Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 520,163 C→T 5.5% P144L (CCG→CTG)  ybbA → putative ABC transporter ATPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913520,1630CT 5.5% 228.6 / 2.5 73P144L (CCG→CTG) ybbAputative ABC transporter ATPase
Reads supporting (aligned to +/- strand):  ref base C (38/30);  new base T (2/2);  total (41/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.59e-01

AGTCATTTATGTTAATTCCTACCCTTAACGCGCTGGAAAACGTCGAGCTTCCGGCTCTGCTGCGCGGTGAGAGTAGCGCGGAAAGTCGTAACGGGGCGAAAGCGTTGCTCGAACAGTTAGGGCTGGGTAAACGTCTGGATCATCTTCCGGCACAGCTTTCCGGCGGTGAACAGCAACGAGTGGCGCTGGCACGAGCCTTTAATGGTCGACCTGATGTGCTGTTTGCCGACGAACCCACCGGCAACCTTGACCGCCAGACGGGCGATAAAATTGCCGACCTGCTGTTTTCCCT  >  NC_000913/520016‑520307
                                                                                                                                                   |                                                                                                                                                
aggcATTTATGTTAATTCCTACCCTTATCTCTCTGGAAAAGGTCGAGCTTCCGGCTCTGCTGAGCTGTGATAGTAGCGCGGAAAGTATTAACGTGGCGAAAGCGGTGCTCGAACATTTAGGGCTGTGTAAACGTCTGGATCATCTTCCg                                                                                                                                                 <  7:282159/146‑1 (MQ=255)
aGTCATTTATGTTAATTCCTACCCTTAACGCGCTGGAAAACGTCGAGCTTCCGGCTCTGCTGCGCGGTGAGAGTAGCGCGGAAAGTCGTAACGGGGCGAAAGCGTTGCTCGAACAGTTAGGGCTGGGTAAACGTCTGGATCATCTTcc                                                                                                                                                  <  8:52148/148‑1 (MQ=255)
aGTCATTTATGTTAATTCCTACCCTTAACGCGCTGGAAAACGTCGAGCTTCCGGCTCTGCTGCGCGGTGAGAGTAGCGCGGAAAGTCGTAACGGGGAGAAAGCGTTGCTCGAACAGTTAGGGCTGGGTAAACGTCTGGATCATCTTCCg                                                                                                                                                 >  6:143401/1‑149 (MQ=255)
 gTCATTTATGTTAATTCCTACCCTTAACGCGCTGGAAAACGTCGAGCTTCCGGCTCTGCTGCGCGGTGAGAGTAGCGCGGAAAGTCGTAACGGGGCGAAAGCGTTGCTCGAACAGTTAGGGCTGGGTAAACGTCTGGATCATCTTCCgg                                                                                                                                                >  2:372378/1‑149 (MQ=255)
    aTTTATGTTAATTCCTACCCTTAACGCGCTGGAAAACGTCGAGCTTCCGGCTCTGCTGCGCGGTGAGAGTAGCGCGGAAAGTCGTAACGGGGCGAAAGCGTTGCTCGAACAGTTAGGGCTGGGTAAACGTCTGGATCATCTTCCGGca                                                                                                                                              <  2:102589/148‑1 (MQ=255)
    aTTTATGTTAATTCCTACCCTTAACGCGCTGGAAAACGTCGAGCTTCCGGCTCTGCTGCGCGGTGAGAGTAGAGCGGAAAGTCGTAACGGGGCGAAAGCGTTGCTCGAACAGTTAGGGCTGGGTAAACGTCTGGATCATCTTCCGGca                                                                                                                                              >  1:384220/1‑148 (MQ=255)
    aTTTATGTTAATTCCTACCCTTAACGCGCTGGAAAACGTCGAGCTTCCGGATCTGCTGAGCGGTGAGAGTAGCGCGGAAAGTCGTAACGGGGCGAAAGCGTTGCTCGAACAGTTAGGGCTGGGTAAACGTCTGGATCATCTTACGGca                                                                                                                                              >  5:55382/1‑148 (MQ=255)
    aTTTATGTTAATTCCTACCCTTAACGCGCTGGAAAACGTCGAGATTCCGGCTCTGCTGCGCGGTGAGAGTAGCTCGGAAAGTCGTAACGGGGCGAAAGCGTTGCTCGAACAGTTAGGGCTCGGTAAACGTCTGGATCATCTTCCGGca                                                                                                                                              >  5:104114/1‑148 (MQ=255)
       tATGTTAATTCCTACCCTTAACGCGCTGGAAAACGTCGAGCTTCCGGCTCTGCTGCGCGGTGAGAGTAGCGCGGAAAGTCGTAACGGGGCGAAAGCGTTGCTCGAACAGTTAGGGCTGGGTAAACGTCTGGATCATCTTCCGGCACAg                                                                                                                                           <  6:17895/148‑1 (MQ=255)
         tGTTAATTCCTACCCTTAACGCGCTGGAAAACGTCGAGCTTCCGGCTCTGCTGCGCGGTGAGAGTAGCGCGGAAAGTCGTAACGGGGCGAAAGCGTTGCTCGAACAGTTAGGGCTGGGTAAACGTCTGGATCATCTTCCGGCACAGCtt                                                                                                                                        >  4:158900/1‑149 (MQ=255)
                  cTACCCTTAACGCGCTGGAAAACGTCGAGCTTCCGGCTCTGCTGCGCGGTGAGAGTAGCGCGGAAAGTCGTAACGGGGCGAAAGCGTTGCTCGAACAGTTAGGGCTGGGTAAACGTCTGGATCATCTTCCGGCACAGCTTTCCGGCGGt                                                                                                                               <  3:175890/149‑1 (MQ=255)
                        ttAACGCGCTGGAAAACGTCGAGCTTCCGGCTCTGCTGCGCGGTGAGAGTAGCGCGGAAAGTCGTAACGGGGCGAAAGCGTTGCTCGAACAGTTAGGGCTGGGGAAACGTATGGATCATCTTCCGGCCCAGCTTTCCGGCGGTGAACAg                                                                                                                         >  2:171500/1‑149 (MQ=255)
                          aaCGCGCTGGAAAACGTCGAGCTTCCGGCTCTGCTGCGCGGTGAGAGTAGCGCGGAAAGTCGTAACGGGGCGAAAGCGTTGCTCGAACAGTTAGGGCTGGGTAAACGTCTGGATCATCTTCCGGCACAGCTTTCCGGCGGTGAACAGc                                                                                                                        >  3:332571/1‑148 (MQ=255)
                          aaCGCGCTGGAAAACGTCGAGCTTCCGGCTCTGCTGCGCGGTGAGAGTAGCGCGGAAAGTCGTAACGGGGCGAAAGCGTTGCTCGAACAGTTAGGGCTGGGTAAACGTCTGGATCATCTTCCGGCACAGATTTCCGGCGGTGAACAGc                                                                                                                        <  2:350248/148‑1 (MQ=255)
                           aCGCGCTGGAAAACGTCGAGCTTCCGGCTCTGCTGCGCGGTGAGAGTAGCGCGGAAAGTCGTAACGGGGCGAAAGCGGTGCTCGAACAGTTAGGGCTGGGTAAACGTCTGGATCATCTTCCGGCACAGCTTTCCGGCGGTGAACAGCaa                                                                                                                      <  4:266858/149‑1 (MQ=255)
                             gcgcTGGAAAACGTCGAGCTTCCGGCTCTGCTGCGAGGTGAGAGTAGCGCGGAAAGTCGTAACGGGGCGAAAGCGTTGCTCGAACAGTTAGGGCTGGGTAAACGTCTGGATCATCTTCCGGCACAGCTTTCCGGCGGTGAACAGCAAc                                                                                                                     <  2:150373/148‑1 (MQ=255)
                                 tGGAAAACGTCGAGCTTCCGGCTCTGCTGCGCGGTGAGAGTAGCGCGGAAAGTCGTAACGGGGCGAAAGCGTTGCTCGAACAGTTAGGGCTGGGTAAACGTCTGGATCATCTTCCGGCTCAGCTTTCCGGCGGTGAACAGCAACGAGTg                                                                                                                >  6:133568/1‑149 (MQ=255)
                                  ggAAAACGTCGAGCTTCCGGCTCTGCTGCGCGGTGAGAGTAGCGCGGAAAGTCGTAACGGGGCGAAAGCGTTGCTCGAACAGTTAGGGCTGGGTAAACGTCTGGATCATCTTCCGGCACAGCTTTCCGGCGGTGAACAGCAACGAGTgg                                                                                                               >  8:227212/1‑149 (MQ=255)
                                  ggAAAACGTCGAGCTTCCGGCTCTGCTGCGCGGTGAGAGTAGCGCGGAAAGTCGTAACGGGGCGAAAGCGTTGCTCGAACAGTTAGGGCGGGGTCAACGTCTGGATCATCTTCCGGCCCAGCTTTCCGGCGGTGAACCGCAACGAGTgg                                                                                                               >  2:328587/1‑149 (MQ=255)
                                   gAAAACGTCGAGCTTCCGGCTCTGCTGCGCGGTGAGAGTAGCGCGGAAAGTCGTAACGGGGCGAAAGCGTTGCTCGAACAGTTAGGGCTGGGTAAACGTCTGGATCATCTTCCGGCACAGCTTTCCGGCGGt                                                                                                                               >  1:199593/1‑132 (MQ=255)
                                   gAAAACGTCGAGCTTCCGGCTCTGCTGCGCGGTGAGAGTAGCGCGGAAAGTCGTAACGGGGCGAAAGCGTTGCTCGAACAGTTAGGGCTGGGTAAACGTCTGGATCATCTTCCGGCACAGCTTTCCGGCGGt                                                                                                                               <  2:199593/132‑1 (MQ=255)
                                   gAAAACGTCGAGCTTCCGGCTCTGCTGCGCGGTGAGAGTAGCGCGGAAAGTCGTAACGGGGCGAAAGCGTTGCTCGAACAGTTAGGGCTGGGTAAACGTCTGGATCATCTTCCGGCACAGCTTTCCGGCGGTGAACAGCAACGAGTg                                                                                                                <  2:308222/147‑1 (MQ=255)
                                         gTCGAGCTTCCGGCTCTGCTGCGCGGTGAGAGTAGCGCGGAAAGTCGTAACGGGGCGAAAGCGTTGCTCGAACAGTTAGGGCTGGGTAAACGTCTGGATCATCTTCCGGCACAGCTTTCCGGCGGTGAACAGCAACGAGTGGCGCTgg                                                                                                         >  4:314492/1‑148 (MQ=255)
                                         gTCGAGCTTCCGGCTCTGCTGCGCGGTGAGAGTAGCGCGGAAAGTCGTAACGGGGCGAAAGCGTTGCTCGAACAGTTAGGGCTGGGTAAACGTCTGGATCATCTTCCGGCACAGCTTTCCGGCGGTGAACAGCAACGAGTGGCGCTgg                                                                                                         >  7:49969/1‑148 (MQ=255)
                                          tCGAGCTTCCGGCTCTGCTGCGCGGTGAGAGTAGCGCGGAAAGTCGTAACGGGGCGAAAGCGTTGCTCGAACAGTTAGGGCTGGGTAAACGTCTGGATCATCTTCCGGCACAGCTTTCCGGCGGTGAACAGCAACGAGTGGCGCTGGCa                                                                                                       >  8:382655/1‑149 (MQ=255)
                                          tCGAGCTTCCGGCTCTGCTGCGCGGTGAGAGTAGCGCGGAAAGTCGTAACGGGGCGAAAGCGTTGCTCGAACAGTTAGGGCTGGGTAAACGTCTGGATCATCTTCCGGCACAGCTTTCCGGCGGTGAACAGCAACGAGTGGCGCTGGCa                                                                                                       <  3:314492/149‑1 (MQ=255)
                                            gAGCTTCCGGCTCTGGTGCGCGGTGAGAGTTGCGCGGAAAGTCGTAACGGGGCGAAAGCGTTGCTAGAACAGTTAGGGCGGGGTAATCGTCTGGATCATGTTCCGGCACAGCTTTCCGGCGGTGAACTGCAACGAGTGGCGCTGGCAc                                                                                                      <  6:55382/148‑1 (MQ=255)
                                            gAGCTTCCGGCTCTGCTGCGCGGTGAGAGTAGCGCGGAAAGTCGTAACGGGGCGAAAGCGTTGCTCGAACAGTTAGGGCTGGGTAAACGTCTGGATCATCTTCCGGCACAGCTTTCCGGCGGTGAACAGCAACGAGTGGCGCTGGCAc                                                                                                      <  2:212972/148‑1 (MQ=255)
                                            gAGCTTCCGGCGCTGCTGCGAGGTGAGAGTAGCGCGGAAAGTGGTAACGGGGCGAAAGCGTTGCTCGAACAGTTATGGCTGGGTAAACGTCTGGATCATCTTCCGGCACAGCTTTCCGGCGGTGAACAGCAACGAGTGGCGCTGGCAc                                                                                                      <  6:104114/148‑1 (MQ=255)
                                                   cGGCTCTGCTGCGCGGTGAGAGTAGCGCGGAAAGTCGTAACGGGGCGAAAGCGTTGCTCGAACAGTTAGGGCTGGGTAAACGTCTGGATCATCTTCCGGCACAGCTTTCCGGCGGTGAACAGCAACGAGTGGCGCTGGCACGAGCCtt                                                                                               <  6:123499/148‑1 (MQ=255)
                                                    ggCTCTGCTGCGCGGTGAGAGTAGCGCGGAAAGTCGTAACGGGGCGAAAGCGTTGCTCGAACAGTTAGGGCTGGGTAAACGTCTGGATCATCTTCCGGCACAGCTTTCCGGCGGTGAACAGCAACGAGTGGCGCTGGCACGAGCCTTTa                                                                                             <  8:207534/149‑1 (MQ=255)
                                                       tctGCTGCGCGGTGAGAGTAGCGCGGAAAGTCGTAACGGGGCGAAAGCGTTGCTCGAACAGTTAGGGCTGGGTAAACGTCTGGATCATCTTCCGGCACAGCTTTCCGGCGGTGAACAGCAACGAGTGGCGCTGGCACGAGCCTTTAATg                                                                                          >  7:246272/1‑149 (MQ=255)
                                                                cgGTGAGCGTAGCGCGGAAAGTCCTAACGGGGCGAAAGCGTTGCTCCAACAGTTAGGGCAGGGTAAACAGGAGGATCATCATCACGCACAGCTTTCCGGCGGTGAACAGCAACGAGTTGCGCAGGCACGAACCTTTAATGGGCGAc                                                                                    >  6:144555/1‑146 (MQ=255)
                                                                 ggTGAGAGTAGCGCGGAAAGTCGTAACGGGGCGAAAGCGTTGCTCGAACAGTTAGGGCTGGGTAAACGTCTGGATCATCTTCCGGCACAGCTTTCCGGCGGTGAACAGCAACGAGTGGCGCTGGCACGAGCCTTTAATGGTCGACCTGa                                                                                <  8:49969/149‑1 (MQ=255)
                                                                  gTGAGAGTAGCGCGGAAAGTCGTAACGGGGCGAAAGCGTTGCTCGAACAGTTAGGGCTGGGTAAACGTCTGGATCATCTTCCGGCTCAGCTTTCCGGCGGTGAACAGCAACGAGTGGCGCTGGCACGAGCCTTTAATGGTCGACCTGAt                                                                               <  5:133568/149‑1 (MQ=255)
                                                                   tGAGAGTAGCGCGGAAAGTCGTAACGGGGCGAAAGCGTTGCTCGAACAGTTAGGGCTGGGTAAACGTCTGGATCATCTTCCGGCACAGCTTTCCGGCGGTGAACAGCAACGAGTGGCGCTGGCACGAGCCTTTAATGGTCGCCCTGAtg                                                                              >  8:200992/1‑149 (MQ=255)
                                                                       agTAGCGCGGAAAGTCGTAACGGGGCGAAAGCGTTGCTCGAACAGTTAGGGCTGGGTAAACGTCTGGATCATCTTCCGGCACAGCTTTCCGGCGGTGAACAGCAACGAGTGGCGCTGGCACGAGCCTTTAATGGTCGACCTGATGTGCt                                                                          >  3:248113/1‑149 (MQ=255)
                                                                           gcgcGGAAAGTCGTATCGGGGCGAAAGCGTTGCTCGAACAGTTAGGGCTGGGTAAACGTCTGGATCATCTTCCGGCACAGCTTTCCGGCGGTGAACAGCAACGAGTGGCGCTGGCACGAGCCTTTAATGGTCGACCTGATGTGCTGttt                                                                      <  2:14727/149‑1 (MQ=255)
                                                                                     tCGTAACGGGGCGAAAGCGTTGCTCGAACAGTTAGGGCTGGGTAAACGTCTGGATCATCTTCCGGCACAGCTTTCCGGCGGTGAACAGCAACGAGTGGCGCTGGCACGAGCCTTTAATGGTCGACCTGATGTGCTGTTTGCCGACGAAc                                                            >  5:176577/1‑149 (MQ=255)
                                                                                     tCGTAACGGGGCGAAAGCGTTGCTCGAACAGTTAGGGCTGGGTAAACGTCTGGATCATCTTCCGGCACAGCTTTCCGGCGGTGAACAGCAACGAGTGGCGCTGGCACGAGCCTTTAATGGTCGACCTGATGTGCTGTTTGCCGACGAAc                                                            <  1:372378/149‑1 (MQ=255)
                                                                                       gTAACGGGGCGAAAGCGTTGCTCGAACAGTTAGGGCTGGGTAAACGTCTGGATCATCTTCCgg                                                                                                                                                <  6:165959/63‑1 (MQ=255)
                                                                                       gTAACGGGGCGAAAGCGTTGCTCGAACAGTTAGGGCTGGGTAAACGTCTGGATCATCTTCCgg                                                                                                                                                >  5:165959/1‑63 (MQ=255)
                                                                                        tAACGGGGCGAAAGCGTTGCTCGAACAGTTAGGGCTGGGTAAACGTCTGGATCATCTTCCGGCACAGCTTTCCGGCGGTGAACAGCAACGAGTGGCGCTGGCACGAGCCTTTAATGGTCGACCTGATGTGCTGTTTGCCGACGAACCCa                                                         >  3:29148/1‑149 (MQ=255)
                                                                                          aCGGGGCGAAAGCGTTGCTCGAACAGTTAGGGCTGGGTAAACGTCTGGATCATCTTCCGGCACAGCTTTCCGGCGGTGAACAGCAACGAGTGGCGCTGGCACGAGCCTTTAATGGTCGACCTGATGTGCTGTTTGCCGACGAACCCAcc                                                       >  4:96437/1‑149 (MQ=255)
                                                                                           cGGGGCGAAAGCGTTGCTCGAACAGTTAGGGCTGGGTAAACGTCTGGATCATCTTCCGGCACAGCTTTCCGGCGGTGAACAGCAACGAGTGGCGCTGGCACGAGCCTTTAATGGTCGACCTGATGTGCTGTTTGCCGACGAACCCACCg                                                      <  4:248113/149‑1 (MQ=255)
                                                                                           cGGGGCGAAAGCGTTGCTCGAACAGTTAGGGCTGGGTAAACGTCTGGATCATCTTCCGGCACAGCTTTCCGGCGGTGAACAGCAACGAGTGGCGCTGGCACGAGCCTTTAATGGTCGACCTGATGTGCTGTTTGCCGACGAACCCACCg                                                      <  4:332571/149‑1 (MQ=255)
                                                                                              ggCGAAAGCGTTGCTCGAACAGTTAGGGCTGGGTAAACGTCTGGATCATCTTCCGGCACAGCTTTCCGGCGGTGAACAGCAACGAGTGGCGCTGGCACGAGCCTTTAATGGTCGACCTCATGTGATGTTTGCCGACGAACACACCGGc                                                    >  2:303531/1‑148 (MQ=255)
                                                                                                 gAAAGCGTTGCTCGAACAGTTAGGGCTGGGTAAACGTCTGGATCATCTTCCGGCACAGCTTTCCGGCGGTGAACAGCAACGAGTGGCGCTGGCACGAGCCTTTAATGGTCGACCTGATGTGCTGTTTGCCGACGAACCCACCGGCAAcc                                                >  2:69836/1‑149 (MQ=255)
                                                                                                          gCTCGAACAGTTAGGGCTGGGTAAACGTCTGGATCATCTTCCGGCACAGCTTTCCGGCGGTGAACAGCAACGAGTGGCGCTGGCACGAGCCTTTAATGGTCGACCTGATGTGCTGTTTGCcgac                                                                >  4:339486/1‑124 (MQ=255)
                                                                                                          gCTCGAACAGTTAGGGCTGGGTAAACGTCTGGATCATCTTCCGGCACAGCTTTCCGGCGGTGAACAGCAACGAGTGGCGCTGGCACGAGCCTTTAATGGTCGACCTGATGTGCTGTTTGCcgac                                                                <  3:339486/124‑1 (MQ=255)
                                                                                                              gAACAGTTAGGGCTGGGTAAACGTCTGGATCATCTTCCGGCACAGCTTTCCGGCGGTGAACAGCAACGAGTGGCGCTGGCACGAGCCTTTAATGGTCGACCTGATGTGCTGTTTGCCGACGAACCCACCGGCACCCTTGACCGCCAGAc                                   >  3:89700/1‑149 (MQ=255)
                                                                                                                aCAGTTAGGGCTGGGTAAACGTCTGGATCATCTTCCGGCACAGCTTTCCGGCGGTGAACAGCAACGAGTGGCGCTGGCACGAGCCTTTAATGGTCGACCTGATGTGCTGTTTGCCGACGAACCCACCGGCAACCTTGACCGCCAGACgg                                 <  5:143401/149‑1 (MQ=255)
                                                                                                                    ttAGGGCTGGGTAAACGTCTGGATCATCTTCCGGCACAGCTTTCCGGCGGTGAACAGCAACGAGTGGCGCTGGCACGAGCCTTTAATGGTCGACCTGATGTGCTGTTTGCCGACGAACCCACCGGCAACCTTGACCGCCAGACGGGCGa                             >  7:391864/1‑149 (MQ=255)
                                                                                                                          cTGGGTAAACGTCTGGATCATCTTCCGGCACAGCTTTCCGGCGGTGAACAGCAACGAGTGGCGCTGGCACGAGCCTTTAATGGTCGACCTGATGTGCTGTTTGCCGACGAACCCACCGCCAACCTTGACCGCCAGACGGGCGATAAAAt                       >  2:148449/1‑149 (MQ=255)
                                                                                                                              gTAAACGTCTGGATCATCTTCCGGCACAGCTTTCCGGCGGTGAACAGCAACGAGTGGCGCTGGCACGAGCCTTTAATGGTCGACCTGATGTGCTGTTTGCCGACGAACCCACCGGCAAc                                                 <  4:138197/119‑1 (MQ=255)
                                                                                                                              gTAAACGTCTGGATCATCTTCCGGCACAGCTTTCCGGCGGTGAACAGCAACGAGTGGCGCTGGCACGAGCCTTTAATGGTCGACCTGATGTGCTGTTTGCCGACGAACCCACCGGCAAc                                                 >  3:138197/1‑119 (MQ=255)
                                                                                                                              gTAAACGTCTGGATCATCTTCCGGCACAGCTTTCCGGCGGTGAACAGCAACGAGTGGCGCTGGCACGAGCCTTTAATGGTCGACCTGATGTGCTGTTTGCCGACGAACCCACCGGCAACCTTGACCGCCAGACGGGCGATAAAATTGcc                   >  5:363993/1‑149 (MQ=255)
                                                                                                                              gTAAACGTCTGGATCATCTTCCGGCACAGCTTTCCGGCGGTGAACAGCAACGAGTGGCGCTGGCACGAGCCTTTAATGGTCGACCTGATGTGCTGTTTGCCGACGAACCCACCGGCAACCTTGACCGCCAGACGGGCGATAAAATTGcc                   <  1:69836/149‑1 (MQ=255)
                                                                                                                                  aCGTCTGGATCATCTTCCGGCACAGCTTTCCGGCGGTGAACAGCAACGAGTGGCGCTGGCACGAGCCGTTAATGGTCGCCCTGATGTGCTGTTTGCCGACGAACCCACCGGCAACCTTGACCGCCAGACGGGCGATAAAATTGCCGAcc               >  2:27539/1‑149 (MQ=255)
                                                                                                                                    gTCTGGATCATCTTCCGGCACAGCTTTCCGGCGGTGAACAGCAACGAGTGGCGCTGGCACGAGCCTTTAATGGTCGACCTGATGTGCTGTTTGCCGACGAACCCACCGGCAACCTTGACCGCCAGACGGGCGATAAAATTGCCGACctg             >  1:90325/1‑149 (MQ=255)
                                                                                                                                        ggATCATCTTCCGGCACAGCTTTCCGGCGGTGAACAGCAACGAGTGGCGCTGGCACGAGCCTTTAATGGTCGACCTGATGTGCTGTTTGCCGACGAACCCACCGGCAACCTTGACCGCCAGACGGGCGATAAAATTGCCGACCTGCTGt         >  3:350537/1‑149 (MQ=255)
                                                                                                                                         gATCATCTTCTGGCACAGCTTTCCGGCGGTGAACAGCAACGAGTGGCGCTGGCACGAGCCTTTAATGGTCGACCTGATGTGCTGTTTGCCGACGAACCCCCCGGCAACCTTGACCGCCAGACGGGCGATAAAATTGCCGAc                >  1:224866/1‑141 (MQ=255)
                                                                                                                                         gATCATCTTCTGGCACAGCTTTCCGGCGGTGAACAGCAACGAGTGGCGCTGGCACGAGCCTTTAATGGTCGACCTGATGTGCTGTTTGCCGACGAACCCACCGGCAACCTTGACCGCCAGACGGGCGATAAAATTGCCGAc                >  1:218814/1‑141 (MQ=255)
                                                                                                                                         gATCATCTTCTGGCACAGCTTTCCGGCGGTGAACAGCAACGAGTGGCGCTGGCACGAGCCTTTAATGGTCGACCTGATGTGCTGTTTGCCGACGAACCCACCGGCAACCTTGACCGCCAGACGGGCGATAAAATTGCCGAc                <  2:218814/141‑1 (MQ=255)
                                                                                                                                         gATCATCTTCTGGCACAGCTTTCCGGCGGTGAACAGCAACGAGTGGCGCTGGCACGAGCCTTTAATGGTCGACCTGATGTGCTGTTTGCCGACGAACCCACCGGCAACCTTGACCGCCAGACGGGCGATAAAATTGCCGAc                <  2:224866/141‑1 (MQ=255)
                                                                                                                                         gATCATCTTCCGGCACAGCTTTCCGGCGGTGAACAGCAACGAGTGGCGCTGGCACGAGCCTTTAATGGTCGACCTGATGTGCTGTTTGCCGACGAACCCACCGGCAACCTTGACCGCCAGACGGGCGATAAAATTGCCGACCTGCTGtt        >  8:123119/1‑149 (MQ=255)
                                                                                                                                           tcatcTTCCGGCACAGCTTTCCGGCGGTGAACAGCAACGAGTGGCGCTGGCACGAGCCTTTAATGGTCGACCTGATGTGCTGTTTGCCGACGAACCCACCGGCAACCTTGACCGCCAGACGGGCGATAAAATTGCCGACCTGCTGtttt      >  2:46527/1‑149 (MQ=255)
                                                                                                                                           tcatcTTCCGGCACAGCTTTCCGGCGGTGAACAGCAACGAGTGGCGCTGGCACGAGCCTTTAATGGTCGACCTGATGTGCTGTTTGCCGACGAACCCACCGGCAACCTTGACCGCCAGACGGGCGATAAAATTGCCGACCTGCTGtttt      <  1:46527/149‑1 (MQ=255)
                                                                                                                                           tcatcTTCCGGCACAGCTTTCCGGCGGTGAACAGCAACGAGTGGCGCTGGCACGAGCCTTTAATGGTCGACCTGAGGTGCTGTTTGCCGACGAACCCACCGGCAACCTTGACCGCCAGACGGGCGATAAAATTGCCGACCTGCTGtttt      <  3:158900/149‑1 (MQ=255)
                                                                                                                                             atcTTCCGGCACAGCTTTCCGGCGGTGAACAGCAACGAGTGGCGCTGGCACGAGCCTTTAATGGTCGACCTGATGTGCTGTTTGCCGACGAACCCACCGGCAACCTTGACCGCCAGACGGGCGATAAAATTGCCGACCTGCTGTTTTcc    <  5:144555/149‑1 (MQ=255)
                                                                                                                                             atcTTCCGACACAGCTTTCCGGCGGTGAACACCAACGACTGGCGCTGGCACGAGCCTTTAATGGTCGACCTGATGTGCTGTTTGCCGACGAACCCACCGGCAACCTTGACCCCCAGCCGGGCGATAAAATTGCCGACCTCCTTTTTTcc    >  6:288943/1‑149 (MQ=255)
                                                                                                                                              gcTTCCGGCACAGCTTTCCGGCGGTGAACAGCAACGAGTGGCGCTGGCACGAGCCTTTAATGGTCGACCTGATGTGCTGTTTGCCGACGAACCCACCGGCAACCTTGACCGCCAGACGGGCGATAAAATTGCCGACCTGCTGTTTTccc   <  1:303531/148‑1 (MQ=255)
                                                                                                                                               cTTCCGGCACAGCTTTCCTGCGGTGAACAGCAACGAGTGGCGCTGGCACGAGCCTTTAATGGTCGACCTGATGTGCTGTTTGCCGACGAACCCACCGGCAACCTTGACCGCCAGACGGGCGATAAAATTGCCGACCTGCTGTTTTCCCt  >  6:364577/1‑149 (MQ=255)
                                                                                                                                               cTTCCGGCACAGCTTTCCGGCGGTGAACAGCAACGAGTGGCGCTGGCACGAGCCTTTAATGGTCGACCTGATGTGCTGTTTGCCGACGAACCCACCGGCAACCTTGACCGCCAGACGGGCGATAAAATTGCCGACCTGCTGTTTTCCCt  >  2:206684/1‑149 (MQ=255)
                                                                                                                                                  ccGGCACAGCTTTCCGGCGGTGAACAGCAACGAGTGGCGCTGGCACGAGCCTTTAATGGTCGACCTGATGTGCTGTTTGCCGACGAACCCACCGGCAACCTTGACCGCCAGACGGGCGATAAAATTGCCGACCTGCTGTTTTccc   <  4:29148/145‑1 (MQ=255)
                                                                                                                                                  ccGGCACAGCTTTCCGGCGGTGAACAGCAACGAGTGGCGCTGGCACGAGCCTTTAATGGTCGACCTGATGTGCTGTTTGCCGACGAACCCACCGGCAACCTTGACCGCCAGACGGGCGATAAAATTGCCGACCTGCTGTTTTccc   <  4:350537/145‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                
AGTCATTTATGTTAATTCCTACCCTTAACGCGCTGGAAAACGTCGAGCTTCCGGCTCTGCTGCGCGGTGAGAGTAGCGCGGAAAGTCGTAACGGGGCGAAAGCGTTGCTCGAACAGTTAGGGCTGGGTAAACGTCTGGATCATCTTCCGGCACAGCTTTCCGGCGGTGAACAGCAACGAGTGGCGCTGGCACGAGCCTTTAATGGTCGACCTGATGTGCTGTTTGCCGACGAACCCACCGGCAACCTTGACCGCCAGACGGGCGATAAAATTGCCGACCTGCTGTTTTCCCT  >  NC_000913/520016‑520307

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: