| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 4,175,524 | G→T | 61.5% | V83F (GTC→TTC) | rpoC → | RNA polymerase, beta prime subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 4,175,524 | 0 | G | T | 61.5% | 11.7 / 12.9 | 23 | V83F (GTC→TTC) | rpoC | RNA polymerase, beta prime subunit |
| Reads supporting (aligned to +/- strand): ref base G (4/5); new base T (6/8); total (10/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.46e-01 | |||||||||||
TTCGGTGAAGTTAAAAAGCCGGAAACCATCAACTACCGTACGTTCAAACCAGAACGTGACGGCCTTTTCTGCGCCCGTATCTTTGGGCCGGTAAAAGATTACGAGTGCCTGTGCGGTAAGTACAAGCGCCTGAAACACCGTGGCGTCATCTGTGAGAAGTGCGGCGTTGAAGTGACCCAGACTAAAGTACGCCGTGAGCGTATGGGCCACATCGAACTGGCTTCCCCGACTGCGCACATCTGGTTCCTGAAATCGCTGCCGTCCCGTATCGGTCTG > CP009273/4175380‑4175655 | ttCGGTGAAGTTAAAAAGCCGGAAACCATCAACTACCGTACGTTCAAACCAGAACGTGACGGCCTTTTCTGCGCCCGTATCTTTGGGCCGGTAAAAGATTACGAGTGCCTGTGCGGTAAGTACAGGCGCCTGAAACACCGTGGCTTCAt < 2:202202/149‑1 (MQ=255) aaaGCCGGAAACCATCAACTACCGTACGTTCAAACCAGAACGTGACGGCCTTTTCTGCGCCCGTATCTTTGGGCCGGTAAAAGATTACGAGTGCCTGTGTGGTAAGTACAAGCGCCTGAAACACCGTGGCTTCATCTGTGAGAAGTgcg < 2:7640/149‑1 (MQ=255) ggAAACCATCAACTACCGTACGTTCAAACCAGAACGTGACGGCCTTTTCTGCGCCCTTATCTTTGGGCCGGTAAAAGATTACGAGTGCCTGTGCGGTAAGTACAAGCGCCTGAAACACCGTGGCTTCATCTGTGAGAAGGgcgg > 1:650/1‑144 (MQ=255) ccATCAACTACCGTACGTTCAAACCAGAACGTGACGGCCTTTTCTGCGCCCGTATCTTTGGGCCGGTAAAAGATTACGAGTGCCTGTGCGGTAAGTACAAGCGCCTGAAACACCGTGGCGTCATCTGTGAGAAGTGCGGCGTTg > 1:233195/1‑144 (MQ=255) cATCAACTACCGTACGTTCAAACCAGAACGTGACGGCCTTTTCTACGCCCGAAACTTGAAGCCGGGAAAAGATTACGAGTGCCTGTGCGGTAAGTACAAGCGCCTGAAACACCGTGGCTTCATCTGTGAGAAGTGCGGCGTTGAAGTGa > 2:136774/1‑149 (MQ=255) cTACCGTACGTTCAAACCAGAACGTGACGGCCTTTTCTGCGCCCGTATCTTTGGGCCGGTAAAAGATTACGAGTGCCTGTGCGGTAAGTATAAGCGCCTGAAACATCGTGGCTTCATCTGTGAGAAGTGCGGCGTTGAAGTGACCCAGa < 2:85008/149‑1 (MQ=255) ccGTACGTTCAAACCAGAACGTGACGGCCTTTTCTGCGCCCGTATCTTTGGGCCGGTAAAAGATTACGAGTGCCTGTGCGGTAAGTACAAGCGCCTGAAACACCGTGGCTTCATCTGTGAGAAGTGCGGCGTTGAAGTGACCCa > 1:103871/1‑144 (MQ=255) tCAAACCAGAACGTGACGGCCTTTTCTGCGCCCGTATCTTTGGGCCGGTAAAAGATTACGAGTGCCTGTGCGGTAAGTACAAGCGCCTGAAACACCGTGGCTTCATCTGTGAGAAGTGCGGCGTTGAAGTGACCCAGACTAAAg < 1:220207/144‑1 (MQ=255) aaCCAGAACGTGACGGCCTTTTCTGCGCCCGTATCTTTGGGCCGGTAAAAGATTACGAGTGCCTGTGCGGTAAGTACAAGCGCCTGAAACACCGTGGCGTCATCTGTGAGAAGTGCGGCGTTGAAGTGACCCAGACTAAAGTACGCCGt < 2:268200/149‑1 (MQ=255) gACGGCCTTTTCGGCGCCCGTGTCTTTGGGCCGGTAAAAGATTACGAGTGCCTGTGCGGTAAGTACAAGCGCCTGAAACACCGTGGCTTCATCTGTGAGAATTGCGGCGTTGAAGTGACCCAGACTAAAGTACGCCGTGAGCGTATggg < 2:27007/149‑1 (MQ=255) ccgcgcCCGTATCTTTGGGCCGGTAAAAGATTACGAGTGCCTGTGCGGTAAGTACAAGCGCCTGAAACACCGTGGCTTCATCTGTGAGAAGTGCGGCGTTGAAGTGACCCAGACTAAAGTACGCCGTGAGCGTATGGGCCACAt < 1:123589/142‑1 (MQ=255) cGTATCTTTGGGCCGGTAAAAGATTACGAGTGCCTGTGCGGTAAGTACAAGCGCCTGAAACACCGTGTCGTCATCTGTGAGAAGTGCGGCGTTGAAGTGACCCAGACTAAAGTACGCCGTCAGCGTATGGGCCACATCGAACTGGCTTc < 2:202677/149‑1 (MQ=255) aTCTTTGGGCCGGTAAAAGATTACGAGTGCCTGTGCGGTAAGTACAAGCGCCTGAAACACCGTGGCTTCATCTGTGAGAAGTGCGGCGTTGAAGTGTCCCAGTCTAAAGTACGCCGTGAGCGTATGGGCCACAGCGAACTGGCTTcccc < 2:801/149‑1 (MQ=255) gggCCGGTAAAAGATTACGAGTGCCTGTGCGGTAAGTACAAGCGCCTGAAACACCGTGGCTTCATCTGTGAGAAGTGCGGCGTTGAAGTGACCCAGACTAAAGTACGCCGTGAGCGTATGGGCCACATCGAACTGGCTTCCCCg < 1:162534/144‑1 (MQ=255) tAAAAGATTACGAGTGCCTGGGCGGTAAGTACAAGCGCCTGAAACACCGTGGCGTCATCTGTGAGAAGTGCGGCGTTGAAGTGACCCAGACTAAAGTACGCCGTGAGCGTATGGGCCACATCGAACTGGCTTCCCCGACTgcgc < 1:110783/144‑1 (MQ=255) aTTACGAGTGCCTGTGCGGTAAGTACAAGCGCCTGAAACACCGTGACGTCATCTGTGAGAAGTGCGGCGTTGAAGTAACCCAGACTAAAGTACGCCGTGAGCGTATGGGCCACATCGAACTGGCTTCCCCGACTGCGCACATCTGCTTc > 2:8998/1‑149 (MQ=255) aGTGCCTGTGCGGGTAGTACAAGCGCCTGAAACACCGTGGCGTCATCTGTGAGAAGTGCGGCGTTGAAGTGACCCAGACTAAAGTACGCCGTGAGCGTATGGGCCACATCGAACTGGCTTCCCCGACTGCGCACATCTGGTTCCTGaaa < 2:127903/149‑1 (MQ=255) gTGCCTGTGCGGTAAGTACAAGCGCCTGAAACACCGTGACTTCATCTGTGAGAAGTGCGGCATTGAAGTGACACAGACTAAAGTACGCCATGAGCGTATGGGCCACATCGAACTGGCTTCCCCGACTGCGCACATCTGGTTACTGAAAt > 2:119054/1‑149 (MQ=255) cTGTGCGGTAAGTACAAGCGCCTGAAACACAGTGGCTTCATCTGTGAGAAGTGCGGCGTTGAAGTGACGCAGACGAAAGTACGCCGTGAGCGTATGGGCCAAATCGACCTGGCTTCCACGACTGCGACAATCAGGTTGCTaaaa > 1:50633/1‑144 (MQ=255) tgtgCGGTAAGTACAAGCGCCTGAAACACCGTGGCGTCATAAGTGAGAAGTGAAACGTTAAAGTGAACCAGACTAAAGTACGCCGTGAGCGTATGGGCCACATCGAACTGGCTTCCCCGACTGCGCACATCTGGTTCCTGAAATCGCTg > 2:99689/1‑149 (MQ=255) gcgcCTGAAACACCGTGGCTTCCTCTGTGAGAAGTGCGGCGTTGAAGTGACCCAGACTAAAGTACGCCGTGAGCGTATGGGCCACATCGAACTGGCTTCCCCGACTGCGCACATCTGGTTCCTGAAATCGCTGCCGTCCCGTATCGGTc > 2:76243/1‑149 (MQ=255) gcCTGAAACACCGTGGCGTCATCTGTGAGAAGTGCGGCGTTGAAGTGACCCAGACTAAAGTACGCCGTGAGCGTATGGGCCACATCGAACTGGTTTCCCCGACTGCGTACATCTGGTTCCTGAAATCGCTGCCGTCCCGTATCGGTctg < 2:255232/149‑1 (MQ=255) gAAACACCGTGGCGTCATCTGTGAGAAGTGCGGCGTTGAAGTGACCCAGACTAAAGTACGCCGTGAGCGTATGGGCCACATCGAACTGGCTTCCCCGACTGCGCACATCTGGTTCCTGAAATCGCTGCCGTCCCGTATCGGTct > 1:113546/1‑144 (MQ=255) | TTCGGTGAAGTTAAAAAGCCGGAAACCATCAACTACCGTACGTTCAAACCAGAACGTGACGGCCTTTTCTGCGCCCGTATCTTTGGGCCGGTAAAAGATTACGAGTGCCTGTGCGGTAAGTACAAGCGCCTGAAACACCGTGGCGTCATCTGTGAGAAGTGCGGCGTTGAAGTGACCCAGACTAAAGTACGCCGTGAGCGTATGGGCCACATCGAACTGGCTTCCCCGACTGCGCACATCTGGTTCCTGAAATCGCTGCCGTCCCGTATCGGTCTG > CP009273/4175380‑4175655 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |