Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,617,465 A→G 6.5% T146T (ACA→ACG ydeA → arabinose efflux transporter, arabinose‑inducible

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,617,4650AG 6.5% 190.9 / 3.0 62T146T (ACA→ACGydeAarabinose efflux transporter, arabinose‑inducible
Reads supporting (aligned to +/- strand):  ref base A (26/31);  new base G (2/2);  total (29/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.37e-01

CATCATGTTGACCATTTACGCATGGGTAGTAGCGCTAATGTCATTGCCTTTTATGTTAATGACCAGTCAGGTTGAACGGCGCAAATTACTGATCTGCCTGTTTGTGGTGTTTATTGCCAGCCACGTACTGTCGTTTTTGTCGTGGAGCTTTACCGTTCTGGTGATCAGTCGCATTGGTGTGGCTTTTGCACATGCGATTTTCTGGTCGATTACGGCGTCTCTGGCGATCCGTATGGCTCCGGCCGGGAAGCGAGCACAGGCATTGAGTTTAATTGCCACCGGTACAGCACTGGCGATGGTCTTAGGTTTACCTCTCGGGCGCATTGTGGGCCAGTATTTCGGTTGGCGAATGACCTTCTTCGCGATTGGTATTGGGGCGCTTATCACCCTTTTGTGCCTGATTAAGTTACTTCCCTTACTGCCCAGTGAGCATTCCGGTTCACTGAAAAGCCTCCCGCTATTGTTCCGCCGCCCGGCATTGATGAGCATTTATTTGTTAACTGTGGTGGTTGTCACCGCCCATTACACGGCATACAGCTATATCGAGCCTTTTGTACAAAACAT  >  NC_000913/1617180‑1617743
                                                                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                                                      
catcatGTTGACCATTTCCGCATGGGTAGTAGCGCTAATGTCATAGCCTTTTATGTTAATGACCAGTCAGGTTGAACGGCGCAAATTACTGATCTGCCTGTTTGTGGTGTTTATTGCCAGCCACGTACTGTCGTTTTTGTCGTGGAGCTTTACCGTTCTGGTGATCCGCCGCATGGGTGTGGCTTTTGCACATGCGATTTTCGGGTCGCTTACGGCTGCTCTGGCGTTCCGTCTGGCTCCGGCTGCGATGCGAGCCAAGGACTTGAGTCACATTGTAACCGCTACAGc                                                                                                                                                                                                                                                                                      >  2:570504/1‑288 (MQ=255)
 atcatGTTGACCATTTACGCATGGGTAGTAGCGCTAATGTCATTGCCTTTTATGTTAATGACCAGTCAGGTTGAACGGCGCAAATTACTGATCTGCCTGTTTGTGGTGTTTATTGCCAGCCACGTACTGTCGTTTTTGTCGTGGAGCTTTACCGTTCTGGTGATCAGTCGCATTGGTGTGGCTTTTGCACATGCGATTTTCTGGTCGATTACGGCGTCTCTGGCGATCCGTATGGCTCCGGCCGGGAAGCGAGCACAGGCATTGAGTTTAATTGCCACCGGTACAGCa                                                                                                                                                                                                                                                                                     <  1:648852/288‑1 (MQ=255)
     tGTTGACCATTTACGCATGGGTAGTAGCGCTAATGTCATTGCCTTTTATGTTAATGACCAGTCAGGTTGAACGGCGCAAATTACTGATCTGCCTGTTTGTGGTGTTTATTGCCAGCCACGTACTGTCGTTTTTGTCGTGGAGCTTTACCGTTCTGGTGATCAGTCGCATTGGTGTGGCTTTTGCACATGCGATTTTCTGGTTGATTACGGCGTCTCTGGCGATCCGTATGGCTCCTGCCGGGAAGCGAGCACGGGCATTGACTTTAATTGCTACCGGTCCAGCACTggg                                                                                                                                                                                                                                                                                >  2:562356/1‑288 (MQ=255)
       ttGACCATTTACGAATGGGTGGTAGCGCTAATTTCATTTCCTTTTATGTTAATGACCAGTCAGGTTGAACGGCGCAAATTACTGATCTGCCTGTTTGTGGTGTTTATTGCCAGCCACGTACTGTCGTTTTTGTCGTGGAGCTTTACCGTTCTGGTGATCAGTCGCATTGGTGTGGCTTTTGCACATGCGATTTTCTGGTCGATTACGGCGTCTCTGGCGATCCGTATGGCTCCGGCCGGGAAGCGAGCACAGGCATTGAGTTTAATTGCCACCGGTACAGCACTGGCGa                                                                                                                                                                                                                                                                              <  2:203068/289‑1 (MQ=255)
                      tGGGTAGTAGCGCTAATGTCATTGCCTTTTATGTTAATGACCAGTCAGGTTGAACGGCGCAAATTACTGATCTGCCTGTTTGTGGTGTTTATTGCCAGCCACGTACTGTCGTTTTTGTCGTGGAGCTTTACCGTTCTGGTGATCAGTCGCATTGGTGTGGCTTTTGCACATGCGATTTTCTGGTCGATTACGGCGTCTCTGGCGATCCGTATGGCCCCGGCCGGGAAGCGCGCACCGGCATGGGCTTTACTTGCCACAGGTACAGTACTTGGGAGTGTCTCGGGTTTa                                                                                                                                                                                                                                                                >  2:522698/1‑288 (MQ=255)
                               gcgcTAATGTCATTGCCTTTTATGTTAATGACCAGTCAGGTTGAACGGCGCAAATTACTGATCTGCCTGTTTGTGGTGTTTATTGCCAGCCACGTACTGTCGTTTTTGTCGTGGAGCTTTACCGTTCTGGTGATCAGTCGCATTGGTGTGGCTTTTGCACATGCGATTTTCTGGTCGATTACGGCGTCTCTGGCGATCCGTATGGCTCCGGCCGGGAAGCGAGCACAGGCATTGAGTTTAATTGCCCCCGGTACAGCACTGGCGAGGGTCTGAGGTTTACATATCGGgc                                                                                                                                                                                                                                                      >  2:414389/1‑289 (MQ=255)
                                 gcTAATGTCATTGCCTTTTATGTTAATGACCAGTCAGGTTGAACGGCGCAAATTACTGATCTGCCTGTTTGTGGTGTTTATTGCCAGCCACGTACTGTCGTTTTTGTCGTGGAGCTTTACCGTTCTGGTGATCAGTCGCATTGGTGTGGCTTTTGCACATGCGATTTTCTGGTCGATTACGGCGTCTCTGGCGATCCGTATGGCTCCGGCCGGGACGCGAGCACAGGCCTTGAGTTTAATTGCCACCGGTACAGCACTGGCGATGGTCTTAGGTTTACCTTCCGGgcc                                                                                                                                                                                                                                                     >  2:92087/1‑287 (MQ=255)
                                 gcTAATGTCATTGCCTTTTATGTTAATGACCAGTCAGGTTGAACGGCGCAAATTACTGATCTGCCTGTTTGTGGTGTTTATTGCCAGCCACGTACTGTCGTTTTTGTCGTGGAGCTTTACCGTTCTGGTGATCAGTCGCATTGGTGTGGCTTTTGCACATGCGATTTTCTGGTCGATTACGGCGTCTCTGGCGATCCGTATGGCTCCGGCCGGGAAGCGAGCACAGGCATTGAGTTTAATTGCCACCGGTACAGCACTGGCGATGGTCTTAGGTTTACCTCTCGGgcg                                                                                                                                                                                                                                                     <  1:551389/288‑1 (MQ=255)
                                 gcTAATGTCATTGCCTTTTATGTTAATGACCAGTCAGGTTGAACGGCGCAAATTACTGATCTGCCTGTTTGTGGTGTTTATTGCCAGCCACGTACTGTCGTTTTTGTCGTGGAGCTTTACCGTTCTGGTGATCAGTCGCATTGGTGTGGCTTTTGCACATGCGATTTTCTGGTCGATTACGGCGTCTCTGGCGATCCGTATGGCTCCGGCCGGGAAGCGAGCACAGGCATTGAGTTTAATTGCCACCGGTACAGCACTGGCGATGGTCTTAGGTTTACCTCTCGGgcg                                                                                                                                                                                                                                                     <  1:412904/288‑1 (MQ=255)
                                           ttGCCTTTTATGTTAATGACCAGTCAGGTTGAACGGCGCAAATTACTGATCTGCCTGTTTGTGGTGTTTATTGCCAGCCACGTACTGTCGTTTTTGTCGTGGAGCTTTACCGTTCTGGTGATCAGTCGCATTGGTGTGGCTTTTGCACATGCGATTTTCTGGTCGATTACGGCGTCTCTGGCGATCCGTATGGCTCCGGCCGGGAAGCGAGCACAGGCATTGAGTTTAATTGCCACCGGTACAGCACTGGCGATGGTCTTAGGTTTACCTCTCGGGCGCATTGTGGGcc                                                                                                                                                                                                                                          <  1:208943/289‑1 (MQ=255)
                                                ttttATGTTAATGACCCGTCAGGTTGAACGGCGCAAATTACTTATCTGCCTGTTTGTGGTGTTTATTGCCAGCCACGTACTGTCTTTTTTGTCGTGGAGCTTTACCGTTCTGGTGATCAGTCGCATTGGTGTGGCTTTTGCACATGCGATTTTCTGGTCGATTCCGGCGTCTCTGGCGATCCGTATGGCTCCGGCCGGGAAGCGAGCACAGGCATTGAGTTTAATTGCCACCGGTACAGCACTGGCGATGGTCTTAGGTTTACCTCTCGGGCGCATTGTgggtataggt                                                                                                                                                                                                                                     <  1:118035/289‑8 (MQ=255)
                                                                      gTTGAACGGCGCAAATTACTGATCTGCCTGTTTGTGGTGTTTATTGCCAGCCACGTACTGTCGTTTTGGTCGTGGAGCTTTACCGTTCTGGTGATCAGTCGCATTGGTGTGGCTTTTGCACATGCGATTTTCTGGTCGATTACGGCGTCTCTGGCGATCCGTATGGCTCCGGCCGGGAAGCGAGCACAGGCATTGAGTTTAATTGTCACCGGTACAGCACTGGGGATGGTCTTGGGTTTACTTCTCGGGCGCATTGTGGGCCGGTATTCTGGTTGGCGTATGGCtttct                                                                                                                                                                                                               >  2:97577/1‑289 (MQ=255)
                                                                            cGGCGCAAATTACTGATCTGCCTGTTTGTGGTGTTTATTGCCAGCCACGTACTGTCGTTTTTGTCGTGGAGCTTTACCGTTCTGGTGATCAGTCGCATTGGTGTGGCTTTTGCACATGCGATTTTCTGGTCGATTACGGCGTCTCTGGCGATCCGTATGGCTCCGGCCGGGAAGCGAGCACAGGCATTGAGTTTAATTGCCACCGGTACAGCACTGGCGATGGTCTTAGGTTTACCTCTCGGGCGCATTGTGGGCCAGTATTTCGGTTGGCGAATGACCTTCTTCGCGa                                                                                                                                                                                                         >  1:195252/1‑289 (MQ=255)
                                                                            cGGCGCAAATTACTGATCTGCCTGTTTGTGGTGTTTATTGCCAGCCACGTACTGTCGTTTTTGTCGTGGAGCTTTACCGTTCTGGTGATCAGTCGCATTGGTGTGGCTTTTGCACATGCGATTTTCTGGTCGATTACGGCGTCTCTGGCGATCCGTATGGCTCCGGCCGGGAAGCGAGCACAGGCATTGAGTTTAATTGCCACCGGTACAGCACTGGCGATGGTCTTAGGTTTACCTCTCGGGCGCATTGTGGGCCAGTATTTCGGTTGGCGAATGACCTTCTTCGCGa                                                                                                                                                                                                         >  1:272470/1‑289 (MQ=255)
                                                                             ggggcAAATTACTGATCTGCCGGTTTGTGGTTTTTATTGCCAGCCACGTACTGTCGTTTTTGTCGTGGAGCTTTACCGTTCTGGTGATCAGTCGCATTGGTGTGGCTTTTGCACATGCGATTTTCTGGTCGATTACGGCGTCTCTGGCGATCCGTATGGCTCCGGCCGGGAAGCGAGCACAGGCATTGAGTTTAATTGCCACCGGTACAGCACTGGCGATGGTCTTAGGTGTACCTCTCGGGCGCATTGTGGGCCAGTATTTCGGTTGGCGAATGACCTTCTTCGCGAt                                                                                                                                                                                                        <  2:473105/286‑1 (MQ=255)
                                                                             ggCGCAAATTACTGATCTGCCTGTTTGTGGTGTTTATTGCCAGCCACGTACTGTCGTTTTTGTCGTGGAGCTTTACCGTTCTGGTGATCAGTCGCATTGGTGTGGCTTTTGCACATGCGATTTTCTGGTCGATTACGGCGTCTCTGGCGATCCGTATGGCTCCGGCCGGGAAGCGAGCACAGGCATTGAGTTTAATTGCCACCGGTACAGCACTGGCGATGGTCTTAGGTTTACCTCTCGGGCGCATTGTGGGCCAGTATTTCGGTTGGCGAATGACCTTCTTCGCGAt                                                                                                                                                                                                        <  1:522698/289‑1 (MQ=255)
                                                                             gccgcAAATTACTGATCTGCCTGTTTGTGGTGTTTATTGCCAGCCACGTACTGTCGTTTTTGTCGTGGAGCTTTACCGTTCTGGTGATCAGTCGCATTGGTGTGGCTTTTGCACATGCGATTTTCTGGTCGATTACGGCGTCTCTGGCGATCCGTATGGCTCCGGCCGGGAAGCGAGCACAGGCATTGAGTTTAATTGCCACCGGTACAGCACTGGCGATGGTCTTAGGTTTACCTCTCGGGCGCATTGTGGGCCAGTATTTCGGTTGGCGAATGACCTTCTTCGCGAt                                                                                                                                                                                                        <  2:525401/287‑1 (MQ=255)
                                                                              gcgcAAATTACTGATCTGCCTGTTTGTGGTGTTTATTGCCAGCCACGTACTGTCGTTTTTGTCGTGGAGCTTTACCGTTCTGGTGATCAGTCGCATTGGTGTGGCTTTTGCACATGCGATTTTCTGGTCGATTACGGCGTCTCTGGCGATCCGTATGGCTCCGGCCGGGAAGCGAGCACAGGCATTGAGTTTAATTGCCACCGGTACAGCACTGGCGATGGTCTTAGGTTTACCTCTCGGGCGCATTGTGGGCCAGTATTTCGGTTGGCGAATGACCTTCTTCGCGAt                                                                                                                                                                                                        <  2:239565/288‑1 (MQ=255)
                                                                                        cTGATCTGCCTGTTTGTGGTGTTTATTGCCAGCCACGTACTGTCGTTTTTGTCGTGGAGCTTTACCGTTCTGGTGATCAGTCGCATTGGTGTGGCTTTTGCACATGCGATTTTCTGGTCGATTACGGCGTCTCTGGCGATCCGTATGGCTCCGGCCGGGAAGCGAGCACAGGCATTGAGTTTAATTGCCACCGGTACAGCACTGGCGATGGTCTTAGGTTTACCTCTCGGGCGCATTGTGGGCCAGTATTTCGGTTGGCGAATGACCTTCTTCGCGATTGGTATTgggg                                                                                                                                                                                             <  1:133052/289‑1 (MQ=255)
                                                                                        cTGATCTGCCTGTTTGTGGTGTTTATTGCCAGCCACGTACTGTCGTTTTTGTCGTGGAGCTTTACCGTTCTGGTGATCAGTCGCATTGGTGTGGCTTTTGCACATGCGATTTTCTGGTCGATTACGGCGTCTCTGGCGATCCGTATGGCTCCGGCCGGGAAGCGAGCACAGGCATTGAGTTTAATTGCCACCGGTACAGCACTGGCGATGGTCTTAGGTTTACCTCTCGGGCGCATTGTGGGCCAGTATTTCGGTTGGCGAATGACCTTCTTCGCGATTGGTATTgggg                                                                                                                                                                                             <  1:64129/289‑1 (MQ=255)
                                                                                                ccTGTTTGTGGTGTTTATTGCCAGCCACGTACTGTCGTTTTTGTCGTGGAGCTTTACCGTTCTGGTGATCAGTCGCATTGGTGTGGCTTTTGCACATGCGATTTTCTGGTCGATTACGGCGTCTCTGGCGATCCGTATGGCTCCGGCCGGGAAGCGAGCCCAGGCATTGAGTTTAATTGCCACCGGTACAGCACTGGCGCTGGTCTTAGGCTTACCTCTCGGGCGCATTGTGGGCCAGTATTTCGGTTGGCGAATGACCTTCTTCGCGATTGGTATTGGGGCGCTTAt                                                                                                                                                                                      >  1:607410/1‑288 (MQ=255)
                                                                                                ccTGTTTGTGGTGTTTATTGCCAGCCACGTACTGTCGTTTTTGTCGTGGAGCTTTACCGTTCTGGTGATCAGTCGCATTGGTGTGGCTTTTGCACATGCGATTTTCTGGTCGATTACGGCGTCTCTGGCGATCCGTATGGCTCCGGCCGGGAAGCGAGCACAGGCATTGCGTTTAATTGCCACCGGTACAGCACTGGCGATGGCCTTAGGTTTACCTCTCGGGCGCATTGTGGGCCAGTATTTCGGTTGGCGAATGACCGTCTTCGCGATTGGTATTGGGTCGCTTAta                                                                                                                                                                                     >  2:713220/1‑288 (MQ=255)
                                                                                                          gtgtTTATTGCCAGCCACGTACTGTCGTTTTTGTCGTGGAGCTTTACCGTTCTGGTGATCAGTCGCATTGGTGTGGCTTTTGCACATGCGATTTTCTGGTCGATTACGGCGTCTCTGGCGATCCGTATGGCTCCGGCCGGGAAGCGAGCACAGGCATTGAGTTTAATTGCCACCGGTACAGCACTGGCGATGGTCTTAGGTTTACCTCTCGGGCGCATTGTGGGCCAGTATTTCGGTTGGCGAATGACCTTCTTCGCGATTGGTATTGGGGGGTTTATCACCCTTTtgt                                                                                                                                                                           >  1:255912/1‑289 (MQ=255)
                                                                                                                  tGCCAGCCACGTACTGTCGTTTTTGTCGTGGAGCTTTACCGTTCTGGTGATCAGTCGCATTGGTGTGGCTTTTGCACATGCGATTTTCTGGTCGATTACGGCGTCTCTGGCGATCCGTATGGCTCCGGCCGGGAAGCGAGCACAGGCATTGAGTTTAATTGCCACCGGTACAGCACTGGCGATGGTCTTAGGTTTACCTCCCGGGCGCATTGTGGGCCAGTATTTCGGTTGGCGAATGACCTTCCTCGCGATTGGTATTGGGGCGCTTCTCACCCTTTTTTGCCTGAtt                                                                                                                                                                   >  2:101060/1‑289 (MQ=255)
                                                                                                                       gccaCGTACTGTCGTTTTTGTCGTGGAGCTTTACCGTTCTGGTGATCAGTCGCATTGGTGTGGCTTTTGCACATGCGATTTTCTGGTCGATTACGGCGTCTCTGGCGATCCGTATGGCTCCGGCCGGGAAGCGAGCACAGGCATTGAGTTTAATTGCCACCGGTACGGCACTGGCGATGGTCTTAGGTTTACCTCTCGGGCGCATTGTGGGCCAGTATTTCGGTTGGCGAATGACCTTCTTCGCGATTGGTATTGGGGCGCTTATCACCCTTTTGTGCCTGATTAAGtt                                                                                                                                                              >  1:666304/1‑289 (MQ=255)
                                                                                                                         caCGTACTGTCGTTTTTGTCGTGGAGCTTTACCGTTCTGGTGGTCAGTCGCATTGGTGTGGCTTTTGCACATGCGATTTTCTGGTCGATTACGGCGTCTCTGGCGATCCGTATGGCTCCGGCCGGGAAGCGAGCACAGGCATTGAGTTTAATTGCCACCGGTACAGCACTGGCGATGGTCTTAGGTTTACCTCTCGGGCGCATTGTGGGCCAGTATTTCGGTTGGCGAATGACCTTCTTCGCGATTGGTATTGGGGCGCTTATCACCCTTTTGTGCCTGATTAAGTTAc                                                                                                                                                            <  1:713220/289‑1 (MQ=255)
                                                                                                                          aCGTACTGTCGTTTTTGTCGTGGAGCTTTACCGTTCTGGTGATCAGTCGCATTGGTGTGGCTTTTGCACATGCGATTTTCTGGTCGATTACGGCGTCTCTGGCGATCCGTATGGCTCCGGCCGGGAAGCGAGCACAGGCATTGAGTTTAATTGCCACCGGTACAGCACTGGCGATGGTCTTAGGTTTACCTCTCGGGCGCATTGTGGGCCAGTATTTCGGTTGGCGAATGACCTTCTTCGCGATTGGTATTGGGGCGCTTATCACCCTTTTGTGCCTGATTAAGTTACt                                                                                                                                                           <  1:397167/289‑1 (MQ=255)
                                                                                                                                  tCGTTTTTGTCGTGGAGCTTTACCGTTCTGGTGATCAGTCGCATTGGTGTGGCTTTTGCACATGCGATTTTCTGGTCGATTACGGCGTCTCTGGCGATCCGTATGGCTCCGGCCGGGAAGCGAGCACAGGCATTGAGTTTAATTGCCACCGGTACAGCACTGGCGATGGTCTTAGGTTTACCTCTCGGGCGCATTGTGGGCCAGTATTTCGGTTGGCGAATGACCTTCTTCGCGATTGGTATTGGGGCGCTTATCACCCTTTTGTGCCTGATTAAGTTACTTCCCtt                                                                                                                                                     >  1:64559/1‑287 (MQ=255)
                                                                                                                                  tCGTTTTTGTCGTGGAGCTTTACCGTTCTGGTGATCAGTCGCATTGGTGTGGCTTTTGCACATGCGATTTTCTGGTCGATTACGGCGTCTCTGGCGATCCGTATGGCTCCGCCCGGGAAGCGAGCACAGGCATTGAGTTTAATTGCCACCGGTACCGCACTGGCGATGGTCTTAGGTTTACCTCTCGGGCGCCTTGTGGGCCAGTTTTTCGGTTGGCGAATGACCTTCTTCGCGATTGGTATGGGGGCGCTTATCACCCTTTTGTTCCAGATTAAGTTTCTTCACtt                                                                                                                                                     >  2:287211/1‑287 (MQ=255)
                                                                                                                                                gAGCTTTACCGTTCTGGTGATCAGTCGCATTGGTGTGGCTTTTGCACATGCGATTTTCTGGTCGATTACGGCGTCTCTGGCGATCCGTATGGCTCCGGCCGGGAAGCGAGCACAGGCATTGAGTTTAATTGCCACCGGTACAGCACTGGCGATGGTCTTAGGTTTACCTCTCGGGCGCATTGTGGGCCAGTATTTCGGTTGGCGAATGACCTTCTTCGCGATTGGTATTGGGGCGCTTATCACCCTTTTGTGCCTGATTAAGTTACTTCCCTTACTGCCCAGTGAGCa                                                                                                                                      <  1:258067/288‑1 (MQ=255)
                                                                                                                                                gAGCTTTACCGTTCTGGTGATCAGTCGCATTGGTGTGGCTTTTGCACATGCGATTTTCTGGTCGATTACGGCGTCTCTGGCGATCCGTATGGCTCCGGCCGGGAAGCGAGCACAGGCATTGAGTTTAATTGCCACCGGTACAGCACTCGCGCTGGTCTTAGGTTTCGCTCTCGGGCGCATTGTGCGCCAGTATTTCGGTTGTCGACTGACCTTCTTCGCGATTGGTATGGGGGCGCGTATCACGCTTTCGTGTCTGACTTATTTCCTTCCATCACTGCCCAGTGATCAt                                                                                                                                     >  2:547534/1‑289 (MQ=255)
                                                                                                                                                     ttACCGTTCTGGTGATCAGTCGCATTGGTGTGGCTTTTGCACATGCGATTTTCTGGTCGATTACGGCGTCTCTGGCGATCCGTATGGCTCCGGCCGGGAAGCGAGCACAGGCATTGAGTTTAATTGCCACCGGTACAGCACTGGCGATGGTCTTAGGTTTACCTCTCGGGCGCATTGTGGGCCAGTATTTCGGTTGGCGAATGACCTTCTTCGCGATTGGTATTGGGGCGCTTATCACCCTTTTGTGCCTGATTAAGTTACTTCCCTTACTGCCCAGTGAGCATTCCgg                                                                                                                                >  1:505721/1‑289 (MQ=255)
                                                                                                                                                                         cGCATTGGTGTGGCTGTTGCACATGCGATTTTCTGGTCGATTACGGCGTCTCTGGCGATCCGTATGGCTCCGGCCGGGAAGCGAGCACAGGCATTGAGTTTAATTGCCACCGGTACAGCACTGGCGATGGTCTTAGGTTTACCTCTCGGGCGCATTGTGGGCCAGTATTTCGGTTGGCGAATGACCTTCTTCGCGATTGGTATTGGGGCGCTTATCACCCTTTTGTGCCTGATTAAGTTACTTCCCTTACTGCCCAGTGAGCATTCCGGTTCACTGAAAAGCCTcc                                                                                                               <  1:562356/286‑1 (MQ=255)
                                                                                                                                                                          gCATTGGTGTGGCTTTTGCACATGCGATTTTCTGGTCGATTACGGCGTCTCTGGCGATCCGTATGGCTCCGGCCGGGAAGCGAGCACAGGCATTGAGTTTAATTGCCACCGGTACAGCACTGGCGATGGTCTTAGGTTTACCTCTCGGGCGCATTGTGGGCCAGTATTTCGGTTGGCGAATGACCTTCTTCGCGATTGGTATTGGGGCGCTTATCACCCTTTTGTGCCTGATTAAGTTACTTCCCTTACTGCCCAGTGAGCATTCCGGTTCACTGAAAAGCCTCCCGCt                                                                                                           >  2:501136/1‑289 (MQ=255)
                                                                                                                                                                                        tttGCACATGCGATTTTCTGGTCGATTACGGCGTCTCTGGCGATCCGTATGGCTCCGGCCGGGAAGCGAGCACAGGCATTGAGTTTAATTGCCACCGGTACAGCACTGGCGATGGTCTTAGGTTTACCTCTCGGGCGCATTGTGGGCCAGTATTTCGGTTGGCGAATGACCTTCTTCGCGATTGGTATTGGGGCGCTTATCACCCTTTTGTGCCTGATTAAGTTACTTCCCTTACTGCCCAGTGAGCATTCCGGTTCACTGAAAAGCCTCCCGCTATTGTTCCgccgcc                                                                                             <  1:287211/289‑1 (MQ=255)
                                                                                                                                                                                        tttGCACATGCGATTTTCGGGTCGATTACGGGTTCTCTGGCGATCCGTATGGCTCCGGCCGGGAAGCGAGCACAGGCATTGAGTTTAATTGCCACCGGTACAGCACTGGCGATGGTCTTAGGTTTACCTCTCGGGCGCATTGTGGGCCAGTATTTCGGTTGGCGAATGACCTTCTTCGCGATTGGTATTGGGGCGCTTATCACCCTTTTGTGCCTGATTAAGTTACTTCCCTTACTGCCCAGTGAGCATTCCGGTTCACTGAAAAGCCTCCCGCTATTGTTCCgccgcc                                                                                             <  2:505721/289‑1 (MQ=255)
                                                                                                                                                                                          tGCACATGCGATTTTCTGGTCGATTACGGCGTCTCTGGCGATCCGTATGGCTCCGGCCGGGAAGCGAGCACAGGCATTGAGTTTAATTGCCACCGGTACGGCACTGGCGATGGTCTTAGGTTTACCTCTCGGGCGCATTGTGGGCCAGTATTTCGGTTGGCGAATGACCTTCTTCGCGATTGGTATTGGGGCGCTTATCACCCTTTTGTGCCTGATTAAGTTACTTCCCTTACTGCCCAGTGAGCATTCCGGTTCACTGAAAAGCCTCCCGCTATTGTTCCGCCGCCCg                                                                                           >  1:662612/1‑289 (MQ=255)
                                                                                                                                                                                          tGCACATGCGATTTTCTGGTCGATTACGGCGTCTCTGGCGATCCGTATGGCTCCGGCCGGGAAGCGAGCACAGGCATTGAGTTTAATTGCCACCGGTACAGCACTGGCGATGGTCTTAGGTTTACCTCTCGGGCGCATTGTGGGCCAGTATTTCGGTTGGCGAATGACCTTCTTCGCGATTGGTATTGGGGCGCTTATCACCCTTTTGTGCCTGATTACGTTACTTCCCTTACTGCCCAGTGAGCATTCCGGTTCACTGAACAGCCTCCCGCTATATTTCCGTCGCCCg                                                                                           >  2:162437/1‑289 (MQ=255)
                                                                                                                                                                                                   gATTTTCTGTTCGATTACGGCGTCTCTGGCGAGCCGTATGGCTCCGGCCTGGAAGCGAGCACAGGCATTGAGTTGAATTGCCACCGGTACAGCACTGGCGATGGTCTTAGGTTTACCTCTCGGGCGCATTGTGGGCCAGTATTTCGGTTGGCGAATGACCTTCTTCGCGATTGGTATTGGGGCGCTTATCACCCTTTTGTGCCTGATTAAGTTACTTCCCTTACTGCCCAGTGAGCATTCCGGTTCACTGAAAAGCCTCCCGCTATTGTTCCGCCGCCCGGCATtgat                                                                                   <  2:272470/288‑1 (MQ=255)
                                                                                                                                                                                                   gATTGTCTGGTGGATTACGGCGTTGCTGGCGATCCGTATGGCTCCGGCCGGGAAGCGAGCACAGGCATTGAGTTTAATTGCCACCGGTACGGCACTGGCGATGGTCTTAGGTTTACCTCTCGGGCGCATTGTGGGCCAGTATTTCGGTTGGCGAATGACCTTCTTCGCGATTGGTATTGGGGCGCTTATCACCCTTTTGTGCCTGATTAAGTTACTTCCCTTACTGCCCAGTGAGCATTCCGGTTCACTGAAAAGCCTCCCGCTATTGTTCCGCCGCCCGGCATtgat                                                                                   <  2:662612/288‑1 (MQ=255)
                                                                                                                                                                                                         cTGGTCGATTACGGCGTCTCTGGCGATCCGTATGGCTCCGGCCGGGAAGCGAGCACAGGCATTGAGTTTAATTGCCACCGGTACAGCACTGGCGATGGTCTTAGGTTTACCTCTCGGGCGCATTGTGGGCCAGTATTTCGGTTGGCGAATGACCTTCTTCGCGATTGGTATTGGGGCGCTTATCACCCTTTTGTGCCTGATTAAGTTACTTCCCTTACTGCCCAGTGAGCATTCCGGTTCACTGAAAAGCCTCCCGCTATTGTTCCGCCGCCCGGCATTGATGAGCatt                                                                            <  1:162437/289‑1 (MQ=255)
                                                                                                                                                                                                           ggTCGATTACGGCGTCTCTGGCGATCCGTATGGCTCCGGCCGGGAAGCGAGCACAGGCATTGAGTTTAATTGCCACCGGTACAGCACTGGCGATGGTCTTAGGTTTACCTCTCGGGCGCATTGTGGGCCAGTATTTCGGTTGGCGAATGACCTTCTTCGCGATTGGTATTGGGGCGCTTATCACCCTTTTGTGCCTGATTAAGTTACTTCCCTTACTGCCCAGTGAGCATTCCGGTTCACTGAAAAGCCTCCCGCTATTGTTCCGCCGCCCGGCATTGATGAGCattta                                                                          >  1:245206/1‑289 (MQ=255)
                                                                                                                                                                                                                           tctGGCGATCCGTATGGCTCCGGCCGGGAAGCGAGCACAGGCATTGAGTTTAATTGCCACCGGTACAGCACTGGCGATGGTCTTAGGTTTACCTCTCGGGCGCATTGTGGGCCAGTATTTCGGTTGGCGAATGACCTTCTTCGCGAt                                                                                                                                                                                                        <  1:694269/147‑1 (MQ=255)
                                                                                                                                                                                                                           tctGGCGATCCGTATGGCTCCGGCCGGGAAGCGAGCACAGGCATTGAGTTTAATTGCCACCGGTACAGCACTGGCGATGGTCTTAGGTTTACCTCTCGGGCGCATTGTGGGCCAGTATTTCGGTTGGCGAATGACCTTCTTCGCGAt                                                                                                                                                                                                        >  2:694269/1‑147 (MQ=255)
                                                                                                                                                                                                                           tctGGCGATCCGTATGGCTCCGGCCGGGAAGCGAGCACAGGCATTGAGTTTAATTGCCACCGGTACAGCACTGGCGATGGTCTTAGGTTTACCTCTCGGGCGCATTGTGGGCCAGTATTTCGGTTGGCGAATGACCTTCTTCGCGATTGGTATTGGGGCGCTTATCACCCTTTTGTGCCTGATTAAGTTACTTCCCTTACTGCCCAGTGAGCATTCCGGTTCACTGAAAAGCCTCCCGCTATTGTTCCGCCGCCCGGCATtgat                                                                                   >  1:13486/1‑264 (MQ=255)
                                                                                                                                                                                                                           tctGGCGATCCGTATGGCTCCGGCCGGGAAGCGAGCACAGGCATTGAGTTTAATTGCCACCGGTACAGCACTGGCGATGGTCTTAGGTTTACCTCTCGGGCGCATTGTGGGCCAGTATTTCGGTTGGCGAATGACCTTCTTCGCGATTGGTATTGGGGCGCTTATCACCCTTTTGTGCCTGATTAAGTTACTTCCCTTACTGCCCAGTGAGCATTCCGGTTCACTGAAAAGCCTCCCGCTATTGTTCCGCCGCCCGGCATtgat                                                                                   <  2:13486/264‑1 (MQ=255)
                                                                                                                                                                                                                                   tCCGTATGGCTCCGGCCGGGAAGCGAGCACAGGCATTGAGTTTAATTGCCACCGGTACAGCACTGGCGATGGTCTTAGGTTTACCTCTCGGGCGCATTGTGGGCCAGTATTTCGGTTGGCGAATGACCTTCTTCGCGATTGGTATTGGGGCGCTTATCACCCTTTTGTGCCTGATTAAGTTACTTCCCTTACTGCCCAGTGAGCATTCCGGTTCACTGAAAAGCCTCCCGCTATTGTTCCGCCGCCCGGCATTGATGAGCATTTATTTGTTAACTGTGGTGGTTGTCa                                                   <  2:64559/288‑1 (MQ=255)
                                                                                                                                                                                                                                       tATGGCTCCGGCCGGGAAGCGAGCACAGGCATTGAGTTTAATTGCCACCGGTACAGCACTGGCGATGGTCTTAGGTTTACCTCTCGGGCGCATTGTGGGCCAGTATTTCGGTTGGCGAATGACCTTCTTCGCGATTGGTATTGGGGCGCTTATCACCCTTTTGTGCCTGATTAAGTTACTTCCCTTACTGCCCAGTGAGCATTCCGGTTCACTGAAAAGCCTCCCGCTATTGTTCCGCCGCCCGGCATTGATGAGCATTTATTTGTTAACTGTGGTGGTTGTCACCGc                                               >  1:460893/1‑288 (MQ=255)
                                                                                                                                                                                                                                         gggCTCCGGCCTGGAAGCAGGACCAGGCATTGAGTTTAATTGCCACCGGTACAGCACTGGCGATGGTCTTAGGTTTACCTCTCGGGCGCATTGTGGGCCAGAATTTCGGTTGGCGAATGACCTTCTTCGCGATTGGTATTGGGGCGCTTATCACCCTTTTGTGCCTGATTAAGTTACTTCCCTTACTGCCCAGTGAGCATTCCGGTTCACTGAAAAGCCTCCCGCTATTGTTCCGCCGCCCGGCATTGATGAGCATTTATTTGTTAACTGTGGTGGTTGTCACCGCCCa                                            <  2:523872/288‑1 (MQ=255)
                                                                                                                                                                                                                                              ccggccggGAAGCGAGCACAGGCATTGAGTTTAATTGCCACCGGTACGGCACTGGCGATGGTCTTAGGTTTACCTCTCGGGCGCATTGTGGGCCAGTATTTCGGTTGGCGAATGACCTTCTTCGCGATTGGTATTGGGGCGCTTATCACCCTTTTGTGCCTGATTAAGTTACTTCCCTTACTGCCCAGTGAGCATTCCGGTTCACTGAAAAGCCTCCCGCTATTGTTCCGCCGCCCGGCATTGATGAGCATTTATTTGTTAACTGTGGTGGTTGTCACCGCCCATTaca                                       <  2:666304/289‑1 (MQ=255)
                                                                                                                                                                                                                                              ccggccggGAAGCGAGCACAGGCATTGAGTTTAATTGCCACCGGTACAGCACTGGCGATGGTCTTAGGTTTACCTCTCGGGCGCATTGTGGGCCAGTATTTCGGTTGGCGAATGACCTTCTTCGCGATTGGTATTGGGGCGCTTATCACCCTTTTGTGCCTGATTAAGTTACTTCCCTTACTGCCCAGTGAGCATTCCGGTTCACTGAAAAGCCTCCCGCTATTGTTCCGCCGCCCGGCATTGATGAGCATTTATTTGTTAACTGTGGTGGTTGTCACCGCCCATTaca                                       <  2:195252/289‑1 (MQ=255)
                                                                                                                                                                                                                                              ccggccggGAAGCGAGCACAGGCATTGAGTTTAATTGCCACCGGTACAGCACTGGCGATGGTCTTAGGTTTACCTCTCGGGCGCATTGTGGGCCAGTATTTCGGTTGGCGAATGACCTTCTTCGCGATTGGTATTGGGGCGCTTATCACCCTTTTGTGCCTGATTAAGTTACTTCCCTTACTGCCCAGTGAGCATTCCGGTTCACTGAAAAGCCTCCCGCTATTGTTCCGCCGCCCGGCATTGATGAGCATTTATTTGTTAACTGTGGTGGTTGTCACCGCCCATTaca                                       <  1:92087/289‑1 (MQ=255)
                                                                                                                                                                                                                                               cggccggGAAGCGAGCACAGGCATTGAGTTTAATTGCCACCGGTACAGCACTGGCGATGGTCTTAGGTTTACCTCTCGGGCGCATTGTGGGCCAGTATTTCGGTTGGCGAATGACCTTCTTCGCGATTGGTATTGGGGCGCTTATCACCCTTTTGTGCCTGATTAAGTTACTTCCCTTACTGCCCAGTGAGCATTCCGGTTCACTGAAAAGCCTCCCGCTATTGTTCCGCCGCCCGGCATTGATGAGCATTTATTTGTTAACTGTGGTGGTTGTCACCGCCCATTacac                                      >  1:620530/1‑289 (MQ=255)
                                                                                                                                                                                                                                                   cgtgAATCGAGGCCAGGCATTGCGTTGAATTGCCACCGTTACAGCACTGGCGATGGGCTTAGTTTTACCTCTCGGGCGCATTGTGGTCCAGTATTTCGGTTGGCCAATGACCTTCTTCGCGATTGGTATTGGGGCGCTTATCACCCTTTTGTGCCTGATTAAGTTACTTCCCTTACTGCCCAGTGAGCATTCCGATTCACTGAAAAGCCTCCCGCTATTGTTCCGCCGCCCGGCATTGATGAGCATTTATTTGTTAACTGTGGTGGTTGTCACCGCCCATTACACGGCa                                  <  1:483078/286‑1 (MQ=255)
                                                                                                                                                                                                                                                   cggGAAGCGAGCACAGGCATTGAGTTTAATTGCCACCGGTACAGCACTGGCGATGGTCTTAGGTTTACCTCTCGGGCGCATTGTGGGCCAGTATTTCGGTTGGCGAATGACCTTCTTCGCGATTGGTATTGGGGCGCTTATCACCCTTTTGTGCCTGATTAAGTTACTTCCCTTACTGCCCAGTGAGCATTCCGGTTCACTGAAAAGCCTCCCGCTATTGTTCCGCCGCCCGGCATTGATGAGCATTTATTTGTTAACTGTGGTGGTTGTCACCGCCCATTACACGGCa                                  <  2:620530/289‑1 (MQ=255)
                                                                                                                                                                                                                                                   cggGAAGCGAGCACAGGCATTGAGTTTAATTGCCACCGGTACAGCACTGGCGATGGTCTTAGGTTTACCTCTCGGGCGCATTGTGGGCCAGTATTTCGGTTGGCGAATGACCTTCTTCGCGATTGGTATTGGGGCGCTTATCACCCTTTTGTGCCTGATTAAGTTACTTCCCTTACTGCCCAGTGAGCATTCCGGTTCACTGAAAAGCCTCCCGCTATTGTTCCGCCGCCCGGCATTGATGAGCATTTATTTGTTAACTGTGGTGGTTGTCACCGCCCATTACACGGCa                                  <  1:101060/289‑1 (MQ=255)
                                                                                                                                                                                                                                                      gAAGCTAGCACAGGCATTGAGTTTAATTGCCACCGGTACAGCACTGGCGATGGTCTTATGTTTACCTCTCGGGCGCATTGTGGGCCAGTATGTCGGTGGGCGAATGACCTTCTTCGCGATTGGTATTGGGGCGCTTATCACCCTTTTGTGCCTGATTAAGTTACTTCCCTTACTGCCCAGTGAGCATTCCGGTTCACTGAAAAGCCTCCCGCTATTGTTCCGCCGCCCGGCATTGATGAGCATTTATTTGTTAACTGTGGTGGTTGTCACCGCCCATTACACGGCATa                                <  1:570504/288‑1 (MQ=255)
                                                                                                                                                                                                                                                                   gCATTGAGTTTAATTGCCACCGGTACAGCACTGGCGATGGTCTTAGGTTTACCTCTCGGGCGCATTGTGGGCCAGTATTTCGGTTGGCGAATGACCTTCTTCGCGATTGGTATTGGGGCGCTTATCACCCTTTTGTGCCTGATTAAGTTACTTCCCTTACTGCCCAGTGAGCATTCCGGTTCACTGAAAAGCCTCCCGCTATTGTTCCGCCGCCCGGCATTGATGAGCATTTATTTGTTAACTGTGGTGGTTGTCACCGCCCATTACACGGCATACAGCTATATCGAg                   >  1:386794/1‑288 (MQ=255)
                                                                                                                                                                                                                                                                      ttGAGTTTAATTGCCACCGGTACAGCACTGGCGATGGTCTTAGGTTTACCTCTCGGGCGCATTGTGGGCCAGTATTTCGGTTGGCGAATGACCTTCTTCGCGATTGGTATTGGGGCGCTTATCACCCTTTTGTGCCTGATTAAGTTACTTCCCTTACTGCCCAGTGAGCATTCCGGTTCACTGAAAAGCCTCCCGCTATTGTTCCGCCGCCCGGCATTGATGAGCATTTATTTGTTAACTGTGGTGGTTGTCACCGCCCATTACACGGCATACAGCTATATCGAGCCtt               <  2:245206/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                             tAATTGCCACCGGTACAGCACTGGCGATGGTCTTAGGTTTACCTCTCGGGCGCATTGTGGGCCAGTATTTCGGTTGGCGAATGACCTTCTTCGCGATTGGTATTGGGGCGCTTATCACCCTTTTGTGCCTGATTAAGTTACTTCCCTTACTGCCCAGTGAGCATTCCGGTTCACTGAAAAGCCTCCCGCTATTGTTCCGCCGCCCGGCATTGATGAGCATTTATTTGTTAACTGTGGTGGTTGTCACCGCCCATTACACGGCATACAGCTATATCGAGCCTTTTGTACa        >  2:661782/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                                   ccACCGGTACAGCACTGGCGATGGTCTTAGGTTTACCTCTCGGGCGCATTGTGGGCCAGTATTTCGGTTGGCGAATGACCTTCTTCGCGATTGGTATTGGGGCGCTTATCACCCTTTTGTGCCTGATTAAGTTACTTCCCTTACTGCCCAGTGAGCATTCCGGTTCACTGAAAAGCCTCCCGCTATTGTTCCGCCGCCCGGCATTGATGAGCATTTATTTGTTAACTGTGGTGGTTGTCACCGCCCATTACACGGCATACAGCTATATCGAGCCTTTTGTACAAAACAt  >  1:640022/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                                   ccACCGGTACAGCACTGGCGATGGTCTTAGGTTTACCTCTCGGGCGCATTGTGGGCCAGTATTTCGGTTGGCGAAAGACCTTCTTCGCGATTGCTAGTGGGGCGCTTATCACGCTTTTGTGCCTGATTAAGTTACTTCCCTTACTGCCCAGTGAGCATTCCGGTTCCCTGAAAAGCCTCCCGCTATTTTTCCTCCGCCCGGCATTGATGAGCATTTATTTGTTAACTGTGGTGGTTGTCACCGCCCCTTACACGGCATGCAGCTATATCGAGCCTTTGTTACAAAACAt  >  2:110778/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                                                      
CATCATGTTGACCATTTACGCATGGGTAGTAGCGCTAATGTCATTGCCTTTTATGTTAATGACCAGTCAGGTTGAACGGCGCAAATTACTGATCTGCCTGTTTGTGGTGTTTATTGCCAGCCACGTACTGTCGTTTTTGTCGTGGAGCTTTACCGTTCTGGTGATCAGTCGCATTGGTGTGGCTTTTGCACATGCGATTTTCTGGTCGATTACGGCGTCTCTGGCGATCCGTATGGCTCCGGCCGGGAAGCGAGCACAGGCATTGAGTTTAATTGCCACCGGTACAGCACTGGCGATGGTCTTAGGTTTACCTCTCGGGCGCATTGTGGGCCAGTATTTCGGTTGGCGAATGACCTTCTTCGCGATTGGTATTGGGGCGCTTATCACCCTTTTGTGCCTGATTAAGTTACTTCCCTTACTGCCCAGTGAGCATTCCGGTTCACTGAAAAGCCTCCCGCTATTGTTCCGCCGCCCGGCATTGATGAGCATTTATTTGTTAACTGTGGTGGTTGTCACCGCCCATTACACGGCATACAGCTATATCGAGCCTTTTGTACAAAACAT  >  NC_000913/1617180‑1617743

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: