Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	AE015451	202,276	G→A	8.0%	E2594E (GAG→GAA)	PP_0168 →	putative surface adhesion protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AE015451	202,276	0	G	A	8.0%	177.1 / 4.4	62	E2594E (GAG→GAA)	PP_0168	putative surface adhesion protein
Reads supporting (aligned to +/- strand):  ref base G (29/28);  new base A (3/2);  total (32/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

CTGAGCAACAACGCTCAGGTCACCATCAAGGCCGGTGAGACCAGTGCGCCGTACACCCACGACGCGCAAGGCGATGACGTCTACCAGGACGCTGGCCAGATCAGCCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCACACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCCTCGACGAGGTGGTGGCGAAGCTGACCGCGACCCCTTCGGTCACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAACAACCACTCGGAGCTGTACTTCAA‑GCTGACCGACGGCACCACCGTCGTCGTGGCAGCCAACAGCACCACCGGTTCGGCCACTGCAACCGCCCCGGACAACGTCTATGTCGGCACCAACGCGCCGGTGGTCA  >  AE015451/202073‑202502                                                                                                                                                                                                            |                                                                                                                                                                                                                                    cTGAGCAACAACGCTCAGGTCACCATCAAGGCCGGTGAGACCAGTGCGCCGTACACCCACGACGCGCAAGGCGATGACGTCTACCAGGACGCTGGCCAGATCAGCCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCACACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCCTCGACGAGGTGGTGGCGAAGCTGACCGCGACCCCTTCGGt                                                                                                                                                                                                     >  1:648263/1‑236 (MQ=255)    aGCAACAACGCTCAGGTCACCATCAAGGCCGGTGAGACCAGTGCGCCGTACACCCACGACGCGCAAGGCGATGACGTCTACCAGGACGCTGGCCAGATCAGCCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCACACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCCTCGACGAGGTGGTGGCGAAGCTGACCGCGACCCCTTCGGTCAc                                                                                                                                                                                                  <  1:647262/236‑1 (MQ=255)          aacGCTCAGGTCA‑CATCAAGGCCGGTGAGACCAGTGCGCCGTACACCCACGACGCGCAAGGCGATGACGTCTACCAGGACGCTGGCCAGATCAGCCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCACACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCCTCGACGAGGTGGTGGCGAAGCTGACCGCGACCCCTTCGGTCACCGAAGGc                                                                                                                                                                                           <  1:98229/236‑1 (MQ=255)            cGCTCAGGTCACCATCAAGGCCGGTGAGACCAGTGCGCCGTACACCCACGACGCGCAAGGCGATGACGTCTACCAGGACGCTGGCCAGATCAGCCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCACACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCCTCGACGAGGTGGTGGCGAAGCTGACCGCGACCCCTTCGGTCACCGAAGGCg                                                                                                                                                                                          >  1:5538/1‑236 (MQ=255)               tCAGGTCACCATCAAGGCCGGTGAGACCAGTGCGCCGTACACCCACGACGCGCAAGGCGATGACGTCTACCAGGACGCTGGCCAGATCAGCCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCACACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCCTCGACGAGGTGGTGGCGAAGCTGACCGCGACCCCTTCGGTCACCGAAGGCGGt                                                                                                                                                                                        <  1:492674/235‑1 (MQ=255)               tCAGGTCACCATCAAGGCCGGTGAGACCAGTGCGCCGTACACCCACGACGCGCAAGGCGATGACGTCTACCAGGACGCTGGCCAGATCAGCCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCACACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCCTCGACGAGGTGGTGGCGAAGCTGACCGCGACCCCTTCGGTCACCGAAGGCGGTg                                                                                                                                                                                       <  1:727168/236‑1 (MQ=255)                 aGGTCACCATCAAGGCCGGTGAGACCAGTGCGCCGTACACCCACGACGCGCAAGGCGATGACGTCTACCAGGACGCTGGCCAGATCAGCCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCACACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCCTCGACGAGGTGGTGGCGAAGCTGACCGCGACCCCTTCGGTCACCGAAGGCGGTga                                                                                                                                                                                      >  1:426629/1‑235 (MQ=255)                    tCACCATCAAGGCCGGTGAGACCAGTGCGCCGTACACCCACGACGCGCAAGGCGATGACGTCTACCAGGACGCTGGCCAGATCAGCCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCACACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCCTCGACGAGGTGGTGGCGAAGCTGACCGCGACCCCTTCGGTCACCGAAGGCGGTg                                                                                                                                                                                       >  2:727167/1‑231 (MQ=255)                                                gcCGTACACCCACGACGCGCAAGGCGATGACGTCTACCAGGACGCTGGCCAGATCAGCCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCACACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCCTCGACGAGGTGGTGGCGAAGCTGACCGCGACCCCTTCGGTCACCGAAGGCGGTga                                                                                                                                                                                      >  2:275784/1‑204 (MQ=40)                                                gcCGTACACCCACGACGCGCAAGGCGATGACGTCTACCAGGACGCTGGCCAGATCAGCCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCACACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCCTCGACGAGGTGGTGGCGAAGCTGACCGCGACCCCTTCGGTCACCGAAGGCGGTga                                                                                                                                                                                      <  1:275785/204‑1 (MQ=40)                                                       ccccACGACGCGCAAGGCGATGACGTCTACCAGGACGCTGGCCAGATCAGCCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCACACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCCTCGACGAGGTGGTGGCGAAGCTGACCGCGACCCCTTCGGTCACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGAc                                                                                                                                                    <  1:143471/230‑1 (MQ=37)                                                       acCCACGACGCGCAAGGCGATGACGTCTACCAGGACGCTGGCCAGATCAGCCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCACACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCCTCGACGAGGTGGTGGCGAAGCTGACCGCGACCCCTTCGGTCACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTc                                                                                                                                                <  1:744455/235‑1 (MQ=39)                                                          cACGACGCGCAAGGCGATGACGTCTACCAGGACGCTGGCCAGATCAGCCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCACACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCCTCGACGAGGTGGTGGCGAAGCTGACCGCGACCCCTTCGGTCACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCt                                                                                                                                               >  2:323370/1‑233 (MQ=40)                                                                 cgcAAGGCGATGACGTCTACCAGGACGCTGGCCAGATCAGCCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCACACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCCTCGACGAGGTGGTGGCGAAGCTGACCGCGACCCCTTCGGTCACCGAAGGCgg                                                                                                                                                                                         >  1:595800/1‑184 (MQ=39)                                                                 cgcAAGGCGATGACGTCTACCAGGACGCTGGCCAGATCAGCCTGGGCATCACGTCCGCTGTGGATGTAGACGGACACACCTTCGAGAACCTGCAACTGGGCGGCACCGCGACGGTTCAAGTGACCGACACCCCCGACGAAGTCGTAGCCAAGTTGACCGCCCCCCCGTCGGTCACCGAAGGCGGAGAGATCACCTACACCATCAcg                                                                                                                                                                   >  2:466809/1‑205 (MQ=11)                                                                    aaGGCGATGACGTCTACCAGGACGCTGGCCAGATCAGCCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCACACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCCTCGACGAGGTGGTGGCGAAGCTGACCGCGACCCCTTCGGTCACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACcaacaa                                                                                                                                                        >  2:356001/1‑214 (MQ=39)                                                                                        gACGCTGGCCAGATCAGCTTGGGCATCACGTCGGCGGTGGATGTAGACGGTCCTGCGTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCCTCGACGAGGTGGTGGCGAAGCTGACCGCGACCCCTTCGGTCACCGAAGGCGGTGAGATCACCTACACCATCAcc                                                                                                                                                                   <  1:269107/183‑1 (MQ=11)                                                                                           gCTGGCCAGATCAGCCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCACACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCCTCGACGAGGTGGTGGCGAAGCTGACCGCGACCCCTTCGGTCACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTg                                                                                                                                              >  1:436938/1‑201 (MQ=38)                                                                                           gCTGGCCAGATCAGCCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCACACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCCTCGACGAGGTGGTGGCGAAGCTGACCGCGACCCCTTCGGTCACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTg                                                                                                                                              <  2:436937/201‑1 (MQ=35)                                                                                            cTGGCCAGATCAGCCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCACACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCCTCGACGAGGTGGTGGCGAAGCTGACCGCGACCCCTTCGGTCACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTCCCGATCAACAACCACTCTGAGCTGTAc                                                                                                                   >  1:559765/1‑227 (MQ=25)                                                                                             gggCCAGATCAGCCTCGGCATCACGTCCGCTGTGGATGTAGACGGTCACACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCCTCGACGAAGTCGTGGCGAAGCTGACCGCGACCCCTTCGGTGACCGAAGGCGGCGAGATCACCTACACCATCACGCTGACCAACAAAGACGGTCTGCCGATCAATAACCACTCgg                                                                                                                           >  2:563307/2‑218 (MQ=11)                                                                                               gCCAGATCAGCCTGGGCATCACGTCCGCTGTGGATGTCGACGGTCCCACCTTCGAGAACCTGCCACTGGGCGGCACCGCGAAGGTGGATGTGACCGACACCCGCGACGAGGTCGTGGCCAAGCTGACCGCCAccc                                                                                                                                                                                                            >  2:687328/1‑135 (MQ=11)                                                                                               gCCAGATCAGCCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCACACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCCTCGACGAGGTGGTGGCGAAGCTGACCGCGACCCCTTCGGTCACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTc                                                                                                                                                <  1:377643/195‑1 (MQ=34)                                                                                               gCCAGATCAGCCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCACACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCCTCGACGAGGTGGTGGCGAAGCTGACCGCGACCCCTTCGGTCACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAACAACCACTCGGAGCTGTACTTCAA‑GCTGAc                                                                                                       >  1:757952/1‑235 (MQ=34)                                                                                               gCCAGATCAGCCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCACACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCCTCGACGAGGTGGTGGCGAAGCTGACCGCGACCCCTTCGGTCACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAACAACCACACggc                                                                                                                          >  2:156594/1‑216 (MQ=21)                                                                                               gCCAGATCAGCCTCGGCATCACGTCCGCTGTGGATGTAGACGGTCACACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCCTCGACGAAGTCGTGGCGAAGCTGACCGCGACCCCTTCGGTGACCGAAGGCGGCGAGATCACCTACACCATCACGCTGACCAACAAAGACGGTCTGCCGATCAATAACCACTCGGAGCTGTACTTCAA‑GCTg                                                                                                         <  1:563308/233‑1 (MQ=11)                                                                                                   gATCAGCCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCACACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCCTCGACGAGGTGGTGGCGAAGCTGACCGCGACCCCTTCGGTCACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAACAACCACTCGGAGCTGTACTTCAA‑GCTGACCGACg                                                                                                  <  1:432368/236‑1 (MQ=34)                                                                                                            gggCATCACGTCCGCTGTGGATGTAGACGGTCACACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCCTCGACGAggtg                                                                                                                                                                                                                                  >  2:651767/1‑100 (MQ=31)                                                                                                            gggCATCACGTCCGCTGTGGATGTAGACGGTCACACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCCTCGACGAGGTGGTGGCGAAGCTGACCGCGACCCCTTCGGTCACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAACAACCa                                                                                                                                >  1:111302/1‑198 (MQ=33)                                                                                                            gggCATCACGTCCGCTGTGGATGTAGACGGTCACACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCCTCGACGAGGTGGTGGCGAAGCTGACCGCGACCCCTTCGGTCACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAACAACCACTCGGAGCTGTACTTCAA‑GCTGACCGACGGCaccacc                                                                                          >  1:218798/1‑235 (MQ=33)                                                                                                               aaTCACGTCCGCTGTGGATGTAGACGGTCACACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCCTCGACGAGGTGGTGGCGAAGCTGACCGCGACCCCTTCGGTCACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAACAACCACTCGGAGCTGTACTTCAA‑GCTGACCGACGGCACCACcgtcg                                                                                      <  2:374085/235‑1 (MQ=21)                                                                                                                  cACGTCCGCTGTGGATGTAGACGGTCACACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCCTCGACGAGGTGGTGGCGAAGCTGACCGCGACCCCTTCGGTCACCGAAGGCGGTGAGATCACCTACACCATCACCCTg                                                                                                                                                                <  2:138619/160‑1 (MQ=31)                                                                                                                    cggccGCTGTGGATGTAGACGGTCACACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCCTCGACGAGGTGGTGGCGAAGCTGACCGCGACCCCTTCGGTCACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATcaa                                                                                                                                      <  2:135831/181‑1 (MQ=18)                                                                                                                      tCCGCTGTGGATGTAGCCGGTCACACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGGTCAAGTGACCGACACCCTCGACGAGGTGGTGGCGAAGCTGACCGCGACCCCTTCGGTCACCGa                                                                                                                                                                                               >  2:710836/1‑125 (MQ=17)                                                                                                                      tCCGCTGTGGATGTAGACGGTCACACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCCTCGACGAGGTGGTGGCGAAGCTGACCGCGACCCCTTCGGTCACCGAAGGCGGCGAGATCACCTACACCa                                                                                                                                                                        >  2:79860/1‑148 (MQ=17)                                                                                                                                aTGTAGACGGTCACACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCCTCGACGAGGTGGTGGCGAAGCTGACCGCGACCCCTTCGGTCACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAACAACCACTCGGAGCTGTACTTCAA‑GCTGACCGACGGcac                                                                                              >  2:430855/1‑211 (MQ=32)                                                                                                                                aTGTAGACGGTCACACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCCTCGACGAAGTCGTGGCGAAGCTGACCGCGACCCCTTCGGTGACCGAAGGCGGCGAGATCACCTACACCATCACGCTGACCAACAAAGACGGTCTGCCGATCAACAACCACTCGGAGCTGTACTTCAA‑GCTGACCGACGGCACCACCGTCGTCGTGGCAGCCAACa                                                                       >  2:454537/1‑234 (MQ=255)                                                                                                                                        ggTCACACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCCTCGACGAGGTGGTGGCGAAGCTGACCGCGACCCCTTCGGTCACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAACAACCACt                                                                                                                              <  2:96023/172‑1 (MQ=33)                                                                                                                                             cacCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCCTCGACGAGGTGGTGGCGAAGCTGACCGCGACCCCTTCGGTCACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAACAACCACTCGGAGCTGTACTTCAA‑GCTGACCGACGGCACCACcgtcgtc                                                                                    >  1:703457/1‑208 (MQ=35)                                                                                                                                              cccTTCGAGAACCTGCACCTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCCTCGACGAGGTGGTGGCGAAGCTGACCGCGACCCCTTCGGTCAc                                                                                                                                                                                                  <  2:208396/97‑1 (MQ=18)                                                                                                                                              acCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCCTCGACGAGGTGGTGGCGAAGCTGACCGCGACCCCTTCGGTCACCGAAGGCGGTGAGATCACCTACACCGTCACCCTGAc                                                                                                                                                              >  2:727491/1‑134 (MQ=17)                                                                                                                                               ctttCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCCTCGACGAGGTGGTGGCGAAGCTGACCGCGACCCCTTCGGTCACCGAAg                                                                                                                                                                                             <  1:279625/100‑1 (MQ=17)                                                                                                                                               cgttCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCCTCGACGAGGTCGTGGCGAAGCTGACCGCGACCCCTTCGGTCACCGAAGGCGGTGAGATCa                                                                                                                                                                                 >  2:115113/3‑114 (MQ=17)                                                                                                                                                cTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCCTCGACGAGGTGGTGGCGAAGCTGACCGCGACCCCTTCGGTCAcc                                                                                                                                                                                                 <  2:403908/97‑1 (MQ=33)                                                                                                                                                cTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCCTCGACGAGGTGGTGGCGAAGCTGACCGCGACCCCTTCGGTCACCGAAGGCGGTGAGATCAc                                                                                                                                                                                <  1:115113/114‑1 (MQ=32)                                                                                                                                                    gagaACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGCCAGACACCCTCGACGAGGTGGTGGCGAAGCTGGCCGCGACCCCTTCGGTCACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAACAACCACTCGGAGCTGTACTTCAA‑GCTGACCGACGGCACCACCGTCGTCGTGGCa                                                                              <  1:216869/207‑1 (MQ=21)                                                                                                                                                     agaACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCCTCGACGAGGTGGTGGCGAAGCTGACCGCGACCCCTTCGGTCACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAACAACCACTCGGAGCTGTACTTCAA‑GCTGACCGACgg                                                                                                 >  2:237654/1‑187 (MQ=33)                                                                                                                                                        aCCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCCTCGACGAGGTGGTGGCGAAGCTGACCGCGACCCCTTCGGTCACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAACAACCACTCGGAGCTGTACTTCAA‑GCTGACCGACGGCACCACCGTCGTCGTGGCAGCCAACAGCACCACCGGTTCGGCCACTGCAAcc                                             <  1:323371/236‑1 (MQ=38)                                                                                                                                                           tGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCCTCGACGAGGTGGTGGCGAAGCTGACCGCGACCCCTTCGGTCACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAACAAcc                                                                                                                                 <  1:177119/150‑1 (MQ=34)                                                                                                                                                           tGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCCTCGACGAGGTGGTGGCGAAGCTGACCGCGACCCCTTCGGTCACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAACAAcc                                                                                                                                 >  2:177118/1‑150 (MQ=34)                                                                                                                                                               aCTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCCTCGACGAGGTGGTGGCGAAGCTGACCGCGACCCCTTCGGTCACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAACAACCACTc                                                                                                                             >  2:573812/1‑150 (MQ=34)                                                                                                                                                                       gcAACGCTTCGGTTCAAGTGACCGACACCCTCGACGAGGTGGTGGCGAAGCTGACCGCGACCCCTTCGGTCACCGAAGGCGGTGAGATCACCTACACCATCAcc                                                                                                                                                                   >  1:745813/1‑104 (MQ=33)                                                                                                                                                                       gcAACGCTTCGGTTCAAGTGACCGACACCCTCGACGAGGTGGTGGCGAAGCTGACCGCGACCCCTTCGGTCACCGAAGGCGGTGAGATCACCTACACCATCAcc                                                                                                                                                                   <  2:745812/104‑1 (MQ=33)                                                                                                                                                                        cAACGCTTCGGTTCAAGTGACCGACACCCTCGACGAGGTGGTGGCGAAGCTGACCGCGACCCCTTCGGTCACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAACAACCACTCGGAGCTGTACTTCAA‑GCTGACCGACGGCACCACCGTCGTCGTGGCAGCCAACAGCACCACCGGTTCGGCCACTGCAACCg                                            <  2:757951/221‑1 (MQ=38)                                                                                                                                                                                cGGTTCAAGTGACCGACACCCTCGACGAAGTCGTGGCGAAGCTGACCGCGACCCCTTCGGTCACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAACAACCACt                                                                                                                              <  1:636021/132‑1 (MQ=25)                                                                                                                                                                                        gTGACCGACACCCTCGACGAGGTGGTGGCGAAGCTGACCGCGACCCCTTCGGTCACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAACAACCACTCGGAGCTGTACTTCAA‑GCTGACCGACGGCACCACCGTCGTCGTGGCAGCCAACAGCACCACCGGTTCGGCCACTGCAACCGCCCCg                                       <  1:572653/210‑1 (MQ=255)                                                                                                                                                                                           aCCGACACCCTCGACGAGGTGGTGGCGAAGCTGACCGCGACCCCTTCGGTCACCGAAGGCGGTGAGATCAc                                                                                                                                                                                >  1:483691/1‑71 (MQ=35)                                                                                                                                                                                           aCCGACACCCTCGACGAGGTGGTGGCGAAGCTGACCGCGACCCCTTCGGTCACCGAAGGCGGTGAGATCAc                                                                                                                                                                                <  2:483690/71‑1 (MQ=35)                                                                                                                                                                                              gACACCCTCGACGAGGTGGTGGCGAAGCTGACCGCGACCCCTTCGGTCACCGAAGGCGGTGAGATCACCTACACCATc                                                                                                                                                                      <  2:191267/78‑1 (MQ=34)                                                                                                                                                                                                cacCCTCGACGAGGTGGTGGCGAAGCTGACCGCGACCCCTTCGGTCACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGAAGGTCTGCCGATCAACAACCCCTCGGAGCTGTACTTCAA‑GCTGACCGa                                                                                                    >  2:244161/1‑141 (MQ=17)                                                                                                                                                                                                   ccTCGACGAGGTGGTGGCGAAGCTGACCGCGACCCCTTCGGTCACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAACAACCACTCGGAGCTGTACTTCAA‑GCTGACCGACGGCACCACCGTCGTCGTGGCAGCCAACAGCACCACCGGTTCGGCCACTGCAACCGCCCCGGACAACGTCTATGTCGGCACCAACGCGCCGGTGGTCa  >  1:366499/1‑236 (MQ=40)                                                                                                                                                                                                     tCGACGAGGTGGTGGCGAAGCTGACCGCGACCCCTTCGGTCACCGAAGGCGGTGAGATCACCTACACCATCACCCTGCCCAACAAAGACGGTCTGCCGATCAACAACCACTCGGAGCTGTACTTCAAGGCTGACCGACGGCACCACCGTCGTCGTGGCAGCCAACAGca                                                                    <  2:374705/169‑1 (MQ=255)                                                                                                                                                                                                     tCGACGAGGTGGTGGCGAAGCTGACCGCGACCCCTTCGGTCACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAACAACCACTCGGAGCTGTACTTCAA‑GCTGACCGACGGCACCACCGTCGTCGTGGCAGCCAACAGCACCACCGGTTCGGCCACTGCCACCggcc                                         >  2:572652/1‑192 (MQ=255)                                                                                                                                                                                                            |                                                                                                                                                                                                                                    CTGAGCAACAACGCTCAGGTCACCATCAAGGCCGGTGAGACCAGTGCGCCGTACACCCACGACGCGCAAGGCGATGACGTCTACCAGGACGCTGGCCAGATCAGCCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCACACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCCTCGACGAGGTGGTGGCGAAGCTGACCGCGACCCCTTCGGTCACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAACAACCACTCGGAGCTGTACTTCAA‑GCTGACCGACGGCACCACCGTCGTCGTGGCAGCCAACAGCACCACCGGTTCGGCCACTGCAACCGCCCCGGACAACGTCTATGTCGGCACCAACGCGCCGGTGGTCA  >  AE015451/202073‑202502

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑