| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 1,708,930 | C→A | 20.7% | P671Q (CCG→CAG) | rsxC → | SoxR iron‑sulfur cluster reduction factor component; putative membrane‑associated NADH oxidoreductase of electron transport complex |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,708,930 | 0 | C | A | 20.7% | 53.3 / 2.2 | 35 | P671Q (CCG→CAG) | rsxC | SoxR iron‑sulfur cluster reduction factor component; putative membrane‑associated NADH oxidoreductase of electron transport complex |
| Reads supporting (aligned to +/- strand): ref base C (18/9); new base A (5/3); total (23/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCGGTACCAGAAGAACAGGTTGATCCGCGCAAAGCGGCAGTT > NC_000913/1708791‑1709075 | gCCTCTATTTCCCGTGCCAAAGCACGCAATCTGTCTCAGCAAAAGGCTAATGCCGTGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGc < 7:55451/149‑1 (MQ=17) gCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAgaagaa > 7:74603/1‑147 (MQ=255) gCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTAGATCCGCGCAAAGCGGCAGTCGAAGCCGCTATTGCCCGCGCGCAAGCCCACAAAGGCGCACAGCACCCGGGTTAAGCCGAGCAAGATCAGCAGGTTGAGCCgcgc > 6:144033/1‑148 (MQ=9) aCAGGCTAATGCCGAGCCAGAAAAACAGGTCGATCCGCGCAAAGCCGCAGTAGAAGCCGCTATTGCCCGAGCGAAAGCAATCAAACCCAAACAGAAAAAGGCAAATTCGGAGGAGGAAGAAAAAGCTCATACGAGCGAAGCTGCAGtg > 2:33013/1‑147 (MQ=2) ccGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTAtt < 8:74603/149‑1 (MQ=255) cGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGCGCCAAAGCGCGCAAACGGGACCAGCAACAGGCTAATGCGGAGCCAGAAGACCAGGTTGATCCGCGCACAGCTGCAGTAGACGCGGCGATTg > 3:139761/1‑149 (MQ=14) ccAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAGCGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGCGTGCTCCGCGCCACGCTGCCGACGAAGCGTCTAATCCAcg > 1:34144/1‑149 (MQ=14) aGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAACGCGAGCAAACGCGAACAGCAACCGGATAATGCGGAGCCAGAAGAACAGGTTCATCCGCGCAAAGCTGACGTCGCCGCTGCTCTTGCCcgcg > 5:199526/1‑149 (MQ=14) aGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACTGGAACAGCAACCGGCTaa > 2:77787/1‑87 (MQ=255) aGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACTGGAACAGCAACCGGCTaa < 1:77787/87‑1 (MQ=255) gaagaaCAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCag > 2:355247/1‑97 (MQ=255) gaagaaCAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCag < 1:355247/97‑1 (MQ=255) aacaGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAGCGGGAACAGCAACAGGCTAATGCGGAGCCAGAAGAACAGGTTGAt > 1:44467/1‑107 (MQ=255) aacaGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACTGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGAt < 2:44467/107‑1 (MQ=255) aacaGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGc > 5:243779/1‑79 (MQ=255) aacaGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGc < 6:243779/79‑1 (MQ=255) acaGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGACCAGGGTGCTGCGCGCAACGCTCCCGGCGCCGCGGCGATTGCTCGCGGCagcc > 5:185323/1‑146 (MQ=17) aGGTCGATCCGCGCAAAGCCGCCGTAGAAGCCGCTATTGCCCGAGCAAAAGCAAGCAAACAGGAACAGCAACAGGCTAATGCGGATCCAGAAGATCAGGATGATCCGCGCAAAGAGGCAGTAGAAGAAGCTATTGCCCgcgcgcagggc > 4:230477/1‑142 (MQ=11) gcgcAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGGTAGGGACCGGCCCAAAGCACgaactcag > 3:74203/1‑141 (MQ=14) cAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTAGACGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGaa > 8:7114/1‑148 (MQ=255) aaaGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGaa > 8:279026/1‑147 (MQ=255) cgccgTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGACGTCGGAGCAGCTATTGCACGCGCCAAAGGAAGCAAGCGTGAACAGCaag > 2:383730/1‑148 (MQ=17) gccgTCGACCCCGCTATCGCCCGATCCACCGCGCTCACCCGGTAACAGCACCCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCAACa < 3:13206/149‑1 (MQ=17) gccgTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGACGCGGCTATTGCACGCGCCCAAGCACCCAACCGCGAACAGCaa > 7:386683/1‑147 (MQ=17) cttttaaGCAGCTATTGCCCTCGCCAAAGCCCGCACGCGTTCACAGCAACAGGCTACTGCGGCCCCAGACGATCTGGTCGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCAACAgg < 2:202530/144‑1 (MQ=11) gCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCGGCAGTCGAAGCGGCTATTGCCCGCGCAAAGGCCCGCTAGCTGGAGCCGCGTCAGGGGAATGc > 8:324577/1‑149 (MQ=17) aGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCGGTACCAGAAGAAc < 7:279026/149‑1 (MQ=255) cAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCCCGCGCAAAAGCCCGCAAGCAGGACCAGCAACACGCGAAGGCGGAACCAGAACAACAgg > 3:215981/1‑149 (MQ=21) aaaGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCAGTAGAAGCGGCTATTGCACGCGCGAAAGCAATCAAGCTGGAACAGCAACAGGCTAATGCGCTACCAGAAGAACAGGt > 3:170245/1‑149 (MQ=18) aaGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTAt < 2:375500/85‑1 (MQ=11) tggAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTAGATCCGCGCAAAGCAGCCGTCGAAGCGGCTATTGCAcgcg > 4:271688/2‑81 (MQ=11) aaccgcaaccgcCTAATGCGGAGCCAGAACACCCGGGTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCGGTACCAGAAGAACAGGTTGATCCGCGCAAAGCg < 1:383730/149‑1 (MQ=17) cAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTgg > 1:157448/1‑91 (MQ=255) cAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTgg < 2:157448/91‑1 (MQ=255) cAACAGGCTAATGCGGAGCCAGAAGAACAGGTCGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCAc < 5:298596/82‑1 (MQ=255) aaCCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCAAAAGCACGCAAGCTGGAACAGCAACAGGCTAAAGCGCTACCAGAAGAACAGGTTGATCCGCGCCAAGAGGCAGtt > 1:201915/1‑149 (MQ=18) | GCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCGGTACCAGAAGAACAGGTTGATCCGCGCAAAGCGGCAGTT > NC_000913/1708791‑1709075 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |