| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 2,796,564 | G→A | 19.1% | V620I (GTT→ATT) | nrdE → | ribonucleoside‑diphosphate reductase 2, alpha subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 2,796,564 | 0 | G | A | 19.1% | 33.1 / 3.8 | 21 | V620I (GTT→ATT) | nrdE | ribonucleoside‑diphosphate reductase 2, alpha subunit |
| Reads supporting (aligned to +/- strand): ref base G (9/8); new base A (2/2); total (11/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.03e-01 | |||||||||||
CGGCATATACAACCAGAATCTTCAGGCGGTGCCGCCAACCGGTTCTATCTCTTATATCAACCATGCTACGTCGAGTATTCATCCGATTGTGGCGAAAGTAGAGATACGCAAAGAGGGCAAAACAGGACGCGTTTACTACCCTGCCCCGTTTATGACTAACGAGAATCTGGCGCTGTATCAGGACGCTTACGAAATTGGCGCAGAAAAGATCATCGACACCTACGCGGAAGCGACTCGCCATGTCGATCAGGGGCTGTCGCTGACGCTTTT > CP009273/2796434‑2796703 | cagCATATACAACCAGAATCTTCAGGCGGTGCCGCCAACCGGTTCTATCTCTTATATCAACCATGCTACGTCGAGTATTCATCCGATTGTGGCGAAAGTAGAGATACGCAAAGAGGGCAAAACAGGACGCGTTTACTACCCTGc > 1:179201/3‑144 (MQ=255) taCAACCAGAATCTTCAGGCGGTGCCCCAATGTGAACCCAACCCTTATATCAACCATGCTACGTCGAGTATTCATCCGATTGTGGCGAAAGTAGAGATACGCAAAGAGGGCCAAACAGGCCGCGTTTACTACCCTGCCCCGTTTATGAc > 2:61362/1‑149 (MQ=255) cAACCAGAATCTTCAGGCGGTGCCGCCAACCGGTTCTATCTCTTATATCAACCATGCTACGTCGAGTCTTCATCCGATTGTGGCGAAAGTAGAGATACGCAAAGAGGGCAAAACAGGACGCGTTTACTACCCTGCCCCGTTTAt > 1:227035/1‑144 (MQ=255) gcAGAATCTTCAGGCGGTGCCGCCAACCGGTTCTATCTCTTATATCAACCATGCTACGTCGAGTATTCATCCGATTGTGGCGAAAGTAGAGATACGCAAAGATGGTAAAACAGGACGCGTTTACTACCCTGCCCCGTTTATGACTAACg < 2:11869/148‑1 (MQ=255) tCTTCAGGCGGTGCCGCCAACCGGTTCTATCTCTTATATCAACCATACTACGTCGAGTATTCATCCAATTGTGGCGAAAGTAGAGATACGCAAAGAGGGCAAAACAGGACGCGTTTACTACCCTGCCCCGTTTATGACTAACGAGAATc > 2:246173/1‑149 (MQ=255) cgccAACCGGTTCTATCTCTTATATCAACCATGCTACGTCGAGTATTCATCCGATTGTGGCGAAAGTAGAGATACGCAAAGAGGGCAAAACAGGACGCGTTTACTACCCTGCCCCGTTTATGACTAACGAGCATCTGGGGCTGt > 1:67092/1‑144 (MQ=255) aaCCGGTTCTATCTCTTATATCAACCATGCTACGTCGAGTATTCATCCGATTGTGGCGAAAGTAGAGATACGCAAAGAGGGCAAAACATGACGCGTTTACTACCCTGCCCCGTTTATGACTAACGAGAATCTGGCGCTGTATCAGGACg < 2:258162/149‑1 (MQ=255) aaCCGGTTCTATCTCTTATATCAACCATGCTACGTCGAGTATTCATCCGATTGTGGCGAAAGTAGAGATACGCAAAGAGGGCAAAACAGGACGCGTTTACTACCCTGCCCCGTTTATGACTAACGAGAATCTGGCGCTGTATCAGGACg > 2:179011/1‑149 (MQ=255) tATCTCTGATAGCAACCCTGCTACTTCGAGTATTCATCCGATTGTGGCGAAAGTAGAGATACGCAAAGAGGGCAAAACAGGACGCGTTTACTACCCTGCCCCGTTTATGACTAACGAGAATCTGGCGCTGTATCAGGACGCTTa < 1:56465/144‑1 (MQ=255) ctTATATCAACCATGCTACGTCGAGTATTCATCCGATTGTGGCGAAAGTAGAGATACGCAAAGAGGGCAAAACAGGACGCGTTTACTACCCTGCCCCGTTTATGACTAACGAGAATCTGGCGCTGTATCAGGACGCTTACGAAATTGgc > 2:262153/1‑149 (MQ=255) atatCAACCATGCTACGTCGAGTATTCATCCGATTGTGGCGAAAGAAGAGATACGCAAAGAGGGCAAAACAGGACGCGTTTACTACCCTGCCCCGTTTATGACTAACGAGAATCTGGCGCTGTATCAGGACGCTTACGAAATTGGCGCa > 2:123798/1‑149 (MQ=255) cTACCTCCACTATTCATCCGATTGTGGCAAAAGTAGAGATACGCAAAGAGGGCAAAACAGGACGCGTTTACTACCCTGCCCCGTTTATGACTAACGAGAATCTGTCGCTGTATCAGGACGCTCACGAAATTGGCGCAGAAAAGatcatc < 2:179440/149‑1 (MQ=255) cGTCGAGTATTCATCCGATTTTGGCGAAAGTAGAGATACGCAAAGAGGGCAAAACAGGACGCGTTTACTACCCTGCCCCGTTTATGACTAATGAGAATCTGGTGCTGTATCAGGACGCTTACGAAATTGGCGCAGAAAAGATCATCGac < 2:119361/149‑1 (MQ=255) cGATTGTGGCGAAAGTAGAGATACGCAAAGAGGGCAAAACAGGACGCATTTACTACCCTGCCCCGTTTATGACTAACGAGAATCTGGCGCTGTATCACGAAGCCATCGAAATTGGCGCAGAAAAGATCATCGACACCTACGCGGAAGCg > 2:195102/1‑149 (MQ=255) cGATTGTGGCGAAAGTAGAGATACGCAAAGAGGGCAAAACAGGACGCATTAACTACCCTGCCCCGTTTATGACTAACGAGAATCTGGCGCTGTATCAGGCAGCAAACGAAATTGACGCCGAAAAGATCATCGACACCTACGCGGAAGCg > 2:195161/1‑149 (MQ=255) gAAAGTAGAGATACGCAAAGAGGGCAAAACAGGACGCGTTTACTACCCTGCCCCGTTTATGACTAACGAGAATCTGGCGCTGTATCAGGACGCTTACGAAATTGGCGCAGAAAAGATCATCGACACCTACGCGGAAGCGACTCGCCATg > 2:213210/1‑149 (MQ=255) agGGCAAAACAGGACGCGTTTACTACCCTGCCCCGTTTATGACTAACGAGAATCTGGCGCTGTATCAGGACGCTTACGAAATTGGCGCAGAAAAGATCATCGACACCTACGCGGAAGCGACTCGCCATGTCGATCAGGGGCTGTCGCTg < 2:62061/149‑1 (MQ=255) aaaCAGGACGCGTTTACTACCCTGCCCCGTTTATGACTAACGAGAATCTGGCGCTGTATCAGGACGCTTACGAAATTGGCGCAGAAAAGATTATCGACACCTACGCGGAAGCGACCCGCCATGTCGATCAGGGGCTGTCGTTGACGCtt < 2:197441/149‑1 (MQ=255) cAGGACGCGTTTACTACCCTGCCCCGTTTATGACTAACGAGAATCTGGCGCTGTATCAGGACGCTTACGAAATTGGCGCAGAAAAGATCATCGACACCTACGCGGAAGCGACTCGCCATGTCGATCAGGGGCTGTCGCTGACGc < 1:161832/144‑1 (MQ=255) aCGCATTTACTATCCTGCCCCGTTTATGACTAACGAGAATCTGGCGCTGTATCAGGACGCTTACGAAATTGGCGCAGAAAAGATCATCGACACCTACGCGGAAGCGACTCGCCATGTCGATCAGGGGCTGTCGCTGACGCtttt < 1:176968/144‑1 (MQ=255) aCGCATTTACTACCCTGCCCCGTTTATGACTAACGAGAATCTGGCGCTGTATCAGGACGCTTACGAAATTGGCGCAGAAAAGATCATCGACACCTACGCGGAAGCGACTCGCCATGTCGATCAGGGGCTGTCGCTGACGCtttt < 1:176910/144‑1 (MQ=255) | CGGCATATACAACCAGAATCTTCAGGCGGTGCCGCCAACCGGTTCTATCTCTTATATCAACCATGCTACGTCGAGTATTCATCCGATTGTGGCGAAAGTAGAGATACGCAAAGAGGGCAAAACAGGACGCGTTTACTACCCTGCCCCGTTTATGACTAACGAGAATCTGGCGCTGTATCAGGACGCTTACGAAATTGGCGCAGAAAAGATCATCGACACCTACGCGGAAGCGACTCGCCATGTCGATCAGGGGCTGTCGCTGACGCTTTT > CP009273/2796434‑2796703 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |