Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 352,394 A→G 22.4% E171E (GAA→GAG)  codA → cytosine/isoguanine deaminase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273352,3940AG22.4% 23.4 / 2.0 18E171E (GAA→GAG) codAcytosine/isoguanine deaminase
Reads supporting (aligned to +/- strand):  ref base A (4/9);  new base G (2/2);  total (6/12)
Fisher's exact test for biased strand distribution p-value = 5.84e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01

CATGTCGATGTTTCGGATGCAACGCTAACTGCGCTGAAAGCAATGCTGGAAGTGAAGCAGGAAGTCGCGCCGTGGATTGATCTGCAAATCGTCGCCTTCCCTCAGGAAGGGATTTTGTCGTATCCCAACGGTGAAGCGTTGCTGGAAGAGGCGTTACGCTTAGGGGCAGATGTAGTGGGGGCGATTCCGCATTTTGAATTTACCCGTGAATACGGCGTGGAGTCGCTGCATAAAACCTTCGCCCTGGCGCAAAAATACGACCG  >  CP009273/352248‑352510
                                                                                                                                                  |                                                                                                                    
cATGTCGATGTTTCGGATGCAACGCTAACTGCGCTGAAAGCAATGCTGGAAGTGAAGCAGGAAGTCGCGCCGTGGATTGATCTGCAAATCGTCGCCTTCCCTTAGGAAGGGATTTTGTCGTATCCCAACGGTGAAGCGTTGCTGGAaga                                                                                                                    <  2:214028/149‑1 (MQ=255)
    tCGATGTTTCGGATGCAACGCTAACCGCGCTGAAAGCAATGCTGGAAGTGAAGCAGGAAGTCGCGCCGTGGATTGATCTGCAAATCGTCGCCTTCCCTCAGGAAGGGATTTTGTCGTATCCCAACGGTGAAGCGTTGCTGGAag                                                                                                                     <  1:96251/144‑1 (MQ=255)
          tttCGGATGCAACGCTAACTGCGCTGAAAGCAATGCTGGAAGTGAAGCAGGAAGTCGCGCCGTGGATTGATCTGCAAATCGTCGCCTTCCCTCAGGAAGGGATTTTGTCGTATCCCAACGGTGAAGCGTTGCTGGAAGAGGCGt                                                                                                               <  1:160224/144‑1 (MQ=255)
              ggATGCAACGCTAACTGCGCTGAAAGCAATGCTGGAAGTGAAGCAGGAAGTCGCGCCGTGGATTGATCTGCAAATCGTCGCCTTCCCTCAGGAAGGGATTTTGTCGTATCCCAACGGTGAAGCGTTGCTGGAAGAGGCGTTACg                                                                                                           >  1:231529/1‑144 (MQ=255)
                aTGCAACGCTAACTGCGCTGAAAGCAATGCTGGAAGTGAAGCAGGAAGTCGCGCCGTGGATTGATCTGCAAATCGTCGCCTTCCCTCAGGAAGGGATTTTGTCGTATCCCAACGGTGAAGCGTTGCTGGAGGAGGCGTTACGCTTAggg                                                                                                    >  2:42744/1‑149 (MQ=255)
                aTGCAACGCTAACTGCGCTGAAAGCAATGCTGGAAGTGAAGCAGGAAGTCGCGCCGTGGATTGATCTGCAAATCGTCGCCTTCCCTCAGGAAGGGATTTTGTCGTATCCCAACGGTGAAGCGTTGCTGGAGGAGGCGTTACGCTTAggg                                                                                                    >  2:179735/1‑149 (MQ=255)
                                gcggAAAGCAATGCTGGAAGTGAAGCACGAAGTCGCGCCGTGGATTGATCTGCAAATCGTCGCCTTCCCTCAGGAAGGGATTTTGTCGTATCCCAACGGTGAAGCGTTGCTGGAAGAGGCGTTACGCTTAGGGGCAGATGTAGTggggg                                                                                    <  2:186354/146‑1 (MQ=255)
                                              ttgAAGTGAAGCAGGAAGTCGCGCCGTGGATGGATCGGCAAGACGTTACCTTCCCTCAGGAAGGGATTTTGTCGTATCCCAACGGTGAAGCGTTGCTGGAAGAGGCGTTACGCTTAGGGGCAGATGTAGTGGGGGCGATTCCGCAtttt                                                                      <  2:57951/147‑1 (MQ=255)
                                                 aaGTGAAGCAGGAAGTCGCGCCGTGGATTGCTCTGCCATCCTTTGCCTTCCCTTAGGAAGGGATTTTGTCGTATCCCAACGGTTAAGCTTTGCTGGAAGAGGCGTTACGCTTAGGGGTAGATGTAGTGGGGGCGATTCCGCATTTTGaa                                                                   <  2:45590/149‑1 (MQ=255)
                                                       aGCAGGAAGTCGCGCCGTGGATTGATCTGCAAATCGTCGCCTTCCCTCAGGAAGGGATTTTGTCGTATCCCAACGGTGAAGCGTTGCTGGAAGAGGCGTTACGCTTAGGGGCAGAGGTAGTGGGGGCGATTCCGCATTATGaag                                                                  >  1:98120/1‑143 (MQ=255)
                                                          aGGAAGTCGCGCCGTGGATTGATCTGCAAATCGTCGCCTTCCCTCAGGAAGGGATTTTGTCGTATCCCAACGGTGAAGCGTTGCTGGAAGAGGCGTTATGCTTAGGGGCAGATGTAGTGGGGGCGATTCCGCATTTTGAATTTACCCGt                                                          <  2:216003/149‑1 (MQ=255)
                                                                        tGGATTGATCTGCAAATCGTCGCCTTCCCTCAGGAAGGGATTTTGTCGTATCCCAACGGTGAAGCGTTGCTGGAAGAGGCGTTACGCTTAGGGGTAGATGTAGTGGTGGCGATTTCGCATTTTGAATTTACCCGGGAATACGGCGTGGa                                            <  2:17994/149‑1 (MQ=255)
                                                                              gcttggCAAATCGTCGGGTTCCCTCAGGAAAGGATTTTGTCGTATCCCACCGGTGAAGCGTTGCTGGAAGAGGCGTTACGCTTAGGGGCAGATGTAGTGGGGGCGATTCCGCATTTTGAATTTACCCGTGAATACGGCGTGGAg                                           <  1:42723/139‑1 (MQ=255)
                                                                               aTCTGCAAATCGTCGCCTCCCCTCAGGAAGGAATTTTGTCGTATCCCAACGGTGAAGCGTTGCTGGAAGAGGCGTTACTTTTAGGGGTAGTTTTATTGGTTTCTATTCCGCATTTTGAATTTACCCGTGAATACGGCGTGGAGTCGCTg                                     <  2:95901/149‑1 (MQ=255)
                                                                                               cTTCCCTCAGGAAGGGATTTTGTCGTATCCCAACGATGAAGCATTACTGAAAGAGGCGATACGCTTAGGGGCAGATGTAGTGGGGGCGATTCCGCATTTTGAATTTACCCGTGAATACGGCGTGGAGTCGCTGCATAAAACCTTCGccc                     >  2:17185/1‑149 (MQ=255)
                                                                                                        ggAAGGGATTTTGTCGTATCCCAACGGTGAAGCGTTGCTGGACGAGGCGTTACGCTTAGGGGCAGATGTAGTGGGGGCGATTCCGCATTTTGAATTTACCCGTGAATACGGCGTGTGTTCGCTGCATAAGACCTTCGCCCTgga                 <  1:144076/144‑2 (MQ=255)
                                                                                                         gAAGGTATTTTGTCGTATCCCAGCGGTGAAGCGTTGCTGGAAGAGGCGGTACGCTTAGGGGCAGATGTAGTGGGGGCGATTCCGCATTTTGAATTTACCCGTGAATACGGCGTGGAGTCGCTGCATAAAACCTTCGCCCTGgcg                <  1:152136/144‑1 (MQ=255)
                                                                                                                     tCGTATCCCAACGGTGAAGCGTTGCTGGAGGAGGCGTTACGCTTAGGGGCAGATGTAGTGGGGGCGATTCCGCATTTTGAATTTACCCGTGAATACGGCGTGGAGTCGCTGCATAAAACCTTCGCCCTGGCGCAAAAATACGAc    <  1:162523/144‑1 (MQ=255)
                                                                                                                     tCGGATCCCCACGGTGAAGCGTTGCTGGAGGAGGGGTTACGCTTAGGGGCAGATGTAGTGGGGGCGATTCCGCATTTTGAATTTACCCGTGAATACGGCGTGGAGTCGCTGCATAAAACCTTCGCCCTGGCGCAAAAATACGAc    <  1:39241/144‑1 (MQ=255)
                                                                                                                       gTATCCCAACGGTGAAGCGTTGCTGGAAGAGGCGTTACGCTTAGGGGCAGATGTAGTGGGGGCGATTCCGCATTTTGAATTTACCCGTGAATACGGCGTGGAGTCGCTGCATAAAACCTTCGCCCTGGCGCAGAAATACGACCg  >  1:160983/1‑144 (MQ=255)
                                                                                                                                                  |                                                                                                                    
CATGTCGATGTTTCGGATGCAACGCTAACTGCGCTGAAAGCAATGCTGGAAGTGAAGCAGGAAGTCGCGCCGTGGATTGATCTGCAAATCGTCGCCTTCCCTCAGGAAGGGATTTTGTCGTATCCCAACGGTGAAGCGTTGCTGGAAGAGGCGTTACGCTTAGGGGCAGATGTAGTGGGGGCGATTCCGCATTTTGAATTTACCCGTGAATACGGCGTGGAGTCGCTGCATAAAACCTTCGCCCTGGCGCAAAAATACGACCG  >  CP009273/352248‑352510

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑