Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 508,748 C→A 5.2% intergenic (+189/+127) ybaQ → / ← copA putative DNA‑binding transcriptional regulator/copper transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913508,7480CA 5.2% 284.3 / 3.2 86intergenic (+189/+127)ybaQ/copAputative DNA‑binding transcriptional regulator/copper transporter
Reads supporting (aligned to +/- strand):  ref base C (44/37);  new base A (2/3);  total (46/40)
Fisher's exact test for biased strand distribution p-value = 6.60e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGGCATCCGAGCACCATTGCCTGATGCGACGCTTACCCGTCTTATCAGGCCTACAAACTTGTGCCTGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATCCGAGCACCATTGCCTGATGCGACGCTTACGCGTCTTATCAGGCCTACAAACCTGTGCCTGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTCATGCGTCGATGCCAAATGCGCCACCCTAAAGCAGCGCATCCGCAATGATGTACTTATTCCTTCGGTTTA  >  NC_000913/508612‑508890
                                                                                                                                        |                                                                                                                                              
cggcatccgAGCGCCATTGCCTGATGCGACGCTTACCCGTCTTATCAGGCCTACAAACTTGTGCCTGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATCCGAGCACCATTGCCTGATGCGACGCGTAAGCGTCGCATCa                                                                                                                                     <  1:195446/148‑1 (MQ=18)
cggcatccgAGCACCATTGCGTGATGAGTCGCTTACCCGTCTTAGCAGGGCTACAAACTTGTGCCTGTACCGTAGGCCGGATAAGGCGTTTACGCCTCATCCGGCATCCTAGCACCATTGCCTGATGCGACGCTTACGCGTCGTATCa                                                                                                                                     <  5:258540/148‑1 (MQ=18)
cggcatccgAGCACCATTGCCTGATGCGACGCTTACCCGTCTTATCAGGCCTACAAACTTGTGCCTGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATCCGAGCGCCATTGCCTGATGCGACGCTTACGCGTCTTATCAg                                                                                                                                    >  6:308297/1‑149 (MQ=255)
cggcatccgAGCACCATTGCCTGATGCGACGCTTACCCGTCTTATCAGGCCTACAAACTTGTGCCTGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATCCGAGCACCATTGCCTGATAAGACGCGTAAGCGTCGCATCAg                                                                                                                                    <  1:11874/149‑1 (MQ=17)
  gcatccgAGCACCATTGCCTGATGCGACGCTTACCCGTCTTATCAGGCCTACAAACTTGTGCCTGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATCCGAGCACCATTGCCTGATGCGACGCTTACGCGTCTTATCAGGc                                                                                                                                  <  1:127683/149‑1 (MQ=255)
  gcatccgAGCACCATTGCCTGATGCGACGCTTACCCGTCTTATCAGGCCTACAAACTTGTGCCTGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATCCGAGCACCATTGCCTGATGCGACGCTTACGCGTCTTATCAGGc                                                                                                                                  <  8:276171/149‑1 (MQ=255)
    atccgAGCACCATTGCCTGATGCGACGCTTACCCGTCTTATCAGGCCTACAAACTTGTGCCTGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATCCGAGCACCATTGCCTGATGCGACGCTTACGCGTCTTATCAGGcc                                                                                                                                 >  2:250906/1‑148 (MQ=255)
       cgAGCACCATTGCCTGATGCGACGCTTACCCGTCTTATCAGGCCTACAAACTTGTGCCTGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATCCGAGCACCATTGCCTGATGCGACGCTTACGCGTCTTATCAg                                                                                                                                    <  4:119092/142‑1 (MQ=255)
       cgAGCACCATTGCCTGATGCGACGCTTACCCGTCTTATCAGGCCTACAAACTTGTGCCTGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATCCGAGCACCATTGCCTGATGCGACGCTTACGCGTCTTATCAg                                                                                                                                    >  3:119092/1‑142 (MQ=255)
        gAGCACCATTGCCTGATGCGACGCTTACCCGTCTTATCAGGCCTACAAACTTGTGCCTGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATCCGAGCCCCAATGCCTGATGCGACGCTTACGCGTCTTATCAGGCCTACa                                                                                                                             >  5:172579/1‑148 (MQ=255)
        gAGCACCATTGCCTGATGCGACGCTTACCCGTCTTATCAGGCCTACAAACTAGTGCCTGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATCCAAGCACCAATGCCTGATGCGACGCATAACCGACTAAACACGCCTACa                                                                                                                             >  1:292486/1‑148 (MQ=17)
          gCACCATTGCCTGATGCGACGCTTACCCGTCTTATCAGGCCTACAAACTTGTGCCTGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATCCGAGCACCATTGCCTGATGCGACGCTTACGCGTCTTATCAGGCCTACAAAc                                                                                                                          <  3:148692/149‑1 (MQ=255)
            aCCATTGCCTGATGCGACGCTTACCCGTCTTATCAGGCCTACAAACTTGTGCCTGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATCCGAGCACCATTGCCTGATGCGACGCTTACGCGTCTTATCAgg                                                                                                                                   >  2:197400/1‑138 (MQ=255)
             ccATTGCCTGATGCGACGCTTATCCGTCTTATCACGCCTACAAACTTGTGCCTGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATCCGAGCACCATTGCCTGATGCGACGCTTACGCGTCTTATCAGGCCTACAAACCtg                                                                                                                       <  4:205101/149‑1 (MQ=255)
              cATTGCCTGATGCGACGCTTACCCGTCTTATCAGGCCTACAAACTTGTGCCTGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATCCGAGCACCATTGCCTGATGCGACGCTTACGCGTCTTATCATGCCTACAAACCtgt                                                                                                                      >  2:218889/1‑149 (MQ=255)
              cATTGCCTGATGCGACGCTTACCCGTCTTATCAGGCCTACAAACTTGTGCCTGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATCCGAGCACCATTGCCTGATGCGACGCTTACGCGTCTTATCAGGCCTACAAACCtgt                                                                                                                      >  1:136020/1‑149 (MQ=255)
              cATTGCCTGATGCGACGCTTACCCGTCTTATCAGGCCTACAAACTTGTGCCTGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATCCGAGCACCATTGCCTGATGCGACGCTTACGCGTCTTATCAGGCCTACAAACCtgt                                                                                                                      >  6:17618/1‑149 (MQ=255)
               cttGCCTGCCGCGACGCTTACCCGTCTAATCACGCCTACAAACTTGTGCCCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATCCGAGCACCATTGCCTGATGCGACGCTTACGCGTCTTATCAGGCCTACAAACCtgtg                                                                                                                     <  2:352353/148‑1 (MQ=21)
                   ccTGATGCGACGCTTACCCGTCTTATCAGGCCTACAAACTTGTGCCTGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATCCGAGCACCATTGCCTGATGCGACGCTTACGCGTCTTATCAGGCCTACAAACCTGTGCCt                                                                                                                  >  8:290450/1‑148 (MQ=255)
                           gACGCTTACCCGTCTTATCAGGCCTACAAACTTGTGCCTGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATCCGAGCACCATTGCCTTCTGCGACGCTTACGCGTCTTATCAGGCCTACAACCCTGTTCCTGCACCGTAg                                                                                                         >  4:40581/1‑149 (MQ=21)
                              gCTTACCCGTCTTATCACGCCGACAAACTTCTGCCTGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATCCGAGCACCATTGCCTGATGCGACGCTTACGCGTCTTATCAGGCCTACAAACCTGTGCCTGAACCGTAGGcc                                                                                                      <  6:242763/149‑1 (MQ=255)
                                ttACCCGTCTTATCAGGCCTACAAACTTGTGCCTGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATCCGAGCACCATTGCCTGATGCGACGCTTACGCGt                                                                                                                                            <  4:348643/109‑1 (MQ=255)
                                ttACCCGTCTTATCAGGCCTACAAACTTGTGCCTGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATCCGAGCACCATTGCCTGATGCGACGCTTACGCGt                                                                                                                                            >  3:348643/1‑109 (MQ=255)
                                    ccGTCTTATCAGGCCTACAAACTTGTGCCTGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATCCGAGCACCATTGCCTGATGCGACGCTTACGCGTCTTATCAGGCCTACAAACCTGTGCCTGAACCGTAGGCCGGAt                                                                                                  >  2:398232/1‑147 (MQ=255)
                                      gTCTTATCAGGCCTACAAACTTGTGCCTGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATCCGAGCCCCATTGCCTGATGCGACGCTTACGCGTCTTATCAGGCCTACAAACCTGTGCCTGAACCGTAGGCCGGATAAgg                                                                                              >  2:240539/1‑149 (MQ=255)
                                      gTCTTATCAGGCCTACAAACTTGTGCCTGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATCCGAGCACCATTGCCTGATGCGACGCTTACGCGTCTTATCAGGCCTACAAACCTGTGCCTGAACCGTAGGCCGGATAAgg                                                                                              >  8:172017/1‑149 (MQ=255)
                                       tCTTATCAGGCCTACAAACTTGTGCCTGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATCCGAGCACCATTGCCTGATGCGACGCTTACGCGTCTTATCAGGc                                                                                                                                  >  2:294675/1‑112 (MQ=255)
                                       tCTTATCAGGCCTACAAACTTGTGCCTGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATCCGAGCACCATTGCCTGATGCGACGCTTACGCGTCTTATCAGGc                                                                                                                                  <  1:294675/112‑1 (MQ=255)
                                         ttATCAGGCCTACAAACTTGTGCCTGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATCCGAGCACCATTGCCTGATGCGACGCTTACGCGTCTTATCAGGCCTACAAACCTGTGCCTGAACCGTAGGCCGGATAAGGCGt                                                                                           >  4:371439/1‑149 (MQ=255)
                                            tCAGGCCTACAAACTTGTGCCTGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATCCGAGCACCATTGCCTGATGCGACGCTTACGCGTCTTATCAGGCCTACAAACCtgtg                                                                                                                     <  5:258107/120‑1 (MQ=255)
                                            tCAGGCCTACAAACTTGTGCCTGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATCCGAGCACCATTGCCTGATGCGACGCTTACGCGTCTTATCAGGCCTACAAACCtgtg                                                                                                                     >  6:258107/1‑120 (MQ=255)
                                            tCAGGCCTACAAACTTGTGCCTGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATCCGAGCACCATTGCCTGATGCGACGCTTACGCGTCTTATCAGGCCTACAAACCTGTGCCTGAACCGTAGGCCGGATAAGGCGTTTa                                                                                        >  5:4538/1‑149 (MQ=255)
                                            tCAGGCCTACAAACTTGTGCCTGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATCCGAGCACCATTGCCTGATGCGACGCTTACGCGTCTTATCAGGCCTACAAACCTGTGCCTGAACCGTAGGCCGGATAAGGCGTTTa                                                                                        <  7:290450/149‑1 (MQ=255)
                                               gcccTACAAACCTGTGCCCGAACCGTCCGCCGGATAAGGCGTTTACGCCGCATCCGGCATCCGAGCACCATTGCCTGATGCGACGCTTACGCGTCTTATCAGGCCTACAAACCTGTGCCAGAACCGTAGGCCGGATAAGGCGTTTAcgc                                                                                     <  8:151749/147‑1 (MQ=17)
                                                gCCTACAAACTTGTGCCTGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATCCGAGCACCATTGCCTGATGCGACGCTTACGCGTCTTATCAGGCCTACAAACCTGTGCCTGAACCGTAGGCCGGATAAGGCGTTTAcgcc                                                                                    <  7:166740/149‑1 (MQ=255)
                                                  cTACAAACTTGTGCCTGAACCGTAGGCAGGATAAGGCGTTTACGCCGCATCCGGCATCCGAGCACCATTGCCTGATGCGACGCTTACGCGTCTTATCAGGCCTACAAACCTGTGCCTGAACCGTAGGCCGGATAAGGCGTTTAcgccg                                                                                   >  1:14311/1‑148 (MQ=255)
                                                   tACAAACTTGTGCCTGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATCCGAGCACCATTGCCTGATGCGACGCTTACGCGTCTTATCAGGCCTACAAACCTGTGCCTGAACCGTAGGCCGGATAAGGCGTTTACGCCGCa                                                                                 >  6:270710/1‑149 (MQ=255)
                                                    aCAAACTTGTGCCTGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATCCGAGCACCATTGCCTGATGCGACGCTTACGCGTCTTATCAGGCCTACAAACCTGTGCCTGAACCGTAGGCCGGATAAGGCGTTTACGCCGCAt                                                                                >  2:391603/1‑149 (MQ=255)
                                                     aaaaCTTGTGCCTGCACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATCCGAGCACCATTGCCTGATGCGACGCTTACGCGTCTTATCAGGCCTACAAACCTGTGCCTGACCCGTAGGCCGGATAAGGCGTTTACGCCGCATc                                                                               <  1:281960/148‑1 (MQ=255)
                                                        aCTTGTGCCTGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATCCGAGCACCATTGCCTGATGCGACGCTTACGCGTCTTATCAGGCCTACAAACCTGTGCCTGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCgg                                                                            >  1:202491/1‑149 (MQ=255)
                                                          ttGTGCCTGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATCCGAGCACCATTGCCTGATGCGACGCTTACGCGTCTTATCAGGCCTACAAACCTGTGCCTGAACCGTAGGCCGGATAAGGCGTTTACGCCCCATCCgg                                                                            >  3:5157/1‑147 (MQ=255)
                                                          ttGTGCCTGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATCCGAGCACCATTGCCTGATGCGACGCCTACGCGTCTTATCAGGCCTACAAACCTGTGCCTGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCgg                                                                            >  6:65493/1‑147 (MQ=255)
                                                            gtgCCTGAACCGTGGGCCGGATAAGGCGTTTACGCCGCATCCGGCATCCGAGCACCATTGCCTGATGCGACGCTTACGCGTCTTATCAGGCCTACAAACCTGTGCCAGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGt                                                                        >  5:55806/1‑149 (MQ=255)
                                                            gtgCCTGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATCCGAGCACCATTGCCTGATGCGACGCTTACGCGTCTTATCAGGCCTACAAACCTGTGCCTGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAg                                                                         <  2:136020/148‑1 (MQ=255)
                                                             tgCCTGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATCCGAGCACCATTGCCTGATGCGACGCTTACGCGTCTTATCAGGCCTACAAACCTGTGCCTGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGt                                                                        <  1:240539/148‑1 (MQ=255)
                                                               acTGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATCCGAGCACCATTGCCTGATGCGACGCTTACGCGTCTTATCAGGCCTACAAACCTGTGCCTGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTCAt                                                                     <  3:294696/148‑1 (MQ=255)
                                                                    aCCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATCCGAGCACCATTGCCTGATGCGACGCTTACGCGTCTTATCAGGCCTACAAACCTGTGCCTGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTCATGCGTc                                                                >  3:2986/1‑149 (MQ=255)
                                                                      cGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATCCGAGCACCATTGCCTGATGCGACGCTTACGCGTCTTATCAGGc                                                                                                                                  >  4:230070/1‑81 (MQ=38)
                                                                      cGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATCCGAGCACCATTGCCTGATGCGACGCTTACGCGTCTTATCAGGc                                                                                                                                  <  3:230070/81‑1 (MQ=38)
                                                                        tAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATCCGAGCACCATTGCCTGATGCGACGCTTACGCGTCTTATCAGGCCTACAAACCTGTGCCTGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTCATGCGTCGATg                                                            <  5:270710/149‑1 (MQ=255)
                                                                         aGGCCGGATAAGGCGTTTACGCCGCATCCGGCATCCGAGCACCATTGCCTGATGCGACGCTTACGCGTCTTATCAGGCCTACAAACCTCTGc                                                                                                                    <  8:362245/92‑1 (MQ=255)
                                                                          ggCCGGATAAGGCGTTTACGCCGCATCCGGCATCCGAGCACCATTGCCTGATGCGACGCTTACGCGTCTTATCAGGCCTACAAACCTGTGCCTGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTCATGCGTCGATGc                                                           <  1:218889/148‑1 (MQ=255)
                                                                           gCCGGATAAGGCGTTTACGCCGCATCCGGCATCCGAGCACCATTGCCTGATGCGACGCTTACGCGTCTTATCAGGCCTACAAACCTGTGCCTGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTCATGCGTCGATGcc                                                          >  7:167062/1‑148 (MQ=255)
                                                                            ccGGATAAGGCGTTTACGCCGCATCCGGCATCCGAGCACCATTGCCTGATGCGACGCTTACGCGTCTTATCAGGCCTACAAACCTGTGCCTGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTCATGCGTCGAt                                                             >  4:293559/1‑144 (MQ=255)
                                                                            ccGGATAAGGCGTTTACGCCGCATCCGGCATCCGAGCACCATTGCCTGATGCGACGCTTACGCGTCTTATCAGGCCTACAAACCTGTGCCTGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTCATGCGTCGAt                                                             <  3:293559/144‑1 (MQ=255)
                                                                             cGGATAAGGCGTTTACGCCGCATCCGGCATCCGAGCACCATTGCCTGATGCGACGCTTACGCGTCTTATCAGGCCTACAAACCTGTGCCTGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTCATGCGTCGATGCCaaa                                                       >  8:11642/1‑149 (MQ=255)
                                                                                     gCGTTTACGCCGCATCCGGCATCCGAGCACCATTGCCTGATGCGACGCTTACGCGTCTTATCAGGCCTACAAACCTGTGCCTGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTCATGCGTCGATGCCAAATGCGCCAc                                               >  7:95303/1‑149 (MQ=255)
                                                                                           aCGCCGCATCCGGCATCCGAGCACCATTGCCTGATGCGACGCTTACGCGTCTTATCAGGCCTACAAACCTGTGCCTGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTCATGCGTCGATGCCAAATGCGCCACCCTa                                           >  5:172503/1‑147 (MQ=255)
                                                                                            cgccgcATCCGGCATCCGAGCACCATTGCCTGATGCGACGCTTACGCGTCTTATCAGGCCTACAAACCTGTGCCTGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTCATGCGTCGATGCCAAATGCGCCACCCTaaa                                         <  3:258387/148‑1 (MQ=255)
                                                                                            cgccgcATCCGGCATCCGAGCACCATTGCCTGATGCGACGCTTACGCGTCTTATCAGGCCTACAAACCTGTGCCTGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTCATGCGTCGATGCCAAATGCGCCACCCTAAag                                        >  4:66874/1‑149 (MQ=255)
                                                                                             gccgcATCCGGCATCCGAGCACCATTGCCTGATGCGACGCTTACGCGTCTTATCAGGCCTACAAACCTGTGCCTGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTCATGCGTCGATGCCAAATGCGCCACCCTAAag                                        >  3:86197/1‑148 (MQ=255)
                                                                                               cgcATCCGGCATCCGAGCACCATTGCCTGATGCGACGCTTACGCGTCTTATCAGGCCTACAAACCTGTGCCTGAACCGTAGGCCGGATAAGGCGTTTACGCCGCCTCCGGCAGTCATGCGTCGATGCCAAATGCGCCCCCCTAAagcag                                     >  5:75096/1‑149 (MQ=255)
                                                                                                      ggcatcctAGCACCATTGCCTGATGCGACGCTTACGCGTCTTATCAGGCCTACAAACCTGTGCCTGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTCATGCGTCGATGCCAAATGCGCCACCCTAAAGCAGCGCATcc                              <  6:4538/149‑1 (MQ=255)
                                                                                                             gAGCACCATTGCCTGATGCGACGCTTACGCGTCTTATCAGGCCTACAAACCTGTGCCTGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTCATGCGTCGATGCCAAATGCGCCACCCTAAAGCAGCGCATCCGCAatga                       >  7:137543/1‑149 (MQ=255)
                                                                                                                 aCCATTGCCTGATGCGACGCTTACGCGTCTTATCAGGCCTACAAACCTGTGCCTGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTCATGCGTCGATGCCAAATGCGCCACCCTAAAGCAGCGCATCCGCAATGATGTa                   <  4:2986/149‑1 (MQ=255)
                                                                                                                 aCCATTGCCTGATGCGACGCTTACGCGTCTTATCAGGCCTACAAACCTGTGCCTGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTCATGCGTCGATGCCAAATGCGCCACCCTAAAGCAGCGCATCCGCAATGATGTa                   >  6:334583/1‑149 (MQ=255)
                                                                                                                  ccATTGCCTGATGCGACGCTTACGCGTCTTATCAGGCCTACAAACCTGTGCCTGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTCATGCGTCGATGCCAAATGCGCCACCCTAAAGCAGCGCATCCGCAATGATGTAc                  <  4:86197/149‑1 (MQ=255)
                                                                                                                   cATTGCCTGATGCGACGCTTACGCGTCTTATCAGGCCTACAAACCTGTGCCTGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTCATGCGTCGATGCCAAATGCGCCACCCTAAAGCAGCGCATCCGCAATGATGTACt                 >  5:113254/1‑149 (MQ=255)
                                                                                                                     ttGCCTGATGCGACGCTTACGCGTCTTATCAGGCCTACAAACCTGTGCCTGACCCGTAGGCCGGATAAGGCGTTTACGCCGAATCCGGCAGTCATGCGTCGATGCCAAATGCGCCACCCTAAAGCAGCGCATCCGCAATGATGTACTTa               >  1:260891/1‑149 (MQ=255)
                                                                                                                      ggCCTGATAAGACGCGTAAGCGTCGCATCAGGCCTACAAACCTGTGCCTGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTCATGCGTCGATGCCAAATGCGCCACCCTAAAGCAGCGCATCCGCAatgatg                     <  8:372524/141‑1 (MQ=25)
                                                                                                                      ggCCTGATAAGACGCGTAAGCGTCGCATCAGGCCTACAAACCTGTGCCTGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTCATGCGTCGATGCCAAATGCGCCACCCTAAAGCAGCGCATCCGCAatgatg                     >  7:372524/2‑142 (MQ=25)
                                                                                                                       gCCTGATGCGACGCTTACGCGTCTTATCAGGCCTACAAACCTGTGCCTGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTCATGCGTCGATGCCAAATGCGCCACCCTAAAGCAGCGCATCCGCAATGATGTACTTAt              <  2:202491/148‑1 (MQ=255)
                                                                                                                        ccTGATGCGACGCTTACGCGTCTTATCAGGCCTACAAACCTGTGCCTGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTCATGCGTCGATGCCAAATGCGCCACCCTAAAGCAGCGCATCCGCAATGATGTACTTAtt             <  5:308297/148‑1 (MQ=255)
                                                                                                                         cagATGCGACGCTTACGCGTCTTATCAGGCCTACAAACCTGTGCCTGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACTCATGCGTCGATGCCAAATTCGCCACCCTAAAGCAGCGCATCCGCAATCATGTACTTATTcc           >  2:98079/3‑149 (MQ=255)
                                                                                                                         cTGATGCGACGCTTACGCGTCTTATCAGGCCTACAAACCTGTGCCTGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTCATGCGTCGATGCCAAATGCGCCACCCTAAAGCAGCGCATCCGCAATGATGTACTTATTcc           >  4:358799/1‑149 (MQ=255)
                                                                                                                          tGATGCGACGCTTACGCGTCTTATCAGGCCTACAAACCTGTGCCTGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTCATGCGTCGATGCCAAATGCGCCACCCTAAAGCAGCGCATCCGCAATGATGTACTTATTc            >  6:49053/1‑147 (MQ=255)
                                                                                                                          tGATGCGACGCTTACGCGTCTTATCAGGCCTACAAACCTGTGCCTGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTCATGCGTCGATGCCAAATGCGCCACCCTAAAGCAGCGCATCCGCAATGATGTACTTATTc            <  5:49053/147‑1 (MQ=255)
                                                                                                                            aTGCGACGCTTACGCGTCTTATCAGGCCTACAAACCTGTGCCTGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTCATGCGTCGATGCCAAATGCGCCACCCTAAAGCAGCGCATCCGCAATGATGTACTTATTCCtt         <  2:14311/148‑1 (MQ=255)
                                                                                                                             tGCGACGCTTACGCGTCTTATCAGGCCTACAAACCTGTGCCTGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTCATGCGTCGATGCCAAATGCGCCACCCTAAAGCAGCGCATCCGCAATGATGTACTTATTCCTTCg       <  1:398232/149‑1 (MQ=255)
                                                                                                                             tGCGACGCTTACGCGTCTTATCAGGCCTACAAACCTGTGCCTGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTCATGCGTCGATGCCAAATGCGCCACCCTAAAGCAGCGCATCCGCAATGATGTACTTATTCCTTCg       <  1:391603/149‑1 (MQ=255)
                                                                                                                             tGCGACGCTTACGCGTCTTATCAGGCCTACAAACCTGTGCCTGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTCATGCGTCGATGCCAAATGCGCCACCCTAAAGCAGCGCATCCGCAATGATGTACTTATTCCTTCg       <  5:17618/149‑1 (MQ=255)
                                                                                                                               cGACGCTTACGCGTCTTATCAGGCCTACAAACCTGTGCCTGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTCATGCGTCGATGCCAAATGCGCCACCCTAAAGCAGCGCATCCGCAATGATGTACTTATTCCTTCgg      <  1:250906/148‑1 (MQ=255)
                                                                                                                                 aagCTTACGCGTCTTATCAGGCCTACAAACCTTGGCCGGAACCGTACGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTCCAGCGGCCTTGCCAAAAGCGCCACCCTAAAGCAGCGCATCCGCAATGATGTACTTATTCCTTCGGttt   <  8:137543/147‑1 (MQ=25)
                                                                                                                                  cGCTTACGCGTCTTATCAGGCCTACAAACCTGTGCCTGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATcc                                                                              <  3:175377/73‑1 (MQ=39)
                                                                                                                                  cGCTTACGCGTCTTATCAGGCCTACAAACCTGTGCCTGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATcc                                                                              >  4:175377/1‑73 (MQ=39)
                                                                                                                                   gCTTACGCGTCTTATCAGGCCTACAAACCTGTGCCTGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTCATGCGTCGATGCCAAATGCGCCACCCTAAAGCAGCGCATCCGCAATGATGTACTTATTCCTTCGGTTTa  >  1:318585/1‑148 (MQ=255)
                                                                                                                                        |                                                                                                                                              
CGGCATCCGAGCACCATTGCCTGATGCGACGCTTACCCGTCTTATCAGGCCTACAAACTTGTGCCTGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATCCGAGCACCATTGCCTGATGCGACGCTTACGCGTCTTATCAGGCCTACAAACCTGTGCCTGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTCATGCGTCGATGCCAAATGCGCCACCCTAAAGCAGCGCATCCGCAATGATGTACTTATTCCTTCGGTTTA  >  NC_000913/508612‑508890

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: