breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Read File Information

read filereadsbasespassed filtersaveragelongestmapped
errorsSRR7212221_R1.good.fq4,702,997571,322,427100.0%121.5 bases124 bases99.9%
errorsSRR7212221_R2.good.fq4,702,997541,774,268100.0%115.2 bases124 bases99.8%
total9,405,9941,113,096,695100.0%118.3 bases124 bases99.8%

Reference Sequence Information

seq idlengthfit meanfit dispersion% mapped readsdescription
coveragedistributionNC_0029476,181,873180.75.2100.0%Pseudomonas putida KT2440 chromosome, complete genome.
total6,181,873100.0%

fit dispersion is the ratio of the variance to the mean for the negative binomial fit. It is =1 for Poisson and >1 for over-dispersed data.

New Junction Evidence

Junction Candidates Tested

optionlimitactual
Number of alignment pairs examined for constructing junction candidates≤ 100000956
Coverage evenness (position-hash) score of junction candidates≥ 2≥ 2
Test this many junction candidates (n). May be smaller if not enough passed the coverage evenness threshold100 ≤ n ≤ 500073
Total length of all junction candidates (factor times the reference genome length)≤ 0.10.003

Junction Skew Score Calculation

reference sequencepr(no read start)
NC_0029470.50453

pr(no read start) is the probability that there will not be an aligned read whose first base matches a given position on a given strand.

Final Junction Predictions

optionvalue
Coverage evenness (position-hash) score of predicted junctions must be≥ 3
Skew score of predicted junction (−log10 probability of unusual coverage evenness) must be≤ 3
Number of bases that at least one read must overlap each uniquely aligned side of a predicted junction≥ 1

Read Alignment Evidence

optionvalue
ModeConsensus/Mixed Base
Ploidy1 (haploid)
Consensus mutation E-value cutoff10
Consensus frequency cutoff0.75
Consensus minimum variant coverage each strandOFF
Consensus minimum total coverage each strandOFF
Consensus minimum variant coverageOFF
Consensus minimum total coverageOFF
Polymorphism E-value cutoff10
Polymorphism frequency cutoff0.2
Polymorphism minimum variant coverage each strandOFF
Polymorphism minimum total coverage each strandOFF
Polymorphism minimum variant coverageOFF
Polymorphism minimum total coverageOFF
Polymorphism bias cutoffOFF
Predict indel polymorphismsYES
Skip indel polymorphisms in homopolymers runs ofOFF
Skip base substitutions when they create a homopolymer flanked on each side byOFF

Software Versions

programversion
bowtie22.3.4.1
R3.4.4

Execution Times

stepstartendelapsed
Read and reference sequence file input14:36:57 27 Jan 202014:40:56 27 Jan 20203 minutes 59 seconds
Read alignment to reference genome14:40:57 27 Jan 202014:56:27 27 Jan 202015 minutes 30 seconds
Preprocessing alignments for candidate junction identification14:56:27 27 Jan 202015:00:15 27 Jan 20203 minutes 48 seconds
Preliminary analysis of coverage distribution15:00:15 27 Jan 202015:04:39 27 Jan 20204 minutes 24 seconds
Identifying junction candidates15:04:39 27 Jan 202015:04:40 27 Jan 20201 second
Re-alignment to junction candidates15:04:40 27 Jan 202015:09:07 27 Jan 20204 minutes 27 seconds
Resolving best read alignments15:09:07 27 Jan 202015:15:49 27 Jan 20206 minutes 42 seconds
Creating BAM files15:15:49 27 Jan 202015:19:37 27 Jan 20203 minutes 48 seconds
Tabulating error counts15:19:37 27 Jan 202015:22:26 27 Jan 20202 minutes 49 seconds
Re-calibrating base error rates15:22:26 27 Jan 202015:22:28 27 Jan 20202 seconds
Examining read alignment evidence15:22:28 27 Jan 202015:47:06 27 Jan 202024 minutes 38 seconds
Polymorphism statistics15:47:06 27 Jan 202015:47:07 27 Jan 20201 second
Output15:47:07 27 Jan 202015:48:10 27 Jan 20201 minute 3 seconds
Total 1 hour 11 minutes 12 seconds