| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 2,357,836 | G→A | 9.3% | pseudogene (33/198 nt) | ypaA → | pseudogene, C‑terminal fragment, Transposase_31 family |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,357,836 | 0 | G | A | 9.3% | 185.8 / 7.8 | 65 | pseudogene (33/198 nt) | ypaA | pseudogene, C‑terminal fragment, Transposase_31 family |
| Reads supporting (aligned to +/- strand): ref base G (35/23); new base A (3/3); total (38/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.80e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.37e-01 | |||||||||||
ACAAGCCATTAAAATTGCTTTGCGCATGCTGGAGCAGGGCTTTGAACGTGAGATTGTGCTGGCGACAACCCAACTCACTGATGCTGATATTCCGAACTGTCATTAAAAGGAGAGATTAATGACTATTGCTGAACGGCTGCGGCAGGAAGGACATCAAATTGGCTGGCAGGAAGGTAAATTAGAAGGTTTGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTGGAACAGGGCTTTGATCGTGACCAGGTGCTCGCGGCCACCCAGCTAAGCGAAGC > NC_000913/2357698‑2357976 | aCAAGCCATTAAAATTGCTTTGCGCATGCTGGAGCAGGGCTTTGAACGTGAGATTGTGCTGGCGACAACCCAACTCACTGATGCTGATATTCCGAACTGTCATTAAAATGACAGATTAATGACTATTGCTGAACGGCTGCGGCaggaag < 8:374141/149‑1 (MQ=255)aCAAGCCATTAAAATTGCTTTGCGCATGCTGGAGCAGGGCTTTGAACGTGAGATTGTGCTGGCGACAACCCAACTCACTGATGCTGATATTCCGAACTGTCATTAAAAGGAGAGATTAATGACTATTGCTGAACGGCTGCGGCaggaag < 1:337808/149‑1 (MQ=255) gCCATTAAAATTGCTTTGCGCATGCTGGAGCAGGGCTTTGAACGTGAGATTGTGCTGGCGACAACCCAACTCACTGATGCTGATATTCCGAACTGTCATTAAAAGGAGAGATTAATGACTATTGCTGAACGGCTGCGGCAGGAAGGACa < 3:184666/149‑1 (MQ=255) aTTAAAATTGCTTTGCGCATGCTGGAGCAGGGCTTTGAACGTGAGATTGTGCTGGCGACAACCCAACTCACTGATGCTGATATTCCGAACTGTCATTAAAAGGAGAGATTAATGACTATTGCTGAACGGCTGc < 4:349814/133‑1 (MQ=255) aTTAAAATTGCTTTGCGCATGCTGGAGCAGGGCTTTGAACGTGAGATTGTGCTGGCGACAACCCAACTCACTGATGCTGATATTCCGAACTGTCATTAAAAGGAGAGATTAATGACTATTGCTGAACGGCTGc > 3:349814/1‑133 (MQ=255) aTTAAAATTGCTTTGCGCATGCTGGAGCAGGGCTTTGAACGTGAGATTGTGCTGGCGACAACCCAACTCACTGATGCTGATATTCCGAACTGTCATTAAAAGGAGAGATTAATGACTATTGCTGAACGGCTGCGGCAGGAAGGACATc > 4:281586/1‑148 (MQ=255) aTTGCTTTGCGCATGCTGGAGCAGGGCTTTGAACGTGAGATTGTGCTGGCGACAACCCAACTCACTGATGCTGATATTCCGAACTGTCATTAAAAGGAGAGATTAATGACTATTGCTGAACGGCTGCGGCAGGAAGGACATCAAATtgg > 1:377562/1‑149 (MQ=255) gCTTTGCGCATGCTGGAGCAGGGCTTTGAACGTGAGATTGTGCTGGCGACAACCCAACTCACTGATGCTGATATTCCGAACTGTCATTAAAAGGAGAGATTAATGACTATTGCTGAACGGCTGCGGCAGGAAGGACa < 4:162355/137‑1 (MQ=255) gCTTTGCGCATGCTGGAGCAGGGCTTTGAACGTGAGATTGTGCTGGCGACAACCCAACTCACTGATGCTGATATTCCGAACTGTCATTAAAAGGAGAGATTAATGACTATTGCTGAACGGCTGCGGCAGGAAGGACa > 3:162355/1‑137 (MQ=255) gcATGCTGGAGCAGGGCTTTGAACGTGAGATTGTGCTGGCGACAACCCAACTCACTGATGCTGATATTCCGAACTGTCATTAAAAGGAGAGATTAATGACTATTGCTGAACGGCTGCGGCAGGAAGGACATCAAATTGGCTGGCAGGaa < 7:323078/149‑1 (MQ=255) aTGCTGGAGCAGGGCTTTGATCGTGAGATTGTGCTGGCGGCCACCCAACTCACGGATGCGGATATTCCGGACTGTCAGGAAAAGGTGAGATTAAGGACTTTTGCTGAACGGCTGGGGGAGGATGGGGATCAATGTGGCTGGCAGGTAgg > 8:341668/1‑149 (MQ=255) cTGGCGCAGGGCTCTGAACGTGAGATTGTGCTGGCGACAACCCCACTCACTGATGCTGATATTCCGAACTGTCATTAAAAGGAGAGATTAATGACTATTGCTGAACGGCTGCGGCAGTAAGGACATCAAATTGGCTGGCAGGAAGGTaa < 6:331839/149‑1 (MQ=255) tGGAGCAGGGCTTTGAACGTGAGATTGTGCTGGCGACAACCCAACTCAGAGATGCGGATATTCCGAACTGTCATTAAAAGGAGAGCTTAATGACTATTGCTGAACGTCTGCGGCTGGAAGGACATCAAATTGGCTGGAAGGAAGGTaaa > 4:173601/1‑149 (MQ=255) gaacAGGGCTTTGAACGTGAGATTGAGCTGCCGACAACCAAACTCACTCATGCTGATATTACGAACTGTCATTAAAAGGAGAGATAAAAGACTATTGCTGAACGGCTGCGGCAGGAAGGGCATGAAATTGGCTGGCAGGAAGGTAAAt > 8:194940/4‑148 (MQ=25) gCTTTGAACGTGAGATTGTGCTGGCGACAACCCAACTCACTGATGCTGATATTCCGAACTGTCATTAAAAGGAGAGATTAATGACTATTGCTGAACGGCTGCGGCAGGAAGGACATCAAATTGGCTGGCAGGAAGGTAAATTAGAAGGt > 8:239750/1‑149 (MQ=255) gTGAGATTGTGCTGGCGACAACCCAACTCACTGATGCTGATATTCCGAACTGTCATTAAAAGGAGAGATTAATGACTATTGCTGAACGGCTGCGGCAGGAAGGACATCAAATTGGCTGGCAGGAAGGTAAATTAGAAGGTTTGCATGaa > 5:39699/1‑149 (MQ=255) gTGAGATTGTGCTGGCGACAACCCAACTCACTGATGCTGATATTCCGAACTGTCATTAAAAGGAGAGATTAATGACTATTGCTGAACGGCTGCGGCAGGAAGGACATCAAATTGGCTGGCAGGAAGGTAAATTAGAAGGTTTGCATGaa > 1:36262/1‑149 (MQ=255) actctgCCGGCCACAACCCAACTCACTGAAGCTGATATTCCGAACTGTCATTAAAACGAGAGATTAATGACTATTACTGAACGGCTGCGTCAGGAAGGACATCAAATTGGCTGGCAGGAAGGTAAATTAGAAGGTTTGCATGAACAAGc < 8:119356/145‑1 (MQ=37) aTTGTGCTGGCGACAACCCAACTCACTGATGCTGATATTCCGAACTGTCATTAAAAGGAGAGATTAATGACTATTGCTGAACGGCTGCGGCAGGAAGGACATCAAATTGGCTGGCAGGAAGGTAAATTAGAAGGTTTGCATGAACAAGc < 3:281586/149‑1 (MQ=255) gCTGGCGACAACCCAACTCACTGATGCTGATATTCCGAACTGTCATTAAAAGGAGAGACTAATGACTATTGCTGAACGGCTGCGGCAGGAAGGACATCAAATTGGCTGGCAGGAAGGTAAATTAGAAGGTTTGCATGAACAAGCCAtt > 7:149185/1‑148 (MQ=255) gCGACAACCCAACTCACTGATGCTGATATTCCGAACTGTCATTAAAAGGAGAGATTAATGACTATTGCTGAACGGCTGCGGCAGGAAGGACATCAAATTGGCTGGCAGGAAGGTAAATTAGAAGGTTTGCATGAACAAGCCATTaaaa < 6:39699/148‑1 (MQ=255) cGACAACCCAACTCACTGATGCTGATATTCCGAACTGTCATTAAAAGGAGAGATTAATGACTATTGCTGAACGGCTGCGGCAGGAAGGACATCAAATTGGCTGGCAGGAAGGTAAATTAGAAGGTTTGCATGAACAAGCCATTAAAAtt < 7:239750/149‑1 (MQ=255) aCCCAACTCACTGATGCTGATATTCCGAACTGTCATTAAAAGGAGAGATTAATGACTATTGCTGAAAGGCTGCGGCAGGAAGGACATCAAATTGGCTGGCAGGAAGGTAAATTAGAAGGTTTGCATGAACAAGCCATTAAAATTGCttt < 2:377562/149‑1 (MQ=255) cccAACTCACTGATGCTGATATTCCGAACTGTCATTAAAAGGAGAGATTAATGACTATTTCTGAACGGCTGCGGCAGGAAGGACATCAAATTGGCTGGCAGGAAGGTAAATTAGAAGGTTTGCATGAACAAGCCATTAAAATTGCTTTg > 8:379991/1‑149 (MQ=255) cccAACTCACTGATGCTGATATTCCGAACTGTCATTAAAAGGAGAGATTAATGACTATTGCTGAACGGCTGCGGCAGGAAGGACATCAAATTGGCTGGCAGGAAGGTAAATTAGAAGGTTTGCATGAACAAGCCATTAAAATTGCTTTg > 2:338772/1‑149 (MQ=255) cccAACTCACTGATGCTGATATTCCGAACTGTCATTAAAAGGAGAGATTAATGACTATTGCTGAACGGCTGCGGCAGGAAGGACATCAAATTGGCTGGCAGGAAGGTAAATTAGAAGGTTTGCATGAACAAGCCATTAAAATTGCTTTg > 1:39166/1‑149 (MQ=255) aCTGATGCTGATATTCCGAACTGTCATTAAAAGGAGAGATTAATGACTATTGCTGAACGGCTGCGGCAGGAAGGACATCAAATTGGCTGGCAGGAAGGTAAATTAGAAGGTTTGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCt > 3:363665/1‑149 (MQ=255) aTGCTGATATTCCGAACTGTCATTAAAAGGAGAGATTAATGACTATTGCTGAACGGCTGCGGCAGGAAGGACATCAAATTGGCTGGCAGGAAGGTAAATTAGAAGGTTTGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTGGaa > 6:281644/1‑149 (MQ=255) tGATATTCCGAACTGTCATTAAAAGGAGAGATTAATGACTATTGCTGAACGGCTGCGGCAGGAAGGACATCAAATTGGCTGGCAGGAAGGTAAATTAGAAGGTTTGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTGGAACAgg > 3:136531/1‑149 (MQ=255) atatTCCGAACTGTCATTAAAAGGAGAGATTAATGACTATTGCTGAACGGCTGCGGCAGGAAGGACATCAAATTGGCTGGCAGGAAGGTAAATTAGAAGGTTTGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTGGAACAGGGc > 4:299641/1‑149 (MQ=255) cGAACTGTCATTAAAAGGAGAGATTAATGACTATTGCTGAACGGCTGCGGCAGGAAGGACATCAAATTGGCTGGCAGGAAGGTAAATTAGAAGGTTTGCATGAACAAGCCATTaa > 6:175544/1‑115 (MQ=255) cGAACTGTCATTAAAAGGAGAGATTAATGACTATTGCTGAACGGCGGCGGCAGGAAGGACATCAAATTGTCTGGCAGGAAGGTAAATTAGAAGGTTTGCATGAAAAAGCCATTAAAATTGCTTTGCGCATGCTGGAACAGGGCTTTGa < 7:194940/148‑1 (MQ=255) aCTGTCATTAAAAGGAGAGATTAATGACTATTGCTGAACGGCTGCGGCAGGAAGGACATCAAATTGGCTGGCAGGAAGGTAAATTAGAAGGTTTGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTGGAACAGGGCTTTGATCGt < 7:379991/149‑1 (MQ=255) aCTGTCATTAAAAGGAGAGATTAATGACTATTGCTGAACGGCTGCGGCAGGAAGGACATCAAATTGGCTGGCAGGAAGGTAAATTAGAAGGTTTGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTGGAACAGGGCTTTGATCGt < 2:39166/149‑1 (MQ=255) tGTCATTAAAAGGAGAGATTAATGACTATTGCTGAACGGCTGCGGCAGGAAGGACATCAAATTGGCTGGCAGGAAGGTAAATTAGAAGGTTTGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTGGAACAGGGCTTTGATc > 1:288529/1‑145 (MQ=255) tGTCATTAAAAGGAGAGATTAATGACTATTGCTGAACGGCTGCGGCAGGAAGGACATCAAATTGGCTGGCAGGAAGGTAAATTAGAAGGTTTGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTGGAACAGGGCTTTGATc < 2:288529/145‑1 (MQ=255) gTCATTAAAAGGAGAGATTAATGACTATTGCTGAACGGCTGCGGCAGGAAGGACATCAAATTGGCTGGCAGGAAGGTAAATTAGAAGGTTTGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTg < 6:98209/128‑1 (MQ=255) gTCATTAAAAGGAGAGATTAATGACTATTGCTGAACGGCTGCGGCAGGAAGGACATCAAATTGGCTGGCAGGAAGGTAAATTAGAAGGTTTGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTg > 5:98209/1‑128 (MQ=255) tCATTAAAAGGAGAGATTAATGACTATTGCTGAACGGCTGCGGCAGGAAGGACATCAAATTGGCTGGCAGGAAGGTAAATTAGAAGGTTTGCATGAACAAGCCATTAAAATTGCttt > 5:39689/1‑117 (MQ=255) tCATTAAAAGGAGAGATTAATGACTATTGCTGAACGGCTGCGGCAGGAAGGACATCAAATTGGCTGGCAGGAAGGTAAATTAGAAGGTTTGCATGAACAAGCCATTAAAATTGCttt < 6:39689/117‑1 (MQ=255) aTTAAAAGGAGAGATTAATGACTATTGCTGAACGGCTGCGGCAGGAAGGACATCAAATTGGCTGGCAGGAAGGTAAATTAGAAGGTTTGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTGGAACAGGGCTTTGATCGTGACCAg > 8:287008/1‑149 (MQ=255) ttAAAAGGAGAGATTAATGACTATTGCTGAACGGCTGCGGCAGGAAGGACATCAAATTGGCTGGCAGGAAGGTAAATTAGAAGGTTTGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTGGAACAGGGCTTTGATCGTGACCAgg > 7:362943/1‑149 (MQ=255) ttAAAAGGAGAGATTAATGACTATTGCTGAACGGCTGCGGCAGGAAGGACATCAAATTGGCTGGCAGGAACGTAAATTAGAAGGTTTGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTGGAACAGGGCTTTGATCGTGACCAgg > 5:320376/1‑149 (MQ=255) caaaaGGAGAGATTAATGACTATTGCTGAACGGCTGCGGCaggaa < 1:204124/44‑1 (MQ=25) caaaaGGAGAGATTAATGACTATTGCTGAACGGCTGCGGCaggaa > 2:204124/2‑45 (MQ=25) ggAGAGATTAATGACTATTGCTGAACGGCTGCGGCAGGAAGGACATCAAATTGGCTGGCAGGAAGGTAAATTAGAAg < 2:101271/77‑1 (MQ=255) ggAGAGATTAATGACTATTGCTGAACGGCTGCGGCAGGAAGGACATCAAATTGGCTGGCAGGAAGGTAAATTAGAAg > 1:101271/1‑77 (MQ=255) gagagaTTAATGACTATTGCTGAACGGCTGCGGCAGGAAGGACATCAAATTGGCTGGCAGGAAGGTAAATTAGAAGGTTTGCATGAACAAGCCATTAAAATTGCTTTGCGCATGATGGAACAGGGCTTTGATCGTGACCAGGTGCTcgc > 7:122371/1‑149 (MQ=255) gagaTTAATGACTATTGCTGAACGGCTGCGGCAGGAAGGACATCAAATTGGCTGGCAGGAAGGTAAATTAGAAGGTTTGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTGGAACAGGGCTTTGATCGTGACCAGGTGCTCGCgg > 8:232555/1‑149 (MQ=255) agaTTAATGACTATTGCTGAACGGCTGCGGCAGGAAGGACATCAAATTGGCTGGCAGGAAGGTAAATTAGAAGGTTTGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTGGAACAGGGCTTTGATCGTGACCAGGTGCTCGCGGc < 4:136531/149‑1 (MQ=255) actAATGACTATTGCTGAACGGCTGCGGCAGGAAGGACATCAAATTGGCTGGCAGGAAGGTAAATTAGAAGGTTTGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTGGAACAGGGCTTTGATCGTGACCAGGTGCTCGCGGCCa < 8:149185/147‑1 (MQ=255) aTGACTATTGCTGAACGGCTTCGGCAGGAAGGACATCAAATtggctggc < 8:405206/49‑1 (MQ=255) aTGACTATTGCTGAACGGCTGCGGCAGGAAGGACATCAAATTGGCTGGCAGGAAGGTAAATTAGAAGGTTTGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTGGAACAGGGCTTTGATCGTGACCAGGTGCTCGCGGCCAcc > 7:139270/1‑147 (MQ=255) tGACTATTGCTGAACGGCTACGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTAAATTAGAAGGTTTGCATGAACAAGCCa > 8:141063/1‑84 (MQ=255) tGACTATTGCTGAACGGCTACGGCAGGAAGGACATCAAATTGGCTGGCAGGAAGGTAAATTAGAAGGTTTGCATGAACAAGCCa < 7:141063/84‑1 (MQ=255) aCTATTGCTGAACGGCTACGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTAAATTAGAAg > 2:10884/1‑64 (MQ=255) aCTATTGCTGAACGGCTACGGCAGGAAGGACATCAAATTGGCTGGCAGGAAGGTAAATTAGAAg < 1:10884/64‑1 (MQ=255) tATTGCTGATCGGCTACGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTAAATTAGAAGGTTTGc > 8:54845/1‑68 (MQ=14) tATTGCTGATCGGCTACGGCAGGAAGGACATCAAATTGGCTGGCAGGAAGGTAAATTAGAAGGTTTGc < 7:54845/68‑1 (MQ=255) ttGCTGTACGGCTGCGGCAGGAAGGACATCAAATTGGCTGGCAGGAAGTTAAATTATAATGTTTGCATGATCAAGCCATTAAAATTGCTTTGCGCATGCTGGTACAGGGCTTTGATCGTGACCAGGTGCTCGAGGCCACCCAGCTAAGc < 6:320376/149‑1 (MQ=38) gCTGAACGGCTGCGGCAGGAAGGACATCAAATTGGCTGGCAGGAAGGTAAATTAGAAGGTTTGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTGGAACAGGGCTTTGATCGTGACCAGGTGCTCGCGGCCACCCAGCTAAGCg > 3:48515/1‑148 (MQ=255) cTGAACGGCTGCGGCAGGAAGGACATCAAATTGGCTGGCAGGAAGGTAAATTAGAAGGTTTGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTGGAACAGGGCTTTGATCGTGACCAGGTGCTCGCGGCCACCCAGCTAAGCg > 5:347016/1‑147 (MQ=255) cTGAACGGCTGCGGCAGGAAGGACATCAAATTGGCTGGCAGGAAGGTAAATTAGAAGGTTTGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTGGAACAGGGCTTTGATCGTGACCAGGTGCTCGCGGCCACCCAGCTAAGCg > 2:399286/1‑147 (MQ=255) tGAACGGCTGCGGCAGGAAGGACATCAAATTGGCTGGCAGGAAGGTAAATTAGAAGGTTTGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTGGAACAGGGCTTTGATCGTGACCAGGTGCTCGCGGCCACCCAGCTAAGCGAAg > 5:58405/1‑149 (MQ=255) gAACGGCGGCGGCAGGAAGGACATCAAATTGGCTGGCAGGAAGGTAAAATAGAAGGTTTGCATGAACAAGCCATTAAAATTGCATTGCGCATGCTGGAACAGGGCTTTGATCGTGACCAGGTGCTCGCGGCCACCCAGCTAAGCGAAGc > 6:343923/1‑149 (MQ=255) | ACAAGCCATTAAAATTGCTTTGCGCATGCTGGAGCAGGGCTTTGAACGTGAGATTGTGCTGGCGACAACCCAACTCACTGATGCTGATATTCCGAACTGTCATTAAAAGGAGAGATTAATGACTATTGCTGAACGGCTGCGGCAGGAAGGACATCAAATTGGCTGGCAGGAAGGTAAATTAGAAGGTTTGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTGGAACAGGGCTTTGATCGTGACCAGGTGCTCGCGGCCACCCAGCTAAGCGAAGC > NC_000913/2357698‑2357976 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |