Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,753,945 T→C 6.0% K178R (AAA→AGA)  ydhY ← putative 4Fe‑4S ferridoxin‑type protein; FNR, Nar, NarP‑regulated protein; putative subunit of YdhYVWXUT oxidoreductase complex

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,753,9450TC 6.0% 202.4 / 3.1 67K178R (AAA→AGA) ydhYputative 4Fe‑4S ferridoxin‑type protein; FNR, Nar, NarP‑regulated protein; putative subunit of YdhYVWXUT oxidoreductase complex
Reads supporting (aligned to +/- strand):  ref base T (31/32);  new base C (2/2);  total (33/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.04e-01

TCTTAATATATTACCTGTCCAACCGTTAGCCATGATTTTTTCCTTTGCAAGATTCACACAGTAATATCTTTCCACTCGATAATTTTTAACGCCCCTGTTGGGCAGGCGTTTGCGCATTCACCGCATAACACACATTTCGAGGATTTTTTACTTTCGGTATTTACGGTGGCCATCATCCACGGGCATGCCGTAGTACAGGCGCTACAGCCAATACAGCGTTTATGATCGACGGTAATACAGCCTTCTTTCTGCTGCCAGGTAATCGCACCAATCGGGCAGACGTTCATGCATTGCG  >  NC_000913/1753798‑1754092
                                                                                                                                                   |                                                                                                                                                   
tctTAATATATTACCTGTCCAACCGTTAGCCATGATTTTTTCCTTTGCAAGATTCACACAGTAATATCTTTCCACTCGATAATTTTTAACGCCCCTGTTGGGCAGGCGTTTGCGCATTCACCGCATAACACACATTTCGAGGAttttt                                                                                                                                                     >  2:150781/1‑148 (MQ=255)
tctTAATATATTACCTGTCCAACCGTTAGCCATGATTTTTTCCTTTGAAAGATTCACACAGTAATATCTTTCCACTCGATAATTTTTAACGCCCCTGTTGGGCAGGCGTTTGCGCATTCACCGCATAACACACATTTCGAGGAttttt                                                                                                                                                     >  3:153605/1‑148 (MQ=255)
  ttAATATATTACCTGTCCAACCGTTAGCCATGATTTTTTCCTTTGCAAGATTCACACAGTAATATCTTTCCACTCGATAATTTTTAACGCCCCTGTTGGGCAGTCGTTTGCGCATTCACCGCATAACACACATTTAGAGGATTTTTTAc                                                                                                                                                  >  5:250685/1‑149 (MQ=255)
  ttAATATATTACCTGTCCAACCGTTAGCCATGATTTTTTCCTTTGCAAGATTCAAACAGTAATATCTTTCCACTCGATAATTTTTAACGCCCCTGTTGGGCAGGCGTTTGCGCATTCACCGCATAACACACATTTCGAGGAtttttt                                                                                                                                                    <  6:45381/147‑1 (MQ=255)
    aaTATATTACCTGTCCAACCGTTAGCCATGATTTTTTCCTTTGCAAGATTCACACAGTAATATCTTTCCACTCGATAATTTTTAACGCCCCTGTTGGGCAGGCGTTTGCGCATTCACCGCATAACACACATTTCGAGGATTTTTTAc                                                                                                                                                  <  4:206024/147‑1 (MQ=255)
        tatTACCTGTCCAACCGTTAGCCATGATTTTTTCCTTTGCAAGATTCACACAGTAATATCTTTCCACTCGATAATTTTTAACGCCCCTGTTGGGCAGGCGTTTGCGCATTCACCGCATAACACACATTTCGAGGATTTTTTACTTTCgg                                                                                                                                            >  6:298080/1‑149 (MQ=255)
        tatTACCTGTCCAACCGTTAGCCATGATTTTTTCCTTTGCAAGATTCACACAGTAATATCTTTCCACTCGATAATTTTTAACGCCCCTGTTGGGCAGGCGTTTGCGCATTCACCGCATAACACACATTTCGAGGATTTTTTACTTTCg                                                                                                                                             <  5:109083/148‑1 (MQ=255)
          ttACCTGTCCAACCGTTAGCCATGATTTTTTCCTTTGCAAGATTCACACAGTAATATGTTTCCACTCGATAATTTTTAACGCCCCTGTTGGGCAGGCGTTTGCGCATTCACCGCATAACACACATTTCGAGGATTTTTTACTTTCGGTa                                                                                                                                          >  8:248443/1‑149 (MQ=255)
           tACCTGTCCAACCGTTAGCCATGATTTTTTCCTTTGCAAGATTCACACAGTAATATCTTTCCACTCGATAATTTTTAACGCCCCTGTTGGGCAGGCGTTTGCGCATTAACCGCATAACACACATTTCGAGGATTTTTTACTTTCGGTAt                                                                                                                                         >  5:374430/1‑149 (MQ=255)
             ccTGTCCAACCGTTAGCCATGATTTTTTCCTTTGCAAGATTCACACAGTAATATCTTTCCACTCGATAATTTTTAACGCCCCTGTTGGGCAGGCGTTTGCGCATTCACCGCATAACACACATTTCGAGGATTTTTTACTTTCGGTAtt                                                                                                                                        >  5:252933/1‑148 (MQ=255)
                    aaCCGTTAGCCATGATTTTTTCCTTTGCAAGATTCACACAGTAATATCTTTCCACTCGATAATTTTTAACGCCCCTGTTGGGCAGGCGTTTGCGCATTCACCGCATAACACACATTTCGAGGATTTTTTACTTTCGGTATTTACGGTgg                                                                                                                                <  8:173478/149‑1 (MQ=255)
                    aaCCGTTAGCCATGATTTTTTCCTTTGCAAGATTCACACAGTAATATCTTTCCACTCGATAATTTTTAACGCCCCTGTTGGGCAGGCGTTTGCGCATTCACCGCATAACACACATTTCGAGGATTTTTTACTTTCGGTATTTACGGTgg                                                                                                                                <  3:165815/149‑1 (MQ=255)
                       cGTTAGCCATGATTTTTTCCTTTGCAAGATTCACACAGTAATATCTTTCCACTCGATAATTTTTAACGCCCCTGTTGGGCAGGCGTTTGCGCATTCACCGCATAACACACATTTCGAGGATTTTTTACTTTCGGTATTTACGGTGGCca                                                                                                                             >  8:140554/1‑149 (MQ=255)
                       cGTTAGCCATGATTTTTTCCTTTGCAAGATTCACACAGTAATATCTTTCCACTCGATAATTTTTAACGCCCCTGTTGGGCAGGCGTTTGCGCATTCACCGCATAACACACATTTCGAGGATTTTTTACTTTCGGTATTTACGGTGGCca                                                                                                                             <  6:193365/149‑1 (MQ=255)
                       cGTTAGCCATGATTTTTTCCTTTGCAAGATTCACACAGTAATATCTTTCCACTCGATAATTTTTAACGCCCCTGTTGGGCAGGCGTTTGCGCATTCACCGCATAACACACATTTCGAGGATTTTTTACTTTCGGTATTTACGGTGGCca                                                                                                                             <  3:359439/149‑1 (MQ=255)
                            gCCATGATTTTTTCCTTTGCAAGATTCACACAGTAATATCTTTCCACTCGATAATTTTTAACGCCCCTGTTGGGCAGGCGTTTGCGCATTCACCGCATAACACACATTTCGAGGATTTTTTACTTTCGGTATTTACGGTGGCCatcatc                                                                                                                        >  3:298776/1‑149 (MQ=255)
                             ccATGATTTTTTCCTTTGCAAGATTCACACAGTAATATCTTTCCACTCGATAATTTTTAACGCCCCTGTTGGGCAGGCGTTTGCGCATTCACCGCATAACACACATTTCGAGGATTTTTTACTTTCGGTATTTACGGTGGCCatcatc                                                                                                                        >  8:148706/1‑148 (MQ=255)
                                tGATTTTTTCCTTTGCAAGATTCACACAGTAATATCTTTCCACTCGATAATTTTTAACGCCCCTGTTGGGCAGGCGTTTGCGCATTCACCGCATAACACACTTTTCGAGGATTTTTTACTTTCGGTATTTACTGTGGCCATCATCCACg                                                                                                                    >  2:61804/1‑149 (MQ=255)
                                      tttCCTTTGCAAGATTCACACAGTAATATCTTTCCACTCGATAATTTTTAACGCCCCTGTTGGGCAGGCGTTTGCGCATTCACCGCATAACACACATTTCGAGGATTTTTTACTTTCGGTATTTACGGTGGCCATCATCCACGGGCATg                                                                                                              >  3:91304/1‑149 (MQ=255)
                                       ttCCTTTGCAAGATTCACACAGTAATATCTTTCCACTCGATAATTTTTAACGCCCCTGTTGGGCAGGCGTTTGCGCATTCACCGCATAACACACATTTCGAGGATTTTTTACTTTCAGTATTTACGGTGGCCATCATCCACGGGCATGc                                                                                                             >  3:238720/1‑149 (MQ=255)
                                        tCCTTTGCAAGATTCACACAGTAATATCTTTCCACTCGATAATTTTTAACGCCCCTGTTGGGCAGGCGTTTGCGCATTCACCGCATAACACACATTTCGAGGATTTTTTACTTTCGGTATTTACGGTGGCCATCATCCACgg                                                                                                                   >  1:111094/1‑142 (MQ=255)
                                        tCCTTTGCAAGATTCACACAGTAATATCTTTCCACTCGATAATTTTTAACGCCCCTGTTGGGCAGGCGTTTGCGCATTCACCGCATAACACACATTTCGAGGATTTTTTACTTTCGGTATTTACGGTGGCCATCATCCACgg                                                                                                                   <  2:111094/142‑1 (MQ=255)
                                             tGCAAGATTCACACAGTAATATCTTTCCACTCGATAATTTTTAACGCCCCTGTTGGGCAGGCGTTTGCGCATTCACCGCATAACACACATTTCGAGGATTTTTTACTTTCGGTATTTACGGTGGCCATCATCCACGGGCATGCCgta                                                                                                         <  4:91304/147‑1 (MQ=255)
                                                     tCACACAGTAATATCTTTCCACTCGATAATTTTTAACGCCCCTGTTGGGCAGGCGTTTGCGCATTCACCGCATAACACACATTTCGAGGATTTTTTACTTTCGGTATTTACGGTGGCCATCATCCACGGGCATGCCGTAGTACAGgcgc                                                                                               >  6:231002/1‑149 (MQ=255)
                                                           aGTAATATCTTTCCACTCGATAATTTTTAACGCCCCTGTTGGGCAGGCGTTTGCGCATTCACCGCATAACACACATTTCGAGGATTTTTTACTTTCGGTATTTACGGTGGCCATCATCCACGGGCATGCCGTAGTACAGGCGCTACAGc                                                                                         <  3:162274/149‑1 (MQ=255)
                                                             tAATATCTTTCCACTCGATAATTTTTAACGCCCCTGTTGGGCAGGCGTTTGCGCATTCACCGCATAACACACATTTCGAGGATTTTTTACTTTCGGTATTTACGGTGGCCATCATCCACGGGCATGCCGTAGTACAGGCGCTACAGcc                                                                                        >  8:259965/1‑148 (MQ=255)
                                                               atatCTTTCCACTCGATAATTTTTAACGCACCTGTTGGGCAGGCGTTTGCGCATTCACCGCATAACACACATTTCGAGGATTTTTTACTTTCGGTATTTACGGTGGCCATCATCCACGGGCATGCCGTAGTACAGGCGCTACAGCCaa                                                                                      <  2:167471/148‑1 (MQ=255)
                                                                 atCTTTCCACTCGATAATTTTTAACGCCCCTGTTGGGCAGGCGTTTGCGAATTCACCGCATAACACACATTTCGAGGATTTTTTACTTTCGGTATTTACGGTGGCCATCATCCACGGGCATGCCGTAGTACAGGCGCTACAGCCAATa                                                                                    >  1:73256/1‑148 (MQ=255)
                                                                   cTTTCCACTCGATAATTTTTAACGCCCCTGTTGGGCAGGCGTTTGCGCATTCACCGCATAACACACATTACGAGGATTTTTTACTTTCGGTATTTACGGTGGCCATCATCCACGGGCATGCCGTAGTACAGGCGCTACAGCCAATACAg                                                                                 >  6:165050/1‑149 (MQ=255)
                                                                      tCCACTCGATAATTTTTAACGCCCCTGTTGGGCAGGCGTTTGCGCATTCACCGCATAACACACATTTCGAGGATTTTTTACTTTCGGTATTTACGGTGGCCATCATCCACGGGCATGCCGTAGTACAGGCGCTACAGCCAATACa                                                                                  <  7:248443/145‑1 (MQ=255)
                                                                         aCTCGATAATTTTTAACGCCCCTGTTGGGCAGGCGTTTGCGCATTCACCGCATAACACACATTTCGAGGATTTTTTACTTTCGGTATTTACGGTGGCCATCATCCACGGGCATGCCGTAGTACAGGCGCTACAGCCAATACa                                                                                  <  7:319682/142‑1 (MQ=255)
                                                                         aCTCGATAATTTTTAACGCCCCTGTTGGGCAGGCGTTTGCGCATTCACCGCATAACACACATTTCGAGGATTTTTTACTTTCGGTATTTACGGTGGCCATCATCCACGGGCATGCCGTAGTACAGGCGCTACAGCCAATACa                                                                                  >  8:319682/1‑142 (MQ=255)
                                                                           tCGATAATTTTTAACGCCCCTGTTGGGCAGGCGTTTGCGCATTCACCGCATAACACACATTTCGCGGATTTTTTACTTTCGGTATTTACGGTGGCCATCATCCACGGGCATGCCGTAGTACAGGCGCTACAGCCAATACAGCGTTTATg                                                                         >  5:54001/1‑149 (MQ=255)
                                                                             gATAATTTTTAACGCCCCTGTTGGGCAGGCGTTTGCGCATTCACCGCATAACACACATTTCGAGGATTTTTTACTTTCAGTATTTACGGTGGCCATCATCCACGGGCATGCCGTAGTACAGGCGCTACAGCCAATACAGCGTTTATGAt                                                                       <  4:238720/149‑1 (MQ=255)
                                                                                 aTTTTTAACGCCCCTGTTGGGCAGGCGTTTGCGCATTCACCGCATAACACACATTTCGAGGATTTTTTACTTTCGGTATTTACGGTGGCCATCATCCACGGGCATGCCGTAGTACAGGCGCTACAGCCAATACAGCGTTTATGATCGAc                                                                   <  5:298080/149‑1 (MQ=255)
                                                                                 aTTTTTAACGCCCCTGTTGGGCAGGCGTTTGCGCATTCACCGCATAACACACATTTCGAGGATTTTTTACTTTCGGTATTTACGGTGGCCATCATCCACGGGCATGCCGTAGTACAGGCGCTACAGCCAATACAGCGTTTATGATCGAc                                                                   <  6:252933/149‑1 (MQ=255)
                                                                                  tttttAACGCCCCTGTTGGGCAGGCGTTTGCGCATTCACCGCATAACACACATTTCGAGGATTTTTTACTTTCGGTATTTACGGTGGCCATCATCCACGGGCATGCCGTAGTACAGGCGCTACAGCCAATACAGCGTTTATGATCGACg                                                                  <  4:153605/149‑1 (MQ=255)
                                                                                   ttttAACGCCCCTGTTGGGCAGGCGTTTGCGCATTCACCGCATAACACACATTTCGAGGATTTTCTACTTTCGGTATTTACGGTGGCCATCATCCACGGGCATGCCGTAGTACAGGCGCTACAGCCAATACAGCGTTTATGATCGACgg                                                                 >  2:44839/1‑149 (MQ=255)
                                                                                          gCCCCTGTTGGGCAGGCGTTTGCGCATTCACCGCATAACACACATTTCGAGGATTTTTTACTTTCGGTATTTCCGGTGGCCATCATCCACGGGCATGCCGTAGTACAGGCGCTACAGCCAATACAGCGTTTATGATCGACGGTAATACa                                                          <  1:150781/149‑1 (MQ=255)
                                                                                          gCCCCTGTTGGGCAGGCGTTTGCGCATTCACCGCATAACACACATTTCGAGGATTTTTTACTTTCGGTATTTACGGTGGCCATCATCCACGGGCATGCCGTAGTACAGGCGCTACAGCCAATACAGCGTTTATGATCGACGGTAATACa                                                          <  7:259965/149‑1 (MQ=255)
                                                                                          gCCCCTGTTGGGCAGGCGTTTGCGCATTCACCGCATAACACACATTTCGAGGATTTTTTACTTTCGGTATTTACGGTGGCCATCATCCACGGGCATGCCGTAGTACAGGCGCTACAGCCAATACAGCGTTTATGATCGACGGTAATACa                                                          <  3:342031/149‑1 (MQ=255)
                                                                                            cccTGTTGGGCAGGCGTTTGCGCATTCACCGCATAACACACATTTCGAGGATTTTTTACTTTCGGTATTTACGGTGGCCATCATCCACGGGCATGCCGTAGTACAGGCGCTACAGCCAATACAGCGTTTATGATCGACGGTAATACAGc                                                        <  8:78093/149‑1 (MQ=255)
                                                                                            cccTGTTGGGCAGGCGTTTGCGCATTCACCGCATAACACACATTTCGAGGATTTTTTACTTTCGGTATTTACGGTGGCCATCATCCACGGGCATGCCGTAGTACAGGCGCTACAGCCAATACAGCGTTTATGATCGACGGTAATACAGc                                                        <  4:298776/149‑1 (MQ=255)
                                                                                            cccTGTTGGGCAGGCGTTTGCGCATTAACCGCATAAAACACATTTCGAGGATTTTCTACTTTCGGTATTTACGGTGGCCATCATCCACGGGCATGCCGTAGTACAGGCGCTACAGCCAATACAGCGTTTATGATCGACGGTAATACAGc                                                        >  4:308495/1‑149 (MQ=255)
                                                                                             ccTGTTGGGCAGGCGTTTGCGCATTCCCCGCATAACACACATTTCGAGGATTTTTTACTTTCGGTATTTACGGTGGCCATCATCCACGGGCATGCCGTAGTACAGGCGCTACAGCCAATACAGCGTTTATGATCGACGGTAATACAGcc                                                       >  6:333853/1‑149 (MQ=255)
                                                                                             ccTGTTGGGCAGGCGTTTGCGCATTCACCGCATAACACACATTTCGAGGATTTTTTACTTTCGGTATTTACGGTGGCCATCATCCACGGGCATGCCGTAGTACAGGCGCTACAGCCAATACAGCGTTTATGATCGACGGTAATACAGcc                                                       <  5:170552/149‑1 (MQ=255)
                                                                                              cTGTTGGGCAGGCGTTTGCGCATTCACCGCATAACACACATTTCGAGGATTTTCTACTTTCGGTATTTACGGTGGCCATCATCCACGGGCATGCCGTAGTACAGGCGCTACAGCCAATACAGCGTTTATGATCGACGGTAATACAGCct                                                      <  1:44839/149‑1 (MQ=255)
                                                                                                               gcgcATTCACCGCATAACACACATTTCGAGGATTTTTTACTTTCGGTATTTACGGTGGCCATCATCCACGGGCATGCCGTAGTACAGGCGCTACAGCCAATACAGCGTTTATGATCGACGGTAATACAGCCTTCTTTCTGCTGCCAgg                                      >  1:219978/1‑148 (MQ=255)
                                                                                                                 gcATTCACCGCATAACACACATTTCGAGGATTTTTTACTTTCGGTATTTACGGTGGCCATCATCCACGGGCATGCCGTAGTACAGGCGCTACAGCCAATACAGCGTTTATGATCGACgg                                                                 <  8:275043/119‑1 (MQ=255)
                                                                                                                 gcATTCACCGCATAACACACATTTCGAGGATTTTTTACTTTCGGTATTTACGGTGGCCATCATCCACGGGCATGCCGTAGTACAGGCGCTACAGCCAATACAGCGTTTATGATCGACgg                                                                 >  7:275043/1‑119 (MQ=255)
                                                                                                                 gcATTCACCGCATAACACACATTTCGAGGATTTTTTACTTTCGGTATTTACGGTGGACATCATCCACGGGCATGCCGTAGTACAGGCGCTACAGCCAATACAGCGTTTATGATCGACGGTAATACAGCCTTCTTTCTGCTGCCAGGTaa                                   >  4:95022/1‑149 (MQ=255)
                                                                                                                   aTTCACCGCATAACACACATTTCGAGGATTTTTTACTTTCGGTATTTACGGTGGCCATCATCCACGGGCATGCCGTAGTACAGGCGCTACAGCCAATACAGCg                                                                               <  3:50829/103‑1 (MQ=255)
                                                                                                                   aTTCACCGCATAACACACATTTCGAGGATTTTTTACTTTCGGTATTTACGGTGGCCATCATCCACGGGCATGCCGTAGTACAGGCGCTACAGCCAATACAGCg                                                                               >  4:50829/1‑103 (MQ=255)
                                                                                                                   aTTCACCGCATAACACACATTTCGAGGATTTTTTACTTTCGGTATTTACGGTGGCCATCATCCACGGGCATGCCGTAGTACAGGCGCTACAGCCAATACAGCGTTTATGATCGACGGTAATACAGCCTTCTTTCTGCTGCCAGGTAATc                                 <  8:174891/149‑1 (MQ=255)
                                                                                                                       aCCGCATAACACACATTTCGAGGATTTTTTACTTTCGGTATTTACGGTGGCCATCATCCACGGGCATGCCGTAGTACAGGCGCTACAGCCAATACAGCGTTTATGATCGACGGTAATACAGCCTTCTTTCTGCTGCCAGGTAATCGCAc                             >  8:199157/1‑149 (MQ=255)
                                                                                                                        ccGCATAACACACATTTCGAGGATTTTTTACTTTCGGTATTTACGGTGGCCATCATCCACGGGCATGCCGTAGTACAGGCGCTACAGCCAATACAGCGTTTATGATCGACGGTAATACAGCCTTCTTTCTGCTGCCAGGTAATCGCAc                             >  6:41886/1‑148 (MQ=255)
                                                                                                                          gCATAACACACATTTCGAGGATTTTTTACTTTCGGTATTTACGGTGGCCATCATCCACGGGCATGCCGTAGTACAGGCGCTACAGCAAATACAGCGTTTATGATCGACGGTAATACAGCATTCTTTCTGCTGCCAGGTAATCGCACCaa                          >  7:208876/1‑149 (MQ=255)
                                                                                                                           cATAACACACATTTCGAGGATTTTTTACTTTCGGTATTTACGGTGGCCATCATCCACGGGCATGCCGTAGTACAGGCGCTACAGCCAATACAGCGTTTATGATCGACGGTAATACAGCCTTCTTTctgct                                            >  2:297658/1‑130 (MQ=255)
                                                                                                                           cATAACACACATTTCGAGGATTTTTTACTTTCGGTATTTACGGTGGCCATCATCCACGGGCATGCCGTAGTACAGGCGCTACAGCCAATACAGCGTTTATGATCGACGGTAATACAGCCTTCTTTctgct                                            <  1:297658/130‑1 (MQ=255)
                                                                                                                           cATAACACACATTTCGAGGATTTTTTACTTTCGGTATTTACGGTGGCCATCATCCACGGGCATGCCGTAGTACAGGCGCTACAGCCAATACAGCGTTTATGATCGACGGTAATACAGCCTTCTTTCTGCTGCCAGGTAATCGCa                              >  3:307970/1‑144 (MQ=255)
                                                                                                                           cATAACACACATTTCGAGGATTTTTTACTTTCGGTATTTACGGTGGCCATCATCCACGGGCATGCCGTAGTACAGGCGCTACAGCCAATACAGCGTTTATGATCGACGGTAATACAGCCTTCTTTCTGCTGCCAGGTAATCGCa                              <  4:307970/144‑1 (MQ=255)
                                                                                                                           cATAACACACATTTCGAGGATTTTTTACTTTCGGTATTTACGGTGGCCATCATCCACGGGCATGCCGTAGTACAGGCGCTACAGCCAATACAGCGTTTATGATCGACGGTAATACAGCCTTCTTTCTGCTGCCAGGTAATCGCACCAAt                         <  3:95022/149‑1 (MQ=255)
                                                                                                                           cATAACACACATTTCGAGGATTTTTTACTTTCGGTATTTACGGTGGCCATCATCCACGGGCATGCCGTAGTACAGGCGCTACAGCAAATACAGCGTTTATGATCGACGGTAATACAGCCTTCTTTCTCCTGCCAGGTAATCGCACCAAt                         >  5:362919/1‑149 (MQ=255)
                                                                                                                            aTAACACACATTTCGAGGATTTTTTACTTTCGGTATTTACGGTGGCCATCATCCACGGGCATGCCGTAGTACAGGCGCTACAGCCAATACAGCGTTTATGATCGACGGTAATACAGCCTTCTTTCTGCTGCCAGGTAATCGCACCAATc                        <  5:231002/149‑1 (MQ=255)
                                                                                                                                cacacaTTTCGAGGATTTTTTACTTTCGGTATTTACGGTGGCCATCATCCACGGGCATGCCGTAGTACAGGCGCTACAGCCAATACAGCGTTTATGATCGACGGTAATACAGCCTTCTTTCTGCTGCCAGGTAATCGCACCAATCGGGc                    <  1:379055/149‑1 (MQ=255)
                                                                                                                                cacacaTTTCGAGGATTTTTTACTTTCGGTATTTACGGTGGCCATCATCCACGGGCATGCCGTAGTACAGGCGCTACAGCCAATACAGCGTTTATGATCGACGGTAATACAGCCTTCTTTCTGCTGCCAGGTAATCGCACCAATCGGGc                    <  7:140554/149‑1 (MQ=255)
                                                                                                                                cacacaTTTCGAGGATTTTTTACTTTCGGTATTTACGGTGGCCATCATCCACGGGCATGCCGTAGTACAGGCGCTACAGCCAATACAGCGTTTATGATCGACGGTAATACAGCCTTCTTTCTGCTGCCAGGTAAACGCACCAATCgcg                     >  3:352373/1‑146 (MQ=255)
                                                                                                                                        tCGAGGATTTTCTACTTTCGGTATTTACGGTGGCCATCATCCACGGGCATGCCGTAGTACAGGCGCTACAGCCAATACAGCGTTTATGATCGACGGTAATACAGCCTTCTTTCTGCTGCCAGGTAATCGCACCAATCGGGCAGACGtt             <  3:308495/148‑1 (MQ=255)
                                                                                                                                          gAGGATTTTTTAATTTCGGTATTTACGGTGGCCATCATCCACGGGCATGCCGTAGTACAGGCGCTACAGCCAATACAGCGTTTATGATCGACGGTAATACAGCCTTCTTTCTGCTGCCAGGTAATCGCACCAATCGGGCAGACGTTCAt          <  6:374430/149‑1 (MQ=255)
                                                                                                                                           aGGATTTTTTACTTTCGGTATTTACGGTGGCCATCATCCACGGGCATGCCGTAGTACAGGCGCTACAGCCAATACAGCGTTTATGATCGACGGTAATACAGCCTTCTTTCTGCTGCCAGGTAATCGCACCAATCGGGCAGACGTTCATg         >  7:77143/1‑149 (MQ=255)
                                                                                                                                               atttttACTTTCGGTATTTACGCTGGCCATCATCCACGGGCATGCCGTAGTACAGGCGCTACAGCCAATACAGCGTTTATGATCGACGGTAATACAGCCTTCTTTCTGCTGCCAGGTAATCGCACCAATCGGGCAGACGTTCATGCAtt     <  1:61804/148‑1 (MQ=255)
                                                                                                                                                  tttACTTTCGGTATTTACGGTGGCCATCATCCACGGGCATGCCGTAGTACAGGCGCTACAGTCAATACAGCGTTTATGATCGACGGTAATACAGCCTTCTTTCTGCTGCCAGGTAATCGCACCAATCGGGCAGACGTTCATGCATTGCg  >  3:166904/1‑149 (MQ=255)
                                                                                                                                                  tttACTTTAGGTATTTACGGTTGCCATCATCCACGGGCATGCCGTAGTACAGGCGATAGAGCCAATACAGCGTTTATGTTCGACGGTAATACAGCCTTCTTTCTGCTGCAATGTAATCGCACCAATCGTGCAGACGTTCATGCATTGc   <  1:356824/148‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
TCTTAATATATTACCTGTCCAACCGTTAGCCATGATTTTTTCCTTTGCAAGATTCACACAGTAATATCTTTCCACTCGATAATTTTTAACGCCCCTGTTGGGCAGGCGTTTGCGCATTCACCGCATAACACACATTTCGAGGATTTTTTACTTTCGGTATTTACGGTGGCCATCATCCACGGGCATGCCGTAGTACAGGCGCTACAGCCAATACAGCGTTTATGATCGACGGTAATACAGCCTTCTTTCTGCTGCCAGGTAATCGCACCAATCGGGCAGACGTTCATGCATTGCG  >  NC_000913/1753798‑1754092

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑