| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 3,350,594 | G→A | 19.1% | T837T (ACG→ACA) | gltB → | glutamate synthase, large subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 3,350,594 | 0 | G | A | 19.1% | 36.0 / 4.6 | 21 | T837T (ACG→ACA) | gltB | glutamate synthase, large subunit |
| Reads supporting (aligned to +/- strand): ref base G (6/11); new base A (2/2); total (8/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.18e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.38e-01 | |||||||||||
CCACGCCTACAACCCGGACGTGGTGCGCACGCTGCAACAAGCGGTACAAAGCGGCGAGTACAGCGACTATCAGGAATACGCGAAGCTGGTTAATGAGCGTCCGGCAACCACGCTGCGCGATCTGCTGGCAATTACGCCGGGTGAAAACGCGGTCAACATTGCTGATGTTGAACCGGCAAGCGAACTGTTTAAACGCTTTGATACCGCCGCGATGTCTATCGGCGCGTTAAGCCCGGAAGCCCACGAGGCGCTGGCGGAAGCGATGAACAGCATCGGCGGTAATT > CP009273/3350459‑3350742 | ccACGCCTACAACCCGGCCGTGGGACGCACGCTGCAACAAGCGGTACACAGCGGCAAGAACAGCGACTATCAGGAATACGCGAAGCGGGTTAATCGGCGTCCGGCAACCACCCTGCGCCACCTGCTGGCAATTACGCCGGGTGa > 1:110570/1‑144 (MQ=255)ccACGCCTACAACCCGGACGTGGTGCGCACGCTGCAACAAACGGAACAAAGCAGCGAGAACAGCGACTATCAGGAATACGCGAAGCTGGTTAATGAGCGTCCGGCAACCACGCTGCGCGATCTGCTGGCAATTACACCGGGTGAAAAcg > 2:20197/1‑149 (MQ=255) cAACCCGGACGTGGTGCGCACGCTGCAACAAGCGGTACAAAGCGGCGAGTACAGCGACTATCAGGAATACGCGAAGCTGGTTAATGAGCGTCCGGCAACCACGCTGCGCGATCTGCCGGCAATTACGCCGGGTGAAAACGCGGt < 1:207406/144‑1 (MQ=255) ccGGACGTGGTGCGCACGCTGCAACAAGCGGTACAAAGCGGCGAGTACAGCGACTATCAGGAATACGCGAAGCTGGTTAATGAGCGTCCGGCAACCACGCTGCGCGATCTGCTGGCAATTACACCGGGTGAAAACGCGGTCAACATTGc > 2:278952/1‑149 (MQ=255) ggACGTGGTGCGCACGCTGCAACAAGCGGTACAAAGCGGCGAGTACAGCGACTATCAGGAATACGCGAAGCTGGTTAATGAGCGTCCGGCAACCACGCTGCGCGATCTGCTGGCAATTACGCCGGGTGAAAACGCGGTCAACAt < 1:172202/144‑1 (MQ=255) gcgcACGCTGCAACAAGCGGTACAAAGCGGCGAGTACAGCGACTATCAGGAATACGCGAAGCTGGTTAATGAGCGTCCGGCAACCACGCTGCGCGATCTGCTGGCAATCCCGCCGTGTGAAAACGCGGTCAACATTGCTGATGt < 1:106571/144‑1 (MQ=255) acaaGCGGTACAAAGCGGCGAGTACAGCGACTATCAGGAATACGCGAAGCTGGTTAATGAGCGTCCGGCAACCACGCTGCGCGATCTGCTGGCAATTACGCCGGGTGAAAACGCGGTCAACATTGCTGATGTTGAACCGGCAAg < 1:251179/144‑1 (MQ=255) aCAGCGACTATCAGGAATACGCGAAGCTGGTTAATGAGCGTCCGGCAACCACGCTGCGCGATCTGCTGGCAATTACGCCGGGTGAAAACGCGGTCAACATTGCTGATGTTGAACCGGCAAGTGAACTGTTTAAACGCTTTGATAccgcc > 2:186283/1‑149 (MQ=255) ccAGGAATACGCGAAGCTGGTTAATGAGCGTCCGGCAACCACGCTGCGCGATCTGCTGGCAATTACGCCGGGTGAAAACGCGGTCAACATTGCTGATGTTGAATTGGTAATTGAACTGTTTAAACGCTTTGATACCGCCGCGATGTCTa < 2:83881/148‑1 (MQ=255) aTACGCGAAGCTGGTTAATGAGCGTCCGGCAACCACGCTGCGCGATCTGCTGGCAATTACGCCGGGTGAAAACGCGGTCAACATTGCTGATGTTGAACCGGCAAGCGAACTGTTTAAACGCTTTGATACCGCCGCGATGTCTAt > 1:113313/1‑144 (MQ=255) gcgAAGCTGGTTAATTAGCGTCCGGCAACCACGCTGCGCGATCTGCTGGCAATTACACCGGGTGAAAACGCGGTCAACATTGCTGATGTTGAACCGGCAAGCGAACTGTTTAAACGCTTTGATACCGCCGCGATGTCTATCGgc < 1:255463/144‑1 (MQ=255) cTGGTTAATGAGCGTCCGGCAACCACGCTGCGCGGTCTGCTGGCAATTACGCCGGGTGAAAACGCGGTCAACATTGCTGATGTTGAACCGGCAAGCGAACTGTTTAAACGCTTTGATACCGCCGCGATGTCTATCGGCGCGTTAAGccc > 2:50239/1‑149 (MQ=255) tAATGAGCGCCCGGCAACCACGCCGCGCGATCTGCTGGCAATTACACCGGGTGAAAACGCGGTCAACATTGCTGATGTTGAACCGGCAAGCGAACTGTTTAAACGCTTTGATACCGCCGCGATGTCTATCGGCGCGTTAAGccc < 1:18555/144‑1 (MQ=255) ggAGCCTCCGGCAACCCCGCTGCGCGATCTGCTGGCAATTACGCCGGGTGAAAACGCGGTCAACATTTCTGATGTTGAACCGGCAAGCGAACTGTTTAAACGCTTTGATACCGCCGCGATGTCTATCGGCGCGTTAAGCCCGGa < 1:5277/143‑1 (MQ=255) gCTGCGCGATCTGCTGGCAATTACGCCGGGTGAAAACGCGGTTAACATTGCTGATGTTGAACCGGCAAGCGAACTGTTTAAACGCTTTGATACCGCTGCGATGTTTATCGGTGCGTTAAGCCCGGAAGCCCACGAGGCGCTGGCGGAAg < 2:104057/149‑1 (MQ=255) cgATCTGCTGGCAATTACGCCGGGTGAAAACGCGGTCAACATTGCTGATGTTGAACCGGCAAGCGAACTGTTTAAACGCTTTGATACCGCCGCGATGTCTATCGGCGCGTTAAGCCCGGAAGCCCACGAGGCGCTGGCGGAAGc < 1:46117/144‑1 (MQ=255) gATCTGCTGGCAATTACGCCGGGTGCAAACGCGGTCAACATTGCTGATGTTGAACCGGCAAGCGAACTGTTTAAACGCTTTGATACCGCCGCGATGTCTATTGACGCGTTAATCCCGGAAGCCGACGAGGCGCTGGCGGAAGCGCTGaa > 2:57931/1‑149 (MQ=255) gATCTGCTGGCAATTACGCCGGGTGAAAACGCGGTCAACATTGCTGATGTTGAACCGGCAAGCGAACTGTTTAAACGCTTTGATACCGCCGCGATGTCTATCGGCGCGTTAAGCCCGGAAGCCCACGAGGCGCTGGCGGAAGCg < 1:235214/144‑1 (MQ=255) gATCGGCTGGCAATTACGCCGGGTGAAAACGCGGTCAACATTGCTGATGTTGAACCGGCAAGCGAACTGTTTAAACGCTTTGATACCGCCGCGATGTCTTTCGGGGCGTTAAGCCCGGAAGCCCACGAGGCGCTGGCGGAAGCGATGaa < 2:183989/149‑1 (MQ=255) ttACGCCGGGTGAAGACGCGGTCAACATTGCTGATGTTGAACCGGCAAGCGAACTGTTTAGCTGCTCTGATACCGGCGCGATGTCTATCGGCGCGTTAAGCCCGGAAGCCGACGAGGCGCTGGCGGAAGCGATGAACAGCATCGGCGGt < 2:123332/149‑1 (MQ=255) tACGCCGGGTGAAAACGCGGTCAACATTGCTGATGTTGAACCGACAAACGAAATATTTAAACGCTTTGATACCGCCGCGATGTCTATCGGCGCGTTAAGCCCGGAAGCCCACGAGGCGCTGGCGGAAGCGATGAACAGCATCGGCGGTa > 2:259629/1‑149 (MQ=255) gCCGGGTGAAAACGCGGTCAACATTGCTGATGTTGAACCGGCAAGCGAACTGTTTAAACGCTTTGATACCGCCGCGATGTCTATCGGCGCGTTAAGCCCGGAAGCCCACGAGGCGCTGGCGGAAGCGATGAACAGCATCGGCGGTAAtt > 2:135156/1‑149 (MQ=255) | CCACGCCTACAACCCGGACGTGGTGCGCACGCTGCAACAAGCGGTACAAAGCGGCGAGTACAGCGACTATCAGGAATACGCGAAGCTGGTTAATGAGCGTCCGGCAACCACGCTGCGCGATCTGCTGGCAATTACGCCGGGTGAAAACGCGGTCAACATTGCTGATGTTGAACCGGCAAGCGAACTGTTTAAACGCTTTGATACCGCCGCGATGTCTATCGGCGCGTTAAGCCCGGAAGCCCACGAGGCGCTGGCGGAAGCGATGAACAGCATCGGCGGTAATT > CP009273/3350459‑3350742 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |