Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,296,268 T→C 26.5% intergenic (+474/+168) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,296,2680TC26.5% 83.0 / 24.5 45intergenic (+474/+168)gltP/yjcOglutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
Reads supporting (aligned to +/- strand):  ref base T (19/14);  new base C (9/3);  total (28/17)
Fisher's exact test for biased strand distribution p-value = 4.88e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01

CAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGC‑CTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTA‑TGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTA‑‑CCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCA  >  NC_000913/4295987‑4296550
                                                                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                                                            
cagaaggcggAATTGCCTGATTGTGCGTGGTTTGGTCGGTCGGATAAGGCGTTTACGCCGCATCCGCCATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGC‑CTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTA‑CGCCGCa                                                                                                                                                                                                                                                                                        <  2:621018/282‑1 (MQ=11)
  gACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACTGCGCAATAGC‑CTGCCTTCGCGTGATTTTGTAGTTCCGATAAGGCGATTA‑CGCCGCATc                                                                                                                                                                                                                                                                                      >  2:427021/1‑288 (MQ=255)
       cgcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCCACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGC‑CTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTA‑CGCCGCATCCGACa                                                                                                                                                                                                                                                                                 >  2:204092/1‑288 (MQ=25)
       cgcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGC‑CTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTA‑CGCCGCATCCGACa                                                                                                                                                                                                                                                                                 >  1:252673/1‑288 (MQ=25)
                 tGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATACGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTCATGCCGCATACGACATCAACGCCTGATGCGCCGCTTACCGCTTCTTATCAGGCCTATACCAGCCAGCGCACTAGCACTCA‑TTCGCGTGCTTTTGTATGTCGGATAAGGCGTTTA‑CGACGTATCCGAAAtgatgcccac                                                                                                                                                                                                                                                                       >  2:182836/1‑279 (MQ=11)
                  gATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGC‑CTGATTTAGCGTGATTTTGTAGGTCGGATAAGTCGTTTA‑CGACGCATTCCACCTCCATCCCTGAt                                                                                                                                                                                                                                                                     >  2:154035/1‑289 (MQ=255)
                  gATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGC‑CTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTA‑CGCCGCATCCGACATCAATGCCTGAt                                                                                                                                                                                                                                                                     >  1:406825/1‑289 (MQ=21)
                   aTTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTACCGAGTCTTATCAGGCCTACGCCAGACCGCGGAATACC‑CTGATTTCGGGTGATCTTGTAGGTCGGATAATGTGTTTACTCCCGCTTCCAACAT‑AATGCCTGact                                                                                                                                                                                                                                                                    >  2:454840/1‑287 (MQ=11)
                        gCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGC‑CTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTA‑CGCCGCATCCGACCTCAATGCCTGATGCGAc                                                                                                                                                                                                                                                                >  2:347576/1‑288 (MQ=21)
                                 tGTAGTACGGATAATGCGTTTACGCCGCATCAGACATCAACGCCTGGTGCGACGCTTGCCGCGTCTTAGCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGC‑CTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTA‑CGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCcgcg                                                                                                                                                                                                                                                      <  1:410520/289‑1 (MQ=11)
                                                                                                  ttATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGC‑CTGATTTAGCGTGATTTTCTAGGTCGGATAAGGCGTTTA‑CGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCTGGCCTACGCCCGAAAGGACCATCGCCTGATTGCGTGTGACTTTTTAGGTCGGATacg                                                                                                                                                                                     >  2:492238/1‑287 (MQ=11)
                                                                                                                                                                            gCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGC‑CTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTA‑TGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTg                                                                                                           >  2:158928/1‑289 (MQ=12)
                                                                                                                                                                                       cAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGC‑CTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTA‑TGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTc                                                                                                >  2:16389/1‑289 (MQ=255)
                                                                                                                                                                                               gATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGC‑CTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTA‑TGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAActct                                                                                         >  1:50821/1‑288 (MQ=255)
                                                                                                                                                                                                aTGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGC‑CTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTA‑TGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGATGCTTGCCGCGTCTTTTCAGGCCTATCTTAAACGGTGGCTACTTTTTCAAACTCtt                                                                                        >  2:604517/1‑288 (MQ=11)
                                                                                                                                                                                                 tGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGC‑CTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTA‑TGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGTATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTc                                                                                       >  1:712466/1‑288 (MQ=255)
                                                                                                                                                                                                      cGCTTGCCGCGTCTTGTCAGGCCTACGCCAGACAGCGCAAGAGG‑CTGATTTAGCGTGAGTTTGTAGGTCGGATAAGGCGTTTA‑TGCCGCATCCGACATCAACGCCTTATGCGACGCTTAAAGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCACGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACAcc                                                                                  <  1:615906/288‑1 (MQ=14)
                                                                                                                                                                                                      cGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGC‑CTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTA‑TGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACAccc                                                                                 <  1:16389/289‑1 (MQ=255)
                                                                                                                                                                                                                gTCTTATCAGGCCTACGCCAGACAGCGCAATAGC‑CTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTA‑TGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCaaa                                                                        <  1:27301/288‑1 (MQ=255)
                                                                                                                                                                                                                gTCTTATCAGGCCTACGCCAGACAGCGCAATAGC‑CTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTA‑TGCCGCATCAGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCaaa                                                                        <  1:300444/288‑1 (MQ=255)
                                                                                                                                                                                                                 tCTTATCAGGCCTACGCCAGACAGCGCAATAGC‑CTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTA‑TGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAAc                                                                       >  2:306222/1‑288 (MQ=255)
                                                                                                                                                                                                                   ttATCAGGCCTACGCCAGACAGCGCAATAGC‑CTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTA‑TGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCACACTCTTCACACCCGGTATCAAACCCt                                                                    >  1:286987/1‑289 (MQ=255)
                                                                                                                                                                                                                       cAGGCCTACGCCAGCCAGCGAAATAGC‑CTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTA‑TGCCGCATCCGACATCAACGCCGGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCa                                                                <  1:690661/289‑1 (MQ=255)
                                                                                                                                                                                                                           ccTACGCCAGACAGCGCAATAGC‑CTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTA‑TGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACa                                                            >  1:215208/1‑289 (MQ=255)
                                                                                                                                                                                                                              aCGCCAGACAGCGCAATAGC‑CTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTA‑TGCCGCATCCGACATCCACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCt                                                         <  1:204092/289‑1 (MQ=255)
                                                                                                                                                                                                                              aCGCCAGACAGCGCAATAGC‑CTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTA‑TGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCt                                                         <  1:347576/289‑1 (MQ=255)
                                                                                                                                                                                                                               cGCCAGACAGCGCAATAGC‑CTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTA‑TGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAACCCCTTCCATACAGCt                                                         >  1:640629/1‑288 (MQ=255)
                                                                                                                                                                                                                               cGCCAGACAGCGCAATAGC‑CTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTA‑CGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTc                                                        >  2:170330/1‑289 (MQ=255)
                                                                                                                                                                                                                                    gACAGCGCAATAGC‑CTGCTTTAGCGTGATTTTGTAGGTCGGATAATGCGTTTA‑TGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAATCGTTGGTTAATTTTTCAACCTCTTCACACCCGGTATCATATCCTTCCAttcagttcagct                                                    >  2:289075/1‑286 (MQ=14)
                                                                                                                                                                                                                                     aCAGCGCAATAGC‑CTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTA‑TGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTc                                                  >  1:649724/1‑289 (MQ=255)
                                                                                                                                                                                                                                      cAGCGCAATAGC‑CTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTA‑TGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCa                                                 >  2:123791/1‑289 (MQ=255)
                                                                                                                                                                                                                                         cgcAATAGC‑CTGCTTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTA‑TGCCGCATCCGACATCAACGCCTGATGCGCCGCTTAACGCGTCTTACCAGGCCTACGCCAGACAGCGCAATAGCCTGAGTTAGCGTCATTTTGTAGGTCGGATACGGCGTTTACGCCCCATCCGACATCAATCCCTGATGCGCCGCTGGCCGCCTCTTATGACGCCTATCTCAATCGTTGGTTAATCTTTCAACCTCTGCACACCCTCAATCACACCCTTCCATACAGCTCAGGTTCCAcg                                              >  2:537642/1‑288 (MQ=14)
                                                                                                                                                                                                                                         cgcAATAGC‑CTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTA‑TGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAAc                                               >  2:719158/1‑288 (MQ=255)
                                                                                                                                                                                                                                         cgcAATAGC‑CTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTA‑TGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAAc                                               >  1:428322/1‑288 (MQ=255)
                                                                                                                                                                                                                                          gcAATAGC‑CTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTA‑TGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAAc                                               <  1:123791/287‑1 (MQ=255)
                                                                                                                                                                                                                                                      aTTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTA‑TGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCt                                  <  1:454840/289‑1 (MQ=255)
                                                                                                                                                                                                                                                       tttAGCGTGATTTTGTAGGTCGGATAAGGCGTTTA‑TGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCt                                  <  1:154035/288‑1 (MQ=255)
                                                                                                                                                                                                                                                            cGTGATTTTGTAGGTCGGATAAGGCGTTTA‑TGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCCGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCaaa                                                                        >  2:613077/1‑245 (MQ=255)
                                                                                                                                                                                                                                                            cGTGATTTTGTAGGTCGGATAAGGCGTTTA‑TGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCaaa                                                                        <  1:613077/245‑1 (MQ=255)
                                                                                                                                                                                                                                                                            ggAGAAGGCGTTTA‑CGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATaaa            <  1:492238/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                                     gTTTA‑TGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACcttctt    <  2:215208/288‑1 (MQ=255)
                                                                                                                                                                                                                                                                                     gTTTA‑TGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACcttctt    <  1:325279/288‑1 (MQ=255)
                                                                                                                                                                                                                                                                                       ttA‑TGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCa  >  2:596732/1‑288 (MQ=255)
                                                                                                                                                                                                                                                                                        tA‑TGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGCTTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCaaa                                                                                             >  2:578352/1‑196 (MQ=255)
                                                                                                                                                                                                                                                                                        tA‑TGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCaaa                                                                                             <  1:578352/196‑1 (MQ=255)
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CAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGC‑CTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTA‑TGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTA‑‑CCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCA  >  NC_000913/4295987‑4296550

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑