                                ttGCGCCCTTCAACGGCAACTTCACTCATATAGGTGACGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCTGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCCGGCGATAAc                                                                                                   >  2:44096/1‑149 (MQ=255)                                       cTTCAACGGCAACTTCACTCATATAGGCGGCGCTGCTGCCATATCCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCGGAGCCCACGTACCTATAGTTTCCTAACCTGGGAGGCAGGCGATAACCa                                                                                                 <  1:94598/144‑1 (MQ=255)                                                  aCTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCGGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGCTCCGaaa                                                                                      >  1:64466/1‑144 (MQ=255)                                                                ggTGGCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCGGAGCACACGTAACTATAGTTTCATAACCTGGGAGGCCGGCGATAACCAGCGACCCGAAAAACATCATacacac                                                                        >  1:61308/1‑144 (MQ=255)                                                                    gaccgggTGCCATATTCTCCGCCAACAGATAATCCCGGAAATAACCGAGCGAGAAGCAATAATGCCTGAGCCCACGTACCCATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACACATCATACACACCGAt                                                                    <  1:216303/139‑1 (MQ=255)                                                                         ggTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCGGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGCAACACATCATACACACCGCTACCAg                                                               >  1:212844/1‑144 (MQ=255)                                                                         cgTGCCATATTCTCGGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCTGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGGTCCGAAACACATCATACACACCGATAACAg                                                               >  1:143793/2‑144 (MQ=255)                                                                                               gATAATCCCTGAAATAAACGAGCGCGCAGCAATAATGCCGGAGCCCACATACCTATAGATTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACACATCATACACACCGATAACAGCATCGATTTTTTGCGACCATGTTTATc                                    >  2:54404/1‑149 (MQ=255)                                                                                                           aaTCAACGAGCGAGAAGCAATAATGCCTGAGACCACGTACCTATAGTTTCATAACCTGGGAGGCAGGAGATAACAAGCGATCCGAAACACATCATACACCCCGCTACCAGCACAGGTTTTTTGCGACGAGCTTTAACGGCTCTg                             >  1:61938/1‑144 (MQ=255)                                                                                                                                aatGCCGGAGCCCACGTACCNANAGTNTCATACCCTGGGAGGCAGGCGATAACCAGCGATCCGAAACACATCATACACACCGATAACAGCATCGATTTTTTGCGATTATGTTTATCGGTTATGCGGCTAAATAGCCAACCGCCTATTgg   <  2:134592/149‑1 (MQ=255)                                                                                                                                 atGCCTGGGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACACTTCATAAACATCGATAAATGCATTGATTTTTTGTGTCTTTGTTTTTCGGCTATGCGGCCAAATAGCCAACCGCCTATTggg  <  2:52857/149‑1 (MQ=255)                                                                                                                                 atGCCTGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACACATCATACACACCGATAACAGCATCGATTTTTTGCGACCATGTTTATCGGCTATCCGGCCAAATAGCCAACCGCCTATTggg  >  2:82572/1‑149 (MQ=255)                                                                                                                                      |                                                                                                                                                CGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCGGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACACATCATACACACCGATAACAGCATCGATTTTTTGCGACCATGTTTATCGGCTATGCGGCCAAATAGCCAACCGCCTATTGGG  >  CP009273/2718602‑2718878

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑