Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,458,164 A→G 6.1% Q223R (CAG→CGG)  paaF → 2,3‑dehydroadipyl‑CoA hydratase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,458,1640AG 6.1% 248.9 / 3.9 81Q223R (CAG→CGG) paaF2,3‑dehydroadipyl‑CoA hydratase
Reads supporting (aligned to +/- strand):  ref base A (37/39);  new base G (3/2);  total (40/41)
Fisher's exact test for biased strand distribution p-value = 6.75e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGACGTCTTCCCCAGCGATTTAACCCTCGAATACGCCTTACAGCTGGCATCGAAAATGGCACGTCACTCGCCGCTGGCCTTACAAGCGGCAAAGCAAGCGCTGCGCCAGTCGCAGGAAGTGGCTTTGCAAGCCGGACTTGCCCAGGAGCGACAGTTATTCACCTTGCTGGCGGCAACAGAAGATCGTCATGAAGGCATCTCCGCTTTCTTACAAAAACGCACGCCCGACTTTAAAGGACGCTAATGATGGAATTCATCCTCAGTCATGTAGAAAAGGGCGTGATGACAC  >  NC_000913/1458021‑1458309
                                                                                                                                               |                                                                                                                                                 
cGACGTCTTCCCCAGCGATTTAACCCTCGAATACGCCTTACAGCTGGCATCGAAAATGGCACGTCACTCGCCGCTGGCCTTACAAGCGGCAAAGCAAGCGCTGCGCCAGTCGCAGGAAGTGGCTTTGCAAGCCGGACTTGCCCAGGAGc                                                                                                                                              >  1:232903/1‑149 (MQ=255)
  aCGTCTTCCCCAGCGATTTAACCCTCGAATACGCCTTACAGCTGGCATCGAAAATGGCACGTCACTCGCCGCTGGCCTTACAAGCGGCAAAGCAAGCGCTGCGCCAGTCGCAGGAAGTGGCTTTGCAAGCCGGACTTGCCCAGGAGCGa                                                                                                                                            >  1:193534/1‑149 (MQ=255)
  aCGTCTTCCCCAGCGATTTAACCCTCGAATACGCCTTACAGCTGGCATCGAAAATGGCACGTCACTCGCCGCTGGCCTTACAAGCGGCAAAGCAAGCGCTGCGCCAGTCGCAGGAAGTGGCTTTGCAAGCCGGACTTGCCCAGGAGCGa                                                                                                                                            <  4:134205/149‑1 (MQ=255)
    gTCTTCCCCAGCGATTTAACCCTCGAATACGCCTTACAGCTGGCATCGAAAATGGCACGTCACTCGCCGCTGGCCTTACAAGCGGCAAAGCAAGCGCTGCGCCAGTCGCAGGAAGTGGCTTTGCAAGCCGGACTTGCCCAGGAGCGACa                                                                                                                                          <  8:175806/149‑1 (MQ=255)
      cTTCCCCAGCGATTTAACCCTCGAATACGCCTTACAGCTGGCATCGAAAATGGCACGTCACTCGCCGCTGGCCTTACAAGCGGCAAAGCAAGCGCTGCGCCAGTCGCAGGAAGTGGCTTTGCAAGCCGGACTTGCCCAGGAGCGACAGt                                                                                                                                        >  1:36369/1‑149 (MQ=255)
           ccAGCGATTTAACCCTCGAATACGCCTTACAGCTGGCATCGAAAATGGCACGTCACTCGCCGCTGGCCTTACAAGCGGCAAAGCAAGCGCTGCGCCAGTCGCAGGAAGTGGCTTTGCAAGCCGGACTTGCCCAGGAGCGACAGTTAtt                                                                                                                                    >  2:16374/1‑148 (MQ=255)
              gCGATTTAACCCTCGAATACGCCTTACAGCTGGCATCGAAAATGGCACGTCACTCGCCGCTGGCCTTACAAGCGGCAAAGCAAGCGCTGCGCCAGTCGCAGGAAGTGGCTTTGCAAGCCGGACTTGCCCAGGAGCGACAGTTATTCAcc                                                                                                                                <  6:233043/149‑1 (MQ=255)
              gCGATTTAACCCTCGAATACGCCTTACAGCTGGCATCGAAAATGGCACGTCACTCGCCGCTGGCCTTACAAGCGGCAAAGCAAGCGCTGCGCCAGTCGCAGGAAGTGGCTTTGCAAGCCGGACTTGCCCAGGAGCGACAGTTATTCAcc                                                                                                                                <  4:131722/149‑1 (MQ=255)
                  tttAACCCTCGAATACGCCTTACAGCTGGCATCGAAAATGGCACGTCACTCGCCGCTGGCCTTACAAGCGGCAAAGCAAGCGCTGCGCCAGTCGCAGGAAGTGGCTTTGCAAGCCGGACTTGCCCAGGAGCGACAGTTATTCACCTTGc                                                                                                                            <  4:194196/149‑1 (MQ=255)
                     aaCCCTCGAATACGCCTTACAGCTGGCATCGAAAATGGCACGTCACTCGCCGCTGGCCTTACAAGCGGCAAAGCAAGCGCTGCGCCAGTCGCAGGAAGTGGCTTTGCAAGCCGGACTTGCCCAGGAGCGACAGTTATTCACCTTGCTgg                                                                                                                         >  5:73362/1‑149 (MQ=255)
                       cccTCGAATACGCCTTACAGCTGGCATCGAAAATGGCACGTCACTCGCAGCTTTCCTTACAAGAGGCAAAGCAAGAGCTGCGCCAGTCGCAGGAAGTGGCTTTGCAAGCCGGACTAGCCAGGGAGCGACACTTATTCACCTTGCTggcg                                                                                                                       >  5:72245/1‑149 (MQ=255)
                         cTCGAATACGCCTTACAGCTGGCATCGAAAATGGCACGTCACTCGCCGCTGGCCTTACAAGCGGCAAAGCAAGCGCTGCGCCAGTCGCAGGAAGTGGCTTTGCAAGCCGGACTTGCCCAGGAGCGACAGTTATTCACCTTGCTggcgg                                                                                                                      >  4:294821/1‑148 (MQ=255)
                         cTCGAATACGCCTTACAGCTGGCATCGAAAATGGCACGTCACTCGCCGCTGGCCTTACAAGCGGCAAAGCAAGCGCTGCGCCAGTCGCAGGAAGTGGCTTTGCAAGCCGGACTTGCCCAGGAGCGACAGTTATTCACCTTGCTggcgg                                                                                                                      >  7:351139/1‑148 (MQ=255)
                          tCGAATACGCCTTACAGCTGGCATCGAAAATGGCACGTCACTCGCCGCTGGCCTTACAAGCGGCAAAGCAAGCGCTGCGCCAGTCGCAGGAAGTGGCTTTGCAAGCCGGACTTGCCCAGGAGCGACAGTTATTCACCTTGCTggcggc                                                                                                                     >  6:370277/1‑148 (MQ=255)
                          tCGAATACGCCTTACAGCTGGCATCGAAAATGGCACGTCACTCGCCGCTGGCCTTACAAGCGGCAAAGCAAGCGCTGCGCCAGTCGCAGGAAGTGGCTTTGCAAGCCGGACTTGCCCAGGAGCGACAGTTATTCACCTTGCTggcggc                                                                                                                     >  7:384560/1‑148 (MQ=255)
                                aCGACTTACAGCTGCCAGCCAAAATGGCACGTCACTCGCCGCTGGCCATACAAGCGCCAAAGCAAGCGCTGCGCCAGTCGCAGGAAGTGGCTTTGCAAGCCCGACATGCCCAGGAGCGCCAGTTATTCACCGTCCTGGCGGCAACag                                                                                                                >  4:328460/1‑147 (MQ=255)
                                  gCCTTACAGCTGGCATCGAACATGGCACGTCACTCGCCGCTGGCCTTACAAGCGGCAAAGGATGCGCTGCGCCAGTCGCAGGAAGTGGCTTTGCAAGCCGGACTTGCCCGGGAGCGACAGTTATTCACCTTGCTGGCGGCAACagaaga                                                                                                            <  6:72245/149‑1 (MQ=255)
                                  gCCTTACAGCTGGCATCGAAAATGGCACGTCACTCGCCGCTGGCCTTACAAGCGGCAAAGCAAGCGCTGCGCCAGTCGCAGGAAGTGGCTTTGCAAGCCGGACTTGCCCAGGAGCGACAGTTATTCACCTTGCTGGCGGCAACag                                                                                                                >  6:96844/1‑145 (MQ=255)
                                   ccTTACAGCTGGCATCGAAAATGGCACGTCACTCGCCGCTGGCCTTACAAGCGGCAAAGCAAGCGCTGCGCCAGTCGCAGGAAGTGGCTTTGCAAGCCGGACTTGCCCAGGAGCGACAGTTATTCACCTTGCTGGCGGCAACAGAAGAt                                                                                                           <  5:34934/149‑1 (MQ=255)
                                    cTTACAGCTGGCATCGAAAATGGCACGTCACTCGCCGCTGGCCTTACAAGCGGCAAAGCAAGCGCTGCGCCAGTCGCAGGAAGTGGCTTTGCAAGCCGGACTTGCCCAGGAGCGACAGTTATTCACCTTGCTGGCGGCAACAGAAGATc                                                                                                          <  5:269389/149‑1 (MQ=255)
                                    cTTACAGCTGGCATCGAAAATGGCACGTCACTCGCCGCTGGCCTTACAAGCGGCAAAGCAAGCGCTGCGCCAGTCGCAGGAAGTGGCTTTGCAAGCCGGACTTGCCCAGGAGCGACAGTTATTCACCTTGCTGGCGGCAACAGAAGATc                                                                                                          <  3:294821/149‑1 (MQ=255)
                                     ttACAGCTGGCATCGAAAATGGCACGTCACTCGCCGCTGGCCTTACAAGCGGCAAAGCAAGCGCTGCGCCAGTCGCAGGAAGTGGCTTTGCAAGCCGGCCTTGCCCAGGAGCGACAGTTATTCACCTTGCTGGCGGCAACAGAAGATCg                                                                                                         >  4:60939/1‑149 (MQ=255)
                                     ttACAGCTGGCATCGAAAATGGCACGTCACTCGCCGCTGGCCTTACAAGCGGCAAAGCAAGCGCTGCGCCAGTCGCAGGAAGTGGCTTTGCAAGCCGGACTTGCCCAGGAGCGACAGTTATTCACCTTGCTGGCGGCAACAGAAGATCg                                                                                                         >  7:221992/1‑149 (MQ=255)
                                     ttACAGCTGGCATCGAAAATGGCACGTCACTCGCCGCTGGCCTTACAAGCGGCAAAGCAAGCGCTGCGCCAGTCGCAGGAAGTGGCTTTGCAAGCCGGACTTGCCCAGGAGCGACAGTTATTCACCTTGCTGGCGGCAACAGAAGATCg                                                                                                         >  7:273971/1‑149 (MQ=255)
                                        cAGCTGGCATCGAAAATGGCACGTCACTCGCCGCTGGCCTTACAAGCGGCAAAGCAAGCGCTGCGCCAGTCGCAGGAAGTGGCTTTGCAAGCCGGACTTGCCCAGGAGCGACAGTTATTCACCTTGCTGGCGGCAACAGAAGATCGTc                                                                                                       <  2:387054/148‑1 (MQ=255)
                                        cAGCTGGCATCGAAAATGGCACGTCACTCGCCGCTGGCCTTACAAGCGGCAAAGCAAGCGCTGCGCCAGGCGCAGGAAGTGGCTTTGCAAGCCGGACTTGCCCAGGAGCGACAGTTATTCACCTTGCTGGCGGCAACAGAAGATCGTc                                                                                                       <  7:80773/148‑1 (MQ=255)
                                           cTGGCATCGAAAATGGCACGTCACTCGCCGCTGGCCTTACAAGCGGCAAAGCAAGCGCTGCGCCAGTCGCAGGAAGTGGCTTTGCAAGCCGGACTTGCCCAGGAGCGACAGTTa                                                                                                                                      <  1:393125/114‑1 (MQ=255)
                                           cTGGCATCGAAAATGGCACGTCACTCGCCGCTGGCCTTACAAGCGGCAAAGCAAGCGCTGCGCCAGTCGCAGGAAGTGGCTTTGCAAGCCGGACTTGCCCAGGAGCGACAGTTa                                                                                                                                      >  2:393125/1‑114 (MQ=255)
                                           cTGGCATCGAAAATGGCACGTCACTCGCCGCTGGCCTTACAAGCGGCAAAGCAAGCGCTGCGCCAGTCGCAGGAAGTGGCTTTGCAAGCCGGACTTGCCCAGGAGCGACAGTTATTCACCTTGCTGGCGGCAACAGAAGATCGTCATg                                                                                                    >  3:148319/1‑148 (MQ=255)
                                           cTGGCATCGAAAATGGCACGTCACTCGCCGCTGGCCTTACAAGCGGCAAAGCAAGCGCTGCGCCAGTCGCAGGAAGTGGCTTAGCAAGCCGGACTAGCCCGGGAGCGACTGGTATTCACCTTGCTGGCGGCAACCGAAGCTCGTCATg                                                                                                    >  5:89534/1‑148 (MQ=255)
                                            tGGCATCGAAAATGGCACGTCACTCGCCGCTGGCCTTACAAGCGGCAAAGCAAGCGCTGCGCCAGTCGCAGGAAGTGGCTTTGCAAGCCGGACTTGCCCAGGAGCGACAGTTATTCACCTTGCTGGCGGCAACAGAAGATCGTCATg                                                                                                    >  6:303605/1‑147 (MQ=255)
                                              gCATCGAAAATGGCACGTCACTCGCCGCTGGCCTTACAAGCGGCAAAGCAAGCGCTGCGCCAGTCGCAGGAAGTGGCTTTGCAAGCCGGACTTGCCCAGGAGCGACAGTTATTCACCTTGCTGGCGGCAACAGAAGATCGTCATGAAgg                                                                                                <  2:232903/149‑1 (MQ=255)
                                               cATCGAAAATGGCACGGCACTCGCCGCTGGCCTTACAAGCGGCAAAGCAAGCGCTGCGCCAGTCGCAGGAAGTGGCTTTGCAAGCCGGACTTGCCCAGGAGCGACAGTTATTCACCTTGCTGGCGGCAACAGAAGATCGTCATGAAGGc                                                                                               <  6:77065/149‑1 (MQ=255)
                                                aTCGAAAATGGCACGTCACTCGCCGCTGGCCTTACAAGCGGCAAAGCAAGCGCTGCGCCAGTCGCAGGAAGTGGCTTTGCAAGCCGGACTTGCCCAGGAGCGACAGTTATTCACCTTGCTGGCGGCAACAGAAGATCGTCATGAAGGCa                                                                                              >  1:3204/1‑149 (MQ=255)
                                                    aaaaTGGCACGTCACTCGCCGCTGGCCTTACAAGCGGCAAAGCAAGCGCTGCGCCAGTCGCAGGAAGTGGCTTTGCAAGCCGGACTTGCCCAGGAGCGACAGTTATTCACCTTGCTGGCGGCAACAGAAGATCGTCATGAAGGCAtctc                                                                                          >  3:279767/1‑149 (MQ=255)
                                                       aTGGCACGTCACTCGCCGCTGGCCTTACAAGCGGCAAAGCAAGCGCTGCGCCAGTCGCAGGAAGTGGCTTTGCAAGCCGGACTTGCCCAGGAGCGACAGTTATTCACCTTGCTGGCGGCAACAGAAGATCGTCATGAAGGCATCTCCGc                                                                                       >  3:319384/1‑149 (MQ=255)
                                                                  cTCGCCGCTGGCCTTACAAGCGGCAAAGCAAGCGCTGCGCCAGTCGCAGGAAGTGGCTTTGCAAGCCGGACTTGCCCAGGAGCGACAGTTATTCACCTTGCTGGCGGCAACAGAAGATCGTCATGAAGGCATCTCCGCTTTCTTACaa                                                                             >  4:227586/1‑148 (MQ=255)
                                                                  cTCGCCGCTGGCCTTACAAGCGGCAAAGCAAGCGCTGCGCCAGTCGCAGGAAGTGGCTTTGCAAGCCGGACTTGCCCAGGAGCGACAGTTATTCACCTTGCTGGCGGCAACAGAAGATCGTCATGAAGGCATCTCCGCTTTCTTACaa                                                                             >  7:264662/1‑148 (MQ=255)
                                                                   tCGCCGCTGGCCTTACAAGCGGCAAAGCAAGCGCTGCGCCAGTCGCAGGAAGTGGCTTTGCAAGCCGGACTTGCCCAGGAGCGACAGTTATTCACCTTGCTGGCGGCAACAGAAGATCGTCATGAAGGCATCTCCGCTTTCtt                                                                                 >  6:24834/1‑143 (MQ=255)
                                                                   tCGCCGCTGGCCTTACAAGCGGCAAAGCAAGCGCTGCGCCAGTCGCAGGAAGTGGCTTTGCAAGCCGGACTTGCCCAGGAGCGACAGTTATTCACCTTGCTGGCGGCAACAGAAGATCGTCATGAAGGCATCTCCGCTTTCtt                                                                                 <  5:24834/143‑1 (MQ=255)
                                                                   tCGCCGCTGGCCTTACAAGCGGCAAAGCAAGCGCTGCGCCAGTCGCAGGAAGTGGCTTTGCAAGCCGGACTTGCCCAGGAGCGACAGTTATTCACCTTGCTGGCGGCAACAGAAGATCGTCATGAAGGCATCTCCGCTTTCTTACaaa                                                                            >  7:205660/1‑148 (MQ=255)
                                                                     gccgcTGGCCTTACAAGCGGCAAAGCAAGCGCTGCGCCAGTCGCAGGAAGTGGCTTTGCAAGCCGGACTTGCCCAGGAGCGACAGTTATTCACCTTGCTGGCGGCAACAGAAGATCGTCATGAAGGCATCTCCGCTTTCTTACAAAAac                                                                         <  2:193534/149‑1 (MQ=255)
                                                                     gccgcTGGCCTTACAAGCGGCAAAGCAAGCGCTGCGCCAGTCGCAGGAAGTGGCTTTGCAAGCCGGACTTGCCCAGGAGCGACAGTTATTCACCTTGCTGGCGGCAACAGAAGATCGTCATGAAGGCATCTCCGCTTTCTTACAAAAac                                                                         <  8:27769/149‑1 (MQ=255)
                                                                     gccgcTGGCCTTACAAGCGGCAAAGCAAGCGCTGCGCCAGTCGCAGGAAGTGGCTTTGCAAGCCGGACTTGCCCAGGAGCGACAGTTATTCACCTTGCTGGCGGCAACAGAAGATCGTCATGAAGGCATCTCCGCTTTCTTACAAAAac                                                                         <  2:3204/149‑1 (MQ=255)
                                                                     gccgcTGGCCTTACAAGCGGCAAAGCAAGCGCTGCGCCAGTATCATGAAGTGGCTTTGCAAGACGGACTTTCCCAGGAGCGACATTTATTCACCTTGCTGGCGGCAACAGAAGATCGTCATGAATGCATCTACGCTTTCTTACAAATac                                                                         <  4:139362/149‑1 (MQ=255)
                                                                            gCCTTACAAGCGGCAAAGCAAGCGCTGCGCCAGTCGCAGGAAGTGGCTTTGCAAGCCGGACTTGCCCAGGAGCGACAGTTATTCa                                                                                                                                  >  1:215958/1‑85 (MQ=255)
                                                                            gCCTTACAAGCGGCAAAGCAAGCGCTGCGCCAGTCGCAGGAAGTGGCTTTGCAAGCCGGACTTGCCCAGGAGCGACAGTTATTCa                                                                                                                                  <  2:215958/85‑1 (MQ=255)
                                                                              cTTACAAGCGGCAAAGCAAGCGCTGCGCCAGTCGCAGGAAGTGGCTTTGCAAGCCGGACTTGCCCAGGAGCGACAGTTATTCACCTTGCTGGCGGCAACAGAAGATCGTCATGAAGGCATCTCCGCTTTCTTACAAAAACGCACGccc                                                                 >  4:35857/1‑148 (MQ=255)
                                                                                 aCAAGCGGCAAAGCAAGCGCTGCGCCAGTCGCAGGAAGTGGCTTTGCAAGCCGGACTTGCCCAGGAGCGACAGTTATTCACCTTGCTGGCGGCAACAGAAGATCGTCATGAAGGCATCTCCGCTTTCTTACAAAAACGCACGCCCGACt                                                             >  2:35749/1‑149 (MQ=255)
                                                                                   aaGCGGCAAAGCAAGCGCTGCGCCAGTCGCAGGAAGTGGCTTTGCAAGCCGGACTTGCCCAGGAGCGACAGTTATTCACCTTGCTGGCGGCAACAGAAGATCGTCATGCAGGCATCTCCGCTATCTTACAAAAACGCACGCCCCACtt                                                            >  6:56233/1‑148 (MQ=255)
                                                                                   aaGCGGCAAAGCAAGCGCTGCGCCAGTCGCAGGAAGTGGCTTTGCAAGCCGGACTTGCCCAGGAGCGACAGTTATTCACCTTGCTGGCGGCAACAGAAGATCGTCATGAAGGCATCTCCGCTTTCTTAc                                                                               <  2:343281/129‑1 (MQ=255)
                                                                                   aaGCGGCAAAGCAAGCGCTGCGCCAGTCGCAGGAAGTGGCTTTGCAAGCCGGACTTGCCCAGGAGCGACAGTTATTCACCTTGCTGGCGGCAACAGAAGATCGTCATGAAGGCATCTCCGCTTTCTTAc                                                                               >  1:343281/1‑129 (MQ=255)
                                                                                   aaGCGGCAAAGCAAGCGCTGCGCCAGTCGCAGGAAGTGGCTTTGCAAGCCGGACTTGCCCAGGAGCGACAGTTATTCACCTTGCTGGCGGCAACAGAAGATCGTCATGAAGGCATCTCCGCTTTCTTACAAAACCGCACGCCCGACtt                                                            >  1:87241/1‑148 (MQ=255)
                                                                                       ggCAAAGCAAGCGCTGCGCCAGTCGCAGGAAGTGGCTTTGCAAGCCGGACTTGCCCAGGAGCGACAGTTATTCACCTTGCTGGCGGCAACAGAAGATCGTCATGAAGGCATCTCCGCTTTCTTACAAAAACGCACGCCCGACTTTaaa                                                        <  5:370277/148‑1 (MQ=255)
                                                                                         cAAAGCAAGCGCTGCGCCAGTCGCAGGAAGTGGCTTTGCAAGCCGGACTTGCCCAGGAGCGACAGTTATTCACCTTGCTGGCGGCAACAGAAGATCGTCATGAAGGCATCTCCGCTTTCTTACAAAAACGCACGCCCGACTTTAAAGGa                                                     >  5:382007/1‑149 (MQ=255)
                                                                                         cAAAGCAAGCGCTGCGCCAGTCGCAGGAAGTGGCTTTGCAAGCCGGACTTGCCCAGGAGCGACAGTTATTCACCTTGCTGGCGGCAACAGAAGATCGTCATGAAGGCATCTCCGCTTTCTTACAAAAACGCACGCCCGACTTTAAAGGa                                                     <  8:351139/149‑1 (MQ=255)
                                                                                                agcGCTGCGCCAGTCGCAGGAAGTGGCTTTGCAAGCCGGACTTGCCCAGGAGCGACAGTTATTCACCTTGCTGGCGGCAACAGAAGATCGTCATGAAGGCATCTCCGCTTTCTTACAAAAACGCACGCCCGACTTTAAAGGACGCTAat                                              <  8:384560/149‑1 (MQ=255)
                                                                                                 gcgcTGCGCCAGTCGCAGGAAGTGGCTTTGCAAGCCGGACTTGCCCAGGAGCGACAGTTATTCACCTTGCTGGCGGCAACAGAAGATCGTCATGAAGGCATCTCCGCTTTCTTACAAAAACGCACGCCCGACTTTAAAGGACGCTAat                                              >  1:349129/1‑148 (MQ=255)
                                                                                                 gcgcTGCGCCAGTAGCAGGAAGTGGCTTTGCAAGACGGACTTGCCCAGGAGCGACAGTTATTCACCTTGCTGGCGGCAACAGAAGATCGGCATGAAGGCATCTCCGCTTTCTTACAAAAACGCACGCCCGACTTTAAAGGACGCTAatg                                             <  3:190405/149‑1 (MQ=255)
                                                                                                    cTGCGCCAGTCGCAGGAAGTGGCTTTGCAAGCCGGACTTGCCCAGGAGCGACAGTTATTCACCTTGCTGGCGGCAACAGAAGATCGTCATGAAGGCATCTCCGCTTTCTTACAAAAACGCACGCCCGACTTTAAAGGACGCTAatgatg                                          <  8:314722/149‑1 (MQ=255)
                                                                                                    cTGCGCCAGTCGCAGGAAGTGGCTTTGCAAGCCGGACTTGCCCAGGAGCGACAGTTATTCACCATGCTGGCGGCAACAGAAGATCGTCATGAAGGCATCTCCGCTTTCTTACAAAAACGCACGCCCGACTTTAAAGGACGCTAatgatg                                          <  1:325824/149‑1 (MQ=255)
                                                                                                      gcgcCAGTCGCAGGAAGTGGCTTTGCAAGCCGGACTTGCCCAGGAGCGACAGTTATTCACCTTGCTGGCGGCAACAGAAGATCGTCATGAAGGCATCTCCGCTTTCTTACAAAAACGCACGCCCGACTTTAAAGGACGCTAATGATGGa                                        <  4:327595/149‑1 (MQ=255)
                                                                                                       cgcCAGTCGCAGGAAGTGGCTTTGCAAGCCGGACTTGCCCAGGAGCGACAGTTATTCACCTTGCTGGCGGCAACAGAAGATCGTCATGAAGGCATCTCCGCTTTCTTACAAAAACGCACGCCCGACTTTAAAGGACGCTAATGATGGaa                                       <  6:382007/149‑1 (MQ=255)
                                                                                                       cgcCAGTCGCAGGAAGTGGCTTTGCAAGCCGGACTTGCCCAGGAGCGACAGTTATTCACCTTGCTGGCGGCAACAGAAGATCGTCATGAAGGCATCTCCGCTTTCTTACAAAAACGCACGCCCGACTTTAAAGGACGCTAATGATGGa                                        <  7:75720/148‑1 (MQ=255)
                                                                                                        gcCAGTCGCAGGAAGTGGCGTTGCAAGACGGAATTGCCCATGAGCGACAGTTATTCACCTTGCTTTCGGCAACAGAAGTTCGTCATGAAGGCATCTCCGCTTTCTTACAAAAACGCACGCCCGACTTTAAAGGACGCTAATGATGGAAt                                      <  5:50879/149‑1 (MQ=255)
                                                                                                        gcCAGTAGCAGGAAGTGGCTTTGCAAGCCGGACTTGCCCAGGAGCGACAGTTATTCTCCTTGCTGGCGGCAACAGAAGATCGTCATGAAGGCATCTCCGCTTTCTTACAAAAACGCACGCCCGACTTTAAAGGACGCTAATGATGGAAt                                      <  5:56233/149‑1 (MQ=255)
                                                                                                          cAGTCGCAGGAAGTGTCTTTGCAAGGCGGACTTGCCGAGGAGCGACAGTTATTCACCTTTCTGGCGGCAACAGAAGATCGTc                                                                                                       <  8:265230/82‑1 (MQ=255)
                                                                                                          cAGTCGCAGGAAGTGGATTTGCAAGCAGGACTTGCCCAGGAGAGACAGTTATTCACCTTGCTGGCGGCAACACAAGATCGTc                                                                                                       >  7:265230/1‑82 (MQ=255)
                                                                                                               gCAGGAAGTGGCTTTGCAAGCCGGACTTGCCCAGGAGCGACAGTTATTCACCTTGCTGGCGGCATCAGAAGATCGTCATGAAGGCATATCCGCATTCTTACAAAAACCCACGCCCGACTATAAAGGACGCTAATGATGGAATTCATCCt                               >  3:350332/1‑149 (MQ=255)
                                                                                                               gCAGGAAGTGGAGTGGCATGCCGGACTTTCCCGTGTGCGTCAGGTATTCACCTTGCTGGCGGCAAAAGAAGAGCGTCATGAAGGCATCGCCGCTTTCTTACAAAAACGCACGCGAGAAGTTAAGGGTCTATAATGATGGAATTCGTcat                               >  2:277140/1‑147 (MQ=255)
                                                                                                                cAGGAAGTGGCTTTGCAAGCCGGACTTGCCCAGGAGCGACAGTTATTCACCTTGCTGGCGGCAACAGAAGATCGTCATGAAGGCATCTCCGCTTTCTTACAAAAACGCACGCCCGACTTTAAAGGACGCTAATGATGGAATTCATCCTc                              <  5:303605/149‑1 (MQ=255)
                                                                                                                  ggAAGTGGCTTTGCAAGCCGGACTTGCCCAGGAGCGACAGTTATTCACCTTGCTGGCGGCAACAGAAGATCGTCATGAAGGCATCTCCGCTTTCTTACAAAAACGCACGCCCGACTTTAAAGGACGCTAATGATGGAATTCATCCTCAg                            <  8:221992/149‑1 (MQ=255)
                                                                                                                    aaGTGGCTTTGCAAGCCGGACTTGCCCAGGAGCGACAGTTATTCACCTTGCTGGCGGCAACAGAAGATCGTCATGAAGGCATCTCCGCTTTCTTACAAAAACGCACGCCCGACTTTAAAGGACGCTAATGATGGAATTCATCCTCAGTc                          <  5:270849/149‑1 (MQ=255)
                                                                                                                      gTGGCTTTGCAAGCCGGACTTGCCCAGGAGCGACAGTTATTCAGCTTGCTGGCGGCAACAGAAGATCGTCTTGAAGGCATCTCCGCTTTCTTACAAAAACGCACGCCCGACTTTaa                                                         <  6:5079/116‑1 (MQ=255)
                                                                                                                      gTGGCTTTGCAAGCCGGACTTGCCCAGGAGCGACAGTTATTCACCTTGCTGGCGGCAACAGAAGATCGTCATGAAGGCATCTCCGCTTTCTTACAAAAACGCACGCCCGACTTTaa                                                         >  5:5079/1‑116 (MQ=255)
                                                                                                                       tGGCTTTGCAAGCCGGACTTGCCCAGGAGCGACAGTTATTCACCTTGCTGGCGGCAACAGAAGATCGTCATGAAGGCATCTCCGCTTTCTTACAAAAACGCACGCCCGACTTTAAAGGACGCTAATGATGGAATTCATCCTCAGTCATg                       >  6:152307/1‑149 (MQ=255)
                                                                                                                         gcgatGCTAGCCGGACTTGCCCGGGAGCGACAGTTATTCACCTTGCTGGCGGCATCAGAAGATCGTCATGAAGGCATCTCCGCTTTCTTACAAAAACGCACGCCCGACTTTAAAGGACGCTAATGATGGAATTCATCCTCAGTCATGt                      <  6:89534/144‑1 (MQ=255)
                                                                                                                                       aCTTGCCCAGGAGCGACAGTTATTCACCTTGCTGGCGGCAACAGAAGATCGTCATGAAGGCATCTCCGCTTTCTTACAAAAACGCACGCCCGACTTTAAAGGACGCTAATGATGGAATTCATCCTCAGTCATGTAGAAAAGGGCGtgat       <  4:319384/149‑1 (MQ=255)
                                                                                                                                           gCCCAGGAGCGACAGTTATTCACCTTGCTGGCGGCAACAGAAGATCGTCATGAAGGCATCTCCGCTTTCTTACAAAAACGCACGCCCGACTTTAAAGGACGCTAATGATGGAATTCATCCTCAGTCATGTAGAAAAGGGCGtgatga     <  5:96844/147‑1 (MQ=255)
                                                                                                                                           gCCCAGGAGCGACAGTTATTCACCTTGCTGGCGGCAACAGAAGATCGTCATGAAGGCATCTCCGCTTTCTTACAAAAACGCACGCCCGACTTTAAAGGACGCTAATGATGGAATTCATCCTCAGTCATGTAGAAAAGGGCGTGATGac    <  1:16374/148‑1 (MQ=255)
                                                                                                                                             ccAGGAGCGACAGTTATTCACCTTGCTGGCGGCAACAGAAGATCGTCATGAAGGCATCTCCGCTTTCTTACAAAAACGCACGCCCGACTTTAAAGGACGCTAATGATGGAATTAATCCTCAGTCATGTAGAAAAGGGCGTGATGacac  >  4:354184/1‑148 (MQ=255)
                                                                                                                                               |                                                                                                                                                 
CGACGTCTTCCCCAGCGATTTAACCCTCGAATACGCCTTACAGCTGGCATCGAAAATGGCACGTCACTCGCCGCTGGCCTTACAAGCGGCAAAGCAAGCGCTGCGCCAGTCGCAGGAAGTGGCTTTGCAAGCCGGACTTGCCCAGGAGCGACAGTTATTCACCTTGCTGGCGGCAACAGAAGATCGTCATGAAGGCATCTCCGCTTTCTTACAAAAACGCACGCCCGACTTTAAAGGACGCTAATGATGGAATTCATCCTCAGTCATGTAGAAAAGGGCGTGATGACAC  >  NC_000913/1458021‑1458309

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: