| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 3,708,519 | G→A | 19.1% | N412N (AAC→AAT) | bisC ← | biotin sulfoxide reductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 3,708,519 | 0 | G | A | 19.1% | 30.4 / 4.8 | 21 | N412N (AAC→AAT) | bisC | biotin sulfoxide reductase |
| Reads supporting (aligned to +/- strand): ref base G (9/8); new base A (2/2); total (11/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.64e-02 | |||||||||||
TTCAGAGATCACCACCAGCTCCGGTTTTTGCCAGGCACGGATCAGGCGATTGGTATCCTGATGATGAGTAAAGTTGGCACCGCCCGCCCACCAGATAAAACGAATATCCGGGAAATGTCGGTTCATACCGTTGTGTTGATATGCGCCACCAGGGTTTTCCAGTGCTTCAACAATGCGGGCAACAGGGATTTTATCCACCGCATCGCAGCCACCCGGCAAGCTGCCCTGCATGGAAGAGAGCACCGCAGAACGCCGCGTGGGGTTAC > CP009273/3708390‑3708655 | ttCAGAGATCACCGCCAGCTCCGGTTTTTGCCAGGCAAGAATCAAGCGAATGGTAACCTGATGATGAGTAAAGTTGGCACCGCCCGCCCACCAGATAAAACGAATATCCGGGCAATGTCAGTTCATACCGTTGTGGTGATATGCGccac > 2:3607/1‑149 (MQ=255) gagaTCACCACCAGCTCCGGTTTTTGCCAGGCACGGATCAGGCGATTGGTATCCTGATGATGAGTAAAGTTGGCACCGCCCGCCCACCAGATAAAACGAATATCCGGGTAATGTCGGTTCATACCGTTGTGTTGATATGCGCCACCAgg < 2:105869/149‑1 (MQ=255) aTCACCACCAGCTCCGGTTTTTGCCAGGCACGGATCAGGCGATTGATATCCTGATGATGAGAAAAGTTGGCACCGCCCGCCCACCAGATAAAACGAATATCCGGGAAATGTCGGTTCATACCGTAGCGTTGATATGCGCCCCCAggggt > 2:44291/1‑147 (MQ=255) caccccccggCTCCGGTGTTTGCGAGGCACGGAGCAGCTGATTGGTATCCTGATGATGAGTAAAGTTGGCACCGCCCGGCCACCAGATAAAACGAATATCCGGGAAATGTCGGTTCATACCGTTGTGTTGATATGCGCCACCAg < 1:146926/135‑1 (MQ=255) caccacCAGCTCCGGTTTTTGCCAGGCACGGATCAGGCGATTGGTATCCTGATGATGAGTAAAGTTGGCACCGCCCGCCCACCAGATAAAACGAATATCCGGGAAATGTCGGTTCATACCGTTGTGTTGATATGCGCCACCCgg > 1:166816/1‑144 (MQ=255) tGCCAGGCACGGATCAGGCGATTGGTATCCTGATGATGAGTAAAGTTGGCACCGCCCGCCCACCAGATAAAACGAATATCCGGGAAATGTCGGTTCATACCATTGTGTTGATATGCGCCACCAGGGTTTTCCAGTGCTTCAACAATGCg > 2:278062/1‑149 (MQ=255) tGCCAGGCACGGATCAGGCGATTGGTATCCTGATGATGAGTAAAGTTGGCACCGCCCGCCCACCAGATAAAACGAATATCCGGGAAATGTCGGTTCATACCATTGTGTTGATATGCGCCACCAGGGTTTTCCAGTGCTTCAACAATGCg > 2:278024/1‑149 (MQ=255) ggCGATTGGTATCCTGATGATGAGTAAAGTTGGCACCGCCCGCCCACCAGATAAAACGAATATCCGGGAAATGTCGGTTCATACCGTTGTGTTGATATGCGCCACCAGGGTTTTCCAGTGCTTCAACAATGCGGGCAACAGGGATTTTa < 2:136098/149‑1 (MQ=255) cGATTGGTATCCTGATGATGAGTAAAGTTGGCACCGCCCGCCCACCAGATAAAACGAATATCCGGGAAATGTCGGTTCATACCGTTGTGTTGATATGCGCCACCAGGGTTTTCCAGTGCTTCAACAATGCGGGCAACAGGGATTTTATc < 2:184379/149‑1 (MQ=255) gATTGGTATCCTGATGATGAGTAACGTTGGCACCACCCGCCCACCAGATAAAACAAATAACCGGGAAATGTCGATTCATACCGTTGTGTTGATATGCGCCACCAGGGTTTTCCAGTGCTTCAACAATGCGGGAAACAGGGATTTTGTcg > 2:78993/1‑148 (MQ=255) ggTAGCCTGAGGATGAGTAAAGTTGGCACCTCCCGCCCACCAGATAAAACGAATATCCGGGAAATGTCGTTTCATACCGTTGTGTTGATATGCGCCACCAGGGTTTTCCAGTGCTTCAACAATGCGGGCAACAGGGATTTTATCCACCg < 2:116085/149‑1 (MQ=255) tCCTGATGATGAGTAAAGTTGGCACCGCCCGCCCACCAGATAAAACGAATATCCGGGAAATGTCGGTTCATACCGTTGTGTTGATATGCGCCACCAGGGTTTTCCAGTGCTTCAACAATGCGGGCAACAGGGATTTTATCCACCGCATc > 2:224368/1‑149 (MQ=255) tgaGTAAAGTTGGCACCGCCCGCCCACCAGATAAAACGAATATCCAGGAAAAGTCGGAACATACCGTAGAGTTGATATGCGCCACCAGGGTTTTCCAGTGCTTCAACAATGCGGGCAACAGGGATTTTATCCACCGCATCGCAGCCAcc > 2:148385/1‑149 (MQ=255) gTAAAGTTGGCACCGCCCGCCCACCAGATAAAACGAATATCCGGGGAATGTCGGGTCATACCGTTGTGTTGATATTCGCCACCAGGGTTGTACAGTGCTTTAACAATGCGGGCAACAGGGAGTTTATGCACCGCATCGCAGCCa > 1:184856/1‑144 (MQ=255) taGTTGGCACCGCCCGCCCACCAGATAAAACGAATATCCGGGAAATGTCGGTTCATACCATTGTGTTGATATGCGCCACCAGGGTTTTCCAGTGCTTCAACAATGCGGGCAACAGGGATTTTATCCACCGCATCGCAGCCAccc < 1:254572/143‑1 (MQ=255) aaGTTGGCACCGCCCGCCCACCAGATAAAACGAATATCCGGGAAATGTCGGTTCATACCATTGTGTTGATATGCGCCACCAGGGTTTTCCAGTGCTTCAACAATGCGGGCAACAGGGATTTTATCCACCGCATCGCAGCCAccc < 1:254609/144‑1 (MQ=255) aGTTGGCACCGCGCGCCGATCAGATAAAACGAATATCCGGGAAATGTCGGTTCATACCGTTGTGTTGATATGCGCCACCAGGGTTTTCCAGTGCTTCAACAATGCGGGCAACAGGGATTTTATTCACCGCATGGCAGTCACCCg < 1:82464/144‑1 (MQ=255) accaGATAAAACGAATATCCGGGAAATGTCGGTTCATACCGTAGTATTGATATGCGCCACCAGGGTTTTCCAGTGCTTCAACAATGCGGGCAACAGGGATTTTATCCACCGCATCGCAGCCACCCGGCAAGCTGCCCTGCATGGAagag > 2:140731/1‑149 (MQ=255) aaaaCGAATATCCTGAAGATGAGTGTTCGTACCGTTGTGGCGATATGCTTCACCAGGGTTTTCCAGTGCTTCAACAATGCGGGCAACAGGGATTTTATCCACCGCATCGCAGCCACCCGGCAAGCTGCCCTGCATGGAagagag < 1:92485/144‑1 (MQ=255) ccGGGAAATGTCGGTTCATACCGTTGTGTTGATATGCGCCACCAGGGTTTTCCAGTGCTTCAACAATGCGGGCAACAGGGATTTTATCCACCGCATCGCAGCCACCCGGCAAGCTGCCCTGCATGGAAGAGAGCACCGCAGAAcgccgc > 2:148762/1‑149 (MQ=255) tCGGTTCATACCGTTGTGTTGATATGCTCCACCAGGGTTTTCCAGTGCTTCAACAATGCGGGCAACAGGGATTTTATCCACCGCATCGCAGCCACCCGGTAAGGTGTCCTGCATGGAAGAGAGCACCGCAGAACGCCGCGTGGGGTTac < 2:46295/149‑1 (MQ=255) | TTCAGAGATCACCACCAGCTCCGGTTTTTGCCAGGCACGGATCAGGCGATTGGTATCCTGATGATGAGTAAAGTTGGCACCGCCCGCCCACCAGATAAAACGAATATCCGGGAAATGTCGGTTCATACCGTTGTGTTGATATGCGCCACCAGGGTTTTCCAGTGCTTCAACAATGCGGGCAACAGGGATTTTATCCACCGCATCGCAGCCACCCGGCAAGCTGCCCTGCATGGAAGAGAGCACCGCAGAACGCCGCGTGGGGTTAC > CP009273/3708390‑3708655 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |