Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,590,669 G→T 8.3% L1025L (CTG→CTT acrD → aminoglycoside/multidrug efflux system

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,590,6690GT 8.3% 134.9 / 2.3 48L1025L (CTG→CTTacrDaminoglycoside/multidrug efflux system
Reads supporting (aligned to +/- strand):  ref base G (22/22);  new base T (2/2);  total (24/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.80e-01

TTGCTAACGAGATGAACCAAAAAGGCCACGACCTGTTTGAAGCGACGCTCCACGCCTGCCGTCAGCGTTTACGCCCGATTCTGATGACCTCGCTGGCATTTATCTTCGGCGTATTGCCAATGGCAACCAGCACGGGTGCCGGTTCCGGTGGTCAGCATGCGGTGGGTACTGGCGTAATGGGCGGGATGATTTCGGCCACTATTCTGGCTATTTACTTCGTGCCGCTGTTCTTTGTGCTGGTGCGCCGCCGCTTCCCGCTGAAGCCGCGCCCGGAATAAGCAATAAAAAAGGCGACATGCCAATGTGTCGCCTTTTTCAACTTTCCGATTAAGAACCTGCTCAGCGGGTTCTTGCTGTTTGTACTTTGTCTCAGGAATTACTTACGAAGCATAACTTCGATAAAGTCTTTCCAGTTCCCCAGTTCACGTTCAATCATAACAACCTCTCTTATAATTATGGGTATTCTACGGAAACAATATACCGTGGTGAAGCTAATTTACTCGATTGCTGCG  >  NC_000913/2590431‑2590942
                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                                                 
ttGCTAACGAGATGAACCAAAAAGGCCACGACCTGTTTGAAGCGACGCTCCACGCCTGCCGTCAGCGTTTACGCCCGATTCTGATGACCTCGCTGGCATTTATCTTCGGCGTATTGCCAATGGCAACCAGCACGGGTGCCGGTTCCGGTGGTCAGGATGCGGTGGGTAGTGGCGTAATGGGCGGGATGATTTCGGCCACTATTCTGGCTATTTACTTCGTGCCGCTGTTCTTTGTGCTGGTGCGCCGCCGATTCCCGCTGAAGCCGCGCCCggca                                                                                                                                                                                                                                               >  1:99163/1‑273 (MQ=255)
 tGCTAACGAGATGAACCAAAAAGGCCACGACCTGTTTGAAGCGACGCTCCACGCCTGCCGTCAGCGTTTACGCCCGATTCTGATGACCTCGCTGGCATTTATCTTCGGCGTATTGCCAATGGCAACCAGCACGGGTGCCGGTTCCGGTGGTCAGCATGCGGTGGGTACTGGCGTAATGGGCGGGATGATTTCGGCCACTATTCTGGCTATTTACTTCGTGCCGCTGTTCTTTGTGCTGGTGCGCCGCCGCTTCCCGCTGAAGCCGCGCCGGGAAt                                                                                                                                                                                                                                              >  1:18550/1‑275 (MQ=255)
                  aaaaaGGCCACGACCTGTTTGAAGCGACGCTCCACGCCTGCCGTCAGCGTTTACGCCCGATTCTGATGACCTCGCTGGCATTTATCTTCGGCGTATTGCCAATGGCAACCAGCACGGGTGCCGGTTCCGGTGGTCAGCATGCGGTGGGTACTGGCGTAATGGGCGGGATGATTTCGGCCACTATTCTGGCTATTTACTTCGTGCCGCTGTTCTTTGTGCTGGTGCGCCGCCGCTTCCCGCTGa                                                                                                                                                                                                                                                             >  2:259745/1‑243 (MQ=255)
                          cACGACCTGTTTGAAGCGACGCTCCACGACTGCCGTCAGCGTTTACGCGCGATTCTGAAGACCTCGCTGGCATTTATCTTCGGCGTATTGCCAATGGCAACCAGCACGGGTGCCGGTTCCGGCGGTCACCATGCGGAGGGTACTGGCAAACTGGGCGGGATGACTTCGGCAACCATTATGTCTATTTACTTCGTCCCGCCGTTCTTTGTGTTGGTTCGCCCCCGCTTCCTGCGGAAGCCAGGCCCGACATAAGCAATAAAAAAGGCGAAATACCa                                                                                                                                                                                                                     >  2:79085/1‑275 (MQ=255)
                           aCGACCTGTTTGAAGCGACGCTCCACGCCTGCCGTCAGCGTTTACGCCCGATTCTGATGACCTCGCTGGCATTTATCTTCGGCGTATTGCCAATGGCAACCAGCACGGGTGCCGGTTCCGGTGGTCAGCATGCGGTGGGTACTGGCGTAGTGGGCGGGATGATTTCGGCCACTATTCTGGCTATTTACTTCGTGCCGCTGTTCTTTGTGCTGGTGCGCCGCCGCTTCCCGCTGAAGCCGCGCCCGGAATAAGCAATAAAAAAGGCGACATGCCa                                                                                                                                                                                                                     >  2:163532/1‑274 (MQ=255)
                               ccTGTTTGAGGCGACGCTCCACGCCTGGCGTCAGCGTTTACGCCCGATTCTGATGACCTCGCTGGCATTTATCTTCGGCGTATTGCCAATGGCAACCAGCACGGGTGCCGGTTCCGGTGGTCAGCATCCGGTGGGTACTGGCGTAATGGGCGGGCTGATTTCGGCGACTATTCTTGCTATTTACTTCGTGCCGCTGTTCTTTGTGCTGGTGCGCCGCCGCTTCCCGCTGAAGCCGCGCCCGGAATAAGCAATAAAAAAGGCGACATGCCAAtgtg                                                                                                                                                                                                                <  1:261294/275‑1 (MQ=255)
                                  gTTTGAAGCGACGCTCCACGCCTGCCGTCAGCGTTTACGCCCGATTCTGATGACCTCGCTGGCATTTATCTTCGGCGTATTGCCAATGGCAACCAGCACGGGTGCCGGTTCCGGTGGTCAGCATGCGGTGGGTACTGGCGTAAGGGGCGGGATGATTTCGGTCACTATTCTGGCTATTTACTTCGTGCCGCTGTTTTTTGTGCTGGTGCGCCGCCGCTTCCCGCTGAAGCCGCGCCGGGAATAAGCAACAAAAAAGCCGCAATGCCAATGTGTc                                                                                                                                                                                                              >  2:64490/1‑274 (MQ=255)
                                      ggagggACGCCCCCCGCCGCCCTTCAGCGTTTACGCCCGATTCTGATGCCCTCGCTTGCATTTATCTTCGGCGTATTGCCAATGGCAACCAGCACGGGTGCCGGTTCCGGTGGTCAGCATGCGGTGGGTACTGGCGTAATGGGCTGGATGATTTCGGCCACTATTCTGGCTATTTACTTCGTGCCGCTGTTCTTTGTGCTGGTGCGCCGCCGCTTCCCGCTGAAGCCGCGCCCGGAATAAGCAATAAAAAAGGCGACATGCCAATGTGTCGCCtt                                                                                                                                                                                                         <  1:23185/270‑1 (MQ=255)
                                              gCTCCACGGCTGCCTTCAGCGTTTACGCCCGATTCTGATGACCTCGCTGGCATTTATCTTCGGCGTATTGCCAATGGCAACCAGCACGGGTGCCGGTTCCGGTGGTCAGCATGCGGTGGGTACTGGCGTACTGGGCGGGATGATTTCGGCCACTATTCTGGCTATTTACTTCGTGCCGCTGTTCTTTGTGCTGGTGCGCCGCCGCTTCCCGCTGAAGCCGCGCCCGGAATAAGCAATAAAAAAGGCGACATGCCAATGTGTCGCCTTTTTCAACt                                                                                                                                                                                                 <  1:64490/275‑1 (MQ=255)
                                                          ccGTCAGCGTTTACGCCCGATTCTGATGACCTCGCTGGCATTTATCTTCGGCGTATTGCCAATGGCAACCAGCACGGGTGCCGGTTCCGGTGGTCAGCATGCGGTGGGTACTGGCGTACTCGGCGGGATGATTTCGGCCACTTTTCTGGCTATTTACTTCGTGCCGCTGTTCTTTGTGCTTGCTCGCCGCCGCTTCCCGCTGCAGCCGCCCCCGGAATAAGCAATAAAAAAGGCGACATGCCACTGTGTCCCCTTTTTCAATTTTCCGTTTAAgc                                                                                                                                                                                     >  2:335642/1‑274 (MQ=255)
                                                          ccGTCAGCGTTTACGCCCGATTCTGATGACCTCGCTGGCATTTATCTTCGGCGTATTGCCAATGGCAACCAGCACGGGTGCCGGTTCCGGTGGTCAGCATGCGGTGGGTACTGGCGTAATGGGCGGGATGCTTACGGCCACTATTCTGGCTATTTACTTCGTGCCGCTGTTCTTTGTGCTGGTTCGACGCCGCTTCCCGCTGAAGCCGCGCCCGGAATAAGCAATAAAAAAGGCGACATGCCAATGTGTAGCCTTTTTCAACTTTCCGATTaaaa                                                                                                                                                                                     >  1:196409/1‑273 (MQ=255)
                                                          ccGTCAGCGTTTACGCCCGATTCTGATGACCTCGCTGGCATTTATCTTCGGCGTATTGCCAATGGCAACCAGCACGGGTGCCGGTTCCGGTGGTCAGCATGCGGTGGGTACTGGCGTAATGGGCGGGATGATTTCGGCCACTATTCTGGCTATTTACTTCGTGCCGCTGTTCTTTGTGCTGGTGCGCCGCCGCTTCCCGCTGAAGCCGCGCCCGGAATAAGGAATAAAAAAGGCCACATTCCAACGTTTGCCCTTTCTACACTTTCCGATTaaaa                                                                                                                                                                                     >  2:204277/1‑273 (MQ=255)
                                                                     tACGCCCGTTTCTGATGACCCTGTTGGCAGTAATCTTCGGAGTATGGCCAATGGCAACCAGCACGGGTGCCGGTTCCGGTGGTCAGCATGCGGTGGGTACTGGCGTAATGGGCTGGATGATTTCGGCCACTATTCTGGCTATTTACTTCGTGCCGCTGTTCTTTGTGCTGGTGCGCCGCCGCTTCCCGCTGAAGCCGCGCCCGGAATAAGCAATAAAAAAGGCGACATGCCAATGTGTCGCCTTTTTCAACTTTCCGATTAAGAACCTGCTCAGc                                                                                                                                                                          <  2:99163/275‑1 (MQ=255)
                                                                     tACGCCCGATTCTGATGACCTCGCTGGCATTTATCTTCGGCGTATTGCCAATGGCAACCAGCACGGGTGCCGGTTCCGGTGGTCAGCATGCGGTGGGTACTGGCGTAATGGGCGGGATGATTTCGGCCACTATTCTGGCTATTTACTTCGTGCCGCTGTTCTTTGTGCTGGTGCGCCGCCGCTTCCCGCTGAAGCCGCGCCCGGAATAAGCAATAAAAAAGGCGACATGCCAATGTGTCGCCTTTTTCAACTTTCCGATTAAGAACCTGCTCAGc                                                                                                                                                                          >  1:258015/1‑275 (MQ=255)
                                                                                cTGATGACCTCGCTGGCATTTATCTTCGGCGTATTGCCAATGGCAACCAGCACGGGTGCCGGTTCCGGTGGTCAGCATGCGGTGGGTACTGGCGTAATGGGCGGGATGATTTCGGCCACTATTCTGGCTATTTACTTCGTGCCGCTGTTCTTTGTGCTGGTGCGCCGCCGCTTCCCGCTGAAGCCGCGCCCGGAATAAGCAATAAAAAAGGCGACATGCCAATGTGTCGCCTTTTTCAACTTTCCGATTAAGAACCTGCTCAGCGGGTTCtt                                                                                                                                                                  <  1:335642/272‑1 (MQ=255)
                                                                                   atgaCCTCGCTGGCATTTATCTTCGGCGTATTGCCAATGGCAACCAGCACGGGTGCCGGTTCCGGTGGTCAGCATGCGGTGGGTACTGGCGTAATGGGCGGGATGATTTCGGCCACTATTCTGGCTATTTACTTCGTGCCGCTGTTCTTTGTGCTGGTGCGCCGCCGCTTCCCGCTGAAGCCGCGCCCGGAATAAGCAATAAAAAAGGCGACATGCCAATGTGTCGCCTTTTTCAACTTTCCGATTAAGAACCTGCTCAGCGGGTTCTTGCTGtt                                                                                                                                                            <  1:32467/275‑1 (MQ=255)
                                                                                   atgaCCTCGATGGCATTTATCTTCGGCGTATTGCCACTGGCAACCCGCCCGGGTGCCGGTTCCGGTGGTCAGCATGCGGTGGGTACTGGCGTAATGGGCGGGATGATTTCGGCCTCTATCCTGGCTATTTACTTCGTGCCGCTGTTCTTTGTGCTGGTGCGCCGCCGCTTCCCGCTGAAGCCCCTCCAGGCATAAGCAATAAAAAAGGCGACATGCCAATCTGTCGCCTTTTCCCACTCTCCGATTAAGAATCCGCCCAGCGGGTTCTTGCTGtt                                                                                                                                                            >  1:28040/1‑275 (MQ=255)
                                                                                          cGCTGGCATTTATCTTCGGCGTATTGCCAATGGCAACCAGCACGGGTGCCGGTTCCGGTGGTCAGCATGCGGTGGGTACTGGCGTAATGGGCGGGATGATTTCGGCCACTATTATGGCTATTTACTTCGTGCCGCTGTTCTTTGTGCTGGTGCGCCGCCGCTTCCCGCTGAAGCCGCGCCCGGAATAAGCAATAAAAAAGGCGACATGCCAATGTGTCGCCTTTTTCAACTTTCCGATTACGACACTTCTCAGCGGGTTCGTGCTGTTTGCACtt                                                                                                                                                     >  2:333907/1‑275 (MQ=255)
                                                                                           gCTGGCATTTATCTTCGGCGTATTGCCAATGACAACCAGCACGGGTGCCGGTTCCGGTGGTCAGCATGAGGAGGGAACTGGCGTCATGGGCGGGATGATTTCGGTCACTATTATGGCTATTTACTTCGTTCCGCTGTTCTTTGTGCTGGTGCGCAGCCGCTTCCCGTTGACGCAGCGCCCGGAATAAGCAATAAAAAAGGCGACATGCCAACGTGTCGCCTTTTTCAACTTTCCGATTAAGCAACTGCTCAGCGGGTTCCTGCTGTTTGttattt                                                                                                                                                    >  2:388255/1‑270 (MQ=255)
                                                                                              ggCATTTTCCTTGGGCGTATGGCCAATGGCAACCAGGACGGGTGCCGGTGCCGGTGGTAAGCATGCGGGGGGTACTGGCGTAATGGGCGGGGTTATTTCGGCCACTATTCTGGCTATTTACTTCGTGCCGCTGTTCTTTGTGCTGGTGCGCCGCCGCTTCCCGCTGAAGCCGCGCCCGGAATAAGCAATAAAAAAGGCGACATGCCAATGTGTCGCCTTTTTCAACTTTCCGATTAAGAACCTGCTCAGCGGGTTCTTGCTGTTTGTACTTTGtc                                                                                                                                                 <  2:28040/275‑1 (MQ=255)
                                                                                              ggCATTTATCTTCGGCGTATTGCCAATGGCAACCAGCACGGGTGCCGGTTCCGGTGGTCAGCATGCGGTGGGTACTGGCGTAATGGGCGGGATGATTTCGGCCACTATTCTGGCTATTTACTTCGTGCCGCTGTTCTTTGTGCTGGTGCGCCGCCGCTTCCCGCTGAAGCCGCGCCCGGAATAAGCAATAAAAAAGGCGACATGCCAATGTGTCGCCTTTTTCAACTTTCCGATTAAGAACCTGCTCAGCGGGTTCTTGCTGTTTGTACTTTGtc                                                                                                                                                 <  2:258015/275‑1 (MQ=255)
                                                                                               gCATTTAGCTTCGGCGTATTGCCAATGGCAACCAGGACGGGTGCCGGTTCCGGTGGTCAGCATGCGGTGGGTACTGGCGTAATGGGCGGGATGATTTCGGCCACTATTCTGGCTATTTACTTCGTGCCGCGGTTCTTTGTGCTGGTGCGCCGCCGCTTCCCGCTGAAGCCGCGCCCGGAATAAGCAATAAAAAAGGCGACATGCCAATGTGTCGCCTTTTTCAACTTTCCGATTAAGAACCTGCTCAGCGGGTTCTTGCTGTTTGTACTTTGtct                                                                                                                                                <  2:89504/275‑1 (MQ=255)
                                                                                                                    ccgaggggaaCCAGGAAGGGGGCCGGTTCGCGGTGTTAGCATGCGGTGGTTGCTGGCGGAATGGGAGGGATGATTTCGGCCACTATTCTTGGTATTGACTTCGTGCCGCTGTTCTTTGGGGTGGTTGGCCGCCGCGTCCCGCTGAAGCCGCGCCCGGAATAGGCAATAAAAAAGGCGCCTTGCCAATGTGTCGCCTTTTTCAACTTTCCGATTAAGAACCCGCTCAGCCGGTTCTTGCTGTTTGTACTCTGTCGCAGGAATTACTTACGACGCAt                                                                                                                           <  2:211271/267‑1 (MQ=255)
                                                                                                                        tGGCAACCAGCACGGGTGCCGGTTCCGGTGGTCAGCATGCGGTGGGTACTGGCGTAAGGGGCGGGATGATTTCGGCCACTATTCTGGCTATTTACTTCGTGCCGCTGTTCTTTGTGCTGGTGCGCCGCCGCTTCCCGCTGAAGCCGCGCCCGGAATAAGCAATAAAAAAGGCGACATGCCAATGTGTCGCCTTTTTCAACTTTCCGATTAAGAACCTGCTCAGCGGGTTCTTGCTGTTTGTACTTTGTCTCAGGAATTACTTACGAAGCATAACt                                                                                                                       <  1:204277/275‑1 (MQ=255)
                                                                                                                              ccAGCACGGGTGCCGGTTCCGGTGGTCAGCATGCGGTGGGTACTGGCGTAATGGGCGGGATGATTTCGGCCACTATTCTGGCTATTTACTTCGTGCCGCTGTTCTTTGTGCTGGTGCGCCGCCGCTTCCCGCTGAAGCCGCGCCCGGAATAAGCAATAAAAAAGGCGACATGCCAATGTGTCGCCTTTTTCAACTTTCCGATTAAGAACCTGCTCAGCGGGTTCTTGCTGTTTGTACTTTGTCTCAGGAATTACTTACGAAGCATAACTTCGAt                                                                                                                  >  1:46860/1‑274 (MQ=255)
                                                                                                                                                                 ggTGGGTACTGGCGTAATGGGCGGGATGATTTCGGCCACTATTCTGGCTATTTACTTCGTGCCGCTGTTCTTTGTGCTGGTGCGCCGCCGCTTCCCGCTGAAGCCGCGCCCGGAATAAGCAATAAAAAAGGCGACATGCCAATGTGTCGCCTTTTTCAACTTTCCGATTAAGAACCTGCTCAGCGGGTTCTTGCTGTTTGTCCTTTGTCTCAGGAATTACTTACGAAGCATAACTTCGATAAAGTCttt                                                                                                         >  1:304370/1‑249 (MQ=255)
                                                                                                                                                                     ggTACTGGCGTAATGGGCGGGATGATTTCGGCCACTATTCTGGCTATTTACTTCGTGCCGCTGTTCTTTGTGCTGGTGCGCCGCCGCTTCCCGCTGAAGCCGCGCCCGGAATAAGCAATAAAAAAGGCGACATGCCAATGTGTCGCCTTTTTCAACTTTCCGATTAAGAACCTGCTCAGCGGGTTCTTGCTGTTTGTACTTTGTCTCAGGAATTACTTACGAAGCATAACTTCGATAAAGTCTTTCCAg                                                                                                     <  2:304370/249‑1 (MQ=255)
                                                                                                                                                                         cTGGCGGAATGGGCGGGATGATTTCGGCCACTATTCTGGCTATTTACTTCGTGCCGCTGTTCTTTGTGCTGGTGCGCCGCCGCTTCCCGCTGAAGCCGCGCCCGGAATAAGCAATAAAAAAGGCGACATGCCAATGTGTCGCCTTTTTCAACTTTCCGATTAAGAACCTGCTCAGCGGGTTCTTGCTGTTTGTACTTTGTCTCAGGAATTACTTACGAAGCATAACTTCGATAAAGTCTTTCCAGTTCCCCAGTTCACGTTCAATCATaacaac                                                                        <  1:16265/274‑1 (MQ=255)
                                                                                                                                                                         cTGGCGGAATGGGCGGGATGATTTCGGCCACTATTCTGGCTATTTACTTCGTGCCGCTGTTCTTTGTGCTGGTGCGCCGCCGCTTCCCGCTGAAGCCGCGCCCGGAATAAGCAATAAAAAAGGCGACATGCCAATGTGTCGCCTTTTTCAACTTTCCGATTAAGAACCTGCTCAGCGGGTTCTTGCTGTTTGTACTTTGTCTCAGGAATTACTTACGAAGCATAACTTCGATAAAGTCTTTCCAGTTCCCCAGTTCACGTTCAATCATAACAAcc                                                                       <  1:333907/275‑1 (MQ=255)
                                                                                                                                                                           gtcGTAATGGGCGGGATGATTTCGTCCACTATTCTGGCTATTTACTTCGTGCCGCTGTTCTTTGTGCTGGTGCGCCGCCGCTTCCCGCTGAAGCCGCGCCCGGAATAAGCAATAAAAAAGGCGACATGCCAATGTGTCGCCTTTTTCAACTTTCCGATTAAGAACCTGCTCAGCGGGTTCTTGCTGTTTGTACTTTGTCTCAGGAATTACTTACGAAGCATAACTTCGATAAAGTCTTTCCAGTTCCCCAGTTCACGTTCAATCATaacaac                                                                        <  1:323787/270‑1 (MQ=255)
                                                                                                                                                                           ggCGTAATGGGCGGGATGATTTCGGCCACTATTCTGGCTATTTTCTTCGGGCCGCTGTTCTTTGGGCTGGTGCGCCGCCGCTTCCCGCTGAAGCCGCGCCCGGAATAAGCAATAAAAAAGGCGACATGCCAATGTGTCGCCTTTTTCAACTTTCCGATTAAGAACCTGCTCAGCGGGTTCTTGCTGTTTGTACTTTGTCTCAGGAATTACTTACGAAGCATAACTTCGATAAAGTCTTTCCAGTTCCCCAGTTCACGTTCAATCATaacaac                                                                        <  2:196409/272‑1 (MQ=255)
                                                                                                                                                                               tAATGGGCGGGATGATTTCGGCCACTATTCTGGCTATTTACTTCGTGCCGCTGTTCTTTGTGCTTGTGCGCCGCCGCTTCCCGCTGAAGCCGCGCCCGGAATAAGCAATAAAAAAGGCGACATGCCAATGTGTCGCCTTTTTCAACTTTCCGATTAAGAACCTGCTCAGCGGGTTCTTGCTGTTTg                                                                                                                                                          >  1:289209/1‑186 (MQ=255)
                                                                                                                                                                                 aTGGGCGGGATGATTTCGGCCACTATTCTGGCTATTTACTTCGTGCCGCTGTTCTTTGTGCTGGTGCGCCGCCGCTTCCCGCTGAAGCCGCGCCCGGAATAAGCAATAAAAAAGGCGACATGCCAATGTGTCGCCTTTTTCCACTTTCCGATTAAGAACCTGCTCAGCGGGTTCTTGCTGTTTGTACTTTGTCTCAGGAATTACTTACGAAGCATAACTTCGATAAAGTCTTTCCAGTTCCCCAGTTCACGTTCAATCATAACAACCTCTCtt                                                                 >  2:8396/1‑273 (MQ=255)
                                                                                                                                                                                 aTGGGCGGGATGATTTCGGCCACTATTCTGGCTATTTACTCCGTTCCGGTGTTCTTTTTGCTTGTGCGGCGCCGCTTCCCGCTGAAGCCGCGCCCGGAATAAGCAATAAAAAAGGCGACATGCCAATGTGTCGCCTTTTTCAACGTTCCGATTAAGAACCTGCTCAGCGGGTTCTTGCTGTTTGTACTTTGTCTCAGGAATTACTTACGAAGCATAACTTCGATAAAGTCTTTCCAGTTCCCCAGTTCACGTTCAATCATAACAACCTCTCTta                                                                <  1:388255/274‑1 (MQ=255)
                                                                                                                                                                                   gggCGGGATGATTTCGGCCACTATTCTGGCTATTTACTTCGTGCCGCTGTTCTTTGTGCTTGTGCGCCGCCGCTTCCCGCTGAAGCCGCGCCCGGAATAAGCAATAAAAAAGGCGACATGCCAATGTGTCGCCTTTTTCAACTTTCCGATTAAGAACCTGCTCAGCGGGTTCTTGCTGTTTGTACt                                                                                                                                                      <  2:289209/186‑1 (MQ=255)
                                                                                                                                                                                                    ccACTATTCTGGCTGTTTACTTGGTGCCGCTGTTCTTTGGGCTGGTGCGCGACCGCTTCCCGCTGAAGCCGCGCCCGGAATAAGCAATAAAAAAGGCGACATGCCAATGTGTCGCCTTTTCAACTTTCCGATTAAGAACCTGCTCAGCGGGTTCTTGCTGTTTGTACTTTGTCTCAGGAATTACTTACGAAGCATAACTTCGATAAAGTCTTTCCAGTTCCCCAGTTCACGTTCAATCATAACAACCTCTCTTATAATTATGGGTATTCTACgg                                            <  2:18550/274‑1 (MQ=255)
                                                                                                                                                                                                     cACTATTCTGGCTATTTACTTCGTGCCGCTGTTCTTTGTGCTGGTGCGCCGCCGCTTCCCGCTGAAGCCGCGCCCGGAATAAGCAATAAAAAAGGCGACATGCCAATGTGTCGCCTTTTTCAACTTTCCGATTAAGAACCTGCTCAGCGGGTTCTTGCTGTTTGTACTTTGTCTCAGGAATTACTTACGAAGCATAACTTCGATAAAGTCTTTCCAGTTCCCCAGTTCACGTTCAATCATAACAACCTCTCTTATAATTATGGGTATTCTACgg                                            <  2:46860/274‑1 (MQ=255)
                                                                                                                                                                                                       cTATTCTGGCTCTTTACTTCGTGCCGCTGTTCTTTGTGCTGGTGCGCCGCCGCTTCACGCTGAAGCCGCGCCCGGAATAAGCAATAAAAAAGGCGACATGCCAATGTGTCGCCTTTTTCAACATTCCGCTTAAGAACATGCTCAGCGGGTTCTTGCTGTTTGTACTTTGTCCCAGGAATTACTTACGAAGCATAACTTCGATAAATTCTTTCCAGTTCCCCAGTTAACGTTCAATCATAACAACCTTTCTTTAAATTATGGGTTTTCTCCGGa                                           >  2:110845/1‑273 (MQ=255)
                                                                                                                                                                                                         aTTCTGGCTATTTACTTCGTGCCGCTGTTCTTTGTGCTGGTGCGCCGCCGCTTCCCGCTGAAGCCGCGCCCGGAATAAGCAATAAAAAAGGCGACATGCCAATGTGTCGCCTTTTTCAACTTTCCGATTAAGAACCTGCTCAGCGGGTTCTTGCTGTTTGTACTTTGTCTCAGGAATTACTTACGAAGCATAACTTCGATAAAGTCTTTCCAGTTCCCCAGTTCACGTTCAATCATAACAACCTCTCTTATAATTATGGGTATTCTACGGAAACa                                       >  2:95502/1‑275 (MQ=255)
                                                                                                                                                                                                               gCTATTTACTTCGTGCCGCTGTTCTTTGTGCTGGTGCGCCGCCGCTTCCCGCTGAAGCCGCGCCCGGAATAAGCAATAAAAAAGGCGACATGCCAATGTGTCGCCTTTTTCAACTTTCCGATTaa                                                                                                                                                                                       >  2:146758/1‑125 (MQ=255)
                                                                                                                                                                                                                  aTTTACTTCGTGCCGCTGTTCTTTGTGCTGGTGCGCCGCCGCTTCCCGCTGAAGCCGCGCCCGGAATAAGCAATAAAAAAGGCGACATGCCAATGTGTCGCCTTTTTCAACTTTCCGATTAAGAACCTGCTCAGCGGGTTCTTGCTGTTTGTACTTTGTCTCAGGAATTACTTACGAAGCATAACTTCGATAAAGTCTTTCCAGTTCCCCAGtt                                                                                           >  2:136760/1‑214 (MQ=255)
                                                                                                                                                                                                                   tttACTTCGTGCCGCTGTTCTTTGTGCTGGTGCGCCGCCGCTTCCCGCTGAAGCCGCGCCCGGAATAAGCAATAAAAAAGGCGACATGCCAATGTGTCGCCTTTTTCAACTTTCCGATTAAGAAc                                                                                                                                                                                   <  1:146758/125‑1 (MQ=255)
                                                                                                                                                                                                                    ttACTTCGTGCCGCTGTTCTTTGTGCTGGTGCGCCGCCGCTTCCCGCTGAAGCCGCGCCCGGAATAAGCAATAAAAAAGGCGACATGCCAATGTGTCGCCTTTTTCAACTTTCCGATTAAGAACCTGCTCAGCGGGTTCTTGCTGTTTGTACTTTGTCTCAGGAATTACTTACGAAGCATAACTTCGATAAAGTCTTTCCAGTTCCCCAGTTCACGTTCAATCATAACAACCTCTCTTATAATTATGGGTATTCTACGGAAACAATATACCgt                              >  1:308890/1‑273 (MQ=255)
                                                                                                                                                                                                                      aCTTCGTGCCGCTGTTCTTTGTGCTGGTGCGCCGCCGCTTCCCGCTGAAGCCGCGCCCGGAATAAGCAATAAAAAAGGCGACATGCCAATGTGTCGCCTTTTTCAACTTTCCGATTAAGAACCTGCTCAGCGGGTTCTTGCTGTTTGTACTTTGTCTCAGGAATTACTTACGAAGCATAACTTCGATAAAGTCTTTCCAGTTCCCCAGTTCACg                                                                                       <  1:136760/214‑1 (MQ=255)
                                                                                                                                                                                                                         tCGTGCCGCTGTTCTTTGTGCTGGTTCGCCGCCGCTTCCCGCTGAAGCCGCGCCCGGAATAAGCAATAAAAAAGGCGACATGCCAATGTGTCGCCTTTTTCAACTTTCCGATTAAGAACCTGCTCAGCGGGTTCTTGCTGTTTGTACTTTGTCTCAGGAATTACTTACGAAGCATAACTTCGATAAAGTCTTTCCAGTTCCCCAGTTCACGTTCAATCATAACAACCTCTCTTATAATTATGGGTATTCTACGGAAACAATAAACAGTGGTAAAg                       >  2:256142/1‑275 (MQ=255)
                                                                                                                                                                                                                           gTGCCGCTGTTCTTTGTGCTGGTGCGCCGCCGCTTCCCGCTGAAGCCGCGCCCGGAATAAGCAATAAAAAAGGCGACATGCCAATGTGTCGCCTTTTTCAACTTTCCGATTAAGAACCTGCTCAGCGGGTTCTTGCTGTTTGTACTTTGTCTCAGGAATTACTTACGAAGCATAACTTCGATAAAGTCTTTCCAGTTCCCCAGTTCACGTTCAATCATaac                                                                           >  2:343552/1‑221 (MQ=255)
                                                                                                                                                                                                                               cGCTGTTCTTTGTGCTGGTGCGCCGCCGCTTCCCGCTGAAGCCGCGCCCGGAATAAGCAATAAAAAAGGCGACATGCCAATGTGTCGCCTTTTTCAACTTTCCGATTAAGAACCTGCTCAGCGGGTTCTTGCTGTTTGTACTTTGTCTCAGGAATTACTTACGAAGCATAACTTCGATAAAGTCTTTCCAGTTCCCCAGTTCACGTTCAATCATAACAAcc                                                                       <  1:343552/221‑1 (MQ=255)
                                                                                                                                                                                                                                              tGGTGCGCCGCCGCTTCCCGCTGAAGCCGCGCCCGGAATAAGCAATAAAAAAGGCGACATGCCAATGTGTCGCCTTTTTCAACTTTCCGATTAAGAACCTGCTCAGCGGGTTCTTGCTGTTTGTACTTTGTCTCAGGAATTACTTACGAAGCATAACTTCGATAAAGTCTTTCCAGTTCCCCAGTTCACGTTCAATCATAACAACCTCTCTTATAATTATGGGTATTCTACGGAAACAATATACCGTGGTGAAGCTAATTTACTCGATTGCTGCg  <  1:163532/275‑1 (MQ=255)
                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                                                 
TTGCTAACGAGATGAACCAAAAAGGCCACGACCTGTTTGAAGCGACGCTCCACGCCTGCCGTCAGCGTTTACGCCCGATTCTGATGACCTCGCTGGCATTTATCTTCGGCGTATTGCCAATGGCAACCAGCACGGGTGCCGGTTCCGGTGGTCAGCATGCGGTGGGTACTGGCGTAATGGGCGGGATGATTTCGGCCACTATTCTGGCTATTTACTTCGTGCCGCTGTTCTTTGTGCTGGTGCGCCGCCGCTTCCCGCTGAAGCCGCGCCCGGAATAAGCAATAAAAAAGGCGACATGCCAATGTGTCGCCTTTTTCAACTTTCCGATTAAGAACCTGCTCAGCGGGTTCTTGCTGTTTGTACTTTGTCTCAGGAATTACTTACGAAGCATAACTTCGATAAAGTCTTTCCAGTTCCCCAGTTCACGTTCAATCATAACAACCTCTCTTATAATTATGGGTATTCTACGGAAACAATATACCGTGGTGAAGCTAATTTACTCGATTGCTGCG  >  NC_000913/2590431‑2590942

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: